Fatty Liver ; Humans

Objective: To study the chemical constituents in the roots of Aconitum bulleyanum Diels. Methods: The air-dried roots of A. bulleyanum were powdered and extracted by methanol with percolation. After removing the solvent under reduced pressure, the crude extract was dissolved in 1. 5％ HCl solution, and then basified to pH 9 by NaOH (5％) and extracted by ethyl acetate to ob-tain crude alkaloidal extract after ethyl acetate removal. The alkaloidal extract was isolated and purified by column chromatography, and their structures were identified based on spectral analysis ( 1 H-NMR, 13 C-NMR, MS) . Results:Totally 12 diterpenoid alkaloids were isolated from A. bulleyanum and characterized as foresaconitine (1), crassicauline A (2), chasmanine (3), talatisamine (4), 14-debenzoyl-franchetine (5), pengshenine A (6), crassicautine (7), yunaconitine (8), franchetine (9), liljestrandisine (10), transconitine B (11) and pseudoaconine (12). Conclusion:Compounds 3-7, 10-12 are isolated from this plant for the first time.

Objective To study the effect of rosuvastatin(RSVT) on the vascular endothelial function(VEF) ,inflammatory fac‐tors(IF) and prognosis in patients with acute coronary syndrome (ACS) after percutaneous coronary intervention(PCI) .Methods Eighty cases of ACS patients with PCI in our hospital selected from July 2010 to July 2013 were randomly divided into observed group and control group ,40 cases in each group .The observation group were given interference treatment with RSVT while the control group received conventional treatment .The VEF ,IF and prognosis were compared between groups .Results The VWF at 4 weeks after PCI in observation group was lower than that in control group [(92 .6 ± 12 .3)% vs .(105 .4 ± 13 .6)% ,P<0 .05];The ET‐1 at 4 weeks after PCI in observation group was lower than that in control group[(55 .6 ± 5 .6)ng/L vs .(67 .8 ± 7 .4)ng/L ,P<0 .05] .The NO at 4 weeks after PCI in observation group was higher than that of control group[(78 .6 ± 9 .4)μmol/L vs .(63 .2 ± 9 .5)μmol/L ,P<0 .05] .The CRP at 4 weeks after PCI in observation group was lower than that in control group[(5 .4 ± 2 .2) mg/L vs .(10 .5 ± 4 .1)mg/L ,P<0 .05] .The incidence of CVE was 5 .00% (2/40) ,restenosis rate was 2 .50% (1/40) in observa‐tion group ,which was significantly lower than those of the control group [30 .00% (12/40) ,10 .00% (4/40)] ,the differences were statistically significant(P<0 .05) .Conclusion RSVT could effectively improve the VEF and reduce inflammation ,CVE and rest‐enosis rate in patients with ACS after PCI .

Objective: To construct a high effective eukaryotic expre ss ing vector containing mcpr1 gene. Methods: mcpr1 gene w as amplified by PCR from the plasmid T-easy/ mcpr1, then PCR product was in serted into eukaryotic expressing vector pcDNA3.1/V5-His B. The positive recomb inant was identified by PCR analysis, HindIII and BamHI restriction analysis and Sequence analysis. Results: A 400 bp DNA fragment was amplified from the recombinant. Sequence analysis and restriction digest demonstrated tha t the mcpr1 gene was successfully inserted into pcDNA3.1/V5-His B plasmid. Conclusion: The eukaryotic expressed vector pcDNA3.1/V5-His B/ mcpr1 has been successfully reconstructed.

Objective To observe the effect of Jiangzhi Yigan Chongji (JYC) on the nonalcoholic fatty liver tissue PPAR? and Trx mRNA expression,and explore the mechanism of treating fatty liver. Methods The model was made by feeding high-fat diet and the rats were divide into 3 groups:normal group,model group and treated group. Result Expression of liver tissue PPAR? and Trx mRNA in the model group were both decreased. JYC can increase their expression of liver tissue of model rats. Conclusion It is likely to be one of the important mechanisms for JYC in treating nonalcoholic fatty liver.

Objective:The purpose of this study is to locate and characterize splenic trauma with contrast-enhanced ultrasound(CEUS).Methods:A defined mild intraparenchymal lesion was done in 2 pigs.Ultrasound features after trauma were observed by baseline ultrasound,color Doppler flow images(CDFI) and CEUS with a contrast agent(Sono Vue),respectively.Results:In 2 pigs,focal intraparenchymal lesions on spleen with diameters ranging from 10.0 to 10.0 mm could be identified by CDFI and CEUS but not by the baseline ultrasound.Hyperechoic rift in spleen was visible along trauma area as enhancement in splenic parenchyma disappeared on CEUS.Hyperechoic line could be kept in a long time.The hyperechoic rift also appeared at the sub-capsular region beside a traumatic lesion.Hyperechoic rift in traumatic region could be seen even as CEUS was converted to conventional B-mode.Enhancement in the traumatic lesion was clearly seen in CDFI.Conclusion:Hyperechoic rift in spleen was visible along trauma area as a contrast agent is used.CEUS is useful in identifying the grade,range and location of splenic injury.

