OBJECTIVE:To establish the quality criteria of Guben yangxue oral liquid. METHODS:TLC was employed to identify prepared Ligustrum lucidum,Cornus officinalis,Radix Astragali,Radix Rehmanniae Praeparata,and Angelica sinensis,and the content of polysaccharide was determined by ultraviolet spectrophotometry. RESULTS:The TLC spots were clear,well-separated and specific yet without interference from negative control. The linear range of polysaccharide was 0.020 6～0.072 1 mg?mL-1(r=0.999 2) with an average recovery of 98.64%(RSD=0.56%,n=9). CONCLUSION:The established quality criteria is suitable for the quality control of Guben yangxue oral liquid.

Objective To detect the relationship between tumor markers (TM) and prognosis of NSCLC.Methods The level of TM of 178 NSCLC cases,which all accord with the standard of advanced NSCLC come from Guang'an Men Hospital of China Academy of TCM from 2006 October to 2009 March.Was divided into 5 groups:group 1 (0～3 months)、group 2 (4～6 months)、group 3 (7～9 months)、group 4 ( 10～ 12 months)、group 5 ( ＞ 12 months) according to time to progression (TTP).Studied the correlation between TM,TTP and one year survival retrospectively.Results The serum level of Ca125 in TTP 1～5 group was:(227.9± 73.3)U/ml,(83.4± 127.6)U/ml,(74.8 ± 174.5)U/ml,(53.15 ± 26.3)U/ml,(24.69± 8.78)U/ml respectively which indicated that the higher the serum level of Ca125 was,the more quickly the progress of disease would be(P=0.02); The serum level of Cyfra211 [(9.8±9.4)ng/ml,(10± 6.9) ng/ml,(41.55±9.3) ng/ml,(11.98 ± 9.9) ng/ml,(4.1 ± 0.5) ng/ml) ],CEA [ (88.4± 227) ng/ml,(75.8 ± 272) ng/ml,(57.4 ±153)ng/ml,(35.4±52.5)ng/ml,(17.1± 15.4)ng/ml] and Ca153 [(42.6±52.3)ng/ml,(47.6±147.0) ng/ml,(57.4± 153)ng/ml,(19.4± 12.7)ng/ml,(33.7± 14.6)ng/ml] was associated with prognosis,the higher level they were the lower possibility of one-year survival would be (P=0.041 ).Conclusion Ca 125、Cyfra211、CEA and Ca153 had certain value for prognostic judgment and it was of great significance that measuring rational serum tumor markers on judging prognosis of NSCLC.

OBJECTIVE:To observe the effect of Guben yangxue oral liquid on cyclophosphamide (CTX)-induced suppression of the immune and hematopoietic system in mice. METHODS:Immunosuppressive model mice were induced by intraperitoneal injection of CTX 50 mg?kg-1. Different dose groups were Guben yangxue oral liquid at the same time with Zhenqi fuzheng capsules as control. Several index such as peripheral blood cells, the weight of immune organ, serum hemolysin were determined. RESULTS:The levels of white blood cells (WBC,87.3、26.3、7.96 g?kg-1), lymphocytes (L,87.3 g?kg-1), neutrophils (N,87.3、26.3、7.97 g?kg-1), hemoglobin (Hb,87.3、26.3 g?kg-1), red blood cell (RBC,87.3、26.3 g?kg-1), platelets (PLT,87.3、26.3 g?kg-1), sp- leen index(87.3,26.3 g?kg-1), thymus index(87.3 g?kg-1), serum hemolysin (87.3、26.3、7.96 g?kg-1)in CTX-induced immunosuppressive mice were increased after administrated of Guben yangxue oral liquid. CONCLUSION:Guben yangxue oral liquid can enhance the non-specific immunity and humoral immune function of CTX-induced immunosuppressive mice, and it can improve CTX-induced hematopoietic dysfunction significantly.

