2.A Comparison of some bioactivitiex of A Ⅱ made in China and oversedas
Changling LI ; Xiaoxu LI ; Zhongzhou SHEN ; Janping LIU
Chinese Pharmacological Bulletin 1986;0(04):-
The bioactive identity of A Ⅱ made in China and overseas was demonstrated in different levers including receptor, organ and whole body in this report. Therefore, the A Ⅱ made in China can be used in various pharmacological researches in stead of imports.
3.Ultrasonic study of the right heart function of patients with pulmonary hypertension in ICU
Yuhan ZHU ; Qimin JIANG ; Xiaoxu SHEN ; Nan GUO
The Journal of Practical Medicine 2017;33(2):246-249
Objective To investigate the valuable echocardiographic parameters and to evaluate theright heart function of patients with pulmonary hypertension in ICU. Methods From March 2015 to February 2016,30 patients with pulmonary hypertension in the intensive care unit(ICU) of Dongzhimen hospital were enrolled in the experimental group,and another 33 patients without pulmonary hypertension in ICU were enrolled in the control group. All of the patients were given bedside echocardiography examination. Results Significant differences in the right atrial diameter and area ,the base diameter of the right ventricle ,the intermediate diameter portion ,the distal inner diameter of the proximal end of the right ventricular outflow tract ,the main pulmonary artery diameter ,the Tei index and the E/A were observed between the two groups. However ,no significant differences in the right ventricular free wall thickness,TAPSE,RVFAC,VREF and DT were foundbetween the two groups. Conclusion Tei index,the diameter of the right heart and the main pulmonary artery diameter are proposed to be the promising echocardiographic parameters to evaluate the right heart function of patients with pulmonary hypertension in ICU.
4.Research progress on low carbohydrate diet in the control of overweight and obesity
LU Yanyu ; GUO Huilan ; SHEN Fang ; HUANG Xiaoxu ; ZHANG Pianhong
Journal of Preventive Medicine 2021;33(12):1231-1235
Overweight and obesity are main risk factors for chronic metabolic diseases, and are strongly associated with the increased risk of premature death. Low carbohydrate diet (LCD) has been proven to effectively control body weight and fat mass in overweight and obese patients by short-term (≤6 months) dietary intervention studies. The mechanisms include regulation of energy metabolism, anti-inflammatory, antioxidant, alteration in expression of lipid metabolic-related genes and modulation of intestinal flora. However, the conclusions are inconsistent on whether LCD can cause durable weight loss and reduce the risk of overweight and obesity. This review summarizes the current research progress on the mechanisms, epidemiological studies, intervention studies and potential risks of LCD in controlling overweight and obesity, providing a reference for the future research and clinical application.
5.A Relevant Research on Traditional Chinese Medicine Syndromes with Diastolic Heart Failure
Xiaoxu SHEN ; Sulin MA ; Jun WANG ; Junping LI ; Xian WANG ; Shuoren WANG
Chinese Journal of Information on Traditional Chinese Medicine 2014;(9):40-42
ObjectiveTo investigate the laws of distribution and combination of traditional Chinese medicine (TCM) syndromes of patients with diastolic heart failure (DHF); To research the relevance between TCM syndromes and DHF.Methods A four diagnostic information survey was conducted among 124 DHF patients in order to analyze their basic information and information about TCM four diagnostic methods. Statistical analysis method was used to determine the syndrome types of the DHF patients and the features of syndrome combination.ResultsMost DHF patients were in their sixties or seventies, the number of female patients was larger than that of male patients, and their heart function was in II or III grade. Qi deficiency, phlegm turbidity, Qi stagnation, blood stasis, water stagnation, Yang deficiency and Yin deficiency were the common syndromes. Two-card combination and three-card combination were more common, mainly with Qi deficiency, phlegm turbidity or blood stasis as the main patterns of manifestation. Conclusion This research could provide a clinical basis for the establishment of the standard for the TCM syndromes DHF.
6. Effect of obesity on the treatment of femoral neck fracture with SuperPATH hip arthroplasty
Yu JIANG ; Xiaoxu RONG ; Yimin SHEN
Chinese Journal of Primary Medicine and Pharmacy 2019;26(13):1537-1540
Objective:
To explore the effect of obesity on the treatment for femoral neck fracture in patients with SuperPATH hip replacement.
Methods:
Seventy patients who were treated with SuperPATH hip replacement in the Second People's Hospital of Wuxi from January 2014 to January 2017 were selected in the study.Based on their body mass index(BMI), the patients were divided into obesity group(BMI≥30kg/m2) (23 cases), overweight group(BMI: 25-30kg/m2) (22 cases), and normal group(BMI: 18.5-25kg/m2)(25 cases). The incision length, operation time, blood loss, blood transfusion rate, pain score, hip joint function score, imaging measurement results and postoperative complications were compared among the three groups.
