Objective To investigate the safety and feasibility of laparoscopic cholecystectomy (LC) in the treatment of portal hypertension conplicated by gallstones.Methods A retrospective analysis was performed for the clinical data of 96 patients with portal hypertension complicated by gallstones who underwent surgical treatment in The Fourth People's Hospital of Zigong from January 2012 to June 2016.These patients were divided into laparoscopic group with 50 patients who underwent LC and open group with 46 patients who underwent open cholecystectomy.The surgical conditions and changes in liver function after surgery were compared between the two groups.An analysis of variance with repeated measures was used for comparison of continuous data between multiple groups,and the least significant difference t-test was used for comparison between two groups;the t-test was used for comparison between two groups.The chi-square test was used for comparison of categorical data between groups.Results No deaths occurred during the perioperative period and no patient was converted to open surgery in the laparoscopic group.Compared with the open group,the laparoscopic group had significantly lower time of operation,intraoperative blood loss,length of hospital stay,hospital costs,and incidence rate of postoperative complications (t =2.075,7.389,4.839,and 3.809,x2 =4.697,all P ＜0.05).Both groups had increased or reduced serum levels of alanine aminotransferase (ALT),aspartate aminotransferase (AST),total bilirubin (TBil),and albumin (Alb) at 1 day after surgery,which gradually returned to preoperative levels at 3 or 7 day after surgery.The laparoscopic group had significant changes in the serum levels of ALT,AST,and Alb (F =201.85,185.55,and 4.570,all P ＜ 0.01),while the open group had significant changes in the serum levels of ALT,AST,TBil,and Alb (F =597.84,373.10,5.617,and 10.429,all P ＜ 0.01).There were significant increases in the serum levels of ALT and AST 1 day after snrgery in the laparoscopic group and at 1 and 3 days after surgery in the open group (all P ＜0.05),and at 1 and 3 days after surgery,the laparoscopic group had significantly lower serum levels of ALT and AST than the open group (t =12.537,18.370,9.548 and 15.118,both P ＜0.01).At 1 day after surgery,the open group had a significant increase in the serum level of TBil (P ＜ 0.05) and a higher serum level of TBil than the laparoscopic group (t =2.547,P =0.013).There were significant reductions in the serum level of Alb at 1 day after surgery in the laparoscopic group and at 1 and 3 days after surgery in the open groups (all P ＜ 0.05),and at 1 day after surgery,the laparoscopic group had a significantly higher serum level of Alb than the open group (t =2.291,P =0.024).Conclusion LC is safe and feasible in the treatment of portal hypertension complicated by gallstones,and compared with open cholecystectomy,it has the advantages of a minimally invasive surgery and causes less liver damage.

OBJECTIVE: To determine the content of benzhydramine hydrochloride in Xiaoyan Zhentong ointment by RP-HPLC.METHODS: The determination was performed on Zorbax SB-CN column.Acetonitrile-water-triethylamine(51∶49∶0.5,adjusted to pH 6.5 with acetic acid glacial) was used as mobile phase at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 258 nm and the column temperature was set at 30 ℃.RESULTS: The linear range of benzhydramine hydrochloride was 0.1～4.1 ?g(r=0.999 9) with an average recovery rate at 98.4%(RSD=0.44%).CONCLUSION: The method is accurate,sensitive,reliable and applicable for the determination of the content of benzhydramine hydrochloride in Xiaoyan Zhentong ointment.

BACKGROUND: Studies demonstrated that small intestinal submucosa (SIS) had no immunogenicity, which can not lead to rejection following transplantation, thus, this is an ideal skin substitutes for natural skin.OBJECTIVE: Basic fibroblast growth factor (bFGF) gene was transfected into bone marrow mesenchymal stem cells (BMSCs),and combined the cells with SIS to construct tissue-engineered skin.METHODS: BMSCs were obtained from Japanese big-earad rabbits, and in vitro cultured. Then the subculturad BMSCs were transfected by pCDNA3.1 plasmid, followed by incubation on swine SIS to construct the tissue-engineered skin. The growth of cells and phenotype of BMSCs were detected by flow cytometry. In addition, the result of transfecting BMSCs with pCDNA-bFGF vector was measured by Western blot, and the structure of tissue-engineered skin was observed.RESULTS AND CONCLUSION: After passaged, BMSCs were grown quickly with Iong-fusiform shape. The cells were positive expressed CD90 and CD44, but negative expressed CD45. bFGF had been transfected into BMSCs, and stable expressed. The transfected BMSCs grew well in SIS. By this method, tissue-engineered skin can be constructed in vitro.

