Objective To evaluate the therapeutic efficacy and toxicity of oxaliplatin (OXA)-based systemic chemotherapy in patients with advanced hepatocellular carcinoma (HCC).Methods Thirty patients with advanced HCC admitted to our hospital from June 2009 to June 2013 were randomly divided into CapeOX group (15 patients,treated with OXA and capecitabine or CAP)and FOLFOX group (15 pa-tients,treated with OXA and calcium folinate or CF,followed by fluorouracil or 5 -FU).The therapeutic effects were evaluated after two cycles of treatment using the Response Evaluation Criteria in Solid Tumors (RECIST 1 .1 ).Assessment criteria included overall response rate (OR),time to tumor progression (TTP),and overall survival (OS)analyzed by Kaplan-Meier survival curves,as well as the toxicity profile of the combination chemotherapy.Comparison of OR was made by chi-square test;assessment of tumor response and toxicity profile was performed by Mann-Whitney U test;OS and TTP were analyzed by log -rank test.Results In the CapeOX group,the OR was 13.3%,and the mean OS and TTP were 10.4 months and 5.0 months,respectively.In the FOLFOX group,the OR was 6.7%,and the mean OS and TTP were 9.0 months and 4.7 months,respectively.The differences between the two groups had no statistical significance in all three parameters (P=0.543,0.606,and 0.769,respectively).Compared with the FOLFOX group,the CapeOX group had significant-ly lower toxicity rates in gastrointestinal tract and myelosuppression (P=0.006 and 0.002,respectively).Conclusions The OXA-based systemic chemotherapy shows anti-tumor effects for advanced HCC,and there is no significant difference in efficacy between the two regi-mens.Decisions regarding the choice of specific treatment should be based on the patients'clinical conditions.

[Objective] Domestic literatures on misdiagnosis of osteonecrosis of femoral head(OFH)in the past 15 years are reviewed to distinguish OFH from other 9 similar diseases.The symptoms in early,middle and late stages of OFH are similar to other diseases,which is easy to be misdiagnosed as OFH.Pathological changes in different hip joint diseases have different specific performances.Careful and discriminative diagnosis is important in clinical practice.

Objective The study aims to assess the health education effects of popular scientific brochure with pictures on infant vulvovaginitis. Methods Parents of female child outpatients aged 0～5 years old with vulvovaginitis of our hospital were selected for health education, mainly about how to prevent vulvovaginitis. Popular scientific brochure with pictures was designed for use. Eighty six parents of female child patients aged 0～5 with vulvovaginitis were randomly divided into the control group and the observation group with 43 people in each group. In the control group, health brochures were issued and face-to-face guidance were carried out. In the observation group, popular scientific brochures with pictures were issued and faceto-face guidance were carried out Phone call investigations were done 4-8 weeks after issuing of education materials. Results Awareness rate, vulvovaginitis cure rate of their female child and satisfaction rate to education method of the observation group were significantly higher than the control group. Conclusions Health education guidance in form of popular scientific brochure with pictures can get more satisfactory effects.

Objective To investigate the factors that influence the patients to understand the stroke they suffering from.Methods 61 stroke patients were investigated with a questionnaire designed by ourselves.ResultsThe age,sex and educated level affected the stroke patients' understanding,but the time after onset,the degree of locomotory injured and hemiplegic side did not.ConclusionDistinguishing direction of rehabilitation should be established according to the age,sex and educated level of patients.

The PBL Teaching Method has implemented in the department of gynecology and obstetrics of Fudan University since 2004.This new method cultivates the students'ability to ask and answer the questions to collaborate,to comiderate problems conpletely and the ability to consult information and solve problems quickly.Teachers and department should improve the teaching and manage level.

Expression levels of TGF-β1 mRNA in renal cortex of control group, diabetic group and taurine group measured by real-time quantitative RT-PCR were (7.0±0.8)×10 -3, (64.4±8.0)×10 -3 and (16.7±2.0)×10 -3, respectively. The corresponding results with end-point RT-PCR method were 0.28±0.12,0.58±0.16 and 0.43±0.10, respectively. Compared with end-point RT-PCR method, real-time quantitative with SYBR Green I was easier, faster, sensitive and specific.

