Objective To observe the clinical effect of Oxygen Atomization Inhalation combinated with Tra -ditional Chinese Medicine (TCM) in the treatment of capillary bronchitis .Methods 82 Children with capillary bronchitis were divided into Observation Group (42 cases) and contral group (40 cases).Cases in the Observation Group underwent TCM Agreement medicinal broth and oxygen atomization inhalation , while cases in the contral group underwent Ribavirin atomization .The symptoms of disappear time of signs , clinical effect and adverse circumstance were observed.Results The total efficiency of the observation group was 95.2%, 80% in the contral group.The antifebrile time, disappear time of cough and dyspnea , of the observation group were better than that in the compari -son group.The difference was statistical significant (p <0.05).There was no obvious adverse reactions during the treating periods.Conclusion The clinical effect of Oxygen Atomization Inhalation with Traditional Chinese Medicine (TCM) Agreement Medicinal Broth on capillary bronchitis is confirmed , and the effect of Relieving symptoms , signs quickly and shorten disease time is obviously better than Pure western medicine treatment .In addition, the adverse reactions caused by medicine is also very small ,which is worth promoting for clinical application .

Objective To investigate the difference between two substrains,5-8F and 6-10B,of nasopharyngeal carcinoma cell line SUNE1on expressing metastasis-associated gene 1 (MTA1),and evaluate the silencing effect of lentivirus-mediated RNA interference (RNAi) on MTA1 in human nasopharyngeal carcinoma cell line.Methods The differential expression of MTA1 mRNA and MTA1 protein in 5-8F and 6-10B cell lines were detected by real time PCR and Western blotting respectively.Short interference RNA (siRNA) fragment targeting MTA1 gene was designed using online databases and software.A specific RNAi lentiviral vector targeting human MTA1 gene was constructed and transfected into nasopharyngeal carcinoma cell line 5-8F.Real time PCR and Western blotting were performed to detect the expressive changes in MTA1 mRNA and MTA1 protein in the transfected 5-8F cells respectively.Results Compared with cell line 6-10B,the expressions of MTA1 mRNA and MTA1 protein were higher in 5-8F cell line.Sequence analysis validated the correct insert of siRNA targeting MTA1 gene into the lentiviral vector.Real time PCR and Western blotting results showed that the expressions of MTA1 mRNA and MTA1 protein in 5-8F cell lines were down-regulated significantly after siRNA transfection.Conclusions MTA1 may promote the malignant transformation and enhance the metastatic potential of nasopharyngeal carcinoma cell lines.The expression of MTA1 in 5-8F cells can be inhibited effectively by MTA1-specific siRNA expression lentiviral vector,which provides a valuable tool for investigating the role of MTA1 gene in the carcinogenesis and progression of nasopharyngeal carcinoma.

Objective: To analyze the clinical value of modified endoscopic nipple resection in duodenal papillary adenoma resection Methods: 40 patients with duodenal papillary adenoma were randomly divided into observation group (20 cases) and control group (20 cases)according to the random number table. The patients of control group were treated by conventional resection of papillary adenoma, while the patients of observation group were treated by modified endoscopic nipple resection. The resection rate and the implantation of stent between the two groups were compared. Besides, the occurrence rate of complication and postoperative follow-up between the two groups also were compared. Results: The complete resection rate of observation group (95.00%) was not significant difference with that of control group (90.00%), while en bloc resection rate of observation group (85.00%) significant higher than that of control group (55.00%) (x2=4.28, P<0.05).There was not statistically significant difference between the two groups about the implantation of stent (x2=0.36, P>0.05). In postoperative three months, the difference of recently complication between the two groups was significant (x2=6.14, P<0.05). While the difference of long-term complication between the two groups was no significant (x2=0.36, P>0.05) in more than postoperative three months. After the follow-up of one year, the recurrence rate of observation group (10.34%) was no significant with that of control group (14.29%)(x2=0.17, P>0.05). Conclusion: The modified endoscopic nipple resection can enhance the en bloc resection rate, and reduce the incidence of complications and the recurrence rate. Besides, it has many dominances including higher feasibility in clinical practices. Therefore, its promotion is worthy.

Objective To investigate the risk factors of radiation-induced oral mucositis (ROM) and to provide evidence for the prevention of ROM. Methods The severity of ROM was observed and recorded in 56 patients with nasopharyngeal carcinoma. The association of the severity of mucositis with age , sex, clinical stage, concurrent chemoradiotherapy, white blood cell count,BMI index, smoking, alcohol intake, and history of diabetes were assessed by using multivariate logistic regression analysis. Results The severity of ROM was significantly associated with a decrease in leucocyte count (P<0.05), and it was not markedly correlated with the other eight factors (P>0.05). Conclusions Leukopenia was one of the risk factors for the development of ROM. In clinical pratice,leukopenia should be corrected in patients with bone marrow depression due to radiotherapy in a timely manner.

