Objective To investigate the role of total bile acid (TBA) in pathogenesis of essential hypertension.Methods The concentration of serum TBA was measured by enzymatic method in 127 cases including 30 healthy controls,21 secondary hypertensive patients and 76 essential hypertensive patients.Results There was a significant increase of TBA in essential hypertensive group compared with that of control group and secondary hypertension (P 0.05) .In essential hypertensive group,the levels of TBA in grade 3 hypertension were higher than that of grade 1 hypertension (P0.05).Conclusion The abnormal increasing of TBA may play a role in pathogenesis of essential hypertension.

OBJECTIVE:To facilitate Chinese pharmaceutical industry's understanding and application of the new "Patent Law".METHODS:The amendment of the "Patent Law" related to 3 aspects of medicine,i.e.specifying regulations on the information disclosure in the utilization of genetic resources,adding the clause of forced permission for the patent drugs for the purpose of public health,and introducing the Bolar exception clause was analyzed using law hermeneutic.RESULTS & CONCLUSIONS:The medicine-related amendment of the new "Patent Law" generally reflects an international tendency of restricting pharmaceutical patents so as to maintain public interests,which is in line with the status quo of low technological level of pharmaceutical industry in China.

Objective To discuss and analyze clinical pathological characteristics of senile recurrent ovarian cancer and its influences on prognosis.Methods 140 senile ovarian cancer patients who have been admitted in ourhospital were selected as study subjects.103 cases with recurrent ovarian cancer were selected as observation group (group A),and the rest 37 cases who did not suffer from recurrent ovarian cancer were selected as control group(group B).Pathological characteristics of carcinoma tissues after their first admission in our hospital were compared between the two groups.And pathological characteristics of carcinoma tissues of group A after their second admissionin our hospital were analyzed,comprehensively analyzed prognosis situations and clinical pathological characteristics of patients in these two groups.Results In group A,the number of poorly differentiated carcinoma tissues was obviouslymore than group B,and the number of patients whose neoplasm staging was M stage was more than that in group B,the differences between the two groups were significant (x2 =13.48,15.87,all P ＜ 0.05).The incidence rate of adverse reactions between the two groups also had statistical significance(x2 =13.8,11.6,14.5,13.9,14.9,13.4,all P ＜0.05).Conclusion The clinical pathological characteristics of senile recurrent ovarian cancer can reflect patients'prognosis situations in some degree,and has certain clinical value in evaluation of patients' disease recurrence and prognosis.

Objective To investigate the personality patterns, coping styles and social support statue of the patients with stroke.Methods 68 patients of stroke and 68 normal controls were measured with Eysenck Personality Questionnaire (EPQ)、Coping Style Questionnaire (CSQ) and Social Support Scale (SSS).Results The scores of E and N(11.98?4.79,12.98?4.73) were very higher in stroke group than those of in normal control group(9.46?4.18,9.60?4.70)(all P

Objective To investigate whether monocyte chemoattractant protein-1 mediates lupus nephritis (LN) in BXSB mice and whether methylprednisolone ameliorates LN by inhibiting MCP-1 expression. Methods 18-week-old male BXSB mice (n=6) displaying clinical symptoms of glomerulonephritis were treated (i.p.) for 3 weeks with MPS (25 mg?kg-1?d-1) dissolved in N.S. BXSB controls were age-and sex-matched BXSB mice (n=6) that received N.S. alone. Age- and sex-matched BALB/C mice (n=6) were used as normal controls that were treated in the same way as the BXSB controls. MCP-1 expression was investigated by RT-PCR and immunohistochemical examination. The heaviness of proteinuria was also evaluated. Medical imaging analysis was performed to detect the relationship between MCP-1 expression and proteinuria. Results MCP-1 was strongly expressed in kidneys of all BXSB mice, stronger staining was found in cytoplasm of tubular epithelial cells than glomerular, while no expression was found in kidneys of BALB/C mice. MCP-1 expression in tubular cells in BXSB control group was closely correlated with proteinuria. MPS significantly reduced proteinuria and MCP-1 expression and down-regulation of MCP-1 expression in tubular cells was also closely correlated with that of proteinuria. Conclusion MCP-1 over-expression may mediate LN in BXSB. MPS ameliorates LN partially by inhibiting MCP-1 expression.

Objective To observe the clinical efficacy of traditional Chinese medicine retention-enema in the treatment of ulcerative colitis. Methods 116 cases with ulcerative colitis were randomly divided into treatment group and control group, with58 cases in each group. The treatment group adopted the therapy of traditional Chinese medicine retention-enema after cleaning up the bowel, while the control group adopted the therapy of conventional Western Medicine retention-enema. Both of the two groups were treated for one course. Results The total effective rate of was 87% in the treatment group and 42.5% in the control group. The difference between the two groups was obvious (P＜0.05), with the treatment group being better than the control group. The retention time of medicine in intestinal tract was obviously prolonged in the treatment group. Conclusion The clinical efficacy of tradidonal Chinese medicine retention-enema treatment is obviously better than conventional Western medicine retention-enema treatment.

