Objective This study was performed to investigate whether misoprostol (PGE1 analogue) is effective for the restoration of bone loss in female rats with osteoporosis induced by ovarectomy. Methods The model of osteoporosis was established by ovariectomy in rats, and then the rats were given different doses of misoprostol. Bone mineral density(BMD), serum BGP and urine HOP/Cr were measured. Results BMD (0 26?0 03)g/cm 2 and (0 28?0 02)g/cm 2 in the misoprostal treatment groups was significantly higher than that in the control group 〔(0 23?0 02)g/cm 2, P 0 05〕 Compared with the control group 〔(3 02?0 42)?g/L, P 0 05 〕. Conclusions Results of our study demonstrate that the bone loss caused by ovariectomy was restored by misoprostol administration. The effect of misoprostol mainly is due to its promotion of bone formation.

Human CD38, a type II glycoprotein, is widely expressed in kinds of tissues and cells. It has been shown to have a wide range of effects.The multifunctional cyclase function of CD38 effects on the regulation of osteoclasts activation, which maybe important in the therapy of osteoporosis.

At present, there are the pheuomena of asking sor maney fram patients, writing out unnecessary prescription,and getting medicaine without payment among physicians. It is mot accord with the humanitarianism for patients imim China As everyone knows, the medical moraiith of health adimin'strative cadre involve that whether of not the quality of health services correspond to need of patients so, in this paper, the author discusses that how to strengthen the medical morality for health adimin's trative cadres.

[Summary] Lumbar disc herniation is rare in juveniles , which makes it more difficult to diagnose and treat .The prevalence , causes and risk factors , pathological changes , clinical characteristics , main treatment methods , and curative effects of lumbar disc herniation in juveniles were summarized in this review , for benefiting clinical diagnosis and treatment .

Objective To design a new type of posterior lumbar interbody fusion cage made of NiTi shape-memory alloy and to evaluate its histological and biomechanical behavior through animal study. Methods This study contained three steps of experiments which was posterior lumbar interbody fusion using different fusion materials and devices. At the first step, 12 sheep lumbar functional spinal units were randomly divided into 4 groups with 3 specimens in each group. There was one group with autogenous iliac crest bone dowel(IG), one group with interfix lumbar cage(cage), one group with NiTi-TFC(NT) and one group served as control group. Nondestructive biomechanical tests were carried out for each group. At the second step, 15 sheep were randomly divided into three groups with 5 specimens in each group. There was one group with cage and autogenous iliac crest graft, one group with NT and iliac crest and one group served as control group. All the animals were undergone imaging study regularly to evaluate the change of the intervertebral space heights and the process of fusion. At the third step, all animal from the second step were sacrificed 6 months later for histological analysis of the operated segments. All the data were statistically analyzed using SPSS 7.0. Results The strength and axial stiffness of the autogenous iliac crest bone dowel graft group and the control group were significantly different from the NT and cage group(P

[Objective]To evaluate whether transplanted marrow mesenchymal stem cells interfered in TGF-?1 can differentiate to nucleus pulposus cells and increase the amount of proteoglycan and collagenase Ⅱ content in intervertebral discs.[Method]We used an in vivo model to investigate the feasibility of marrow mesenchymal stem cells that cultured in vitro and interfered in TGF-?1 delivery,retention,and survival in the degeneratived disc space.In 2,4,6,8 weeks we used immunohistochemical staining to determine the change of collagenase Ⅱ content;spectrophotometry to determine the change of amount of proteoglycan with Phlorglucinol;the experiment date were analyzed by SPSS 11.5 soft ware.[Result]We found MSCs could maintain viability and proliferate within the rabbit inter vertebral disc.The amount of proteoglycan and collagen Type Ⅱ content of the intervertebral in matrix synthesis in the experiment group was increased in 8 weeks.We found no changes in the modle group.[Conclusion]Our data suggest that transplanted marrow mesenchymal stem cells in vivo can survive and increase proteoglycan and collagen Type Ⅱ amount interfered in TGF-?1 in some periods,which support its potential use as a treatment of intervertebral disc degeneration.

