Objective To establish a HPLC method for determining the content of Paeonol in Paeonia lactiflora Pall.. Methods Ultrasound and reflux methods were used to extract Paeonol in Paeonia lactiflora Pall., and then the content of Paeonol was determined by HPLC. It was performed on Agilent column ZORBAX SB-C18 (Stable Bond Analytical 4.6 mm?250 mm, 5 ?m), mobile phase was methanol-water (45∶55), flow rate was 1 mL/min, column temperature was 25 ℃, and detection wavelength was 274 nm. Results The regression equation was Y=1.01?105X-9.84?102, r=0.999 9 (n=5). The standard curve was linear within the range of 0.104～0.52 ?g for Paeonol. Conclusion The method is simple and accurate with good reproducibility. It is suitable for determining the content of Paeonol in Paeonia lactiflora Pall. and control the quality control of Paeonia lactiflora Pall. in process.

Objective: To study the relationship between mental health and achievement motivation of teachers.Methods: 322 high school teachers completed SCL-90 and Achievement Motivation Scale.Results: The percent of high-score teachers with at least one SCL-90 subscale scored more than 3 is more than 5%.There is obvious correlation between the score of SCL-90 and the AMS's.Conclusion: The high level of achievement-seeking or the high level of failure-avoiding is harmful to the mental health of teachers.

Objective To explore the methods of identifying factitious/fraudulent disorders (M?n-chausen syndrome), enhancing the understanding of their predisposing factors, clinical features, and underlying psychopathology. Method After a final diagnosis of factitious fever was made, data of 4 patients presenting with a puzzling fever of unknown origin were analyzed and compared with those of other cases reported in the English literature. Result It was confirmed that the elevated temperature recorded in the clinical charts of the 4 patients was faked by manipulating of the thermometer. Conclusion These 4 cases show the existence of factitious/fraudulent disorders in China, possibly related to an abnormal psychology, but the tricks employed by the patients are relatively simple compared with those used abroad.

Objective To research the early effect and side effect on the radiotherapy of nasopharyngeal carcinoma patient using IMRT compared with conventional radiotherapy. Methods Seventy eight nasopharyngeal carcinoma patients were divided into IMRT group (38 patients) and conventional radiotherapy group (40 patients). After CT-sim, the radiation oncologist and physician delineated the outline of GTV, CTV, parotid gland, spinal cord and brain stem. The receiving volume of parotid, spinal cord and PTV was respectively calculated by using inversing plan. Face-neck, supracalvicular field, amte-aural field and postaural field were adopted to receive electrons during conventional radiotherapy. Seven to nine conformal fields including 80-100 sub-fields were adopted during IMRT. The reaction of skin, parotid (xerostomia) and oral mucosa were recorded during radiotherapy. Results The nasopharyngeal and para-nasopharyngeal lesions regressed completely after radiotherapy. The volume received by the parotid, spinal cord and PTV was 30%?4.8, 56%?6.7, 95%?8.6, 92%?8.1 respectively in IMRT group, and 98%?9.4, 56%?10.7, 100%?9.7, 99%?9.5 respectively in conventional radiotherapy group. The received volume of parotid in IMRT group was significantly lower than in conventional radiotherapy group. The reaction of skin was grade I in IMRT group and grade II, III in conventional radiotherapy group. The reaction of parotid (xerostomia) was grade I and II in IMRT group and grade II and III in conventional radiotherapy group. The reaction of oral mucosa was grade I to II in IMRT group and grade II and III in conventional radiotherapy group. The reactions of skin and parotid (xerostomia) were lighter in IMRT group compared with those in conventional radiotherapy group. The time of radiotherapy was 45?4.4 days in IMRT group and 51?5.3 days in conventional radiotherapy group respectively. Conclusion The radiotherapy using IMRT regime can decrease the reaction of parotid (xerostomia) and skin to complete the radiotherapy course of nasopharyngeal carcinoma patients. Moreover, it can increase the life quality in nasopharyngeal carcinoma patients after radiotherapy and also shorten the time of radiotherapy.

OBJECTIVE:To compare the efficacy and safety of desloratadine citrate disodium and acrivastine decremental thera-py in the treatment of chronic urticaria. METHODS:132 patients with chronic urticaria were randomly divided into group A(62 cases)and group B(70 cases). Group A was orally given 8.8 mg Desloratadine citrate disodium tablet in 12 2 weeks,once a day;8.8 mg in 3-4 weeks,once every 2 days;8.8 mg in 5-6 weeks,once every 3 days;8.8 mg in 7-8 weeks,once every 4 days;and 8.8 mg in 9-10 weeks,once every 5 days. Group B was orally given 8 mg Acrivastine capsule in 12 2 weeks,3 times a day;8 mg in 3-4 weeks,twice a day;8 mg in 5-6 weeks,once a day;8 mg in 7-8 weeks,once every 2 days;and 8 mg in 9-10 weeks, once every 3 days. The treatment course for both groups was 10 weeks. Clinical efficacy,and plasma histamine levels and total scores of signs and symptoms before and after treatment in 2 groups were observed,and recurrence and incidence of adverse reac-tions after 4 weeks of stopping drugs in 2 groups were followed-up. RESULTS:There were no significant difference in the total ef-fective rate and incidence of adverse reactions between 2 groups(P>0.05). After treatment,the total scores of signs and symptoms in 2 groups were significantly lower than before,the difference was statistically significant(P<0.01),and there was no significant difference between 2 groups(P>0.05). The recurrence rate in group A was significantly lower than group B,the difference was sta-tistically significant(P<0.05). CONCLUSIONS:Both efficacy and safety of desloratadine citrate disodium and acrivastine decre-mental therapy in the treatment of chronic urticaria are good,however,desloratadine citrate disodium is better than acrivastine in re-ducing recurrence rate.