Objective To investigate whether monocyte chemoattractant protein-1 mediates lupus nephritis (LN) in BXSB mice and whether methylprednisolone ameliorates LN by inhibiting MCP-1 expression. Methods 18-week-old male BXSB mice (n=6) displaying clinical symptoms of glomerulonephritis were treated (i.p.) for 3 weeks with MPS (25 mg?kg-1?d-1) dissolved in N.S. BXSB controls were age-and sex-matched BXSB mice (n=6) that received N.S. alone. Age- and sex-matched BALB/C mice (n=6) were used as normal controls that were treated in the same way as the BXSB controls. MCP-1 expression was investigated by RT-PCR and immunohistochemical examination. The heaviness of proteinuria was also evaluated. Medical imaging analysis was performed to detect the relationship between MCP-1 expression and proteinuria. Results MCP-1 was strongly expressed in kidneys of all BXSB mice, stronger staining was found in cytoplasm of tubular epithelial cells than glomerular, while no expression was found in kidneys of BALB/C mice. MCP-1 expression in tubular cells in BXSB control group was closely correlated with proteinuria. MPS significantly reduced proteinuria and MCP-1 expression and down-regulation of MCP-1 expression in tubular cells was also closely correlated with that of proteinuria. Conclusion MCP-1 over-expression may mediate LN in BXSB. MPS ameliorates LN partially by inhibiting MCP-1 expression.

Objective To study the route of intrauterine infection of chlamydia trachomatis (CT) Methods Seven hundred and seventy two cervical samples from in women and 105 matched maternal labom neonatal samples composed of cervical samples,cord blood, amniotic fluid, conjunctival and nasopharyngeal samples of neonate were detected by PCR SSCP and DNA sequencing technique Results CT were detected in 87 of 772 (11 3%) cervical samples In the 81 matched maternal infant samples from pregnant women with cervical CT positive, CT were not detected in all of the cord blood samples In the 30 CT positive neonatal samples, 26 were from cases of vaginal delivery and 4 from cases of caesarean section Statistical analysis showed a significant difference between the groups of caesarean section and the vaginal delivery ( P

Objective To investigate the possible roles of Langerhans cells(LCs) and plasmacytoid dendritic cells(pDCs)in the pathogenesis of condyloma acuminatum(CA). Methods Tissue specimens were obtained from the lesions of 23 patients with CA and from perilesional normal skin of 13 of the 23 patients. The expressions and distribution of CD1a, CD2AP and CD123 were analyzed using the streptavidin-peroxidase (SP) immunohistochemical method. Statistical analysis was carried out to compare the density and percentage of CD1a + LCs, CD2AP + pDCs and CD123 +pDCs between lesional skin and perilesional normal skin. Results Most CD1a + LCs were observed in the prickle cell layer of the epidermis, and a few in the papillary layer of the dermis. There was no significant difference in the density or percentage of CD1a+ LCs in the epidermis or dermis between the lesional and perilesional skin specimens (all P > 0.05). CD2AP + pDCs and CD123 + pDCs were distributed mainly in the papillary layer of the dermis, and their density and percentage were significantly higher in the lesional than in the perilesional skin specimens (all P < 0.05). Conclusion Human papillomavirus infection may affect the quantity of pDCs but not LCs in local mucous membranes.

Objective To study the effects of 4 1BBL on antitumor immunity induced in vivo by murine 4 1BBL gene transfected hepatocellular carcinoma cell line Hepa1 6. Methods Eukaryotic expression vector pcDNA3 1(+) m4 1BBL was transfered into murine hepatocellular carcinoma cell line Hepa1 6 via lipofectamine method. The transfected cells were selected in RPMI1640 containing G418(400～800 ?g/ml) and termed as Hepa1 6 m4 1BBL.The TCV m4 1BBL was obtained by treating with mitomycin (MMC). Three models (immunological model, early model, and later model) were established for the study of anticancer effects of TCV m4 1BBL. Results The syngeneic mice were completely protected and could survive free from tumor for a long period by inoculation with TCV 4 1BBL. In early models, TCV 4 1BBL showed strong immunotherapy effects( P 0 05). Conclusion The antitumor effect against syngeneic murine hepatocellular carcinoma cell line in vivo is significantly enhanced by the treatment with TCV 4-1BBL.

Objective To observe the effects of Okinawa Habu apoxin protein-1 (OHAP-1) on the proliferation inhibition of rat C6 glioma cells and its mechanisms. Methods MTT colorimetric analysis was used to measure the inhibitory effect of OHAP-1 with different doses(2.5,5.0,and 10.0 mg?L-1) on C6 glioma cells .RT-PCR was used to evaluate the mRNA expressions of bcl-2 and bax genes.The activity of superoxide dismutase (SOD) and the level of maleicdialdehyde (MDA) in the C6 glioma cells were also examined. Results The proliferation of C6 glioma cells was significantly inhibited by different doses of OHAP-1(2.5,5.0,and 10.0 mg?L-1).The inhibitory rate were 49.77%,67.65%,and 76.42%,respectively.The inhibitory rate in 2.5,5.0, and 10.0 mg?L-1 groups were higher than that in control group(P

Objective To establish a suitable extraction and purification process line for industrial production of liquiritin. Methods With the extraction rate of liquiritin as index, orthogonal test was used to determine the optimum conditions; with the retention rate of liquiritin and impurity removal rate as the indexes, orthogonal test was used to optimize the best ultrafiltration process parameters. Results The optimum extraction conditions were: 24 times 0.75%ammonia water, extracted three times, each time under 60 min. The liquiritin average extraction rate was 72.3%. The best ultrafiltration process parameters were: 10 nm inorganic ceramic membrane, pressure of 0.12 MPa, temperature of 25 ℃. The liquiritin average retention rate was 98.9%, and the average removal rate of impurity was 23.3%. Conclusion This process has low production cost and good safety, and is suitable for industrial application.