Traditional Chinese medicine (TCM) diagnostic methods contain inspection, auscultation-olfaction, inter-rogation and palpation. The clinical doctor collects information through the four diagnostic methods. Various symp-toms of the disease are especially used as the main evidence to determine different diseases and to identify the syndrome. Therefore, the TCM four diagnostic methods are the basic means of disease understanding in TCM. This article collected and analyzed clinical data in order to summarize the unique features and focused aspects of TCM four diagnostic methods application on TCM surgery and dermatology. This article was aimed to provide new ideas for TCM four diagnostic methods in the clinical diagnosis of dermatological diseases.

Objective To screen the neonatal malrotation with PITX2 gene exon 2 and 5 gene mutation through the study on molecular genetics.Methods From January 201 2 to December 201 4,1 5 cases of neonatal malro-tation infants(experimental group)and 25 healthy newborn infants(healthy control group)were selected as the research subjects from the First Affiliated Hospital of Shihezi University Medical College.The experimental group included 1 5 ca-ses of volvulus,4 cases of volvulus with duodenal atresia and 3 cases of volvulus with jejunal atresia.The clinical fea-tures were recorded and 3 mL peripheral venous blood from each subject was collected.After ethylenediamine tetraacetic acid (EDTA)anticoagulation,genomic DNA was extracted.Polymerase chain reaction (PCR)was used to amplify the exon 2 and exon 5 of PITX2 gene,and the direct sequencing method was used to screen whether there were mutations in these 2 loci.Results According to the findings of the matching gene,PITX2 gene exon 2 and exon 5 mutations were not detected in 15 cases with intestinal malrotation of the experimental group and 25 healthy newborns in the healthy control group.Conclusions Polymorphisms is not detected in PITX2 gene exon 2 and exon 5 in small groups of newborn,but this does not exclude the possibility the gene caused newborns suffering from intestinal malrotation by other means.

Background:Ulcerative colitis(UC)is a chronic and nonspecific intestinal inflammatory disease and its pathogenic mechanism has not yet been clarified. Intestinal mucosal immune function disorder may play a key role in the pathogenesis of UC. Aims:To investigate the effects of Wumeiwan on expressions of δ-opioid receptor(DOR),β-arrestin1 and Bcl-2 in rats with colitis. Methods:Fifty-six Sprague-Dawley rats were randomly divided into model group,Wumeiwan group, mesalazine group and blank group. Rats in model group,Wumeiwan group and mesalazine group were administered intrarectally with 5% TNBS and 50% ethanol to induce experimental colitis. After colitis models were established,rats in Wumeiwan group and mesalazine group were administered intragastrically with Wumeiwan and mesalazine suspension, respectively,and rats in model group and blank group were given intragastrically with 0. 9% NaCl solution,all for 15 days. On day 16,all the rats were sacrificed and colon samples were obtained. Protein and mRNA expressions of DOR,β-arrestin1 and Bcl-2 in colonic tissue were determined by immunohistochemistry and real-time PCR,respectively. Results:The inflammatory injury in colonic tissue of rats with experimental colitis was significantly attenuated when treated with Wumeiwan,Protein and mRNA expressions of DOR,β-arrestin1 and Bcl-2 in colonic tissue of model group were significantly higher than those of blank group(P < 0. 05). Protein and mRNA expressions of DOR,β-arrestin1 and Bcl-2 in colonic tissue of Wumeiwan group and mesalazine group were significantly lower than those of model group(P < 0. 05), however,no significant differences were found between the two groups(P > 0. 05). Conclusions:DOR-β-arrestin1-Bcl-2 signal transduction pathway may play a central role in the pathogenesis of UC. Intervening this signaling pathway may be one of the mechanisms of attenuating UC by Wumeiwan.

Objective The research aimed to identify symptom clusters of head and neck cancer (HNC) patients.Methods The M.D.Anderson Symptom Inventory-Head & Neck (MDASI-H&N) was applied to measure symptoms of 247 HNC inpatients.Factor analysis was applied to identify symptom clusters.The Spearman correlation analysis was used to find the relationship of symptoms within symptom clusters.Results Five clusters were identified and the Cronbach's α coefficients of symptom clusters were 0.904,0.928,0.898,0.815 and 0.662 respectively.Symptoms within clusters were all significantly interrelated to each other (r=0.393-0.856,P＜0.01).Conclusions Symptoms of HNC patients tend to occur not in isolation,but in symptom clusters.Effective management can not only eliminate or alleviate the symptom burden of HNC patients,but also be cost-effective.