Objective To decrease radiation induced toxicities especially mucostis in patients with locally advanced nasopharyngeal carcinoma( NPC ) who underwent concurrent radiochemotherapy, the maximum tolerated dose and dose limited toxicities of capecitabine combination with cisplatin were observed. Methods From Aug 2006 to Oct 2007, 24 patients with intensity modulated radiotherapy(IMRT) and concurrent chemotherapy with capecitabine and cisplatin for nasopharyngeal carcinoma(stages Ⅲ-Ⅳ) were enrolled in this study. There were four dose-level groups of Capecitabine[625-1250 mg/(m2 ·d) , d1-14]and fixed cisplatin dose[20 mg/(m ·d) ,d1-5) ]MRI and CT scan were used for evaluation of tumor shrinkage. Treatment related toxicities were evaluated according to the common toxicity criteria( NCI-CTC Version 3.0). Results The acute side-effects include Grade 3 or Grade 4 mucosal toxicity(lasting for at least 5 d) and Grade 3 or Grade 4 non-mucosal toxicity were evaluated. Group 625 mg/m2 and Group 825 mg/m2 had none, Group 1000 mg/m2 had 6 patients and Group 1250 mg/m2 had 3 patients for mucosal toxicity, which were the main dose-limited toxicity and relevant to the dose of capecitabine apparently( P < 0. 05 ). There was also a trend of increase by the dose level of capecitabine for other toxicities. The median follow-up time for all patients was 28. 5 months. The locoregional recurrence occurred in 2 patients and distant metastasis in 2 patients. Two-year overall survival rate and locoregional control rate were 100% and 91.7%, respectively.Complete response and partialresponse were found on MRI or CT scan in patients of 29. 2% at the end of treatment and 83. 3% after three months, respectively. Conclusions The combination regimen of capecitabine and cisplatin is safe and effective according to the preliminary result. Toxicities related to radiochemotherapy for NPC were significantly associated with the dose level of chemotherapy.

BACKGROUND: Neurotrophin-3 (NT3) and neurotrophin-4 (NT4) play an important role in the growth, survival and functions of neurons in vivo or in vitro. However, it has not been reported whether they can protect and repair neurons of spinal cord after crushed spinal cord injury (cSCI).OBJECTIVE: To explore the expression of NT3 and NT4 in ventral and dorsal horns of spinal cord at various time points after cSCI and analyze the pattern of expression changes.DESIGN: Randomized controlled study and single analysis of variance.SETTING: Continuing Education College of Beijing Union University;Department of Anatomy, Kunming Medical College.MATERIALS: The experiment was conducted in the Institute of Neuroscience of Kunming Medical College from January to March 2003.Totally 24 adult SD rats were divided randomly into 4 groups: control group, 24-hour cSCI group, 7-day cSCI group, and 21-day cSCI group with 6 rats in each group.METHODS: After anesthesia, rats in each cSCI group were dissected at vertebra T11 and crushed at spinal cord T13. After 24 hours, 7 days and 21days, the rats were killed respectively by cutting their heads off, and spinal cord L1-L3 was taken out to make frozen transactional slices of 20 μm.Rats in control group received no treatment and were killed at the same time point as those in 24-hour cSCI group, and the process of slice making was the same as mentioned above. The distribution of NT3 and NT4 positive cells in the spinal cord ventral and dorsal horns of adult rats was observed, and NT3 and NT4 positive nucleus in the ventral and dorsal horns of the same areas were counted.neurons in the rats' spinal cord ventral and dorsal horns.products were mainly stained in nucleus while NT4 was stained in horns of spinal cord in each group: The number of positive neurons in the ventral horn was significantly greater in 7-day and 21-day cSCI groups than in control and 24-hour cSCI groups (10.2±1.1, 11.4±3.2, 6.2±1.8, 7.4±2.4, P＜0.01). The number of positive neurons in the dorsal horn in 21day group was significantly greater than that in control group (86.4±9.8,71.3±8.3, P＜0.01); however, it was remarkably lower in 24-hour and 7day cSCI groups than in control group (48.5± 5.1, 41.5±3.7, 71.3± 8, P cord in each group: The number of positive neurons in the ventral horn was significantly higher in 24-hour, 7-day and 21-day cSCI groups than in control group (9.4±2.8, 10.8±2.7, 15.1±4.0, P＜0.05); and it assumed an increasing tendency as time went by (P＜0.05). By contrast, the number of positive neurons in the dorsal horn in 7-day and 21-day groups was significantly higher than in control and 24-hour groups (28.1±3.1,35.1±4.4, 23.3±2.3, 24.1±1.8, P＜0.01), and it would increase with time following cSCI (P＜0.01).CONCLUSION: The number of NT3 and NT4 positive neurons increases in spinal cord ventral and dorsal horns after cSCI, suggesting that endogenous NT3 and NT4 have effect at different time on sensory and motor neurons in the repair of spinal cord injury.

