Objective To explore the clinical effect of hysteroscopic resection in the treatment of submucous myoma.Methods The clinical data of 78 submucosal myoma patients with hysteroscopic resection which were diagnosed by B ultrasound and hysteroscopy were retrospectively analyzed,including 35 cases of type O,23 cases of type Ⅰ,20 cases of type Ⅱ.Results Application of hysteroscopic resection in the treatment of type O and type Ⅰ submucosal myoma,the rate of postoperative satisfaction was 100.0％.There were 3 cases of multiple myoma in 20 cases of type Ⅱ,which were not one time of resection,and were taken the second times of resection 4 months after operation.In all operation,there was on complications,and the rate of postoperative satisfaction was 96.2％.Conclusion Hysteroscopic resection in the treatment of submucous myoma for the preservation of reproductive function and not affecting ovarian function has the advantage of less bleeding,short operation time,rapid postoperative recovery,shorter hospitalization time,and greatly improve the quality of life of patients,and has good clinical application value,it provides the effective treatment for clinical,and is worthy of clinical popularization.

BACKGROUND:Dental pulp stem cel s are characterized by multi-lineage differentiation and proliferation abilities and are easy to obtain, so they are becoming an issue of concern in regenerative medicine. OBJECTIVE:To provide clues and direction for further study by analyzing progress of domestic and overseas research on dental pulp stem cel s, and summarizing their application in regenerative medicine. METHODS:The“dental pulp stem cel , regenerative medicine, tissue engineering”in Chinese and English served as the search terms to search articles related to dental pulp stem cel s and regenerative medicine, published from 2000 to 2015 in Medline, PubMed, CNKI, Wanfang and Cqvip databases. Total y 46 articles were selected for overview. RESULTS AND CONCLUSION:Dental pulp stem cel s, which hold the capacity of self-renewal and multi-lineage differentiation, are relatively easy to obtain, and exhibit a great potential in regenerative medicine. The research of dental pulp stem cel s in repairing bone defects has entered the clinical trial phase, but the research of cel differentiation into other tissues is stil in basic trial phase and needs further development.

OBJECTIVE:To facilitate Chinese pharmaceutical industry's understanding and application of the new "Patent Law".METHODS:The amendment of the "Patent Law" related to 3 aspects of medicine,i.e.specifying regulations on the information disclosure in the utilization of genetic resources,adding the clause of forced permission for the patent drugs for the purpose of public health,and introducing the Bolar exception clause was analyzed using law hermeneutic.RESULTS & CONCLUSIONS:The medicine-related amendment of the new "Patent Law" generally reflects an international tendency of restricting pharmaceutical patents so as to maintain public interests,which is in line with the status quo of low technological level of pharmaceutical industry in China.

[Objective] To explore the reasons of catheter-related infections in ICU critical pa-tients and raise up the countermeasures.[Methods] The clinical data of 46 patients with catheter-related infections were surveyed and analyzed from May 2010 to March 2011 in our hospital.[Results] Elderly patients occupied the majority of ICU patients with catheter-related infections.The main infection sites included the respiratory system,urinary system and gastrointestinal system.Establishment of artificial airway was the main cause of infection,indwelling catheter came next.[Conclusions] ICU patients has a high incidence of catheter-related infections.We should handle the ICU management appropriately,strengthen the control and prevention of factors of catheter-related infections,strictly enforce disinfection and segregation regulation,cut off infection way,enhances the resistance of patients,limit antibiotic use and various invasive operation in order to reduce the incidence of catheter-related infections.

Objective To study the nursing methods of gastrointestinal cancer patients undergoing continuous hyperthermic peritoneal peffusion chemotherapy (CHPPC) in the peri-operative period.Methods A total of 126 patients with gastrointestinal cancer underwent continuous hyperthermic peritoneal perfusion chemotherapy,and nursing care was implemented continuously,comprehensively and individually.Results All of the patients underwent continuous hyperthermic peritoneal perfusion chemotherapy successfully,without any complication occurred.Conclusions Intensive and effective nursing care is helpful to carry out continuous hyperthermic peritoneal perfusion chemotherapy safely,and avoid the complications.