Objective To evaluate risk factors of in-hospital mortality during extracorporeal membrane oxygenation(ECMO) support for those with refractory cardiogenic shock after aortic surgery.Methods Retrospective analyses were conducted for 35 patients using ECMO after aortic surgery at Beijing Anzhen Hospital,Capital Medical University from September 2009 to March 2016.Demographic characteristics,diagnosis,ECMO related data,including ECMO indication,operation,clinical parameters before and 24 hours during ECMO were collected to set up a database.The patients were divided into survival group and non-survival group according to the prognosis.The risk factors of mortality in hospital after ECMO were analyzed by multivariate logistic regression analysis regression.Results There were 35 adult patients who had received ECMO support with 32 male and 3female.All patients received veno-arterial ECMO (V-A ECMO).ECMO was successfully weaned in 22 patients (62.9％),and 18 patients(51.4％) survived.Multivariate logistic regression analysis revealed that high lactic acid levels at 12 hours after ECMO(OR =1.445;95％ CI:1.026-2.034) and multiple organ dysfunction syndrome(MODS,OR =10.412;95％ CI:2.246-70.798)(P ＜ 0.05) were independent risk factors of in-hospital mortality in adult patients undergoing ECMO after aortic surgery.Conclusion High lactic acid levels at 12 hours after ECMO and MODS were very important factors of patients undergoing ECMO after aortic surgery.The proper identification and management shall improve the prognosis of patients.

Objective To investigate the nutritional management level of Department of Clinical Nutrition in elderly inpatients through analyzing the actual hospital dietary intakes in elderly inpatients in Peking Union Medical College Hospital from 2013-2015.Methods Using continuous sampling, the nutrients contents and the eating rates of hospital meals ordered by the elderly inpatients on the 1st, 15th, 29th of every month from May 2013 to October 2015 were selected.The intake levels of energy, protein, calcium, vitamin A, vitamin B1 , and vitamin C were calculated and compared with the Chinese dietary reference intakes (DRIs).The nutritional in-take differences between diabetes diet and the basic hospital diet were also compared.Results A total of 90 days of hospital dietary data from 8 402 elderly inpatients were included in the study.The average eating rate was (50.1 ±4.2)%, which was lower than the general eating rate of the hospital [ (59.0 ±4.0)%, P<0.001]. Only protein intake from hospital diet reached the requirement target [male (103.1 ±47.3)%, femal (98.3 ± 33.8)%].Individual compliance rates were 63.2%and 59.8%, respectively, while the other kinds of nutrients were lower.The compliance rates of energy [ (73.3 ±26.3)%vs.(62.1 ±38.2)%, P<0.001] and ma-jor nutrient intake [ protein: ( 119.1 ±41.2 )% vs.( 93.3 ±65.1 )%, P <0.001; calcium: ( 55.5 ± 26.7)% vs.(34.3 ±34.2)%, P <0.001; vitamin A: (75.2 ±48.3)% vs.(57.4 ±97.1)%, P<0.001;vitamin B1:(76.4 ±38.2)%vs.(52.1 ±46.6)%, P<0.001;vitamin C:(92.2 ±51.4)%vs. (49.3 ±55.0)%, P<0.001) in diabetes diet group were significantly higher than those in the basic hospi-tal diet group.Conclusions The nutritional intakes from hospital diets may not meet the nutritional require-ments based on DRIs in elderly inpatients.Medical diet designed by physicians and dietitians should be ex-panded to improve the nutritional management level for these patients.

Objective To explore risk factors of influencing operating efficiency of endoscopic submucosal dissection (ESD) for gastric mucosal lesions. Methods The data of 304 cases with gastric mucosal lesion undergoing ESD in Xijing Hospital of Digestive Disease from April 2009 to February 2017 were retrospectively analyzed. The procedure time and complete resection rate ( R0 resection rate ) were regarded as indicators of ESD efficiency. Risk factors influencing procedure time and R0 resection rate were analyzed using Chi-square test and logistic regression. Results Using median procedure time of 45 min as the cutoff value, Chi-square test showed that specimen size ( P=0. 000) , lesion location ( P=0. 001) , and pathological type ( P=0. 003) affected the operation time. Further logistic regression analysis indicated that specimen size (≥40 mm/<40 mm, P<0. 001, OR=3. 748, 95％CI: 2. 247-6. 254) and lesion location (upper or middle 1/3 of stomach/lower 1/3 of stomach, P=0. 001, OR=2. 180, 95％CI: 1. 318-3. 606) were independent risk factors of procedure time. Using R0 resection as outcome measure, neither single nor multiple parameter analysis was statistically significant. Conclusion Specimen size and lesion location are independent risk factors influencing efficiency of gastric mucosal ESD, and could be possibly used to estimate the procedure time of ESD.

