With the understanding of the function mechanism of RNAi,it has been widely applied in various fields.RNAi is a powerful tool to study gene functions and provides a new technical method for the specific gene treatment as well.The further development of RNAi technique opens up a new way for tumor gene treatment.

Influenza A(H1N1)virus is a re-mixed strains of human influenza virus genes,avian influenza virus gene and swine influenza virus gene.Influenza A(H1N1)pandemic influenza has spread around the world,which has drawn worldwide attention.In order to early discovery,early diagnosis,early treatment and effective prevention of Influenza A(H1N1),we describe the characteristics of linfluenza A(H1N1)virus,epidemiology,pathogenesis,clinical manifestations,laboratory examination and effective treatment and preventive measures.

Objective To study the effect of dexmedetomidine combined with ketamine anesthesia on interventional therapy of children with congenital heart disease (CHD). Methods 40 cases of congenital heart disease were treated with catheter intervention from January 2013 to January 2017. The changes of pulmonary arterial pressure and the effect of anesthesia were compared before and after operation. Results 40 cases of children without surgical deaths, 36 cases of successful closure, the success rate of 90% of the operation, the patient after catheter intervention surgery after the results of the test were better than before and after treatment of children with pulmonary artery pressure (P<0.05), and the anesthesia effect was good in children. Conclusion The effect of dexmedetomidine combined with ketamine on the treatment of cardiac catheterization in children with congenital heart disease can achieve good results. The success rate of operation is high and safe, and it is highly reliable.

Objective To compare the effects of the two kinds of crystalloid solutions on glycometabolism in type 2 diabetic patients during the operation for the sclestion of the most suitable crystalloid solution. Methods In the forty scheduled gastrointestinal operation patients,there were twenty (D-group) type 2 diabetic patients and the others (N-group) were non-diabetic patients. Each of the two groups were randomly divided into two groups according to infusing different crystalloid solutions: lactated Ringer’s solution (L group), Plasma Lyte A (A group).So the patients were divided into D-L group,D-A group,N-Lgroup,N-A group.The blood glucose concentration,the blood lactate concentration and artery blood gas analysis before operation (T0) and at the end of operation(T1)were measured . Results The blood glucose concentrations had significant increase at the end of operations,and the blood glucose concentrations of D group were higher than that of N group at T0 and T1.The lactate concentrations before operation were normal in all the patients, but the lactate concentrations were over the normal limits after infusing lactated Ringer’s solution and remained normal after infusing Plasma Lyte A.The lactate concentrations had significant increase in the other three groups except N-A group. Conclusion Plasma Lyte A is the more safe and efficient crystalloid solution for diabetic patients during the operation.

Objective To discuss the efficacy of laparoscopy combined with uterine aspiration for tubal interstitial pregnancy and cornual pregnancy. Methods From January 2004 to January 2007,laparoscopy combined with the preservation of the oviducts was performed on 56 patients with tubal interstitial pregnancy or cornual pregnancy. During the operation,the ectopic pregnancy tissues were removed,and then uterine aspiration was carried out. Results The operation was completed in all of the cases without conversion to open surgery. One of the patients showed persistent ectopic pregnancy,and was cured by muscular injection of MTX injection. In this series,the rate of oviduct patency was 33.9% (19/ 56); 18 moths after the operation,the uterine pregnancy rate was 71.4% (40/56),ectopic pregnancy rate was 16.1%(9/56),and the secondary infertility rate was 1.2% (7/56). Conclusions It is safe and effective to treat tubal interstitial pregnancy or cornual pregnancy with laparoscopic operation combined with uterine aspiration.

Objective To screen the microsatellites with low occurrence rate of stutter band and establish the effective bovine STR typing system.Methods The tetranucleotide STR loci in bovine genome were searched with Tandem Repeat Finder software.Primers were designed and used to amplify these candidate loci and the PCR products were separated with electrophoresis.DNA samples from 100 head of unrelated cattle were typed.Results Among these candidate loci,6 bovine tetranucleotide STR loci showed high polymorphism,and their CDP and CPE value were 0.99995 and 0.859591 respectively.Conclusion The 6 bovine tetranucleotide STR loci can be used for bovine identification and parentage testing.