BACKGROUND: Multiple sclerosis(MS) is a chronic autoimmune disease induced by the interaction between genetic and environmental factors. Its pathogen and the mechanism of the relapse and remission m the course of the disease are still unknown. Most of the MS research centers are looking for the pathogenic polypeptide epitope in proteolipid protein(PLP), myelin sheath basic protein (MBP) and oligodendrocyte glycoprotein (MOG) OBJECTIVE: To compare the proliferation of T cell lines(TCL) in MS induced by myelin sheath and delipidated myelin sheath towards 11 components of myelin sheath to mainly search the possible pathogenic polypeptide epitope in PLP, and investigate the possible effects of abnormal dcgrease in myelin sheath.DESIGN: A case-controlled trial.SETTING: Department of neurology in a hospital of a university.PARTICIPANTS: Mononuclear cells(MNC) of 16 MS cases(clinical relapsing-remitting type, patients did not receive any immunosuppresant for at least 3 months when their peripheral blood samples were taken) and 12 HLA-DR15 healthy volunteers were furnished by Dr. Trotter JL of MS Research Center of Washington University from the cell database.INTERVENTIONS: MS-TCL and normal TCL were induced twice by stimulation with myelin sheath and delipidated myelin sheath in vitro by cell culture in vitro. TCL proliferation was tested by 11 antigens including PLP,MBP, M87-106, P30-49, P40-60, P89-106, P95-117, P117-137,P139-151, P178-191, and P185-206.MAIN OUTCOME MEASURES: Difference of scintillation counting in every minute of every well, and the stimulative index of each well were calculated, and the mean wells with positive proliferation of TCL towards each antigen were confirmed as well.RESULTS: The general specific proliferation towards myelin sheath antigens was bigger in MS group than control group 5.49 ±5.31 to 3.10 ± 3. 17, and delipidated myelin sheath-induced TCL was bigger than myelin sheath-induced one 5. 49 ± 5.31 to 3.41 ± 4. 83 . Delipidated myelin sheath significantly changed the immune responses of MS group,especially the changes of responses towards P30-49, P40-60, P89-106,P117-137, P139-161, and P185-206 were significant compared with that the control group only responded to two polypeptides, which indicated that the antigen epitope of MBP, PLP, M87-106, P95-117, P40-60, and P185-206 might have significance in the triggering of MS autoimmune responses.CONCLUSION: TCL induced by MS myelin sheath has different proliferation towards antigen components of myelin sheath from control group. Delipidated myelin sheath significantly increases TCL proliferation in MS group, which suggests that if MS patients developed abnormal degrease in myelin sheath, TCL would produce autoimmune response towards self-myelin sheath, MBP, PLP and its polypeptide segments all can trigger MS or aggravate the state of the illness. Our finding supports the hypothesis of MS autoimmune pathogenic mechanism.

Objective:To analyze the drug use in a patient with Crohn’ s disease and provide relevant pharmaceutical service by clinical pharmacists. Methods:Clinical pharmacists participated in the treatment of the patient. The medication was analyzed according to the check results and diagnosis and treatment process, and relevant pharmaceutical service was provided for the patient and nurses. Results: Through the participation in the patient treatment and health education for the patient and nurses performed by clinical pharmacists, the patient was recovered and discharged. Conclusion: Clinical pharmacist participating in pharmaceutical service can promote rational drug use in clinics.

Aim To investigate the hippocampal neuronal injury induced by ?-amyloid peptide ( A?) in rats and the candidate contribution of JNK signal transduction pathway to A? toxicity. Methods Rats were bilaterally injected with A?1 -40 into hippocampi CA1 area. The pathological changes,survival and apoptosis,and ultrastructure of hippocampal neurons,as well as p-JNK positive cells were observed by HE staining,Nissl staining,TUNEL staining,immunohistochemistry and transmission electron microscopy,respectively. Results Thepyramidal cells in hippocampus arranged loosely with decreased cell number. The gliacytes significantly proliferated. Significant apoptotic features were observed in CA1 ultrastructure. TUNEL-positive cells and p-JNK-positive cells significantly increased ( P