Objective To study the relationship between plasma level of tissue factor(TF)and severity of coronary heart disease(CHD).Methods Coronary angiography was performed in 237 patients with suspected CHD,with 149 diagnosed as CHD and 88 excluded.Seventy of stenosis of coronary arteries (CA)was evaluated by modified Gensini score on the basis of angiographic imaging.Blood glucose,serum levels of total cholesterol(TC),triglycerides(TG),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),lipoprotein(a)[Lp(a)],uric acid,high sensitivity C-reactive protein(Hs-CRP),fibrinogen(FIB)and plasma level of TF were determined for all the subjects.Relationship between plasma level of TF and severity of pathological changes in CA was evaluated by linear correlation and multiple regression analyses for controlling other relevant factors.Results Plasma level of TF in patients with CHD[(138±39)ng/L]was significantly higher than that in controls[(61±19)ng/L](P＜0.01).Plasma level of TF obviously correlated with severity of pathological changes in CA(r=0.710,P＜0.01).Partial correlation analysis showed a positive correlation between plasma TF and severity of pathological changes in CA,independent of other potential influential factors,with a partial coefficient of correlation of 0.509(P＜0.01).Multiple stepwise regression analysis indicated that plasma level of TF associated with severity of pathological changes in CA,as well as with diabetes,LDL-C,Lp(a),Hs-CRP and FIB,with coefficients of correlation of 0.172,0.180,0.147,0.308 and 0.175,respectively(P＜0.05).Conclusion Elevated plasma level of TF may be one of important risk factors for CHD,which reflects severity of pathological changes in CA.

Objective To explore the correlation between serum cytokines and the depression degree in the patients with colorec-tal cancer.Methods 9 6 cases of colorectal cancer were selected and divided into 2 groups according to the patients health question-naire(PHQ-9):the depression group (52 cases)and the non-depression group (44 cases),and 40 healthy cases were recruited as the control.Serum interleukin-2 (IL-2),soluble interleukin-2 receptor (SIL-2R)and tumor necrosis factor-α(TNF-α)were detected by enzyme-linked immunosorbent assay(ELSIA).The detection results were compared among various groups.And the correlation be-tween the expression of various cytokines and the depression degree was analyzed by the Pearson′s correlation coefficient.Results The levels of IL-2,SIL-2R and TNF-αhad statistical differences among 3 groups (P<0.05);no statistical difference in the TNF-αexpression was noted between the depression and the non-depression groups (P>0.05),but which in these two groups was higher than that in healthy group (P<0.05);the IL-2 level in the depression group was significantly lower than that in the non-depression group(P<0.05);the SIL-2 R level in the depression group was obviously higher than that in the non-depression group (P<0.05), moreover,which in these two groups was higher than that in the healthy group (P<0.05).By correlation analysis,the depression degree in the patients with colorectal cancer was negatively correlated with the IL-2 level (r=-0.259,P<0.05)and positively correlated with the SIL-2R level(r=0.412,P<0.05).Conclusion The levels of IL-2 and SIL-2R have certain correlation with the depression degree in the patients with colorectal cancer,it is speculated that the above cytokines may play a mediating role in the pathogenesis process of depression in the colorectal cancer patients.

0.05);the bacteriological effection rates were 62.50% vs. 40.43%(P

OBJECTIVE: To explore the therapeutic effect of the extracts from Cordyceps militaris on membraneous nephritis model rats.METHODS: Membraneous nephritis model rats were induced by using cationic bovine serum albumin.3 weeks later,urine protein quantity(24 h) was determined weekly;at the end of experiment,the hemorheology,serum creatinine(Scr),blood urea nitrogen(BUN) and serum lipid level were examined and the therapeutic efficacy was evaluated.RESULTS:C.militaris-treatment reduced urine protein quantity(24 h),blood viscosity,serum creatinine(Scr),blood urea nitrogen(BUN) and serum lipid level and delayed the progress of renal lesions.CONCLUSION: Extracts of C.militaris has the action of improving renal function.

The surgery of the breast cancer has dramatically changed,sentinel lymph node(SLN) is one of the aspects.SLN biopsy can precisely predict the status of axillary basin.If the SLN is negative,the patients can spare the unnecessary axillary lymph node dissection(ALND) and reduce the morbidity of the surgery.One of the most important issues with sentinel node biopsy is the intraoperative pathological evaluation of the sentinel nodes.Accurate intraoperative pathological examination of sentinel nodes would enable the selection of candidates for ALND during the initial operation,eliminating the need for second surgery.This article will review the two techniques(touch imprint cytology and frozen section) employed for intraoperative examination of SLN and compare these two methods.And evaluate the role of touch imprint cytology and existing problems in introperative diagnosis of the sentinel lymph nodes and raise the probably ways to improve the sensitivity of touch imprint cytology initially.

