Objective:To find out the impacting factors in uncertainty by analyzing the measurement uncertainty in the determina-tion of irbesartan tablets by HPLC and provide the evidence for the measurement evaluation. Methods:A mathematic model for calcu-lating uncertainty was established, and every component in the uncertainty was assessed. Results:The expanded standard uncertainty for the HPLC determination was 2. 4%. Conclusion:The measurement uncertainty of the experiment is mainly caused by the measur-ing method.

Gastrointestinal motility disorders are commonly seen digestive system diseases in elderly subjects. During the process of normal aging,digestive tract experiences a series of degenerative changes,which is the foundation of many gastrointestinal motility disorders. Further study on gastrointestinal dysmotility and its mechanisms is of great significance for the diagnosis and treatment of related diseases in aging population. In this article,the advances in studies on effects of aging on motility of digestive tract( including oropharynx,esophagus,stomach,small bowel,colon and anorectum)and its mechanism researches were reviewed.

Objective:This article provided one splicing technology of medical microscopic image which bases on the comer.Methods:Firstly,use the Harris and MIC algorithms to detect comer.Secondly,use the correlation analysis to obtain the matching points.Lastly,do the splicing and fusion according to the matching points.Results:Achieve the images enhancement and stitching.Conclusions:The experiment proved that the technology can obtained the ideal splicing effect when splicing to the medical microscopic image.

Objective To investigate the expression ofpodocalyxin(PCX) in patients with diabetic nephropathy (DN) and its clinical significance.Methods A total of 68 patients with DN were enrolled as DN group.Another 20 healthy person were selected as control group.The patients with DN were divided into three sub-groups:normal albuminuria group(＜ 30 mg/g,group A),microalbuminuria group(≥30 mg/g but ＜ 300 mg/g,group B) and macroalbuminuria group (≥ 300 mg/g,group C).The level of urinary PCX was measured with enzyme-linked immunosorbent assay.Results The level of urinary PCX,serum creatinine and blood urea nitrogen in DN group was higher than that in control group [(27.44 + 6.87) μ g/L vs.(3.52 +1.13) μ g/L,(154.82 ± 11.54) μ mol/L vs.(82.56 ± 5.71) μ mol/L,(8.79 ± 0.58) mmol/L vs.(4.23 ± 0.34)mmol/L,P ＜0.01].The level of urinary PCX in group A,B,C was (18.04 ±4.12),(23.58 ±5.43),(32.18 ± 6.05) μ g/L,and there was significant difference among three groups (P ＜ 0.05).The level of serum creatinine and blood urea nitrogen was (138.54 + 8.66),(142.16 ± 11.74),(165.13 ± 12.85) μ mol/L and (8.48 ± 0.43),(8.56 ± 0.61),(10.04 + 1.17) mmol/L in group A,B,C,there was no significant difference between group A and group B(P＞ 0.05),but there was significant difference between group A and group C,or between group B and group C (P ＜ 0.05).Conclusion The high-level expression of urinary PCX is found in DN patients,which maybe play a key role in the early diagnosis and assessment of the progression of DN.

Objective To discuss the effect of night- shift work on marital quality, and analyze the relations between the marital quality and related factors in order to explore the method to tackle the marital crisis of night- shift nurses. Methods 500 nurses were selected in general hospitals of Liaoeheng above county-level, each tested nurse filled out marital quality inventory, general well-being scale,social support scale and Eysenck personality questionnaire(EPQ), and withdrew 416 copies(effective rate 90.83%) of ef-fective questionnaire after filling independently. Results (1)There were significant difference in the var-ious scores of marital quality between the nurses and the norm. (2) The marital quality was correlated with general well-being, social support and psychoticism, neuroticism and lying degree of EPQ.(3) Multivariable linear regression which took marital quality as dependent variable ,the variables which entered the equation were age of marriage, social support, psychoticism,subjective well- being,lying degree and objective support. Conclusions (1) The marital quality of night-shift nurses was low. (2)Marital quality was positively cor-relat-ed with subjective well-being, social support and lying degree, negatively correlated with neuroticism and psychoticism. (3) Marital quality were influenced by time of marriage, social support, psycholepsy, sub-jec-tive well-being, lying degree and objective support, among which social support has a great influence.

