1.Expression of G protein inwardly rectifier potassium channels subunit 2 mRNA in hippocampus of temporal epileptic rat
Yu WANG ; Bo XIAO ; Xiaosu YANG
Journal of Clinical Neurology 1988;0(02):-
Objective To explore the expression of G protein inwardly rectifier potassium channels subunit 2(GIRK*!2) in hippocampus of temporal epileptic rat.Methods After temporal lobe epilepsy was induced by kainic acid (KA), we took advantage of in situ hybridization to investigate the altered expression of GIRK*!2 mRNA in rat hippocampus.Results GIRK*!2 mRNA significantly increased in epileptic rats dentate gyrus region compared with normal control( P
3.Cloning and Sequence Analysis of LACK Gene of Leishmania Donovani Isolates from Plain and Desert Foci of China
Ying MA ; Xiaosu HU ; Yajing WANG ; Zilong WANG
Chinese Journal of Parasitology and Parasitic Diseases 1987;0(04):-
Objective To determine the nucleotide sequence of the LACK (Leishmania homologue of receptors for activated protein kinase C) gene of Leishmania donovani isolates from plain foci (L.d SD1) and desert foci (L.d XJ771) of China, and to find out the difference of the sequence of LACK gene with other Leishimania spp. and also the isolate from hill foci of China.. Methods. The LACK genes of L.d SD1 and L.d XJ771 were amplified by RT-PCR and cloned into pUC18 vector respectively, sequenced by the dideoxy chain termination method, then analyzed and compared with that of other isolates.. Results . The LACK genes of the two isolates were successfully cloned. Both of the 2 fragments were 942 bp in length. Comparison of the two nucleotide sequences with that of other Leishmania spp. in GenBank showed that the identities were more than 97%, and the identities of the nucleotide sequences of LACK genes of the three L.d isolates from plain, desert and hill foci of China were more than 95%.. Conclusion . High identities exist among the nucleotide sequences of LACK genes of the three L.d isolates from the three foci of China.
4.Clinical characteristics of patients with reflux esophagitis exhibiting gallbladder heat attacking the stomach or stagnant heat of the liver and stomach syndrome.
Ji SUN ; Shengliang ZHU ; Shuying MA ; Xiaosu WANG ; Jing KONG
Journal of Integrative Medicine 2011;9(7):732-6
To compare the characteristics of the traditional Chinese medicine (TCM) syndromes of gallbladder heat attacking the stomach and stagnant heat of the liver and stomach in patients with reflux esophagitis (RE), in terms of clinical symptoms, combination of gallbladder conditions, esophageal mucosal inflammation, gastric bile reflux under endoscopy and helicobacter pylori (HP) infection.
5.Promoting effect of core competence evaluation on continuing education of training nurses in operation room
Xiaosu HU ; Li GUO ; Qingxin FU ; Jing WANG
Chinese Journal of Practical Nursing 2014;30(20):62-64
Objective To explore the influence of core competence evaluation on continuing education of training nurses in operation room.Methods Questionnaires were administered to 42 training nurses by using the registered nurse core competence scale (CIRN) in operation room.Results The level of core competence of training nurses in operation room was medium (3.25 ± 0.45).Legal and ethical practice dimension of scores was the highest (3.55 ± 0.63).The single average of clinical nursing dimension was the advanced lowest (3.11 ± 0.54).The nurses self identity was the core influencing factors by multiple stepwise regression analysis in regression.Conclusions The core competence of training nurses in operation room is on the middle level,The focus is to improve the ability of clinical nursing in continuing education.To give more attention and support in self identity of the operation room training nurses,and to provide the scientific basis for the training plan and specialist training for them,in order to promote the continuing education projects of training nurses.
6.Evaluation of Preoperative Imaging in Total Mesorectal Excision for Rectal Cancer
Yinghua WANG ; Hui HUANG ; Xiangjiu XIU ; Gang HUANG ; Qianghua MA ; Xiaosu REN ; Jun BIAN ; Yaqun WANG
Journal of Practical Radiology 1996;0(04):-
Objective To study the value of multi-slice spiral CT(MSCT)and high-resolution MR in evaluating the feasibility of total mesorectal excision(TME)for rectal cancer before operation.Methods Sixty-three cases biopsy-proven rectal cancer underwent MSCT and high-resolution MR examination,including CT in 35 and MR in 28.The involvement of mesorectal fascia,lymph nodes extra-fascia and the curve distance between the lesions and the anal margin were observed and compared with that of operation and pathology.Results In evaluating the involvement of mesorectal fascia,two patients were oversteped by CT and one patient by MRI,and there was no significantly statistic difference between CT and MRI.The curve distance between lesions and anal margis could be measured accurately on sagittal section of MR images in all cases.Sixteen patients had been found having metastatic lymph nodes at extra-mesorectal fascia,twelve of them were biopsy-proven.Thirty-seven patients had been found having metastatic lymph nodes at intra-mesorectal fascia,but 4 of them were inflammatory biopsy-proven.Conclusion MSCT and high-resolution MR are of significant values in evaluating the feasibility of TME in the patients with rectal carcinoma.
