Objective To study the serum gastrin levels of mothers and neonates via vaginal delivery and cesarean section. Methods The serum gastrin concentrations of 60 women underwent vaginal delivery and elective cesarean section, and the umbilical serum gastrin concentrations of neonates delivered via vagina (20 cases) and cesarean section (22 cases) were measured by radioimmuno assay. Results The serum gastrin concentrations of women in labor(108.23?24.39) ng/L were significantly higher than that of women during cesarean section( P

OBJECTIVE: To prepare Oxymatrine enteric-coated pellets.METHODS: The coating conditions such as the rotation speed,the coating temperature as well as the pressure of spray gun were optimized through single factor experiment;the properties of different coating solutions of resins were compared to optimize the coating material.The enteric-coated pellets were prepared by bed coating technology.The coating effect was evaluated by the dissolution in vitro. RESULTS: The optimal coating conditions were as follows: the rotation speed of the coating pan was 50 r?min-1,the coating temperature was 30 ℃,the pressure of spray gun was 0.10 MPa;the coating material was polyacrylic resin(Huzhou Ⅲ).The in vitro dissolution of the enteric-coated pellets prepared by the bed coating technology met the requirements specified in the Chinese Pharmacopeia(2005 edition).CONCLUSION: By using the bed coating technology,the enteric-coated pellets with good dissolution in vitro can be obtained.

After the problems in military postgraduate information literacy education were analyzed, the construction of a systematic and coherent information literacy education course system for military postgraduates in close combination with their subjects was proposed by setting up multidimensional education target, designing three-stage foundation-subject-innovation course, combining traditional literature retrieval course and embedded professional course, combining case teaching, heuristic discussion and innovative talent information literacy training approach, by infiltrating the physical and virtual space both in and outside university, and by participating in the study and research activities of postgraduates.

Arsenic and its species in dry seafood products(DSPs) Kelp, Sargassum fusiform, Laver, Enteromorpha prolifera, were systematically determined by high performance liquid chromatography-inductively coupled plasma mass spectrometry(HPLC-ICP-MS). It was found that the of arsenic species in botanic DSPs are three unknown arsenicals(U_2, U_3 and U_4), which are speculated as arsenosugars and take approximate 63.5% 92.7% of all arsenic species detected. These arsenosugars were indentified as dimethylarsinic(DMA)-glycerol ribose, DMA-phosphate ribose and DMA-sulfate ribose by their accuracy molecular weight 329.0599, 483.0738 and 409.0162, using direct confirmatory of HPLC-TOF-MS. The quantitative analysis of these arsenic species shows that although the total arsenic exceeds the National Standard value, most of the marine plants DSPs are safe to eat. The main component in most marine plants is DMA-sulfate ribose(51.1%-80.3%), while the main component of Kelp is DMA-phosphate ribose(48.9%). The sum of those three arsenosugars was 63.5%-92.7% of peak areas in all arsenic species detected.

SuperparamagneticSiO2@Fe3O4microspherewithcore-shellstructurewaspreparedbythe method of hydro-thermal and St?ber method, and modified by (3-aminopropyl) triethoxysilane ( APTES) . The obtained amino-Fe3 O4@SiO2 microsphere with controlled surface charge properties was characterized by SEM and TEM, and the surface electric property of amino-microsphere was investigated by Zeta potential measurement. The microsphere was used to extract DNA from human blood, and the solid phase extraction method by microspheres was developed. The mechanism of action between Aminated-Fe3 O4@SiO2 microsphere and DNA was explored, and the gel electrophoresis and PCR test were done on the extraction product. The result showed that genomic DNA with high purity was successfully extracted from whole blood by using amino-Fe3 O4@SiO2 microsphere, with the extraction rate about 70%, the saturated adsorption capacity for DNA was about 40 ng/μg, and the elution could be directly used for further bio-analysis.

Data management service abroad provided by subject librarians in biomedical field for scientific researchers, and the model, contents and efficiency of subject librarians-provided biomedical research data management service in NIH were analyzed according to the need for scientific research data management service in biomedical researchers . The enlightenments of biomedical research data management service abroad on the subject service in our library were elaborated .

AIM To investigate the role of Trp3 in the Ca 2+ influx induced by ? 1B AR in HEK293 cells and the effect of tyrosine kinase on it. METHODS With lipofect AMINE2000 reagent, hTrp3 cDNA was transfected to HEK293 cells and ? 1B HEK293 cells respectively. The expression of Trp3 was examined by Western blot. With Fura 2/AM spectrophoto fluorometry, Ca 2+ influx was determined. RESULTS HTrp3 was expressed endogenously in HEK293 cells, and the expression increased in hTrp3 transfected cells. Compared with untransfected cells, transfection of hTrp3 cDNA increased Ca 2+ influx induced by ? 1B AR ( P 0 05). 5～30 ?mol?L -1 genistein inhibited Ca 2+ influx induced by ? 1B AR in hTrp3 cDNA transfected cells and the maximum inhibitory rate was (75 2?12 6)% . CONCLUSION Transfection of hTrp3 cDNA increased Ca 2+ influx induced by ? 1B AR in HEK293 cells. This process was regulated by tyrosine kinase.