Objective To investigate the effect of alternating shift on general well-being of nurses,and analyze its relationships with related factors. Methods Alternating shift nurses from general hospi-tals above courty-level in Liaocheng city who were engaged in clinical work were selected by duster sam-pling.Questionnnires were sent out and later gathered. The investigation tools included general well-being schedule(GWB) ,marital quality inventory, social support inventory, Eysenck personality questionnaire were used in survey. Results (1)There was difference in the score of general well-being between nurses with different ages and professional titles(P<0.01). (2)There was significant difference between GWB and oth-er factors such as marital quality,EPQ (psychoticism, neuroticism and introversion and extroversion), so-cial support(P<0.01). (3)Multivariable linear regression which took general well-being as dependent vari-able, the variables which entered the equation were neuroticism, family relationship,support utilization,psychoticism, objective support, introversion and extroversion, marriage satisfaction,arrangement of econ-omy. Conclusions (1)The older, the higher of professional tltles,the stronger general well-being of nurses. (2)Sabjective well-being and marital quality, social support, lying defense were positively corre-lated; neuroticism, psychoticism were negatively correlated. (3)Neuroticism, family relationship,support and utilization, psychoticism, objective Support, introversion and extroversion, marriage satisfaction,ar-rangement of economy can affect general well-being,among which neuroticism had great influence.

Object To study the antiinflammatory effect of matrine and its active mechanism. Methods The matrine effects on activities of secretory phospholipase A 2 (sPLA 2) in peripheral serum and cytosolic phospholipase A 2 (cPLA 2) in leucocyte were measured with Escherichia coli membrane incorporated by arachidonic acid as the substrate; the Ca 2+ level of leucocyte was determined using Frua2 AM loading method, and the rat plantar swelling test was used to examine the antiinflammatory effect of matrine. Results One hour after ip 30 mg/mL matrine, the inhibitory rate of sPLA 2 activity was (81.9?1.8)% , cPLA 2 (28.4?6.0) %, while Ca 2+ concentration was (157.10?20.56) nmol/L which was 15.3% higher than that of the control. Plantar swelling test showed that matrine had a significant anti inflammatory effect. Conclusion Matrine is a novel PLA 2 inhibitor with anti inflammatory effect.

Objective To examine the heating capability of thermoseeds in isolated hepatic tissues.Methods Isolated hepatic tissues were heated uniformly to 37 ℃,meanwhile the thermoseeds and thermocouples were planted using a template paralleling with the magnetic line.Afterwards,the tissues were treated in an extracorporeal magnetic field of 120-123 gauss at a frequency of 113-116 kHz for 30 minutes.At the meantime,the temperature was recorded by a computer through thermocouples. Results The temperature of the tissues raised markedly with a largest change of 14 ℃.The heating area became hard and showed a gradual change of color(white-yellow-brown).The final temperature was negatively correlated with distance when using same number of the thermoseeds(r=-0.96017 to-0.99767,P=0.0023 to 0.0398),and positively correlated with the number of thermoseeds when measuring at a same point(r=0.93775 to 0.99270,P=0.0007 to 0.0185)except for the center group(r=0.93936,P=0.0606).Conclusions Thermoseeds inductive heating technology can achieve an appropriate temperature in isolated hepatic tissues.In certain extent,the temperature increase the number of thermoseeds and heating distance.

Objective To study changes of telomerase activity in transitional cell carcinoma after intravesical instillation of Serratia Marcescens . Methods TRAP-PCR and TRAP-sliver staining were used to detect telomerase activity in 20 patients with TCC after intravesical instillation of Serratia Marcescens . Results Positive rate of telomerase activity detection in TCC patients after intravesical instillation of Serratia Marcescens was 50%(10/20),significantly lowed than before the intravesical instillation (90%,18/20), P

OBJECTIVE:To evaluate inpatients' use of antibiotics in our hospital.METHODS:The inpatients' use of antibiotics in our hospital during 2004～2006 was analyzed retrospectively in respect of the consumption sum and DDDs etc.RES-ULTS:The proportion of antibiotics in the total western medicines were decreasing year on year in terms of consumption sum.Cephalosporin,quinolones,lincomycins and penicillins had been dominated the first 4 places during the 3 consecutive years.Leading the list in terms of consumption sum and DDDs was Clindamycin injection during the 3 consecutive years.CONCLUSION:The inpatients' use of antibiotics was characterized by high starting point,excessively depending on empirical treatment,which needs to be further monitored to achieve a rational use of antibiotics.