[Objective]To evaluate short term clinic results of prosthetic disc nucleus(PDN)replacement for the treatment of lumbar disc herniation.[Method]Twenty cases of lumbar disc herniation(including one case of recurrent lumbar disc herniation)were treated with PDN from June 2003 to November 2003,including 13 males and 7 females with average age of 40.5 years.All cases were implanted with a single PDN,in which 7 cases with PR725,8 cases with PW725,and 5 cases with PDN-SOLO-7 respectively.[Result]After surgery,one patient accepted the revision operation to remove the PDN because of device migration,the others experienced pain relief.The nineteen cases were followed-up for 23～29 months(mean 26.4 months).Compared with preoperative height of intervertebral disc,it gained increase of 17.2%(P

Objective To investigate clinical features and anterior surgical results of cervical myelopathy in patients more than 70 years of age. Methods Twenty aged patients with cervical myelopathy who underwent anterior decompression with bone graft surgery were reviewed. Neurological function was assessed using a scoring system proposed by the Japanese Orthopaedic Association (JOA score). The clinical results and complications were compared with the control group which included 31 patients with multisegmental cervical myelopathy and less than 69 years old and underwent the same operation. Results The preoperative mean JOA score was 9.3 (ranged from 3 to 14) and the JOA score at latest follow-up averaged 13.4 (ranged from 8 to 17) in the aged patient group. 68 percent patients had achieved an excellent or good result and the recovery rate was 58%. 71 percent patients had achieved an excellent or good result and the recovery rate was 67% in the control group. No statistical difference was found in the excellent and good result rate or the recovery rate of JOA score between the aged group and the control group (?字2=0.04, P=0.85;t=1.12, P=0.138). The incidence of surgical related complications in the aged group was 35% (7 cases), which was considerably higher than that in the control group (3 cases, 10%) but without statistical difference (?字2=3.47, P=0.06). In the preoperative flexion-extension stress lateral radiographs, the incidence of cervical instability was higher in the control group (8 cases, 26%) than that in the aged group(1 case, 5%). Conclusion 1) Multilevel lesion induced by overcompensation for cervical instability are the probable cause of myelopathy in aged patients. 2) Anterior decompression is beneficial to improve the neurological function and life style in the aged patients with cervical myelopathy, but associated with a high incidence of surgical complications.

[Objective]To study the mechanism of posterior longitudinal ligament in cervical spine through measuring the collagen and Proteoglycan and Calcium changes.[Method]Fifteen specimens of cervical longitudinal ligament from cervical spondylotic myelopathy(CSM)and ten control specimens from corpses without cervical spondylosis were obtained.The content of the collagen was measured by Weossner method.Collagen type Ⅰ and Ⅱ were measured by Enzyme-Linked Immunosorbentassay(ELISA)method.Phloroglucinol spectrophotometer to determine the change of amount of Proteoglycan.The Calcium by Methyl-Thymes-Blue(MTB)Colorimetric Method.The specimens were treated hy Hematoxylin and Eosin(HE)stain method and by Masson stain method,the pathological changes of two groups were observed through microscopy.[Result]In CSM,as compared to the control groups,there showed a decrease in the contents of the total Collagen,Collagen type Ⅰ and Proteoglycan,and increase in the content of collagen type Ⅱ.The rate of type Ⅰ/Ⅱ in CSM was lower than that in control groups.An increase in the content of the Calcium.All of which have statistic significance(P

Objective To evaluate the methods and results of revision surgery for posterior lumbar cage interbody fusion (cage-PLIF) with postoperative complications, and to analyse the surgical techniques for prevention of these complications. Methods From October 1996 to December 2002, 21 patients with postoperative complications of cage-PLIF underwent reoperations. There were 11 males and 10 females with an average of 43.4 years. The interval between primary and revision surgery ranged from 6 days to 1.5 years with an average of 0.6 year. 16 patients suffering from lumbar disc herniation were treated with the discecto-my and single uninstrumented cage fusion, 5 patients of lumbar spondylolisthesis were treated with cage-PLIF and pedicle screw instrumentation. The complications included cage displacement backward in 20 patients, forward in 1,and cage subsidence in 9 as well. 15 patients complained of low back pain wors-ening or leg radicular pain, of which 4 had intermittent claudication and 10 had leg numbness or weakness during rehabilitation. Revision surgery included re-implantation of the cage filled with iliac crest bone chips in 11 patients, iliac bone autograft after removal of original cages in 7 and decompression of involved nerve root witbout removal of migrated cage because of technical difficulty. Pedicle screw fixations were used in 12 and the intertransverse fusion both with autograft and allograft was added in 7. Results The mean follow-up was 14.2 months (ranged, 7 to 36 months). The cages presented slight retro-displacement in 4 patients shortly after reoperation, without involvement into spinal canal during the subsequent follow-up. Bony fusion occurred in 13 patients, and the pseudarthrosis in 3 patients without further migration of cages. The clinical symptoms relieved in 5 patients, improved in 9, no any change in 6, and worsened in 1. However, low back pain remained in 8 patients, and dysuria in 1 patiant at the last follow-up. Conclusion The results of revi-sion surgery are not satisfactory according to this study, the surgical treatments should be performed as soon as possible if conservative treatments is ineffective. The correct surgical indication and proper technical are the key of prevention of the postoperative complications.