Objective To compare the curative effect of anatomical plate and locking plate in treatment of calcaneous intraarticular fracture. Methods 67 petiants with calcaneous intraarticular fracture were randomly divided into anatomical plate group (n=33) and locking plate group (n=34) . The Bo..hler angle, the Gissane angle, the length of calcaneal axis, the width and height of calcaneous and the Maryland grade were compared at 1 week and 6 month after operation. Results (1) week after operation, the Bo..hler angle, the Gissane angle, the height and width of calcaneous, the length of calcaneal axis, the Maryland grade had no significant difference between 2 groups . 6 months after operation, the Bo..hler angle, the Gissane angle, the height of calcaneous had significant differe nce between 2 groups. There was no significant difference in the length of calcaneal axis and the grade of Maryland between 2 groups. Conclusions The locking plate group is better than anatomical plate group in major anatomical measure indicators in 6 months follow up. The therapy of locking plate is worth of clinical promotion.

Objective To evaluate the incidence, prognosis and influencing factors of lower cranial nerve palsy after microvascular decompression (MVD) in patients with hemifacial spasm (HFS). Methods Clinical data of 1033 patients with HFS, admitted to our hospital from May 2014 to August 2016, were retrospectively analyzed. The incidence of lower cranial nerve palsy after procedure and prognosis of these patients were summarized; and influencing factors were analyzed by statistical method. Results In 1033 patients with HFS, 10 patients (0.97％) had lower cranial nerve palsy after procedure: 6 patients suffered hoarseness, 2 patients suffered dysphasia, and 2 patients were with both symptoms; the symptoms in 6 patients completely recovered within one month, 2 patients within one-3 months, and 2 patients within 3-6 months. No permanent low cranial nerve palsy occurred. There were no statistical significances in age, gender, clinical course, sides, or the types of offending vessels between the lower cranial nerve palsy patients (n=10) and the non-palsy patients (n=1023)(P>0.05). Conclusion Lower cranial nerve palsy is a rare complication after MVD, and type of responsible vessels is not a influencing factor in this complication

Minimally invasive surgery experienced a rapid development in the past thirty years, of which the laparoscopy has been widely used in gastrointestinal surgery. Lymph node dissection is one of the difficulties of laparoscopic radical resection of gastric cancer. The lymph node dissection along the left gastroepiploic vessels is a difficult part, which usually causes bleeding and splenic injury. This article mainly introduce the indications, contraindications, surgical preparations, the difficulties and techniques during the lymph nodes dissection when the operator stood on the left side of the patients, and perioperative complications of lymph nodes dissection along the left gastroepiploic vessels.

ObjectiveTo investigate the short-term efficacy of orthopedic elastic bandages on gait symmetry and walking ability in children with spastic hemiplegic cerebral palsy. MethodsFrom June, 2020 to June, 2023, 31 children with spastic hemiplegic cerebral palsy from Beijing Bo'ai Hospital were randomly divided into control group (n = 15) and experimental group (n = 16). Both groups received routine rehabilitation, while the control group received routine walking training, and the experimental group wore an orthopedic elastic bandage for walking training, for four weeks. The indexes of gait symmetry of foot deviation angle ratio (affected/healthy), step length ratio (affected/healthy), gait line ratio (affected/healthy) and standing stage ratio (affected percentage/healthy percentage) were calculated before and after training, and they were measured step width and the optional and maximum walking speed of 10-meter walk test (10MWT). ResultsOne case dropped off in the experimental group. After training, the foot deviation ratio, step length ratio, gait line ratio, and standing stage ratio improved in both groups (|t| > 2.434, P < 0.05), and they were better in the experimental group than in the control group (|t| > 2.230, P < 0.05); while the optional and maximum walking speed of 10MWT improved in both groups (|t| > 9.186, P < 0.001), and they were better in the experimental group than in the control group (|t| > 2.278, P < 0.05). ConclusionWearing orthopedic elastic bandages during rehabilitation can promote the gait symmetry and walking ability of children with spastic hemiplegic cerebral palsy.