Results:
All 70 patients received the operation successfully.The incision length of the obesity group was (7.4±0.2)cm, which of the overweight group was (7.2±0.1)cm, which of the normal group was (7.1±0.8)cm, the difference among the three groups was statistically significant (
7.Effect of Buyang-Huanwu decoction on cardiac diastolic function in rats with diastolic heart failure
Xin DONG ; Zhen WANG ; Jiebai LI ; Xiaoxu SHEN
International Journal of Traditional Chinese Medicine 2018;40(11):1055-1058
Objective To investigate the effect of Buyang-Huanwu decoction on left ventricular diastolic function in DHF rats.Methods Thirty male Sprague-Dawley rats were randomly divided into the normal group (n=10),the Chinese medicine group (n=6),the western medicine group (n=6) and the model group (n=6).Except for the normal group,the other groups got abdominal aorta stenosis.After 12 weeks,The Chinese medicine group was given Buyang-Huanwu decoction 12.72 g/(kg·d),and the western medicine group was given metoprolo 1 0.004 5 g/(kg·d),and the normal group and the model control group were given water.After once daily for 8 weeks,echocardiography was used to measure EF,E/A ratio and e/a ratio.The heart rate,left ventricular diastolic pressure,left ventricular end-diastolic pressure,tension and maximal change in pressure of left ventricular were measured by Langendorff.Results Compared with the model group,the e/a value (1.11 ± 0.28 vs.0.67 ± 0.20) of the Chinese medicine group significantly increased (P<0.05),and the ventricular muscle tension (0.86 ± 0.64 g vs.1.01 ± 0.33 g) significantly decreased (P<0.05).Conclusions Buyang-Huanwu decoction could improve cardiac relaxation by reducing ventricular muscle tension,thus improving diastolic function of diastolic heart failure rats.
8. Influence of antibiotic concentration on the integration frequency of resistance cassette in the integron
Zehua YANG ; Min HU ; Shuangyan ZHOU ; Jianmin DU ; Bin SHEN ; Yiqing DU ; Xiaoxu LUO ; Zhuanxia LIANG
Chinese Journal of Laboratory Medicine 2018;41(7):531-535
Objective:
To observe the integration frequency of aadA2 resistance cassette at attI site of the integron under different concentration of streptomycin.
Methods:
Class 1 integron with known gene sequence was cloned into plasmid pACYC184 to produce recombinant plasmid pACIDA, meanwhile the integrase gene was cloned into plasmid pET28a to construct recombinant plasmid pETINT. These two recombinant plasmids were consecutively transformed into E. coli BL(DE3). These transformed bacteria was cultured in the LB medium at 37 ℃ overnight with addition of different concentration of streptomycin. The copy number of total integrons and the copy number of integrated aadA2 at attI site of integrons were determined by using real-time PCR. and the integration frequency is the result of the former divided by the latter.
Results:
The resulting frequencies were (1.97±0.24)×10-3, (3.23±1.77)×10-3, (3.27±0.67)×10-3, 0.45±0.13 and 1.32±0.11, with respective streptomycin concentrations of 0, 20, 30, 40 and 50 μg/ml. The background frequency of integration without integrase overexpression was less than (1.75±0.33)×10-7.
Conclusion
These findings indicate that antibiotic concentration significantly increase recombination frequency of aadA2 resistance cassette at attI site of the integron, catalyzed by integron integrase.(
9.Association of JAK/STAT signaling pathway with portal vein thrombosis after splenectomy in cirrhotic patients with portal hypertension
Huan WANG ; Xiaoxu SHEN ; Liangqi CHEN ; Tianlan GONG
Journal of Clinical Hepatology 2022;38(10):2260-2264
Objective To investigate the role of the Janus tyrosine kinase (JAK)- signal transducer and activators of transcription (STAT) signaling pathway in thrombosis formation after splenectomy in cirrhotic patients with portal hypertension. Methods A total of 198 liver cirrhosis patients with portal hypertension who underwent splenectomy and devascularization were recruited from March 2018 to April 2020 and then divided into thrombosis ( n =41) and non-thrombosis groups ( n =157) according to whether portal vein thrombosis occurred 3 months after surgery. mRNA levels of JAK2 and STAT3 in peripheral blood mononuclear cells (PBMCs) were assessed. Comparison of continuous data between groups was performed using t test, while comparison of categorial data between group was performed using chi square test. Clinicopathological data from patients were collected and analyzed for the potential risk factors of portal vein thrombosis by using univariate and multivariate Logistic regression analyses. The prediction values of JAK2 and STAT3 mRNA for portal vein thrombosis were evaluated by area under the receiver operating curve ( AUC ). Results The diameter of portal vein, the diameter of portal vein, the velocity difference of portal vein and the volume of spleen in the thrombus group were significantly higher than those in the non-thrombus group ( t =4.718, 3.945, 8.671, and 2.006 respectively; P < 0.05).The relative mRNA levels of JAK2 and STAT3 in PBMCs of the two groups were significantly increased after surgery ( t =12.933, 15.442, 14.386, 10.896; P < 0.05), and their levels were also much higher in the thrombosis group than in the non-thrombosis group of patients ( P < 0.05). Multivariate Logistic regression analytic data showed that the portal vein diameter, the velocity difference of portal vein and mRNA level of JAK2 and STAT3 were all predictors for thrombosis in these patients ( P < 0.05). The AUC of JAK2 and STAT3 mRNAs was 0.850 and 0.787, respectively in diagnosis of thrombosis in these patients. Conclusion The JAK / STAT signaling was activated in the process of thrombosis after splenectomy in cirrhotic patients with portal hypertension, which may be one of the important mechanisms of postoperative portal vein thrombosis, and may become a potential target for the prevention and treatment of postoperative portal vein thrombosis.