Objective]To summarize herbal medicine application regulation of Professor Wu Liangcun in gastric cancer treatment. [Methods]Collect 116 gastric cancer patients from Chinese medicine clinic system during January 1,2014 to December 31, 2014 and establish clinical prescription underlying database. Apply descriptive analysis to drug cumulative frequency, drug-induced taste and nature and apply cluster analysis to 42 drugs using more than 10 times. [Results] In the 116 gastric cancer patients,159 kinds of herbs were used ,the cumulative frequency of herbal medicine was 1816.GanCao was the herb with highest usage frequency, and then was FuLing, ShanYao, TaiZishen;According to accumulated frequency, herbs with tonifying function were at the top position(herbs with tonifying qi function were most), and then was the herbs with function of herbs with the function of clearing excessive heat, herbs with function of herbs with the function of relieving dyspepsia, herbs with function of herbs with the function of regulating qi. In terms of herbs' nature and taste, herbs with sweet taste, plain nature were used most. Cluster analysis divided all used herbs into 5 sections, involving prescriptions Sijunzi decoction, Erchen decoction, and couplet medicines DaoYa with GuYa, JiNeijin with MiHoutao, SheShecao with XianHecao and so on. [Conclusions] Professor Wu Liangcun treats gastric cancer with the law of tonifying qi, the main prescription is the same as Sijunzi decoction, emphasizing nourishing Yin and clearing away the heat-evil, protecting gastric, accompanied with promoting digestion and regulating qi.

To explore the role of connexin43 (Cx43) in gap junctional intercellular communication (GJIC) and propagated sensation along meridians, the expression of Cx43 in the rat epithelial cells and fibroblasts was studied both in vitro and in vivo. With the in vitro study, the rat epithelial cells and fibroblasts were cultured together, and the localization of Cx43 was detected by immunohistochemistry and indirect immunofluorescent cytochemistry and under confocal microscopy. And the expression of Cx43 on the surface of the cells was examined by flow cytometry. With the in vivo examination, 20 SD rats were randomized into control group (n = 10) and electrical acupuncture group (EA group, n = 10). EA ( 0.5-1. 5 V, 4-16 Hz , 30 min) was applied to "Zusanli" acupoint for 30 min at rat's hind paw, the localization of Cx43 was immunohistochemically detected. The immunohistochemical staining and indirect immunfluorescent cytochemistry showed that Cx43 was localized on the surface of the cells and in the cytoplasm. The relative expression level of Cx43 on the cellular membrane surfaces of the rat epithelial cells and fibroblasts, as determined by FACS, were 13.91% and 29.53% respectively. Our studied suggested that Cx43 might be involved in GJIC and propagated sensation along meridians.
Cells, Cultured ; Connexin 43/*biosynthesis ; Connexin 43/genetics ; Epithelial Cells/cytology ; Epithelial Cells/*metabolism ; Fibroblasts/cytology ; Fibroblasts/*metabolism ; Flow Cytometry ; Gap Junctions ; Meridians ; Microscopy, Confocal ; Random Allocation ; Rats, Sprague-Dawley

Objective To determine the intestinal absorption characters of zingerone in rats.Methods The concentration of zingerone in the intestine perfusate was determined by HPLC.The absorption kinetics were obtained by the in-situ perfusion method in rats.Results The absorption rate constants(Ka) of zingerone were 0.0137,0.0135 and 0.0139 min-1 at the concentration of 25.0,50.0 and 100.0 ?g?mL-1,respectively;Ka of zingerone in the duodenum,jejunum,ileum and colon were 0.0070,0.0031,0.0032 and 0.0026 min-1,respectively.Conclusion Concentration of zingerone has no significant effect on its absorption kinetics.The best absorption segment of the intestine was duodenum,ileum,jejunum and colon by turns.The intestinal absorption of zingerone has the first-order kinetic characters with passive diffusion mechanism.

Objective To compare the curative effect of the different palm dorsal approach of open reduction and internal fixation for the treatment of distal radius fractures.Methods According to the stochastic indicator method,84 patients with distal radius fractures were divided into control group and observation group,42 cases in each group.The control group was treated with dorsal approach open reduction and internal fixation,while the observation group was treated by volar approach open reduction and internal fixation.The clinical curative effect was assessed,and indicators related to the operation and the fracture healing was recorded in detail.Postoperative complications of patients were analyzed during postoperative follow-up.Results The total effective rate of the observation group was 88.10%(37/42),which was significantly higher than 66.67%(30/42) of the control group(x2=5.509,P=0.019).Compared with the control group,the operation and hospital stay time were significantly shorter,intraoperative and postoperative blood loss of the observation group decreased obviously,and the differences were statistically significant(t=2.092,2.137,2.256,2.199;P=0.040,0.036,0.027,0.031).The Gartland-Weriey score and ulnar inclination of the observation group were (9.18±2.96)points and (10.52±2.64)°,which were significantly lower than those of the control group[(12.56±3.24)points,(16.22±2.87)°],while radial height[(11.61±2.87)mm]was higher than the control group[(10.92±2.65)mm],the differences were statistically significant(t=2.206,2.316,2.185;P=0.030,0.023,0.032).The incidence rate of complications of control group was 26.19%(11/42),which was higher than 9.52%(4/42) of the observation group(x2=3.977,P=0.046).Conclusion The curative effect of volar approach of open reduction and internal fixation in the treatment of distal radius fractures was better compared with dorsal approach.At the same time,it can effectively improve the operation indicators and fracture healing effect,in addition,it can effectively reduce the incidence of postoperative complications.

