ObjectiveTo investigate and analyze an outbreak of rotavirus infectious diarrhea in a prison in Chongqing Municipality, to provide a basis for adult rotavirus surveillance and prevention, and to explore the public health problems in special settings. MethodsA retrospective survey was conducted to collect and analyze data on individual cases with diarrheal disease on-site. The clinical characteristics, as well as the temporal, spatial and geographical distribution patterns of the epidemic were described. Multi-pathogen detection tests were conducted both on diarrhea cases and environmental samples, with viral genotyping performed on positive samples. A case-control analysis was performed to identify the causes of the outbreak, and an SEIR model was adopted to predict the outbreak trend and evaluate the effectiveness of interventions. ResultsA total of 65 cases were found among the inmates, with an attack rate of 2.03%. The predominant clinical manifestations included diarrhea (89.23%), watery stool (73.85%), and dehydration (18.46%). The epidemic curve indicated a “human-to-human” transmission pattern, with an average incubation period of 5‒6 days. The attack rates among chefs in the main canteen (80.00%, 8/10) and caterers (28.33%, 17/60) were significantly higher than those of other inmates （P<0.05）. Multi-pathogen polymerase chain reaction (PCR) testing detected positive for group A rotavirus, with the viral genotyping identified as G9P [8] strain. Factors such as unprotected "bare-handed" food distribution among cases with diarrhea (OR=9.512, 95%CI: 4.261‒21.234) and close contact with diarrhea cases (OR=3.656, 95%CI: 1.719‒7.778) were the possible cause of the outbreak. The SEIR model (r₀=5, α=0.3, β₁=0.08, β₂=0.04) was constructed using prison inmates as susceptible population, aiming at fitting the initial transmission trend of the outbreak, and the epidemic rate declined rapidly after intervention measures were implemented (r_t≈0). ConclusionThis rare rotavirus infection diarrhea outbreak among adults in confined settings suggests that the construction of public health prevention and control systems in prison may be overlooked. Cross infection during meal processing and distribution in the canteens of such settings is likely to be the cause of the outbreak. Given the potential neglect of public heath system construction in special settings, it is imperative to enhance the surveillance and monitoring of rotavirus and other intestinal multi-pathogens among adults, as well as the construction of public health prevention and control systems in these special settings.

Homo- or heterodimeric compounds that affect dimeric protein function through interaction between monomeric moieties and protein subunits can serve as valuable sources of potent and selective drug candidates. Here, we screened an in-house dimeric natural product collection, and panepocyclinol A (PecA) emerged as a selective and potent STAT3 inhibitor with profound anti-tumor efficacy. Through cross-linking C712/C718 residues in separate STAT3 monomers with two distinct Michael receptors, PecA inhibits STAT3 DNA binding affinity and transcription activity. Molecular dynamics simulation reveals the key conformation changes of STAT3 dimers upon the di-covalent binding with PecA that abolishes its DNA interactions. Furthermore, PecA exhibits high efficacy against anaplastic large T cell lymphoma in vitro and in vivo, especially those with constitutively activated STAT3 or STAT3^Y640F. In summary, our study describes a distinct and effective di-covalent modification for the dimeric compound PecA to disrupt STAT3 function.

Sanming launched substantial healthcare system reform in 2012,deeply recognizing that healthcare service price reform is an important healthcare system reform.Combined with the actual situation,Sanming systematically pushes forward the reform of medical service prices,dynamic adjustment and linkage reform,and explores the way that conforms to its own development,and has achieved better results.It primarily discusses the approaches,development outcomes,experiences,and insights of Sanming's healthcare service price reform.

Sanming launched substantial healthcare system reform in 2012,deeply recognizing that healthcare service price reform is an important healthcare system reform.Combined with the actual situation,Sanming systematically pushes forward the reform of medical service prices,dynamic adjustment and linkage reform,and explores the way that conforms to its own development,and has achieved better results.It primarily discusses the approaches,development outcomes,experiences,and insights of Sanming's healthcare service price reform.

Sanming launched substantial healthcare system reform in 2012,deeply recognizing that healthcare service price reform is an important healthcare system reform.Combined with the actual situation,Sanming systematically pushes forward the reform of medical service prices,dynamic adjustment and linkage reform,and explores the way that conforms to its own development,and has achieved better results.It primarily discusses the approaches,development outcomes,experiences,and insights of Sanming's healthcare service price reform.

Sanming launched substantial healthcare system reform in 2012,deeply recognizing that healthcare service price reform is an important healthcare system reform.Combined with the actual situation,Sanming systematically pushes forward the reform of medical service prices,dynamic adjustment and linkage reform,and explores the way that conforms to its own development,and has achieved better results.It primarily discusses the approaches,development outcomes,experiences,and insights of Sanming's healthcare service price reform.

Sanming launched substantial healthcare system reform in 2012,deeply recognizing that healthcare service price reform is an important healthcare system reform.Combined with the actual situation,Sanming systematically pushes forward the reform of medical service prices,dynamic adjustment and linkage reform,and explores the way that conforms to its own development,and has achieved better results.It primarily discusses the approaches,development outcomes,experiences,and insights of Sanming's healthcare service price reform.

Sanming launched substantial healthcare system reform in 2012,deeply recognizing that healthcare service price reform is an important healthcare system reform.Combined with the actual situation,Sanming systematically pushes forward the reform of medical service prices,dynamic adjustment and linkage reform,and explores the way that conforms to its own development,and has achieved better results.It primarily discusses the approaches,development outcomes,experiences,and insights of Sanming's healthcare service price reform.