There are some prevailing problems in the management team,such as low competency,lack of innovative ideas,lack of pioneering spirit,and serious brain drain,etc. Therefore our college has taken measures to pay close attention to the development of our management team and has made remarkable achievements with the help of assement.

This article analyzed the current factors affecting the clinical teaching quality,proposed some measures,and discussed some aspects such as ideological recognition,implementing rules and regulations,constructing clinical teaching quality control system in order to improve the clinical teaching quality and train the high quality talents.

Objective To build a core competence evaluation index system for new nurses which was scientific and comprehensive. Methods 23 nursing experts was consulted by a two-round Delphi method, and finally determined the index system and the weight of each index. Results The core competence evaluation index system for new nurses consisted of 6 first-level indicator, 20 second-level indicator, 66 third-level indicator. The coefficient and authoritative coefficient were 0.370, 0.84. Conclusions The formation of the core competence evaluation index system for new nurses is scientific and reliable, which can provide objected and quantitative standards for assessment, training and evaluation of new nurses.

AIM:To study the effect of caffeine on the large conductance calcium activated potassium (KCa) channels by patch-clamp technique on smooth muscle cells enzymatically isolated from the porcine coronary artery (PCASMC),and to investigate the effect of ryanodine on KCa being activated by caffeine.METHODS:Using the single channel patch-clamp technique,single PCASMC was isolated by collagenase,the activity of single KCa channel was recorded in porcine coronary artery smooth muscle cells.RESULTS:Caffeine (0.1-10 mmol/L) enhanced the open probability (Po) of KCa channels in a dose-dependent manner in the intracellular side of inside-out patches and its effect was almost completely abolished by washout. Caffeine decreased the mean close time markedly,but had no effect on the amplitude of KCa channels. However,ryanodine (10-40 ?mol/L) decreased Po of KCa channels activated by caffeine in a dose-dependent manner in cell-attached patches. The mean open time also decreased.CONCLUSION:Caffeine directly activates KCa channels of porcine coronary artery smooth muscle cells in inside-out patches,the activity of single KCa channel is inhibited by ryanodine indirectly in cell-attached patches.

AIM: To investigate the changes of current and gene expression of acetylcholine sensitive K~+ channel (K_ ACh ) in chronic human atrial fibrillation (AF) and to evaluate the roles of K_ ACh expression in the occurring and maintenance of AF. METHODS: Acetylcholine sensitive K~+ currents (I_ KACh ) were recorded with the whole-cell patch clamp technique in single atrial myocyte of AF group and normal sinus rhythm group (SR group). The current densities-voltage relations were analyzed. The Kir3.4 mRNA was measured by reverse transcription-polymerase chain reaction. RESULTS: (1) Compared with SR group, acetylcholine sensitive K~+ current densities in AF group were reduced under testing potential between -80 mV and -120 mV . Acetylcholine sensitive K~+ current density was ( -11.665 ?1.027) pA/pF (n=11) in AF group vs ( -19.486 ?0.766) pA/pF (n=11) in SR group at -100 mV testing potential (P

AIM: To study the effect of caffeine on the large conductance calcium activated potassium (K_(Ca)) channels by patch-clamp technique on smooth muscle cells enzymatically isolated from the porcine coronary artery (PCASMC),and to investigate the effect of ryanodine on K_(Ca) being activated by caffeine.METHODS: Using the single channel patch-clamp technique,single PCASMC was isolated by collagenase,the activity of single K_(Ca) channel was recorded in porcine coronary artery smooth muscle cells.RESULTS: Caffeine (0.1-10 mmol/L) enhanced the open probability (Po) of K_(Ca) channels in a dose-dependent manner in the intracellular side of inside-out patches and its effect was almost completely abolished by washout. Caffeine decreased the mean close time markedly,but had no effect on the amplitude of K_(Ca) channels. However,ryanodine (10-40 μmol/L) decreased Po of K_(Ca) channels activated by caffeine in a dose-dependent manner in cell-attached patches. The mean open time also decreased.CONCLUSION: Caffeine directly activates K_(Ca) channels of porcine coronary artery smooth muscle cells in inside-out patches,the activity of single K_(Ca) channel is inhibited by ryanodine indirectly in cell-attached patches.

Objective To investigate the effects of propofol on the spontaneous transient outward K+ currents in mouse cerebral arterial smooth muscle cells.Methods Kunming mice of both sexes,weighing 18-22 g,were used in this study.Vascular smooth muscle cells were freshly isolated from cerebral arteries in two steps.Five cells were chosen and studied.When holding potential was - 30 mV,spontaneous transient outward K+ currents were recorded before and after the application of 56 μmol/L propofol by perforated whole-cell patch-clamp technique.The amplitude,frequency,area under the curve and half time width of spontaneous transient outward K+ currents were analyzed.Results Propofol 56 μmol/L significantly increased the amplitude,frequency and area under the curve of spontaneous transient outward K+ currents.There was no significant change in the half time width of spontaneous transient outward K+ currents after administration,Conclusion Propofol can activate spontaneous transient outward K+ currents in mouse cerebral arterial smooth muscle cells,and thus induces vascular smooth muscle relaxation.

Objective The aim of this study was to determine the research ability,explore the reasons and point out the direction to improve the research ability of nurses in our hospital.Methods 375 cases of nurses in clinical work in our hospital were chosen with random sampling method to investigate the research ability.Results The variability of research ability score of nurses under investigation was comparatively large.The minimum of scores was 10,the maximum was 113 and the average score was 55.34±25.01.Various scores were all relatively low.The general trend is that the higher the academic and professional titles,the better the research ability.The various scores were independent of administrative rank.Conclusion General research ability of nurses in our hospital was relatively weak.It is necessary to train nurses in our hospital,especially those with lower academic and professional titles to improve the general research ability.

To cultivate laboratory medicine undergraduates with innovation ability , we estab-lished laboratory medicine undergraduates innovation ability training system by formulating flexible teaching plan, reforming teaching methods, setting up diversified practice base, implementing 'eagles plan' and connecting with international content. Satisfactory effect was achieved after taking this measure.

Objecitve:To study the effects of ketamine on the large conductance Ca~(2+)-activated potassium (BK)channels in cultured newborn rat hippocampal neurons. Method:All experiments were carried out on 3-7 days old neurons. Single channel current signals were recorded in symmetrical high K~+ solution(140mM) under cell-attached patch and inside-out patch. Result:(1)In inside-out patch, with the unitary conductance being 176.5?16.5pS,the BK channel activity was markedly activated by internal Ca~(2+) but was blocked by potassium channel blocker (TEA). (2) In cellattached patch,when the concentration of ketamine was 0.01,0.05,0.20mmol/L and membrane potential kept at?60mV,the open probability (Po) decreased from 0.042?0.011 at baseline to 0.027?0.008,0.030?0.015,0.032?0.021 respectively(P0.05). Conclusion:Ketamine has dual effects on the BK channels,the inhibition may be related to its side effects,and the activation to the mechanism in anesthesia.