Objective: To investigate the clinicopathological, and molecular characteristics of myoepithelial tumors (MTs) of salivary glands. Methods: A total of 37 MTs cases including 13 malignant epithelial tumors (MMTs) and 24 benign epithelial tumors (BMTs) of salivary glands were identified from the archives of the Department of Pathology, General Hospital of Eastern Theater Command, dating from 2006 to 2016. Clinical features, histological patterns, immunohistochemical characteristics and status of EWSR1 gene rearrangement by fluorescence in situ hybridization (FISH) analysis were reviewed in all cases. Results: Clinically, 37 MTs cases mainly occurred in the parotid glands, when most of the patients presented with painless masses. Of the 13 MMTs cases, male to female ratio was 7∶6, and the median age was 62 years old. Of the 24 BMTs cases, male to female ratio was 5∶7, and the median age was 54 years old. Immunohistochemically, 37 MTs cases were positive for CKpan, and at least one myoepithelial marker. Twenty six of 37 MTs cases were analyzable for the EWSR1 gene break by FISH. Based on the previous evaluation criterion, the EWSR1 translocation was detected in 4 cases of 11 MMTs, and 4 cases of 15 BMTs. According to the main histological composition of tumor cells, 4 EWSR1-positive MMTs covered 2 clear-cell cases and 2 epithelioid-cell cases, when 4 EWSR1-positive BMTs covered 2 clear-cell cases, 1 plasmacytoid-cell case, and 1 spindle-cell case. Conclusions Males and females are affected equally. MTs express immunoreactivity for CKpan, and at least one myoepithelial marker. The EWSR1 rearrangement is present in a subset of MTs, with variable morphological characteristics, and has no statistical significance on clinical behavior.

To analyze how the handover were effected by the conditions of manned spaceflight medical support mission through the practice of medical equipment and drugs in Shenzhou-12 and Shenzhou-13 manned spaceflight medical rescue support missions, this article discussed the preparation, organization and implementation in the handover of medical equipment and drugs in the changing of medical rescue teams, summarized the notices in the work of handover, and provided experience for the smooth handover of different manned spaceflight medical rescue teams in the future.

Background Ultraviolet B (UVB) is one of the main causes of cataract formation,and its mechanism is associated with the apoptosis of lens epithelial cells (LECs).MiroRNA-133b (miR-133b) can regulate oxidative stress-induced LECs apoptosis.However,whether miR-133b is associated with UVB-induced cataract is not elucidated.Objective This study was to observe the inhibitory effects of miR-133b on UVB-induced cataract and its regulating mechanism.Methods Twenty 8-week-old C57BL/6 mice were randomized into cataract model group and normal control group.The mouse eyes in the cataract model group exposed to 302 nm UVB for 5 minutes once per day for consecutive 1 week,with the irradiation intensity of 300 W/cm2,and the mice in the normal control group did not receive any intervention.Five mice in each group were sacrificed and 10 eyeball sections were prepared.Human LECs (SRA01/04) were exposed to UVB for 25 minutes and served as UVB-induced group,the cells in the normal control group did not receive any intervention.The UVB-induced cells were inoculated to 96-well plate and divided into 4 groups,and 50 nmol/L miR-133b mimic,miR-133b mimic control agent,miR-133b inhibitor and miR-133b inhibitor control agent were transfected into the cells with lipofectamine2000.The expression of miR-133b mRNA and a target gene BCL2L2,which was identified by online miRNA database (www.mirab.org) in the cells were detected by real-time quantitative PCR to evaluate the transfected efficacy,and the apoptosis of the cells in mouse lens tissue and different transfected groups were assayed by TUNEL.The use and care of the mice followed ARVO Statement.Results The arrangement of LECs was regular and no apoptotic cell was seen in the normal control group,and the apoptotic cells showed the red fluorescence in the cataract model group.The apoptotic rate of human LECs was (43.90±9.30) ％ in the UVB-induced group,and that in the normal control group was (1.08±0.49)％,showing a significant difference between the two groups (t =-7.963,P =0.015).The relative expression levels of miR-133b mRNA in the model mouse lens and UVB-induced human LECs were evidently lower and relative expression levels of BCL2L2 mRNA were higher than those in normal mice and normal LECs (miR-133b mRNA:t =-2.958,P =0.042;t =-6.195,P =0.003;BCL2L2 mRNA:t =3.761,P =0.020;t =12.437,P =0.000).The relative expression level of miR-133b mRNA was significantly increased and the relative expression level of BCL2L2 mRNA was reduced in the miR-133b mimic group in comparation with the miR-133b mimic control group (t=10.883,-5 927.617;both at P＜ 0.01);compared with the miR-133b inhibitor control group,the relative expression level of miR-133b mRNA was significantly decreased and that of BCL2L2 mRNA was evidently increased in the miR-133b inhibitor group (t =-1 606.622,17.556;both at P ＜ 0.01).The apoptotic rate of human LECs was (43.62 ± 9.19) ％ and (17.55 ± 4.24) ％ in the miR-133b mimic control group and miR-133b mimic group,with a significant difference between them (t =-4.462,P =0.011),and the apoptotic rate in the miR-133b inhibitor group was (78.23 ± 12.42) ％,which was significantly higher than (48.01 ±9.68) ％ in the miR-133b inhibitor control group (t =3.324,P =0.029).Conclusions miR-133b can prevent UVB-induced cataract probably by negatively targeting the BCL2L2 expression to regulate the apoptosis of LECs.