AIM: To study factors influencing mannitol to induce blood brain barrier (BBB) opening. METHODS: By constant flow pump, mannitol with different concentration (20% and 25%) was infused into left internal carotid artery of adult Wistar rats via their left external carotid artery at different speed and time. Firstly, after mannitol with a concentration of 25% was transfused, the optimal open time of BBB was investigated using Evans blue as an indicator by injection at different time (0, 2, 4, 6, and 8 min). Then influences of concentration of mannitol, speed of infusion, and change of time on mannitol to induce BBB opening were studied. Finally, death rate was counted according to sex and body weight in order to observe the influence of sex and body weight on tolerance of rats. RESULTS: The open degree of BBB was optimal after 2 min when 3.6 ml mannitol with a concentration of 25% was infused in 30 s at a speed of 0.12 ml?s -1 . However, mannitol with a concentration of 20 % did not almost have any effect on opening of BBB. Slowing of infusion speed or shortening of infusion time of mannitol reduced opening degree of BBB. Body weight affected rat tolerance to mannitol, and the tolerance of the rat was higher in those with higher weight. CONCLUSION: The factors influencing mannitol to induce BBB opening are as follows: concentration, infusion speed, and keeping time of mannitol. And the tolerance to mannitol is not concerned with the sex of the rats, but the body weight of the rats.

: Objective To explore the correlation between serum testosterone and type 2 diabetes mellitus in elderly male.Methods 1012 elderly male were divided into 2 groups according to their histry of DM.Blood glucose(FBG,PBG),cholesterol(CHO),triglyceride(TG),blood pressure(DBP,SBP),body mass index(BMI),serum insulin(FINS,PINS),serum testosterone(T)and estradiol(E2)were measured.Results Eldly male with type 2 diabetes showed higher FBG,PBG,TG,FINS,PINS and E2 level than those without DM(P<0.05),and with lower HDL-C and T level(P<0.05).The analysis of multiple stepwise regression showed that T level was correlated with the level of HDL-C,FINS,PINS and TG.Conclusion TG,HDL-C,FINS and PINS level correlate with T level in eldly male with type 2 diabetes mellitus.

Objective To investigate the effect of Niaoduqing combined with Telmisartan on the diabetic nephropathy in rats. Methods Forty female Wistar rats were randomly divided into the normal control group ( NC ) , the diabetic nephropathy in the control group ( DM ) , the uremic clearance group ( ND ) , and the combination group (LH). Rats in the DM, ND, LH groups were used to establish the diabetic nephropathy model. Rats in each group received the corresponding drugs. Twelve weeks later, the interactions among angiotensin receptor and adiponectin receptor were detected by co-immunoprecipitation assay. Western blot was performed to detect the expression of MCP-1, TGF-βto reveal the injury of the renal tubular interstitial. Results (1)Exogenous co-immunoprecipitation assay indicated that AdipoR1 and AT1, AT2 can form dimerization. Levels of AdipoR1-AT1, AdipoR2-AT2 dimerization were incresaed in the DM group compared with those in the NC group, but were decreased in the ND group and the LH group. (2)MCP-1 and TGF-βprotein expression were increased in the DM group compared with the normal control group (P < 0.05), but were decreased in the ND group and the LH group (P<0.05, respectively). Conclusion Niaoduqing combined with Telmisartan can significantly inhibit the expression of MCP-1 and TGF-βto relieve the renal tubular interstitial injury in rats with diabetic nephropathy. This protective mechanism may associated with the decrease of the dimerizations of AdipoRs and ATs.

Objective To explore the expression and significance of nestin in renal tubular epithelial cells in hypercholesterolemic rats. Methods Dietary-induced hyperlipidemia were induced in female SD rats by given 4% cholesterol and 1% cholic acid diet for 16 weeks. Changes of serum lipid, urinary albumin, serum creatinine and renal interstitial pathological changes were assessed. The expression of nestin and a-smooth muscle actin (α-SMA) were detected by immunohistochemical stain. Results The serum levels of total cholesterol, low density lipoprotein, urinary albumin and serum creatinine were significantly increased in hyperlipidemia group, accompanied with renal interstitial injury and fibrosis. As time extended, the expression of nestin and a-SMA in renal tubular epithelial cells were increased significantly. There was positive correlation among the expression of nestin and total cholesterol, low density lipoprotein, urinary albumin and serum creatinine( r =0.963,0.830,0.944,0.706, P <0.01). Nestin also had a positive correlation with tubular-interstitial index ( r = 0. 974, P < 0. 01) and α-SMA ( r = 0. 804, P < 0. 01). Conclusion The increased expression of nestin may be associated with renal tubular-interstitial fibrosis and tubular epithelial myofibroblast transdifferentiation in hypercholesterolemic rats.

Objective To analyze the expression stability of the three widely used reference genes β-glueuronidase (GUSB), glycera]dehydes-3-phosphate dehydrogenase (GAPDH), β2-microglobulin (β2-M) in Chinese lung cancer tissue specimens and normal lung tissue specimens. Methods Gene expression wasmeasured by quantitative real time PCR and expression stability was analyzed with two widely used softwares genorm and normfinder. Results The intra-and inter-group difference of GAPDH is maximum (The intra and inter-group s is 1.07 and 0.93 respectively, |△ Ct|=2.01±1.06; P =0.000). The mean of these three genes' Ct value is the most stable one analyzed by the two softwares. But the t test showed that the mean of Ct value of GUSB and β2-M is the unique combination that had the minimum intra-and inter-group difference, with no statistically significant differences between normal and malignant samples (The intra-and inter-group s is 0.53 and 0.79 respective]y, |△Ct|= 0.73±0.53; P =0.053). Conclusion It is inappropriate to normalize data derived from lung tissue specimens using one of these three housekeeping genes alone. Among the different combinations of these three genes, the mean of the Ct values of GUSB and β2-M is the best choice as the internal control of lung tissue specimens.