Objective:To observe the efficacy and safety of high dose methylprednisolone in the pulse therapy for infantile spasms. Methods:Totally 70 cases of infantile spasms were randomly divided into the conventional dose group and high dose group with 35 ones in each according to the random number table. High dose group was given methylprednisolone pulse therapy for the first 5 days, and then received the conventional dose treatment. The conventional dose group received prednisone tablets orally. The treatment course was 8 weeks. The ECG outcome, efficacy and incidence of adverse reactions were observed and compared between the groups, and the efficacy of pulse therapy for the patients with different causes and different course was also studied. Results:The total effective rate of high dose group was 94. 29%, which was significantly higher than that of conventional dose group (71. 43%), and the difference was statistically significant (P<0. 05). The EEG outcome between the groups had not statistical significance (P>0. 05). The incidence of adverse reactions in high dose group was wigher, while the difference was not significant (P>0. 05). The efficacy of pulse therapy for different causes and different course had no statistically significant difference (P >0.05). Conclusion: High dose methylpred-nisolone pulse therapy can effectively improve clinical efficacy for infantile spasms, which shows no effect on the safety of patients.

Objective To discuss the confirmed value of HCV Different Region ELISA KIT (ANTI-HCV-C, ANTI-HCV-NS3, ANTI-HCV-NS4, ANTI-HCV-NS5) to anti-HCV CO sample.Methods To inspect the general antibody of anti-HCV with ELISA, which is commonly used in clinic.The serum was separated from the suspicious samples, whose results were equal to S/CO or in the state of grey district ( CO?10%), and stored in the refrigerator in -29℃ low temperature and concenratedly inspected by the HCV Different Region ELISA KIT on the Triturus,the enayme immuno-reactions automatic analyzer. We totally collected 44 suspicious samples,among which there were 24 anti-HCV positive,20 anti-HCV CO?10% negative. Meanwhile we collected 30 samples whose S/CO of general antiboby was more than 5.0 and 20 ones which were healthy and could be compared with.Results In the 44 suspicious cases, whose S/CO was in the state of grey disrtict (CO?10%), the HCV Different Region Kit confirmed positive was 22.7% (10/44),suspicious 6.8% (3/44), negative 70.5 % (31/44).The false positive rate of positive anti-HCV CO?10% was 54.2%(13/24) and the false negative rate of negative anti-HCV CO?10% was 5.0%(1/20). In the 30 cases,whose S/CO of general antibody was more than 5.0,the HCV Different Region Kit confirmed positive was 80.0%(24/30),suspicious 13.3%(4/30), negative 67%(2/30). In 20 cases,which were from healthy people, the HCV Different Region Kit confirmed totally negative.Conclusion Using HCV Different Region Kit is a better evidence laboratory,especially for the uncertain samples,that is,it is very important for the "grey district"(CO?10%) samples to be further confirmed.It can reduce the false positive and false negative result which may emerge when anti HCV EIA inspects the dangerous groups and improve the accuracy of clinical HCV diagnosis.To blood station, it has certain practical value for the blood not only can be safely used, but also avoided wasting. The operation is simple and convenient, easily be spread and applied.

0.05 ) respectively. Before and after irradiation in the group of 100 mJ, the ratio of G 2+ /P 2+ was 1.667?0.128 and 2.135?0.156( P 0.05 ). Conclusion: The normal pulsed Nd:YAG laser irradiation alone can not increase acid residence of the enamel.