Objective\ To study the influence of long-term quality of life in MHD patient after their family numbers have received home-nursing education Methods 49 cases of MHD were divided into two groups randomly: the observe groups(n=24),and the control groups(n=25).Both groups were received routing treatment with health education,while the family members of observation groups were also guided with home-nursing education.After one and five years′ treatment,the index of quality of life were compared with SF-36 respectively. Results The quality of life in observation groups was significant higher than that of control group(after one year P

Objective To observe the effects of Danqi Granules combined with Xianling Gubao Capsules on bone metabolism and bone mineral density (BMD) in patients with postmenopausal osteoporotic vertebral compression fractures (OVCF) after surgery. Methods 90 cases of postmenopausal women with OVCF were randomly divided into the control group and the observation group, 45 cases in each group. The two groups were treated by vertebroplasty (PVP), and after the surgery, the control group was treated with Xianling Gubao Capsules while the observation group was additionally given Danqi Granules. The serum markers of bone metabolism [(25 (OH) D, PINP, β-CTX] and BMD of L2-4 and left femoral neck were detected before and after treatment. Results The level of serum 25 (OH) D in the observation group after treatment was significantly higher while the levels of PINP and β-CTX were significantly lower than those in the control group (P<0.05), and the BMD of L2-4 and left femoral neck of the observation group were significantly higher than those of the control group (P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups. Conclusion Danqi Granules combined with Xianling Gubao Capsules can significantly accelerate fracture recovery, and improve bone mineral density in the treatment of postmenopausal women with OVCF.

Objective To analyze the distribution of reproductive tract infection(RTI)pathogens and the drug sensitivity of My-coplasma in infertile women.Methods Experimental examinations of the pathogens related to RTI were performed in 260 cases of infertile women(test group)and compared with 260 cases of pregnant women(control group).Results In test group,the positive rate of RTI pathogens was 61.2%.The top 3 pathogens were Mycoplasma (47.7%),Candida (30.0%)and bacterial vaginosis (BV)pathogens(16.7%).There were significant differences of BV pathogens,Mycoplasma,and pH value between test group and control group(P <0.05).And the differences of other pathogens and the cleanliness were not significant between test group and control group(P >0.05).The drug sensitivity rates of Ureaplasma urealyticum to doxycycline and minocin were above 90%,and which to quinolone was less than 40%.Conclusion BV pathogens and Mycoplasma infection is one of the important factors which could affect women infertility.It is necessary to strengthen the monitoring and rational use of antimicrobial agents.

Objective To observe the clinical effect of the anterolateral thigh flap in repairing defect caused by resection of malignant melanoma in the heel region.Methods 8 patients with heel malignant melanoma were trea-ted by surgery.The malignant melanoma was further diagnosed by histopathological examination.Their heel defects caused by large resection of malignant melanoma were treated by anterolateral thigh flap.Results All skin flaps sur-vived and all the patients could walk normally 1 month after operation.The recovery of the function and sense was well 6 months after operation.Compared with the another well heel,the flaps had normal texture,color and appearance of heel.8 patients had complete follow -up,ranged form 6 months to 3 years.All cases survived with neither local recur-rence nor distant metastasis.Conclusion The anterolateral thigh flap can achieve good clinical effect in treating heel defect caused by the resection of malignant melamoma.And especially apply in large skin and tissue defect of heel area.The flap can weight,wear -resisting and reduce overheating and over cold damage.The range of resection can not be restricted by local skin flap.The clinical effect is satisfied.

Objective To observe therapeutic effects of early scab-cutting combined with axis-like abdo-men skin flap implanting in patients with wrist deep electrical burns.Methods Retrospective study was employed to analyze 70 patients received axis -like abdomen skin flap implanting for wrist deep electrical burns.All the cases were separated with early scab-cutting group(35 cases)and delayed scab-cutting group(35 cases),according to types of skin flap implanting.The therapeutic effect for the two groups were evaluated with rate of infection,duration of healing,living rate of skin flap,grade of skin burning,and rate of amputate.Results In the early scab -cutting group,the rate of infection was 11%,the duration of healing was(24.0 ±6.0)days,the rate of amputate was 3%,the living rate of skin flap was 97.0%,the wound healing of class A was 94.3%.In the delayed scab-cutting group,the data were respectively 31%,(35.0 ±7.4)days,17.0%,31% and 28.6%.Compared with the data of the two groups,the difference had statistical significance(the chi-square value or T value were respectively 4.151,6.831,3. 968,31.895,3.968.All the P values was less than 0.05).Conclusion Early scab-cutting combined with varied sorts of skin flap implanting shown great benefits for treatment of wounded and facilitate rehabilitation in function of wrist,which suggest decrease rate of complications and minimized hospitalized duration.

Objective: To establish a mini invasive way to evaluate endothelium function. Methods: Group 1 consisted of 10 subjects with no risk factors for coronary artery disease, group 2 consisted of 11 subjects with risk factors for coronary artery disease and angiographically normal coronary arteries. Two groups of patients underwent transesophageal echocardiography(TEE) for assessment of LAD diastolic blood flow velocity (V) and time velocity integral (VTI) at rest (B 1), 2 min after immersion of the hand in cold water (CPT), 5 min after rewarming of the extremity (B 2), and 5 min after administered nitroglycerin. The percent change in V and VTI were calculated between B 1 and CTP and B 2 and nitroglycerin. Results: After CPT the percent increase in V and VTI were significant in group 1 than group 2 ( P