An online enrichment-separation system of C18 and inductively coupled plasma-mass spectrometry (ICP/MS) with flow injection （C18-FI-ICP/MS） was established for the determination of trace heavy metals in seawater. The optimized experimental parameters including pH，C18 column，concentration of methol in elute and the velocity of elute were investigated. This system was applied to the online preconcentration , separation and determination of trace heave metals in seawater samples successfully. The detection limit　(3σ) for Cd, Pb, Co, Ni and Zn was 0.03, 0.09, 0.1, 0.1, 0.3 μg/L respectively. The RSD of Cd, Pb, Co, Ni, and Zn for six measurements of seawater was 6.8%，3.4%，1.3%，2.6%，0.5%，respectively. The accuracy was verified with spiked recovery test. The recovery for five elements was 91.3%，95.1%，100.4%，100.3%，95.2%, respectively.

Objective To evaluate the emotions and quality of life in the patients with thyroid-associated ophthalmopathy (TAO) .Methods 38 TAO patients with normal thyroid function and 34 healthy subjects were recruited .The patients with TAO were further divided into the mild and moderate-severe group by EUGOGO criteria .The depressive and anxious status and quality of life of all subjects were assessed by the rating scales .Results The incidence rate of anxiety and depression in the patients with TAO was 36 .8% and 28 .9% separately ,which were higher than 8 .8% and 5 .9% in the controls(P<0 .05) ,but the quality of life scores in TAO patients were lower than those in the controls (P<0 .05) .The subgroup analysis indicated that the incidence rate of anxiety and depression in female TAO patients was 50 .0% and 45 .5% separately ,which were higher than that in male TAO pa-tients 18 .8% vs .6 .3% (P<0 .05) ,but the quality of life scores in female TAO patients were lower than those in male TAO pa-tients(P<0 .05) .Compared with the mild-moderate group ,an obviously higher incidence rate of anxiety (21 .7% vs .60 .0% ,P<0 .05) and depression(8 .7% vs .60 .0% ,P< 0 .05) were observed in the severe group with the lower quality of life scores (P<0 .05) .Conclusion (1) TAO affects the emotions and the quality of life of TAO patients .(2) TAO patients have a statistically higher incidence rate of anxiety and depression and a lower quality of life ;this phenomenon is more obvious in female patients .(3) The patients with severe TAO are more likely to have anxiety and depression ,as well as a lower quality of life .

Objective To investigate the role of histone lysine methyltransferase G9a in sevoflurane-induced cognitive impairment in the developing brain of neonatal rats.Methods Thirty-six 5-day-old male SD rats were randomly divided into 3 groups (n =12):control group,sevoflurane group and Bix01294 (the inhibitor of histone lysine methyltransferase G9a)group.The rats in the sevoflurane group and the Bix01294 group received 3% sevoflurane anesthesia for 2 hours once a day at postnatal 5-7 days (P5-P7 ).The rats in the Bix01294 group received Bix01294 (10 mg/kg)subcu-taneously at 1 5 min before anesthesia,and the rats in the control group and sevoflurane group received normal saline for injection (0.1 ml)at the same time.The open-field test and fear condition-ing test were performed at P3 5 and P3 9-P41 ,respectively.The tissues of hippocampus were collected at P42 to measure the levels of G9a,histone H3 lysine 9 dimethylation (H3K9me 2 )and synapsin 1. Results Compared with the control group,the percentage of freezing time of sevoflurane group was significantly decreased in the contextual fear condition test,while the levels of G9a and H3K9me 2 were significantly increased and the level of synapsin 1 was significantly decreased (P <0.01).How-ever,the percentage of freezing time of Bix01294 group was significantly increased,while the levels of G9a and H3K9me 2 were significantly decreased and the level of synapsin 1 was significantly in-creased compared with the sevoflurane group (P <0.05).There was no difference in the total distance and residence time in the central grid in the open-field test,and the percentage of freezing time in the cued fear condition test among the three groups.Conclusion Histone lysine methyltransferase G9a is involved in the sevoflurane-induced long-term cognitive impairment in developing brain of neonatal rats,which may be associated with the increase of H3K9me 2 and the down-regulation of synapsin 1 in the hippocampus.