Objective To study the selection of the follow-up care and related factors in stroke patients after acute treatment in general hospital. Methods A total of 230 acute stroke patients discharged from January to December, 2015, were reviewed. They were classified with age, modified Rankin Scale (mRS) score, referral options, etc., and the key consideration in the selection were compared. Results There were 125 patients needing referral. Referral increased while mRS worsening (P<0.05). The demand for the continuation of rehabilita-tion was higher in the patients with mRS scores of 0-1 and 2-3 than that of 4-5 (F=4.300, P<0.05), but the latter cared more about medical quality (F=3.464, P<0.05) and hospitalization length (F=3.692, P<0.05). The patients more than 80 years old cared more about medical qual-ity (F=4.630, P<0.05). The patients selecting continuous treatment in our hospital paid more attention on continuation (F=7.590, P<0.001), while those selecting rehabilitation institutions cared more about rehabilitation capacity (F=32.156, P<0.001), and those selecting communi-ty hospitals or nursing home cared more about hospitalization length (F=18.823, P<0.001), easy to visit (F=14.070, P<0.001), etc. Referral information mainly came from the doctors (72.0%), only 28.0% from the patients. Conclusion Severity of disability, age and the prognosis may impact the choice for the following rehabilitation in acute stroke patients. Doctors can do more for reasonable referral recommendations to the patients.

Objective To explore the different methods of decoloration for immunohistochemical HE slides,and their influence on tissue antigen expression.Methods Antigen retrieval solution (EDTA or citric acid solution) was used in immunohistochemistry to decolor HE slides,and was compared with the method of hydrochloric acid solution decoloration in normal temperature.Results Antigen heat retrieval decolors completely.Compared with other methods,it has shorter decoloration time (1 ～ 2min),more than 90％ of completely decoloration rate,the difference was significant(x2 =27.6,23.9,all P ＜ 0.01).Meanwhile,the method dose not damage slides,and the immunohistoehemical results are exact,without background coloring and unusual coloring,antigen expression rate and intensity are high.Conclusion Antigen heat retrieval decoloration not only simplifies the coloration process,but also protects tissue antigen activity better.It is the preferable method of decoloration and protecting antigen resource,which possesses some practical value in the pathology diagnosis.

Objective: To establish the quality standards for Xueyujiangn Capsules(total icariin daidzin Fructus Foeniculi, etc.).Methods:A method for the assay of daidzin by HPLC was described. Results: The average recovery was 101.75% and RSD was 2.5%. Conclusion: The method is simple. This study provides a method for the quality control of Xueyujiangn Capsules.