Objective:To summarize the nursing experience of a patient with hyperthyroidism-induced liver injury combined with type 1 diabetes complicated by Wernicke encephalopathy.Methods:Patients with hyperthyroidism-induced liver injury, type 1 diabetes and Wernicke encephalopathy co-existing consciousness disorders, contradiction between medication and dietary care, separation anxiety, and specificity of drugs shared by the above three diseases were given appropriate care and discharged health education and continued care.Results:After active treatment and nursing, the patient′s consciousness became clear, nausea and vomiting improved, and liver function and thyroid function indexes gradually returned to normal. The patient was treated with oral 131I and discharged without any adverse effects. Conclusions:The patient′s condition is complex and the clinical care is different from that of ordinary patient. The nurses detect the change of the condition early, adopt a personalized nursing plan for the contradiction between medication and diet of the patient, pay attention to psychological care, reduce the occurrence of complications, and ensure the smooth discharge of the patient.

Objective　To investigate the expression of endometrial leukaemia inhibitory factor（LIF） gene in patients with unexplained infertility. Methods　 By a quantitative reverse transcription-polymerase chain reaction (RT-PCR)，the expression of LIF gene on endometrium during mid-luteal phase was detected in 35 unexplained infertility (infertility group) cases and 20 infertile cases due to tubal obstruction or male factor (control group). Results　The level of LIF mRNA expression on endometrium during mid-luteal phase in infertility group was 0.448±0.239，significantly lower than those in the control group （1.093±0.761，P<0.01). Conclusions　Our findings suggested LIF might play an important role in the process of implantation. The decreased expression of LIF gene might be one of the major causes of unexplained infertility.

Objective To understand Present condition of separation of Pseudomonas aeruginosa and its drug-resistant tendercy in patients with lower respiratory trace infection in our hospital.Methods To make a statistical analysis of durg sensitive tests of 182 strains of Pseudomonas aeruginosa form thos samples of patients with lower respiratory trace infection detected from October 2005 to August 2007.Results 1031 strains of pathogenic bacteria were separated from 1409 sample of lower respiratory trace tract,and pseudomonas aeruginosa 182 strains and 17.65% of them are PA,results of drug sensitive test indicate that Paare resistant to gentamycin CN SMZ CTX GAT etc.Conclusion The infection and drug resistance of pare wery severe now,it isbeneficial to rational use of drugs to keep abreast of current drug-resistant condition.

Objective To investigate the difference between two substrains,5-8F and 6-10B,of nasopharyngeal carcinoma cell line SUNE1on expressing metastasis-associated gene 1 (MTA1),and evaluate the silencing effect of lentivirus-mediated RNA interference (RNAi) on MTA1 in human nasopharyngeal carcinoma cell line.Methods The differential expression of MTA1 mRNA and MTA1 protein in 5-8F and 6-10B cell lines were detected by real time PCR and Western blotting respectively.Short interference RNA (siRNA) fragment targeting MTA1 gene was designed using online databases and software.A specific RNAi lentiviral vector targeting human MTA1 gene was constructed and transfected into nasopharyngeal carcinoma cell line 5-8F.Real time PCR and Western blotting were performed to detect the expressive changes in MTA1 mRNA and MTA1 protein in the transfected 5-8F cells respectively.Results Compared with cell line 6-10B,the expressions of MTA1 mRNA and MTA1 protein were higher in 5-8F cell line.Sequence analysis validated the correct insert of siRNA targeting MTA1 gene into the lentiviral vector.Real time PCR and Western blotting results showed that the expressions of MTA1 mRNA and MTA1 protein in 5-8F cell lines were down-regulated significantly after siRNA transfection.Conclusions MTA1 may promote the malignant transformation and enhance the metastatic potential of nasopharyngeal carcinoma cell lines.The expression of MTA1 in 5-8F cells can be inhibited effectively by MTA1-specific siRNA expression lentiviral vector,which provides a valuable tool for investigating the role of MTA1 gene in the carcinogenesis and progression of nasopharyngeal carcinoma.

Objective To study the route of intrauterine infection of chlamydia trachomatis (CT) Methods Seven hundred and seventy two cervical samples from in women and 105 matched maternal labom neonatal samples composed of cervical samples,cord blood, amniotic fluid, conjunctival and nasopharyngeal samples of neonate were detected by PCR SSCP and DNA sequencing technique Results CT were detected in 87 of 772 (11 3%) cervical samples In the 81 matched maternal infant samples from pregnant women with cervical CT positive, CT were not detected in all of the cord blood samples In the 30 CT positive neonatal samples, 26 were from cases of vaginal delivery and 4 from cases of caesarean section Statistical analysis showed a significant difference between the groups of caesarean section and the vaginal delivery ( P