Plant alkaloids have anti-tumor activities.In recent years,the anti-tumor mechanisms of plant alkaloid are demonstrated to be related to the block of tumor cell cycle,the induction of tumor cell apoptosis,the inhibition of tumor angiogenesis and multi-drug resistance.In addition,most recently,plant alkaloids are found to inhibit telomerase activity and induce autophagy.Therefore,plant alkaloids may be developed into a sort of potential antitumor drugs.

Objective To investigated the absorbed dose response of Al2O3 dosimeter in water phantom irradiated by 60Co γ spectrum source. Methods The EGSnre simulation program code DOSRZnrc was used to calculate the absorbed dose of the Al2O3 dosimeter and that of the equivalent volume of water in the corresponding position, as well as the absorbed dose conversion factor, irradiated by 60Co photon beams in a water phantom. Simulations were done for a eylindrieal geometry dosimeter (diameter 0.4 cm and height 0.1 cm) and the dosimeter was placed at the centre of the water phantom at different depths. Results The average absorbed dose conversion factor is 1.143 ± 0.006 and changes little with the depth of the dosimeter in the water phantom, and the deviation is less than 1.0 %. Conclusion The absorbed dose response of Al2O3 dosimeter irradiated by 60Co γ spectrum source is steady and is independent on the depth of the dosimeter in water phantom in this research.

[Objective]To investigate the effect of rabbit BMSCs with fibrin sealant on accelerating early tendon healing.[Methods]The BMSCs were isolated and amplified.The rabbits were divided into experimental(E) and control(C) groups.The injury model was a sharp complete transection through the midsubstance of the achilles tendon.The experimental group was implanted with composite of BMSCs and fibrin sealant,The control group was operated with only fibrin sealant.Specimens were harvested at 1,3,6,and 12 weeks for analysis,which included evaluation of gross morphology,cell tracing,histological assessment,immunohistochemistry studies,morphometric analysis,and mechanical testing.[Results]The gross morphology of the tendons showed the experimental group had lesser adhesion and better reactiveness than that in the control group.The fibrin had degraded at 3 weeks.Cell tracing showed that the labeled bone marrow derived mesenchymal stem cells remained viable and presented in the intratendinous region for at least 6 weeks,becoming more diffuse at later timepoints.At 3 weeks,collagen fibers appeared more organized and there were better morphometric nuclear parameters in the treatment group.At 6 and 12 weeks,there were no differences between the groups with regard to morphometric nuclear parameters.Biomechanical testing showed improved modulus in the treatment group as compared with the control group at 3 weeks,but not at subsequent timepoint.[Conclusion]Intratendinous cell therapy with bone marrow derived mesenchymal stem cells following primary tendon repair can improve histological and biomechanical parameters in the early stage of tendon-healing.

Objective To provide a mathematical formula and method for estimating the distance of shooting by uniform shotgun. Method 360 distribution graphs of canister were made on experimental target paper by shooting with No. 12 and No. 16 uniform shotgun (made in China), using No. 1 and No.3 pellets at the distance of shooting from 1m to 12m. The data of graph measuring and experimental distance were analyzed by mathematic theory. Results Six different types of mathematic formula were obtained for deduce the distance of shooting. They are: 12-1 type (choke) ss= -0.2 + 0.44d; 12- 1 type (no choke) ss= -0.94 + 0.63d; 12-3 type (choke) ss= -0.59 + 0.52d; 12-3 type (no choke) ss= -0.36 + 0.34d; 16-1 types= -0.37 + 0.30d; 16-3types= -0.16 + 0.23d. General software was made using Visual Basic 5.0 computer program. Conclusion This method can be used to deduce the distance of uniform shotgun shooting with little error.

The influencing factors of ultrasonic extraction(UE) and ultrasonic-soxhlet extraction(USE) were analyzed based on orthogonal experimental design. The optimum analytical conditions of two methods were determined. The three different methods: UE, USE and traditional soxhlet extraction(SE) were compared by simultaneous analysis with three different soil samples: fresh contaminated soil, soil after microbial remediation and soil after phytoremediation. The optimum condition of the UE was extracting 4 times with chloroform, 15 mL extractant every time. The optimum condition of the USE method was ultrasonic for 10 min at 60 W, followed by soxhlet extraction with 125 mL extractant for 6 h. Compared the three different methods, USE method showed the best result with the highest average extraction rate of 102.98%, the UE method with average extraction rate of 99.69%, and the SE method with low average extraction rate of 97.31%. The research indicates that USE method is effective in determining soil total petroleum hydrocarbons(TPH) within shorter time and higher efficiency and can be used as a method for determining TPH content in different soils.