Objective:To study the effects of trioxygen pretreatment on cerebral ischemia/reperfusion (I/R) injury in rats.Methods:A total of 24 clean grade male Sprague-Dawley (SD) rats were randomly divided into Sham group, brain I/R group (I/R group) and Ozone pretreatment group (Ozone group), with 8 rats in each group. The animals were routinely fed, and the operation was performed 5 days after the intervention of Ozone group by intraperitoneal injection of trioxygen water (concentration 80 mg/L, 0.01 mL/g), and the Sham group and I/R group were injected with equal volume normal saline. The Sham group only separated the arteries without ligation, and the I/R group and Ozone group established the rat cerebral I/R model. Neurological deficit score (NDS) was performed 2 hours after ischemia and modified neurological deficit score (mNSS) was performed 24 hours after reperfusion. Brain tissue was collected after anesthesia. Cerebral infarction was observed by 2, 3, 5-triphenyltetrazolium chloride (TTC) staining and the percentage of cerebral infarction volume was calculated. Protein expression of metabolic glutamate receptor 5 (mGluR5) and ionic glutamate α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor (AMPAR) subunit GluA2 in cerebral ischemic penumbra was determined by Western blotting.Results:Compared with the Sham group, NDS score, mNSS score and percentage of cerebral infarction volume in I/R group were increased [NDS score: 2.63±0.52 vs. 0, mNSS score: 9.63±1.19 vs. 1.13±0.64, cerebral infarction volume: (41.25±2.93)% vs. 0%, all P < 0.05], and expressions of mGluR5 and GluA2 in penumbra area of cerebral ischemia were decreased [mGluR5 protein (mGluR5/β-actin): 0.44±0.14 vs. 1.00±0.10, GluA2 protein (GluA2/β-actin): 0.23±0.08 vs. 1.00±0.25, both P < 0.05]. Compared with the I/R group, mNSS score and percentage of cerebral infarction volume in the Ozone group were decreased [mNSS score: 7.00±1.20 vs. 9.63±1.19, cerebral infarction volume: (27.23±6.21)% vs. (41.25±2.93)%, both P < 0.05], and mGluR5 and GluA2 expressions in the penumbra of cerebral ischemia were up-regulated [mGluR5 protein (mGluR5/β-actin): 0.81±0.10 vs. 0.44±0.14, GluA2 protein (GluA2/β-actin): 0.76±0.13 vs. 0.23±0.08, both P < 0.05]. Conclusion:Trioxygen preconditioning can alleviate cerebral I/R injury in rats, and its mechanism may be related to the upregulation of GluR5 and GluA2 in the ischemic penumbra.