Objective To retrospectively review the clinical data of acute oral paraquat (PQ) poisoning patients and the relationship of early serum creatinine leveles with respiratory failure , to predict and guide the clinical diagnosis and treatment .Methods Seventy-two PQ poisoning patients from the 2008 to 2012 , according to the occurrence of respiratory failure were divided into two groups , group A:patients with respiratory failure (28 cases);and group B:patients without respiratory failure(44 cases).The first serum creatinine detection early after poisoning as the basic value and the highest value within 48 hours, and the difference between the two ratios was compared .Then the sensitivity, specificity, accuracy, Youden index, receiver operating characteristic(ROC) area under the curve(AUC) were also calculated, and the ROC AUC was drawn.Results The creatinine basic value ,the highest valve and their ratio were obtained , and the difference was statistically significant .The best predictor boarder of creatinine basic value was 53 μmol/L, with forecasting moderate effect,and that of the highest value ,their ratio and the creatinine difference value was 183μmol/L, 3.39 and 113μmol/L, respectively.Conclusion The higher the early serum creatinine level rises , the higher the risk of respiratory failure increa-ses after PQ poisoning .The basic value , the highest value within 48 hours and their difference and ratio could predict the possibility of respiratory failure after PQ intoxication .

OBJECTIVE:To study the effect of erythromycin on the voltage-dependent K~+ current in hippocampal neurons of mice.METHODS:Isolation of mice's hippocammal neurons cells and whole-cell patch-clamp techniques were applied to study the change of voltage-dependent K~+ current of hippocampal neurons and the current-voltage curves in mice before and after treatment with erythromycin.RESULTS:Erythromycin inhibited the voltage-dependent K~+ current of mice's hippocampal neurons,and it down-regulated the peak current from(810.67?250.86)pA to(529.96?171.83)pA(P

Objective To study the effective anticoagulant components of the fermented products of mycelium from Tremella aurantialba 9800. Method To adopt system menstruum deal with fermented products of mycelium from Tremella aurantialba,then mycelium from tremella aurantialba was studied with the clotting time of the thrombogen,the whole blood clotting time in mice and thrombosis experiment in mice. Result Butanol extract of fermented products of mycelium from Tremella aurantialba behave anticoagulant function. Ethyl acetate extract and water extract also behave feeble activity. Conclusion The effective anticoagulant components of the fermented products of mycelium from Tremella aurantialb is butanol extract.

Objective To investigate the prognostic factots by analyzing clinicopathologlcal characteristics of 972 cases of colonic carcinoma. Methods Clinical and pathological data of 972 patients with colonic carcinoma treated surgically were studied by univariate and multivariate analysis,survival rate was calculated by life-table method and compared by Log-rank test. Results The univariate analysis indicated that the predictors of survival were age,perioperative blood transfusion, perioperative carcinoembryonic antigen(CEA)level,tumor gross type,depth of tumor invasion,lymphatic invasion,liver metastasis,extrahepatic metastasis,local recurrence、peritoneal seeding,pathological type,TNM stage and type of lymph node clearance.The multivariate analysis indicated that the independent predictors of suivival were age,periopemtive CEA level,tumor gross type,lymphatic invasion,liver metastasm,extrahepatic metastasis,local recurrence、peritioneal seeding,pathological type,type of lymph node clearance and TNM stage. Confclusions Lymphatic invasion is the most important independent prognostic factor of patients with colonic carcinoma treated surgically.

Objective To detect the expression of T cell immunogloblin and mucin domain-containing molecule 3 (Tim-3) mRNA in peripheral blood mononuclear cells (PBMC) isolated from asthmatic children and asthmatic murine,and to explore the role of Tim-3 in bronchial asthma.Methods Recruiting 97 cases asthmatic children,47 children with acute asthma exacerbation(acute attack group),50 children with asthma remission (remittent group)and 42 children as the healthy control group.The Tim-3 mRNA expression was measured by using reverse transcription-polymerase chain reaction(RT-PCR) method.The levels of interleukin-6 (IL-6) and transforming growth factor-5 (TGF-β)were determined with enzyme-linked immunosorbent assay (ELISA),and to analyze the correlation between Tim-3 mRNA and the level of IL-6.The asthmatic murine models were randomly divided into 2 groups:normal control group and asthma group.There were 10 mice in each group.PBMC in each group were collected.Expression of Tim-3 mRNA was detected by using real-time PCR.The level of CD4 + IL-17 + and CD4 + CD~ + FoxP3 + which reflected Th17 and regulatory T cells(Treg) expression respectively were detected by using flow cytometry,and the correlation between Tim-3 and Thl7/Treg level was analyzed.Results Expression of Tim-3 mRNA in children with acute asthma exacerbation was significantly higher than those in remission stage and healthy control group (all P ＜ 0.05).The expression of Tim-3 mRNA in children with asthma remission was significantly higher than that in healthy control group (P ＜ 0.05),and the level of IL-6 in children with acute asthma exacerbation was significantly higher than those in remission stage and healthy control group (all P ＜ 0.05).In the murine model of asthma group,the level of Tim-3 mRNA in PBMC was significantly higher than that in normal control group (P ＜ 0.01),and in asthma group CD4 + IL-17 + expression was significantly higher than normal control group but CD4 + CD25 + FoxP3 + was significantly lower than that in normal control group (all P ＜ 0.05),and the ratio of Th17/Treg increased in asthma group compared with normal control group (P ＜ 0.05).Tim-3 mRNA expression in PBMC was correlated positively with CD4 + IL-17 + level and Th17/Treg ratio respectively (all P ＜ 0.05),but was correlated negatively with CD4 + CD25 + FoxP3 + level(all P ＜ 0.05).Conclusions The high level expression of Tim-3 mRNA in asthmatic children participated in the airway inflammation,which is associated with IL-6,and animal experiments indicating that Tim-3 is closely related with the imbalance of Th1 7/Treg,indicated that Tim-3 may play a certain role in asthma.