Background and purpose:More than 70 percent of ovarian cancer patients were diagnosed in the advanced stage. Currently the 5-year disease free survival (DFS) of stageⅢC-Ⅳovarian cancer patients wsa about 10 percent after first line chemotherapy. This study aimed to improve the 5-year DFS by three-step chemotherapy according to the mechanisms of ovarian cancer biological characteristics, cytodynamics and pharmacology. Methods:In arm A, the patients received three-step chemotherapy after primary debulking surgery, step one with paclitaxel plus carboplatin (TC regimen), every 3 weeks for 6 to 8 cycles;step two with etoposide plus cyclophosphamide, every 4 weeks for 6 cycles;step three wit carboplatin plus cyclophosphamide every eight weeks for six cycles. In control arm B, we retrospectively analysed 51 cases withⅢC-Ⅳstage ovarian cancer, who had completely response after standard chemotherapy with six to eight cycles of TC after primary surgery during 2007. We compared the 5-year DFS between the two arms. Results:The 5-year DFS of 15 cases in arm A was 80%(12/15), which was signiifcantly higher than that of arm B (5.9%, 3/51, P<0.01). Conclusion: The three-step chemotherapy after optimal debulking surgery in stageⅢC-Ⅳ can improve the 5-year DFS. This regimen is high efficacy, mild side-effect witn low cost, which deserves further exploration.

Objective To investigate the diagnosis and treatment of portal hypertension caused by cavernous transformation of the portal vein (CTPV) in adults Methods A retrospective study was made on clinical data of 31 adult upper GI bleeding patients with CTPV The diagnosis of CTPV in all cases were confirmed by B ultrasonography or ultrasonic Doppler and by percutaneous splenoportography or selective arteriography Splenic artery and coronary vein ligation plus C graft mesocaval shunt was performed in 12 cases Splenorenal graft shunt was performed in 1 In 8 post splenectomy rebleeding, cases 6 underwent C graft mesocaval shunt, one inferior meso caval shunt and one jejunectomy due to ictopic variceal hemorrhage Six cases received splenocaval shunt 2 splenopneumopexy 1 splenorenal shunt 1 portocaval shunt 1 pericardial devascularization ResultsPostoperativelly varices disappeared or ameliorated in all patients There was no rebleeding and hepatoencephalopathy occurred in follow up of 6 months to 4 years Conclusion Ultrasonic Doppler and percutaneous splenoportography are diagnostic for CTPV in adults Portasystemic shunt plus porta azygous devascularization is the choice of treatment

Objective To explore differently expressed proteins in serum samples obtained from Deficit Schizophrenia as well as Non‐Deficit Schizophrenia patients with proteomic techniques .Methods Make the comparative study of serum proteins between DS and NDS patients with two‐dimensional electrophoresis .The screened proteins were identified by mass spectrometer .Results Dur‐ing 18 differently expressed protein points screened in this study ,15 proteins were identified by mass spectrometer .Among of them , 12 proteins are up‐regulated while 3 proteins are down‐regulated in DS patients group .Conclusion It′s the first time to make the comparative study of serum protein profiles between DS and NDS patients by proteomic analysis .We expect that this study will contribute to better understanding of the pathophysiology of DS and explore the potential disease‐associated biomarker ,improve the future study for clinical medication of schizophrenia .

Objective To study the abnormal alteration of regions in bipolar depression patients,so as to investigate its pathophysiologic mecha?nism. Methods Regional homogeneity(ReHo)technology of resting?state functional magnetic resonance imaging were used to compare 24 bipolar depression patients and 28 healthy controls. Results Compared to the healthy controls,the bipolar depression patients exhibited significantly higher ReHo value in the bilateral superior frontal gyrus,the bilateral middle frontal gyrus,the bilateral medial frontal cortex,the bilateral supplementary motor area,the left paracentral lobule and the left precentral gyrus(all P<0.001). Conclusion The abnormal function in the bilateral superior frontal gyrus,the bilateral middle frontal gyrus,the bilateral medial frontal cortex,the bilateral supplementary motor area,the left paracentral lobule and the left precentral gyrus may associate with the mechanism of pathophysiology in bipolar depression.

Objective:To provide a normal reference range for anogenital distance (AGD) in full-term neonates and to investigate factors possibly affecting neonatal AGD.Methods:Neonates with gestational age ≥37 weeks who were delivered in the Ruijin Hospital Affiliated to Shanghai Jiaotong University School of Medicine from November 2017 to March 2019 were enrolled.General information on mothers and newborns were collected and neonatal AGD were measured.The distance from the male anus center to the base of the scrotum was determined to be the male AGD, and the distance from the female anus center to the posterior labia was recorded as the female AGD.The effects of maternal and neonatal factors on neonatal AGD were analyzed.Results:A total of 1 078 newborns were included, including 586 males and 492 females.Male AGD [(22.90±3.80) mm] was significantly greater than female AGD [(11.80±2.10) mm] ( t=22.316, P<0.05). The AGD of singleton neonates was significantly greater than that of neonatal twins [males: (23.01±3.82) mm vs.(21.18±1.88) mm, females: (11.89±2.08) mm vs.(10.98±1.75) mm, t=26.185, 18.326, all P<0.05]. Neonatal gestational age, birth weight, head circumference and length were significantly associated with AGD (all P<0.05). Maternal factors (including age, height, weight, body mass index, gravidity, parity, occupation, etc.) were not significantly associated with neonatal AGD (all P>0.05). The AGD of 10 children with genital malformation was significantly smaller than that of males with normal genital appearance [(22.89±1.99) mm vs.(23.55±3.78) mm]( t=15.362, P=0.006). Conclusions:The reference ranges of AGD in full-term males and females in Shanghai are(22.90±3.80) mm and (11.80±2.10) mm, respectively.The neonatal gestational age, birth weight, head circumference and length may be the intrinsic factors affecting neonatal AGD.