Objective To examine whether hair cell immunophenotypes could be derived from the central nervous system (CNS). Methods We established cell cultures from embryonic day 14.5 fetal mouse brain tissues, and analyzed changes in immunohistochemical features of the CNS cell cultures by induction of differentiation.with FGF-2 and EGF.Results The results of this study showed that neural progenitors obtained from fetal rat CNS generated hair cell immunophenotypes with expression of epitopes of hair cell marker proteins myosin VIIa in vitro.Conclusion These findings indicate that immature neural progenitors possess the potential to differentiate into hair cell phenotypes. Immature neural progenitors may be useful as materials for cell transplantation therapy for replacement of damaged inner ear hair cells. Key words: central nervous system, hair cell, immunohistochemical,immunophenotype, transdifferentiation.

Objective To control the hazardous effects of excessive arsenic in drinking water on human health. Methods The experiment on the removal of excessive arsenic from drinking water by electric absorb technology (EST) was performed. Results The pilot experiment and experiment in situ showed that, after treatment by EST, the contents of arsenic in source water(0.06-0.33 mg/L) decreased to the levels of

Objective To observe the changes in electrophysiology and ultramicrostructureafter noise exposure in cochlea of guinea pigs. Methods Thirty animals were divided into 4 groups:group 1 was normal animals,while group 2,3 and 4 were animals of 0.5 hours,7 days and 3 weeks after exposed to noise of 120 dB SPL for 2 hours, respectively.The ABR and 40 Hz-AERP were measured before and after exposure. The hair cells were observed by scanning electron microscope.Results The amplitudes of ABR and 40 Hz-AERP disappeared right after noise exposure, however,they returned to normal 7 days and 3 weeks later . The scanning electron microscopy showed the stereocilia of the hair cells were disarranged and some disappeared at the second and fourth circle of the cochlea . The changes of the pathology at the fourth circle were more serious than that of the second circle. Conclusion The stereocilia of the hair cells had recovered after noise exposure.

Humans ; Laryngeal Neoplasms ; pathology ; Laryngectomy ; Neoplasm Recurrence, Local ; Postoperative Period ; Soft Tissue Neoplasms ; secondary

Objective To observe the clinical efficacy of reinforcing and reducing by acupuncturing at lower He-sea points along and against channel's direction in treating gastroesophageal reflux disease (GERD).Method Sixty-one GERD patients were randomized into a treatment group of 34 cases and a control group of 27 cases. The treatment group was intervened by reinforcing and reducing by acupuncturing at lower He-sea points along and against channel’s direction, while the control group was treated with oral administration of Omeprazole tablets. The two groups were intervened once a day, 4 weeks in total. The clinical efficacies in the two groups were compared based on the symptoms scores before and after the treatment.Result The total effective rate was 97.1% in the treatment group versus 85.2% in the control group, and the difference was statistically significant (P<0.05). The relapse rate 6 months after the treatment was 16.7% in the treatment group versus 50.0% in the control group, and the difference was statistically significant (P<0.05).Conclusion Reinforcing and reducing by acupuncturing at lower He-sea points along and against channel’s direction is an effective approach in treating GERD.

0.05). Conclusion All Nourishing Decoction can enhance the immunity of the body, inhibit the growth of tumor.

Objective To observe the protective effect of alcholic extractive of Cornu Cervi against the myocardial damage of acute myocardial infarction model rats. Methods The myocardial ischemia rats model was made by ligaturing their coronary attery, and the indexes including electrocardiogram, the area of acute myocardial infarction and ET of acute myocardial infarction model rats were observed after the acute period of myocardial damage. Results The alcoholic extractive of Cornu Cervi could obviously improve the degree of myocardial ischemia, reduce the area of myocardial ischemia, and lessen the level of ET. Conclusion The alcoholic extractive of Cornu Cervi could protect the myocardial against the damage of acute myocardial infarction model rats in some degree. The mechanism may be related to the reduction of value of ET of myocardial damage.

Objective The purpose of our study was to assess the onset effect of bystander CPR assisted by a smart phone APP or by a dispatcher.Methods 74 volunteers,without any experience in basic life support measures,was randomly divided into three groups:no-assisted group (n=13),dispatcher-assisted group (n=34) and smart phone APP-assisted group (n=27).Under the condition of a simulative SCA,a QCPR mannequin was used to record the hands-off time,hand placement,rate,depth and complete chest recoil after each compression of the three different groups to make analysis.The no-assisted group didn't receive any CPR instruction;the dispatcher-assisted group was instructed to do CPR through the voice from the telephone;the smartphone APP-assisted group was instructed by a preset Android smart phone CPR APP which guided the volunteers to do CPR step by step by words,voice and animations.Rank sum test analysis of each group using SPSS19.0 software.Results In aspects of hands-off time (1.0 second vs.1.5 seconds vs.5.0 seconds),mean rate of compression (121.0 per minute vs.127.5 per minute vs.58.0 per minute),percentage of correct compression rate (99.0％ vs.98.0％ vs.0.0％) and mean depth of compression (33.0 cm vs.26.5 cm vs.16.0 cm),smart phone APP-assisted group performed as well as dispatcher-assisted group (P＞0.05) and was superior to no-assisted group (P＜0.05).Conclusion In terms of increasing the quality of CPR,the smart phone APP can play the same role as dispatcher's telephone guidance,so as to enhance the effect of cardiopulmonary resuscitation.

Purpose:To explore the clinical feature,and treatment of primary cervical lymphoma. Methods:Clinical data and follow-up survey data of 26 cases were retrospectively analyzed with SSPS software. Results:Primary cervical lymphoma could occur in any age group of women. The misdiagnosis rate was as high as 57.69% (15/26 case).Three of 26 patients died,the five years survival rate was 88.46%.Conclusions:Primary cervical lymphoma is rare,which was very often misdiagnosised. The treatment consisted of an combination of chemotherapy,operation and radiotherapy,which could improve curative results and reduce relapse.

Objective To study the pregnant outcome of those women who were over 41 gesta tional weeks. Methods A retrospective study, including 2903 normal nulliparous women who had delivered between July 1,2002, and December 31,2004, was conducted. Totally, 461 cases of prolonged pregnancies were included. The rates of cesarean delivery, fetal distress, and neonatal asphyxia were compared between those over 41 weeks and the others. Results The rates of cesarean delivery (48. 81%) and fetal distress (43. 38%) in prolonged pregnant group were significantly increased than those of the others women(P