Information Dissymmetry is a common phenomenon in this economical market.None of us can master all information about anything.The relative difference of the information's occupancy leads to a sharp distinction of the bargainer' status currently.During the service of our hospital library,Information Dissymmetry seriously restricts the development of our library and makes many readers dissatisfied.The author analyzed the problem in progress of service to the reader and finally put forward the countermeasure of this progress reconstruction in the hospital library.

Objective To explore the effect of execution sheet for health education in chronic heart failure (CHF) patients. Methods About 50 CHF patients hospitalized from October 2015 to December 2015 were assigned as the control group and another 50 CHF patients hospitalized from January to March 2016 were set up as the experiment groups. The control group was instructed by the regular health education and the experiment group was instructed by the execution sheet for health education. Results The executive ability in the experiment group were stronger than that of control group (P<0.01). The level of self management among the patients in the experiment group was higher than those of the that control group. Conclusions The health education execution sheet increase the patients' self-management and strengthen nursing ability for discharged patients. It is worthy of popularization in clinical practice.

Objective:To analyze the mutation of 5′ noncoding region of BCL 6 gene in extranodal diffuse large B cell lymphoma (DLBCL) and the relationship between mutation and clinical characteristics.Methods:Extract DNA from paraffin bedded extranodal DLBCL cases,then PCR and sequenced.Meanwhile retrospective analysis was done.Results:The mutation rate was 13 64%(6/44).The clinical characteristics,such as age、sex、B symptom、bulky tumor mass、stage、the level of LDH、BM involvement and CR,had no significant influence on BCL 6 mutation;the survive time of DLBCL with/without BCL 6 mutation had no obvious difference,but there was a tendency the survival time of mutated patients was longer than that of non mutated (the mean survival time was 74 and 65 02 months,respectively);mutation wasn't an independent prognostic factor.Conclusion:The mutation of 5′ noncoding region of BCL 6 gene maybe partly involves in the mechanism of extranodal DLBCL. [

Objective To investigate the effect of tumor necrosis factor-α(TNF-α)on cytokines expression in mice with different tidal volume(VT). Methods Thirty six SPF male BALB/c mice were randomly divided into three groups:A group(spontaneously breathed 4 h),S and L group(mechanically ventilated 4 h,VT=6 mL/kg and VT=20 mL/kg). The three groups were then randomly divided into two subgroups(C and T group,n=6). Mice in C group were injected with NS,while that of T groups were injected with TNF-α at 20 μg/kg from tail intravenous. Morphological manifestation was observed by hematoxylin and eosin staining. Ultrastructure of macrophages were examined by transmission electron microscopy. The expression of TNF-α,interleukin(IL)-1β,IL-6,IL-10 in serum and BALF were measured by ELISA. The expression of NF-κB mRNA and protein were detected by RT-PCR and WB,respectively. Results Compared with A and S group,following could be observed under light microscope in L group:the pulmonary interstitial edema,consolidation of part of the lung tissue,alveolar space fusion and a number of inflammatory cells infiltration. The expression of TNF-α,IL-1β,IL-6,IL-10 in serum and BALF,the expression of NF-κB mRNA and protein in macrophages of L group were significantly higher than those of A and S group(both P<0.05). The expression of these cytokines and NF-κB mRNA and protein were increased in T group (P < 0.05). The expression NF-κB is positively correlated with the expression of TNF-α,IL-1β and IL-6. Conclusion TNF-α is a key factor in inflammation of ventilation induced lung injury,which may provide a new method for prevention of VILI in clinical practice.

Objective To evaluate the therapeutic effects and prognostic factors of transanal local excision of rectal carcinoma. Methods We retrospectively analyzed 116 cases who underwent transanal lical excision for rectal carcinoma from 1995 to 2008 with the mented of Cox Regression analysis.Result The survival time of all the patients were from 14 to 160.5 months.median time was 58.5 months.Five-year overall survival rate was 72%.ten-year overall survival rate was 53%.There were 16 cases of local recurrence with a rate of 13.8%.In the univariate survival analysis:histopathology,depth of tumor invasion,radiotherapy,recurrence and metastases were the predictors of survival.In the Cox regression analysis:depth of invasion,recurrence and metastases were the independent prognostic taetors for surwval.For T_1 stage,its overall local recurrence rate was 6.3%,five-year overall survival rate was 93%,ten-year overall survival rate Was 85%:For T_2 stage,its overall local recurrence rate was 14.8%,five-year overallsurvival rate Was 63%.ten.year overall survival rate was 45%.For T_1 stage,there Was no local recurrence with radiotherapy:For T_2 stage,local recurrence rate Was 14.6%.From the surivival curve,there Was no difference betwecn the patient accepted radiotherapy or not(T_1:P=0.260,T_2,P=0.262).But for local recurrence,the differences Was significant(P=0.002). Conclusion The result of transanal local excision of rectal carcinoma is satisfactory with T_1 stage,but it is not suitable for T_2 stage tumors.