Objective To retrospectively analyze the ultrasonic imaging features and clinical out-comes of biliary complications after percutaneous ablation for hepatic malignant tumors .Methods The cli-nical data of patients with postablation biliary complications who underwent percutaneous ablation for hepatic malignant tumors in the past 7 years in our department were retrospectively analyzed .The ultrasonic imaging features and the clinical outcomes of biliary complications on follow-up of these patients were analyzed .Re-sults Postablation biliary complications occurred in 65 patients , including 34 patients with bile duct dilata-tion and 31 patients with biloma .In patients with bile duct dilatation , the dilated bile ducts were located dis-tal to the ablation lesions in all these patients .Among 30 patients with bile duct dilatation , the dilatation was progressive in 9 patients (9/30, 30.0%) and stable in 20 patients (20/30, 66.7%), while in one patient (1/30, 3.3%) it resolved on follow-up.The radiologic features of bilomas mainly manifested as four types:crescent , interspersed , circumferential and solitary nodular , and the number of patients with the four types were 13 (13/31, 41.9%), 13(13/31, 41.9%), 3(3/31, 9.7%) and 2(2/31, 6.5%), respectively. In 6 patients (6/31, 19.4%) the bilomas were subjected to interventional or surgical therapy while for the remaining 25 patients (6/31, 19.4%) they were treated conservatively .Three (3/25, 12.0%) of the lat-ter 25 patients had spontaneously disappearance of the biloma and 2 (2/25, 8.0%) of the remaining 22 pa-tients appeared to have progressive increase in size .In 17 (17/25, 68.0%) patients the biloma remained stable in size and in the remaining 3 patients (3/25, 12.0%) the bilomas resolved.Conclusions Postab-lation biliary complications of ultrasound-guided percutaneous ablation for hepatic malignant tumors mainly presented as biloma and bile duct dilatation .Dilated bile ducts were located in the distal side of the ablation zones.Crescent type and interspersed type were the most common morphological types of biloma .Although most patients with postablation biliary complications of ultrasound-guided percutaneous ablation remained sta-ble or resolved on follow-up, interventional or surgical therapy was necessary in the minority of patients .

Objective To explore the effect of Buyanghuanwu decoction on blood glucose control and renal function protection in patients with early diabetic nephropathy.Methods 170 cases of patients with early diabetic nephropathy from May 2012 to June 2013 in wenzhou hospital of integrated traditional chinese and western medicine were selected,all patients were divided into observation group and control group,the control group was given routine treatment,the observation group treatment with Buyanghuanwu decoction based on control group .The clinical effective rate was compared between the two groups after treatment,the levels of blood lipids, 24 h urine microalbumin and hemorheology were compared between the two groups before and after treatment.Results After treatment, the total effective rate of the observation group was 90.59%,which was significantly higher than the control group 76.47% (P<0.05).Compared with before treatment,the levels of triglycerides and cholesterol decreased in two groups,high-density lipoprotein levels increased,24 h urine microalbumin decreased,whole blood viscosity,whole blood viscosity trimming and fibrinogen decreased,the difference was statistically significant(P<0.05).Compared with the control group,the levels of triglycerides and cholesterol in observation group was lower,high-density lipoprotein was higher,24 h urine microalbumin was lower,whole blood viscosity,whole blood viscosity trimming and fibrinogen were lower,the difference was statistically significant(P<0.05).Conclusion Buyanghuanwu decoction in the treatment of diabetic nephropathy is effective in treating diabetic nephropathy,it can effectively reduce blood lipids and blood viscosity,significantly reduce urinary protein excretion and protect the kidneys.

Objective To observe the preventive effects of 2.5% sodium bicarbonate solution on the patients who had upper respiratory tract infection caused by fungal with hormone treatment.Methods 36 patients of systemic lupus erythematosus,polymyositis/dermatomyositis were randomly divided into two groups:observer group with glucocorticoid treatment and using 2.5% sodium bicarbonate solution to clean the mouth,and control group only with glucocorticoid treatment.After 7 days,we observed the rate of fungal infection of the two groups.Results There was significant diffience on the rate of fungal infection between observer group and control group(P0.05).Conclusion Using 2.5% sodium bicarbonate solution to clean the mouth can effectively prevent the patients who has upper respiratory tract infection caused by fungal with hormone treatment and decrease the rate of fungal infection.

CARMA1, BCL10 and MALT1 are lymphocyte-specific signaling molecules of NF-?B pathway.The abnormalities of those molecules such as gene mutation, rearrangement, translocation or amplification often connect with the lymphoma genesis. This article reviewed the physiological function of those molecules and the relationship between genetic abnormalities and lymphoma genesis, also with present target-treatment research and new gene medicine development.