Objective To evaluate whether glucose is associated with thrombolysis in myocardial infarction (TIMI) flow before primary percutaneous coronary intervention (PCI).Methods Pre-operation blood glucose level, TIMI flow and baseline characteristics of 120 consecutive acute ST-segment elevation myocardial infarction (STEMI) patients treated with primary PCI were analyzed. Hyperglycemia was defined as whole blood glucose ≥7.8 mmol/L (140 mg/dL). Results Only 18 patients (15%) had TIMI 3 flow before primary PCI and hyperglycemia was observed in 87 patients (72.5%). TIMI grade 3 flow were more commonly found in the normoglycemia group compared with the hyperglycemia group before primary PCI (27.3% vs 10.3%,P

Objective To investigate the possible role of valsartan(Val) in transdifferentiation of human renal tubular epithelial cell line(HKC ). Methods HKC cells were divided into four groups: (1) serum-free (negative control); (2) MCP-1 + AAI-treated (positive control); (3) Val-treated alone; (4) Val-inhibition(treated with MCP-1 + AAI + Val). Then the expression of vimentin, ?-smooth muscle actin (?-SMA ) of HKC cells were assessed by indirect enzyme immunohistochemistry (IEI), and the percentage of ?-SMA(+ ) HKC cells was assessed by flow cytometry. Results No difference in expressions of vimentin and ?-SMA by IEI and the percentage of ?-SMA(+ ) HKC cells by cytometry were found between serum-free control HKC cells and Val treated ones. The expressions of vimentin and ?-SMA in positive controls were markedly stronger than negative controls; while these expressions in Val + MCP-1 + AAI-treated HKC cells were less strong than those in positive controls. The percentage of ?-SMA(+ ) HKC cells in the positive controls was significantly higher than that in negative controls(91. 8% vs 3. 1%, P

Objective To explore the management of gliomas in pregnant women.Methods We retrospectively analyzed the clinical data in 11 pregnant women with gliomas treated at Xuanwu Hospital of Capital Medical University between January 1989 and December 2012.Results All the patients were first diagnosed with gliomas during pregnancy,including two cases during early pregnancy,four cases during midpregnancy,and five cases during late pregnancy.The main clinical manifestations were dizziness,headache,nausea and vomiting due to intracranial hypertension,accompanied by epilepsy and blurred vision,hearing and speech disorder and other neurological dysfunctions.Among the 11 patients,one received no obstetric treatment,five underwent cesarean section,three had metaphase induced labor,one had induced abortion,and one had spontaneous abortion.Five neonates appeared normal without malformation:four survived,and one with severe asphyxia died.Ten patients underwent neurosurgery,four of them were diagnosed with low-grade gliomas and six were diagnosed with high-grade glioma by pathology.Seven patients received craniotomy after termination of pregnancy,two underwent craniotomy before termination of pregnancy,and one received emergency craniotomy immediately after admission without obstetric treatment because of her critical conditions.Of the ten patients treated with craniotomy,one died after a coma for 50 days,one was lost to follow-up,six received radiotherapy plus chemotherapy,one received chemotherapy alone,and one received neither radiotherapy nor chemotherapy.Among the 11 patients,one patient who underwent cesarean section without craniotomy was lost to follow-up,one patient with low-grade glioma died 50 days after operation,one treated with craniotomy was lost to follow-up,and eight were followed up for four months to three years,among which four survived and four with high-grade glioma died within one year after operation.Conclusion The overall prognosis of glioma patients during pregnancy is poor.It is important to take into account the tumor status of the pregnant women,gestational age,fetal maturity and patient's desire for tocolysis,and weigh the pros and cons,and timely terminate pregnancy and perform neurosurgery.

Objective To observe efficacy and safety of 2 ways of intravenous anesthesia in endoscopic mucosal resection (EMR) for early esophageal cancer and its precancerous lesion.Methods A total of 140 patients with early esophageal cancer and its precancerous lesion undergoing EMR were enrolled and randomized into 2 groups, with 70 patients in each.Midazolam and propofol were used for group Ⅰ, while midazolam and etomidate were used for group Ⅱ.Vital signs, anesthetic effect, recovery time during the therapy, intra-operative and post-operative adverse reactions were compared.Results Anesthesia with both methods could reach the need for safe EMR.The blood pressure and heart rate of patients in group Ⅰ significantly decreased during the therapy, while no significant difference was found in those of group Ⅱ, but which was significantly different from those of group Ⅰ at some parts of the observation point (P＜0.05).Pulse oxygen saturation of all patients during the procedure was within normal range.No significant difference was found between 2 groups in recovery time.The incidence of muscle tremor or myoclonus in group Ⅱ was significantly higher than that of group Ⅰ, but the incidence of bradycardia and hypotension in group Ⅱ was lower than that of group Ⅰ (P＜0.05).Several patients complained pain, nausea and dizziness after therapy, but there was no significant difference between two groups. Conclusion Anesthesia with propofol and etomidate can both be applied to digestive endoscopy.Etomidate shows advantages in more stable hemodynamics.