Objective:To observe changes of serum levels of myeloperoxidase (MPO) and lipoxin A4 (LXA4) in pa‐tients with coronary heart disease (CHD) and explore its clinical significance .Methods :A total of 120 CHD patients treated in our hospital from Jun 2013 to Sep 2014 were selected as CHD group ,another 40 healthy subjects were re‐garded as normal control group .According to CHD type ,patients were divided into stable angina pectoris (SAP) group (n=36) ,unstable angina pectoris (UAP) group (n=46) and myocardial infarction (MI) group (n=38) .Ac‐cording to plaque nature assessed by CT value ,patients were divided into calcified plaque group (n= 27) ,mixed plaque group (n=31) and non-calcified plaque group (n=62) .Levels of MPO and LXA4 and ratio of MPO/LXA4 were compared among all groups .Results:Compared with normal control group ,there were significant rise in MPO level [ (167.2 ± 20.4) U/L vs .(218.3 ± 32.5) U/L] and MPO/LXA4 [ (0.78 ± 0.08) vs .(1.34 ± 0.27)] ,and sig‐nificant reduction in LXA4 level [ (214.6 ± 31.3) nmol/L vs .(162.4 ± 22.4) nmol/L] in CHD group ,P<0.05 or<0.01 .Compared with SAP group ,there were significant rise in MPO level [ (180.4 ± 21.6) U/L vs .(230.3 ± 32.5) U/L vs .(238.6 ± 44.7) U/L] and MPO/LXA4 [ (0.97 ± 0.11) vs .(1.37 ± 0.23) vs .(1.62 ± 0.25)] ,and significant reduction in LXA4 level [ (184.7 ± 23.7) nmol/L vs .(156.3 ± 21.2) nmol/L vs .(148.4 ± 19.6) nmol/L] in UAP group and MI group ,and MPO/LXA4 of MI group was significantly higher than that of UAP group , P<0.05 or < 0.01 . Compared with calcified plaque group and mixed plaque group , there were significant rise in MPO level [(196.3 ± 27.2) U/L vs .(211.2 ± 24.6) U/L vs .(231.6 ± 26.5) U/L] and MPO/LXA4 [(1.13 ± 0.14) vs .(1.26 ± 0.16) vs .(1.51 ± 0.21)] ,and significant reduction in LXA4 level [ (174.3 ± 23.4) nmol/L vs .(167.4 ± 21.2) nmol/L vs .(154.6 ± 19.2) nmol/L] in calcified plaque group ,and MPO/LXA4 of mixed plaque group was significantly higher than that of calcified plaque group , P<0.05 or <0.01 .Conclusion:There exist significant ab‐normal levels of MPO and LXA4 in CHD patients ,ratio of MPO/LXA4 is more helpful for determining disease se‐verity and stability of atherosclerotic plaque .

Constructing internal quality control system is an important measure to improve teaching quality in the universities.Teaching supervision plays an important role in the teaching quality guaranteeing system.This article introduced and summarized the practice of teaching supervision in Tianjin Medical University including the establishment of three-leveled teaching supervision,emphasis of process management,innovation,significance and achievements of teaching supervision.

Objective:To analyze the mutation of 5′ noncoding region of BCL 6 gene in extranodal diffuse large B cell lymphoma (DLBCL) and the relationship between mutation and clinical characteristics.Methods:Extract DNA from paraffin bedded extranodal DLBCL cases,then PCR and sequenced.Meanwhile retrospective analysis was done.Results:The mutation rate was 13 64%(6/44).The clinical characteristics,such as age、sex、B symptom、bulky tumor mass、stage、the level of LDH、BM involvement and CR,had no significant influence on BCL 6 mutation;the survive time of DLBCL with/without BCL 6 mutation had no obvious difference,but there was a tendency the survival time of mutated patients was longer than that of non mutated (the mean survival time was 74 and 65 02 months,respectively);mutation wasn't an independent prognostic factor.Conclusion:The mutation of 5′ noncoding region of BCL 6 gene maybe partly involves in the mechanism of extranodal DLBCL. [

Objective To observe the effect of chronic variable stress on the forced swimming test of rats,and investigate its preliminary mechanism.Methods First a mark was given to each Wistar rat by forced swimming test.12 Wistar rats which had similar scores were selected and divided into control group and model group averagely.The behavior changes of rats were induced in the forced swimming test by chronic stress and the plasma cortisol level was analyzed through radioimmunoassay(RIA) methods.The changes of hippocampus CA3 structure were observed with microscope.Results Compared with control group,chronic variable stress increased immobility time,reduced climbing time evidently and enhanced the weight of adrenal gland and the level of plasma cortisol(P