Background: The efficacy of traditional treatment for irritable bowel syndrome (IBS) is limited. Some small case series published abroad showed that fecal microbiota transplantation (FMT) is effective for treatment-resistant IBS, but studies investigating the effect of FMT on IBS refractory to conventional therapy are rare in China. Aims: To observe the efficacy and safety of FMT in patients with IBS refractory to conventional therapy. Methods: Nineteen inpatients with IBS (including 15 cases of IBS with diarrhea and 4 cases of IBS unclassifiable) at the First Affiliated Hospital of Guangdong Pharmaceutical University who were refractory to conventional treatment for more than 6 months were enrolled in this study. Fecal microbiota was transplanted through colonic or middle digestive tract transendoscopic enteral tubing (TET). The clinical efficacy and safety were evaluated after 1 month and 6 months of treatment. Results: Each IBS patient received 2-4 courses of treatment (totally 186 FMT procedures). The mean value of Bristol stool form scale was 6.00±0.67 before FMT treatment, and decreased to 4.58±0.61 and 4.32±0.58 after 1 month and 6 months of treatment, respectively (P<0.05). The score of IBS severity scoring system (IBS-SSS) decreased from 301.05±58.30 to 208.42±55.10 and 171.58±59.37 after 1 month and 6 months of treatment, respectively (P<0.05). No significant differences in improvement in stool form and clinical symptoms were found between 1 month and 6 months of treatment (P>0.05). The most common adverse effects were transient diarrhea, distension and abdominal pain during the hospitalization. During the 6-month follow-up, no adverse effects and pathogen-related diseases were observed. Conclusions: FMT is effective for the treatment of IBS patients who are refractory to conventional therapy. The adverse effects are mild and transient.

Objective To explore the continuous care needs of stroke patients when discharge,and analyze the related influenced factors.Methods One hundred and twenty cases with first stroke attack were selected,and their continuous care needs were investigated by methods of filling in questionnaires when discharged.The National Institutes of Health Stroke Scale and Barthel Index were applied to assess the severity of acute stroke and their ability of daily life when cases were admitted and discharged,respectively.We investigated the continuous care needs of stroke patients,and analyzed the relationship of that with the severity of acute stroke and their ability of daily life.Results The top five continuous care needs were knowledge of stroke,safety administration,methods of rehabilitation,self control and monitoring blood pressure,and diet direction.Those ratios of needs ranged from 87.25%to 94.12%.The score of continuous care needs had a positive relationship with that of NIHSS(r=0.271,<0.05),and had a negative relationship with that of BI both at the time of discharge(r=-0.226,<0.05).Conclusion The various continuous care needs are very common in patients with first stroke attack for varied reasons.More serious the neurological dysfunction and lower ability of daily life,higher the level of needs for continuous care.

Objective To investigate the relationship between serum nesfatin-1 and gestational diabetes mellitus (GDM). Methods The serum nesfatin-1 concentrations were measured in 45 GDM patients and 44 NGT pregnant women , as well as 47 non-pregnant healthy women served as control. Serum nesfatin-1 were measured by sandwich enzyme linked immunosorbent assay (ELISA). Meanwhile FPG、 lipoid、 FFA、 FINS、 C-tide were also measured. Result Serum nesfatin-1 level in GDM were significantly higher in GDM patients than in NGT and control (P < 0.01), while serum nesfatin-1 in NGT was significantly lower than in control (P < 0.01). Correlation analysis showed that serum nesfatin-1 was positively correlated with FINS , C-tide , HOMA-IR , triglycerides FBG and OGTT 1 hour glucose, HbA1c (P < 0.05). Conclusion Serum nesfatin-1 level in pregnant women significantly decreas, while in GDM patients significantly increase. Serum nesfatin-1 level in pregnant women is positively correlated with Insulin resistance (IR) and triglycerides level.