A migraine is a common essential headache in clinical practice. Studies in recent years have found that cortical spreading depression (CSD) may be one of the important mechanisms for the cause of migraine. This article reviews the relationship between SCD and neurobiological mechanism of migraine,particular expounding the significance in the gene mutations of familial hemiplegic migraine, CSD in migraine pathophysiological mechanisms and their prophylactic treatment.

B-Raf kinase (BRAF) gene is a driver mutation,and is an effective target in the treatment of non-small cell lung cancer (NSCLC).Studies have shown that BRAF inhibitors are effective for treatment of NSCLC with BRAF mutant.It is important to understand the clinicopathologic features and the research progress of BRAF inhibitors for the individual treatment of NSCLC.

Epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs)and anti-angio-genic drugs have individually demonstrated clinical benefit in the treatment of patients with advanced non-small cell lung cancer (NSCLC).Recent studies demonstrate that the combination of anti-EGFR and anti-angiogene-sis can more significantly enhance clinical benefit,and even can remit EGFR-TKIs resistance in the treatment of advanced NSCLC.According to the different kinds of anti-angiogenesis drugs,recent clinical studies mainly include the combination of anti-vascular endothelial growth factor monoclonal antibody bevacizumab plus EGFR-TKIs and multi-targeted receptor anti-angiogenic tyrosine kinase inhibitor plus EGFR-TKIs,and the for-mer results show a more significant improvement in terms of safety and efficacy in the treatment of advanced NSCLC.Therefore,the combination of bevacizumab plus EGFR-TKIs can be used as a new treatment standard in the treatment of some patients with NSCLC.

Objective:To study the relationship between genetic diversity within S.mutans and dental caries.Methods:Isolates of S.mutans were obtained from 10 caries active children and their mothers,and were originally isolated on mitis-salivarious-bacitracin agar.A total of 800 isolates were biochemically confirmed as S.mutans.Chromosomal DNA was extracted from those isolates with an extraction kit.By AP-PCR,the genetic diversities of S.mutans were examined respectively in 10 caries active children,10 caries-free children,and their mothers.DNA fingerprints were obtained by electrophoresis on 15 g/L agarose gel and analyzed for genotypics similarities.Results:The number of distinct genotypes of S.mutans harbored in caries active children were,on average,greater than that presented in children without caries(P

Background and purpose:Norcantharidin(NCTD)can inhibit the growth of tumors.In this study,we presented the chorioallantoci membrane(CAM)model to evaluate the effect of NTCD on angiogenesis and to study the anti-angiogenesis of NTCD in the chicken embro implant model of human breast carcinoma MCF-7 cells.Methods:The method was based on the implantation of gelatin sponges on top of growing CAM.The sponges were treated with various amounts of NCTD and 0.9% NaCl.Blood vessels surrounding CAM mesenchyme were counted.Then we established the MCF-7 chicken embryo implant model.The chicken embryo CAMs was treated with 0.9% NaCl,Various amounts of NCTD(72,36,18 ?g per 20 ?l)and the inhibition rates were calculated.Results:NCTD can inhibit the new capillary vessels growing into gelatin sponges placed on the CAM in a dose-dependent manner,and the inhibitory rates were 77.7%,62.9%,50.6% and 33.0% respectively.NCTD at the dosages of 72、36、18 ?g were able to inhibit capillary growth regardless of the angiogenic process induced by xenograft tumor and had a significant inhibition as compared with the control group,and the rates were 66.2%,39.3%,22.8% respectively.Conclusions:NCTD has an anti-angiogenic effect for targeting tumor angiogenesis.NCTD may be a potential therapeutic candidate for clinical application.

Purpose:To explore the expression of metastasis suppressor gene Kai1 in ovarian epithelial tumor and its significance.Methods:The expression of Kai1 protein in 108 samples of epithelial ovarian tumors and 12 of normal ovarian tissues was examined by S-P immunohistochemical staining.Results:The positive rates of Kai1 protein expression among malignant,borderline,benign and normal tumors were 26.6%,71.4%,73.3% and 83.3% respectively. The Kai1 protein expression in malignant tumors was obviously less than that in benign ones ( P 0.05). However,there was significant difference in Kai1 protein expression between samples of ovarian epithelial cancers with and without lymphonode metastasis ( P