Objective To analyze the characteristic of twenty-item premature behavioral neurological assessment,and to assess the risk factors of neonatal behavioral neurological assessment(NBNA) in premature infants. Methods Total two hundred and seventy-four premature infants hospitalized in Guangzhou Children's Hospital were assessed with the NBNA after correcting age 40 weeks,premature infants were divided into two groups:normal group (n =221 ) and abnormal group (n =53) according to NBNA. The birth history, mother pregnancy situation, the disease during neonatal period and results in hospital were reviewed,and the risk factors of NBNA in premature infants were assessed. Results There were significant differences in gestational age, birth weight, head circumference, body height, Apgar score, resuscitation time, hospitalization time and total gastrointestinal nutrition time between the two groups( P ＜ 0. 05 ). The incidences of superfoetation,hyaline membrane disease, hemorrhagic and hypoxic brain injury, severe infection, hyperbilirubinemia, hypoglycemia and chronic lung disease were different between the two groups( P ＜ 0. 05 ). There were no differences in gender, antenatal hormone use, anaemia, hypotension and evoked potential abnormality (P ＞0. 05 ). Multivariate logistic regression analysis showed that the risk factors of NBNA score in premature infants would include low Apgar score, low birth weight, hyaline membrane disease, hemorrhagic and hypoxic brain injury, severe infection, hyperbilirubinemia, hypoglycemia and chronic lung disease. Conclusion Although the premature infants corrected age 40 weeks,but the capability and initiative muscular tension are still lower than term infant. Low Apgar score, low birth weight, hyaline membrane disease, hemorrhagic and hypoxic brain injury ,severe infection ,hyperbilirubinemia,hypoglycemia and chronic lung disease are important risk factors related to premature behavioral neurological assessment.

Objective This study was to explore the inhibitory effect of shRNA-VEGF - C on growth of human colon cancer cell line Lovo in vitro and vivo. Methods Recombinant VEGF-C short hairpin RNA (shRNA) plasmid was constructed and transfected into Lovo cells. The expression of VEGF-C was detected at mRNA and protein levels by real-time reverse transcription-polymerase chain reaction (RT-PCR). In vivo study, xenograft tumors were established by injecting LOVO cells into nude mice, then shR-NA-VEGF-C were injected into the tumors, the tumor volume and weight and the incidences of lymph node metastasis were detected. Immunohistochemical staining was used to detect the lymphatic microvessel density of colon cancer tissues. Results After transfection of shRNA-VEGF-C, the mRNA of VEGF-C in Lovo cells were down-regulated. Four weeks after injection, the tumor volume and tumor weight in VEGF-C-shR-NA group were significantly smaller than that in empty plasmid group and NS group [(324. 9 ± 64. 8 ) mm3 vs. (553.5±90. 1)mm3 and (570. 1±85.4)mm3; (3.01 ±0.55)g vs (4.65 ±0.65)g and (4.75 ±0. 75)g]. The incidences of lymph node metastasis (30. 1% ) were significantly inhibited compared with empty plasmid group (50. 2% ) and normal saline group (53. 1% ). In shRNA-VEGF-C group, and microlymphatic density (15.5 ± 6. 90) was also decreased compared with empty plasmid group (24. 18 ±6. 45 ), and normal saline group (29. 59 ± 8. 21 ) ( all P <0. 01 ). Conclusion shRNA-VEGF-C can inhibit the growth of LOVO cells in vitro and vivo. VEGF-C may inhibit the lymph node metastasis of colon cancer by suppressing lymphangiogenesis.

OBJECTIVE: To evaluate the in vitro MTT assay for determining the drug sensitivity in fresh human tu- mors. METHODS: We tested the samples from 75 patients with gastric cancer, 55 patients with colorectal carcinoma, 55 patients with bladder cancer and 6 patients with carcinoma of tongue. RESULTS: On the average, the MTT sensitive drug in the above - mentioned first three kinds of solid tumors corresponded with the drug used by clinican experience. The response rate in MTT sensitive group was 73. 8% (48/65) while only 34.0% (17/50) in the control group and 0% (0/10) in MTT resistant group. CONCLUSION: MTT assay appears to be useful in exploring the possible anticancer effect of arsenic trioxide on tongue cancer. This test demonstrats that the MTT assay is clinically useful on an individual basis to optimize chemotherapy.

OBJECTIVE:To study the stability of timolol maleate eye drops and predict its storage period at room temperature METHODS:Timolol maleate was determined by UV-spectrophotometry,initial average rate method was used in predicting the expiry term RESULTS:Stability of the 0 5% timolol maleate eye drops was not so good,expiry term predicted at 25℃ was only 70 days,while at 5℃ was as long as 1 2 years CONCLUSIONS:Initial average rate method is rapid,convenient and precise,so it is suitable for stability study of hospital preparations