7.Non-esterified fatty acid level and metabolic characteristics in nondiabetic first-degree relatives of type 2 diabetics
Xiaoli WAN ; Wei REN ; Suhua ZHANG ; Song LIANG ; Lilin GONG ; Xiaosu BAI ; Ruizhi ZHENG ; Maorong WANG
Chinese Journal of Endocrinology and Metabolism 2008;24(2):149-152
Objective To investigate the change of serum non-esterified fatty acid (NEFA) level in nondiabetic first-degree relatives of type 2 diabetics, and to explore the related factors in the change.MethodsSerum lipid profile, plasma glucose and insulin levels were measured in 186 type 2 diabetic patients, 565 nondiabetic first-degree relatives of type 2 diabetics and 149 normal controls. Results (1) The fasting NEFA level in first-degree relatives was significantly lower than that of type 2 diabetic patients [(0.53±0.28 vs 0.63±0.31) mmol/L,P<0.01]and HOMA-IR was significantly higher than that of normal controls (0.98±0.51 vs 0.89±0.47,P<0.01). (2) The fasting NEFA level in the first-degree relatives with higher body mass index (BMI), plasma glucose or area under curve of glucose concentration (AUCglu) was higher than that in those with lower BMI, plasma glucose , blood pressure or AUCglu (all P<0.05). (3) NEFA showed significantly positive correlations with BMI, systolic blood pressure, diastolic blood pressure (DBP), AUCglu in the first-degree relatives by correlative analysis (r=0.12, r=0.148, r=0.21 and r=0.281 respectively, all P<0.05). Stepwise linear regression analysis showed that DBP, AUCglu and age were the independent risk factors of NEFA (all P<0.01). Conclusion Insulin resistance exists in nondiabetic first-degree relatives of type 2 diabetics, which seems to be related to elevated NEFA levels.
8.Altered expression of G protein-coupled inwardly rectifier potassium channels (GIRK) subunit 1 and 2 in hippocampus of chronic temporal epileptic rats induced by kainic acid
Yu WANG ; Bo XIAO ; Xiaoyan LONG ; Xiaosu YANG ; Jinghui LIANG ; Zhiguo WU ; Shuyu LI ; Guoliang LI
Chinese Journal of Pathophysiology 2000;0(10):-
AIM: G protein-coupled inwardly rectifier potassium (GIRK) channel are distributed widely in mammalian brain. In CNS, GIRK 1/2 seems to be the predominant heterotetramers which play a pivot role in the regulation of the excitability of neurons and may contribute to the resting potential by leading to a hyperpolarization of membrane potential and reduction of the action potential frequency. In the context, the Weaver mouse is the first neurological abnormality directly linked to a genetic point mutation in the GIRK2 protein which includes spontaneous seizure. GIRK2 knock out mice showed normal development but more susceptible than normal mice to seizure induced by GABA antagonist. Here, we report that the mRNA and protein expression of GIRK subunit 2 is altered in kainic acid(KA)-induced epileptic rat hippocampus. METHODS: Rats were injected with kainate 14 mg/kg intraperitoneally to establish an acute and chronic temporal lobe epilepsy model. At chronic spontaneous seizure stage, by using of in situ hybridization, immunocytochemistry and Western blotting, the GIRK 1,2 mRNA and protein were analyzed quantitatively in the dentate gyreus, CA1, CA3 regions of hippocampus. RESULTS: GIRK1,2 mRNA and proteins were expressed abundantly in all regions of hippocampus. KA induced seizures and caused a significant increase in GIRK2 mRNA abundance and immunoreacitivity; only GIRK1 mRNA was increased significantly, but no difference was found by Western blotting protocol. CONCLUSION: GIRK1,2 mRNA and protein expression in the hippocampus of epileptic rat brain is up-regulated, which may be an adaptive response to over-excitability of neuron networks and prevent the over-excitability spread in hippocampus (DG-CA3-CA1). [
9.Marburg I polymorphism of Factor Ⅶ-activating protease and cerebral infarction
Qian TAN ; Huarong TANG ; Rongrong LIU ; Guangping WANG ; Xiaosu YANG ; Fangping CHEN
Journal of Central South University(Medical Sciences) 2009;34(12):1171-1175
Objective To determine the relation between Marburg I polymorphism of FactorⅦ-activating protease (FSAP) and cerebral infarction,and to analyze whether it is one of the risk factors of cerebral infarction.Methods Single strand conformation polymorphism-polymerase chain reaction (SSCP-PCR) was applied for the polymorphism analysis of FSAP in 159 patients with cerebral infarction and 179 non-cerebral infarction subjects.Results The phenotypes of FSAP in both the patients and the control subjects were wild type GG;no mutant of Marburg I was found. But a new gene mutation was tested, which had not been reported, requiring further investigation. Conclusion Marburg I polymorphism of FSAP may not be associated with cerebral infarction.
10.Influencing factor of serum cortisoi levels in the type 2 diabetic pedigrees
Xiaoli WAN ; Wei REN ; Suhua ZHANG ; Song LIANG ; Lilin GONG ; Xiaosu BAI ; Ruizhi ZHENG ; Maorong WANG
Chinese Journal of Endocrinology and Metabolism 2008;24(5):523-524
Serum cortisol levels during oral glucose tolerance test (OGTY) were measured in subjects of type 2 diabetic pedigrees. The results showed that cortisol levels during OGTF were higher in type 2 diabetic patients than those in non-diabetic first-degree relatives and normal controls. Fasting cortisol level was positively correlated with fasting plasma glucose level in type 2 diabetic pedigree members. These results suggest that the dysregulation of hypothalamic-pituitary-adrenal axis may coexist in type 2 diabetic patients.