Objective To study the relation between TCM syndromes of systemic lupus erythematosus (SLE) and expression of Fas and sFas. Methods The relation between the TCM syndrome types of SLE patients and experssions of Fas and sFas was analyzed through detecing Fas and sFas in the blood of SLE patients and control group. Results The expression of Fas in heat-toxin syndrome and Yang deficiency syndrome of spleen and kidney were higher than control group and interior heat syndrome due to Yin deficiency. Serum sFas of SLE patients was higher than control, but there was no significant difference among the three syndrome types. Conclusion The expression of Fas and sFas has obvious relation with TCM syndrome types and can be used for the syndrome differentiation of SLE.

Objective:To study on effect of Danshao Huaxian Capsule on proliferation of activated hepatic stellate cells(HSCs)in vivo and in vitro.Methods:(1)In vitro experiment:57 male Wistar rats were divided into a normal group,a model group and a treatment group.The rats of model group and treatment group were made hepatic fibrosis model with complex stimulation of CCl_4,drinking alcohol,high lipids and low protein diet for 8 weeks.The treatment group were treated for 8 weeks with intragastric perfusion of Danshao Huaxian Capsule,1g/kg.At the end of experiment,a part of the rats were used for detection of hepatic functions and hepatic fibrosis degrees,and another part of the rats were used to separate HSCs.Cell cycle percentage was detected with flow cytometry.(2)In vivo experiment:prepare Danshao Huaxian Capsule serum of the rat;separate cultured HSCs of the rat and divide into calf serum group,normal rat serum group and Danshao Huaxian Capsule serum group.5%,10% and 20% of the above serum was respectively added into the cultured HSCs.Primary HSCs proliferation was detected with MTT method.Results:(1)In vitro experiment:The hepatic function,hepatic fibrosis level and HSCs proliferation reduced significantly.(2)In vivo experiment: Danshao Huaxian Capstde serum group could significantly inhibit proliferation of HSCs as compared with calf serum group and normal rat serum group at the same content.The action showed dose-dependence.Conclusion:Danshao Huaxian Capsule can obviously inhibit proliferation of HSCs in vivo and in vitro.

Confocal microscope and flow cytometry were used to study the adhesion and the expression of adhesive molecules between different metastatic lung cancer cells and endothelial cells (EC) in vitro. Tea polyphenols (TP) were tested for their effect on tumor cell endothelial adhesion.The results showed that the adhesion and expression of CD44?CD54 were significantly higher in PG cells than those in D9 cells. Treatment of EC monolayers with LPS(0 5ug/ml) for six hours caused an increase in adhesion of PG to EC and adhesive molecules expression. TP could inhibit the adhesion of PG to EC and expressions of CD44 and CD54 in dose dependent manner. It suggested that adhesion molecules might play an important role in mediating the adhesion of lung carcinoma to EC and hematogenous metastasis; TP had an inhibitory effect on metastasis of lung cancer.

Objective Implement random sample from a simulation population,to evaluate the The impact of samplesize and sample-process on several usual importance evaluate methods,observe the stability of those methods.Methods This study introduced existed importance methods,using PROC SURVEYSELECT procedure to sample a fixed population for 1000 times,generating 1000 same size sample,to evaluate the stability of relative importance methods.We sampled the population to generate datasets with different sample size to observe impact of sample-size on those methods.Results The sum of squared correlation coefficients' estimator is bigger than model R-square,squared standardized regression coefficients' sum is smaller.In contrary,sum of the Product Measure,Relative Weight and Dominance Analysis are extremely close to model R-square.When the sample size small than 1000,the estimator have obviously variation,but the variation decreased when the sample size rise up.Conclusion The dominance analysis has best stability,also has the best match of model R2 in those methods.

Objective To control the quality of Gongyankang granule.Methods Paconiflorin in Gongyankang granule was determined by HPLC,using a Phenomenex Prodigy Thermouest(250×4.6 mm 5μm BDS C18).Mobile phase was consisted of CH_2OH-H_2O-H_3PO_4(30:70:0.1)with the UV detector set at 230 nm.Results The paeoniflorin showed linear correlation(r=0.9998)within the range of 0.01026～0.06156mg.The average recovery rate was 99.12%with RSD of 1.61%.Conclusion This method was simple,accurate and with good reproducibility,thus it can be used for quality control of Gongyankang Granule.

Objective To study the effectiveness of interventional chemotherapy in combination with embolization for bulky cervical carcinoma in stage Ⅱb.Methods Cases divided into preoperative interventional group(A)and preoperative radiotherapy group(B).Radical surgery for uterine and pelvic lymph node was performed in each group 2～ 4 weeks after treatment.Results The 3 year survival rates were 91.0% and 88.48%,and 5 year survival rates were 81.79% and 73.37% in A and B,respectively.The recurrence rates were 21.7% in A and 48.6% in B(P

In 2012, the contracted service with the general practitioners ( rural doctors) was implemented in Zhejiang Province. This paper conducted an analysis on the existing problems in the contracted service from three as-pects of policy background, policy objective, main measures and the following suggestions have been put forward:Strengthening the whole family team of doctors and information construction, and improving the multi sectoral coopera-tion mechanism in order to establish the classified diagnosis and treatment system as targeted to lay a good foundation.

Objective To explore the clinical curative effect of irinotecan HCL trihydrate combination with cisplatin in treatment of non.small-cell carcinoma(NSCC)by observing the clinical curative effect,adverse reactions and the improvement of the quality of life post therapy.Methods 120 patients with NSCC were divided into two groups.60 cases in control group were treated with cisplatin and topotecan while ifinotecan HCL tfihydrate combination with cisplatin chemotherapy for 60 case of patients in observation group.The curative effect and Kamtfsky point were observed.Results The effective rate of observation group was 56.7%,which was significantly higher than that of the control group(41.7%),and differences between the two groupswas statistically significant(P＜0.05).Average survival time for treatment group was(9.3±4.2)months that was longer than that of the control group[(7.2±3.2)months](P＜0.05).The result of Kamofsky classification standard after chemotherapy also indicated the same situation(P＜0.05).Conclusion Irinotecan HCL trihydrate combining with cisplatin in treatment of non-small cell lung cancer had a effective therapy result and could reduce the toxicity.as well as improving the quality of life of the patients.

Objective To clarify the effect of myofunctional treatment on primary and mixed dentition malocclusion.Methods Twenty-seven children were selected,3 were in primary dentition among which 2 showed deep overbite.24 were in mixed dentition and presented crowding in anterior area among which 4 children combined with anterior crossbite,2 showed deep overbite and deep overjet.All the patients were treated with myofunctional trainer such as INFANT,T4K or I2N appliances according to their age and malocclusion type.The patients were instructed to wear the trainers for at least one hour a day keeping their lips together or overnight while sleeping.Muscle exercises were done when wearing the trainer during daytime.Results After 6-10 months,24 patients who were in good compliance had their teeth aligned,deep overjet and deep overbite corrected,and facial profile improved.Conclusions Myofunctional treatment is effective on primary and mixed dentition malocclusion.It is important to have patients' good compliance.