Objective To investigate the clinical efficacy of edaravone combined with β-aescin sodium for treatment of elderly patients with acute cerebral hemorrhage,and the dynamic changes of tumor necrosis factor-α(TNF-α) and C-reactive protein (CRP),vascular endothelial growth factor (VEGF) levels in serum in such patients before and after treatment.Methods A total of 99 elderly patients with acute cerebral hemorrhage admitted to Henan Nanyang Second General Hospital from April 2014 to February 2016 were enrolled,and they were divided into control group (49 cases) and observation group (50 cases) according to the random digital table.Conventional treatment were given to both groups,patients in the observation group were added with edaravone 30 mg intravenous drip,2 times a day and β-aescin 20 mg intravenous drip,once daily.After treatment for consecutive 2 weeks,the clinical effect and neurological deficit score (NDS) were observed in the two groups;serum CRP levels were detected by immunonephelometry;TNF-αα and VEGF were detected by enzyme linked immunosorbent assay (ELISA).The changes of CRP,VEGF,TNF-α levels and incidence of adverse reactions before and after treatment were compared between the two groups.Results The levels of NDS,CRP,VEGF and TNF-α in both groups after treatment were significantly lower than those before treatment,and the degrees of decrease in treatment group were more significant than those in control group [NDS score:13.01 ± 1.37 vs.20.63 ± 1.68,CRP (mg/L):8.05 ± 3.97 vs.10.04 ± 4.17,VEGF (ng/L):97.25 ± 13.73 vs.116.43 ± 14.10,TNF-α (ng/L):8.15 ± 2.52 vs.11.54 ± 2.22,all P ＜ 0.05];the total effective rate in observation group was significantly higher than that in control group [92.00％ (46/50) vs.77.55％ (38/49),P ＜ 0.05].The difference of incidence of adverse reaction between observation group and control group was not statistically significant [4.00％ (2/50) vs.8.16％ (4/49),P ＞ 0.05].Conclusions Edaravone combined with β-aescin sodium for treatment of elderly patients with acute cerebral hemorrhage can effectively promote the recovery of neurological function,and its rolemight be related to the regulation of the levels of CRP,TNF-α and VEGF.

Objective To research the effect of the influence of Juantongyin(JTY) on VEGF of endometriotic rats.Methods To observe the effect of high-dose,middle-dose,low-dose JTY and danazol on serum VEGF of endometriotic rats through setting up endometriosis model with rat self-endometria transplantation.Sixty rats were divided into 6 groups randomly:JTY high-dose group(4.29 g/100 g),middle-dose group(1.43 g/100 g),low-dose group(0.48 g/100 g),and danazol group(2.15 mg/100 g),model group and control group 1 mL/100(g?d) of saline.Result There was a marked diversity among model group and other groups(P

Objective To investigate the effects and mechanisms of inositol hexaphosphate on diffe-rentiation of HT-29 human colon carcinoma cell line. Method Cells were exposed to various concentrations (control,1.8 mmol/L,3.3 mmol/L) of IP6 for different time (1d,3d,5d). Its effects on the cells were evaluated by detecting follow indices:(1) Observing ultrastructure of HT-29 cells by transmission electron microscopy. (2) Detecting alkaline phosphate (ALP) activity to evaluate cell differentiation. (3) Expression of c-Myc mRNA was determined by reverse transcription polymerase chain reaction (RT-PCR). (4) Immunocytochemical stain was used to detect expression of Rb protein. Results (1) After having been treated at 3.3mmol/L concentration,the cell structure changed,and appeared tendency of differentiation,such as karyopyknosis,abundant cellular organs and microvillus increase. (2) The activity of alkaline phosphate increased along with the concentration and time. (3) RT-PCR showed that different concentrations of IP6 decreased the expression of c-Myc mRNA. (4) The immunocytochemical stain showed that different concentrations of IP6 increased expression of Rb protein. Conclusion IP6 can induce differentiation of HT-29 cell by augmenting expression of Rb,decreasing expression of c-Myc and blocking cell cycle.

To explore the clinicopathological characteristics of parathyroid crisis.The data of 3 cases from our hospital and 125 cases reported domestically were retrospectively analyzed.All 3 cases were male.And their disease course was 3 months to 10 years.The serum calcium ranged from 3.72 to 5.19 mmol/l.Among 177 retrieved cases,125 had complete basic data.The male/female ratio was 1∶ 1.17,average age (46 ± 14) (15-75) years and average serum calcium (4.30 ± 0.58) (3.75-7.28) mmol/L.There was no difference between genders or benign and malignant pathological types.Among 117 eases with pathological diagnosis,the most common type was adenoma (74.4％).The ratio of parathyroid carcinoma was higher in males than that in females (33.3％ vs.11.7％).Misdiagnosis rate was 25.0％ upon initial presentations.Case fatality rate stood at 9.4％ (17/180) and half of them died preoperatively.