DNA double strand breaks are potentially lethal DNA lesions induced by ionizing radiation, which can be repaired by homologous recombination and non- homologous end joining. Inhibition of the expression and function of genes in double strand breaks repair pathways using RNA interference and anti- sense techniques could improve the radiosensitivity of cancer cells.

Hypoxia is an inherent feature of the majority of solid tumors,which can increase the resistance of tumor cells to radiotherapy and chemotherapy,promote tumor angiogenesis and lead to poor prognosis.Therefore,targeting the hypoxic tumor cells has become a spot in cancer therapy.Bioreduction drugs can be activated by a specific reduction to become cytotoxic metabolites,thus killing hypoxic tumor cells,while small molecular targeting inhibitors can selectively act on the key point of hypoxia pathway.They have paved a new way for hypoxia targeted therapy.

Stereotactic body radiation therapy (SBRT) has been increasingly used in the treatment of early-stage NSCLC,especially for patients who cannot undergo surgery.Compared with other imaging modalities,PET/CT has higher sensitivity and specificity in diagnosis and staging of NSCLC as well as higher prediction value for prognosis.The progress of 18F-FDG PET/CT in evaluation of the therapeutic response and prognosis after SBRT for early-stage NSCLC is summarized in this review.

Objective: To examine the effect of conjugated linoleic acid (CLA) on lipid metabolism and genes expression of fatty acid transport protein(FATP)and acyl-CoA synthase(ACS) in white adipose tissue of rats. Method: Male Wistar rats were randomly divided into control group, high-fat group and high fat +CLA group (0.75,1.50,3.00 g/100g bw). By reverse transcription polymerse chain reaction (RT-PCR) technique the expression levels of FATP, ACS and peroxisome proliferator-activated receptor-?(PPAR?) mRNA were detected. Results: Serum FFA,insulin and glucose levels of rats fed with high fat diet were higher than those of control group. Supplement of CLA can decrease serum FFA, insulin and glucose levels of obese rats, and increase expression of FATP,ACS and PPAR?mRNA in adipose tissue of obese rats. Conclusion: CLA can improve insulin resistance of obese rat and increase expression of FATP,ACS mRNA, possibly acting through activating PPAR?.

Objective To explore the effect of 10％ and 25％ oral glucose on neonatal pain during blood sampling through radial artery.Methods 90 newborns were randomly divided into group A,B and C,each group with 30 cases.2 min before the blood sampling,group A was offered 2ml 10％ oral glucose,group B was offered 2ml 25％ oral glucose,and group C was offered 2ml plain boiled water by oral intake.Pain was evaluated based on Neonatal Facial Coding System (NFCS),Neonatal Infant Pain Scale(NIPS),raise of heart rate and crying cases.Results The scores of NIPS during puncture,the scores of NFCS and NIPS after puncture,the raise of heart rate and crying cases were significantly different between three groups.Group B were lower than group A and group C,while differences between group A and group C had no statistical significance.Conclusions During blood sampling through radial artery,offering newboms 2ml 25％ oral glucose can reduce the pain,while 2ml 10％ oral glucose was ineffective.

Three bacteria of decomposing lecithin and 4 bacteria of dissolving aptite were incubated for 4 weeks with sand media respectively. Phosphorus in the sand was extracted with distilled water and measured by different methods. It was found that the bacteria have a quite different ability to release P from the materials. Part of the P released became organic phosphorus compounds in microbial tissue. However, a large amount of the P was reserved in microbial cells in a form of phosphates. The direct measurement of P in the extract by molybdenum blue method would underestimate the capacity of the bacteria to release P from the materials. The correct approach was that the sand was fumigated with chloroform and then digested with acid before the measurement by molybdenum blue method.

Objective To investigate the influential factors of brachial-ankle artery pulse wave velocity (baPWV) in healthy individuals.Methods Total 716 healthy subjects including 503 males and 213 females,who underwent health check up in 2010,were enrolled in the study Bilateral baPWV was examined in all subjects and multiple factor linear regression was applied to evaluate the correlations of baPWV with sex,age,body mass index,arterial blood pressure,ankle-brachial index and biochemical variables.Results The mean baPWV of 716 healthy subjecs was ( 1224 ± 162) cm/s,and there was significant difference between men and women [ ( 1257 ± 159) cm/s vs.( 1144 ± 143) cm/s].There was a significant linear correlation of baPWV with sex,age,mean arterial blood pressure and plasma homocysteine concentration ; and the linear regression equation was:mean PWV =6.635 × age + 7.285 × mean arterial blood pressure + 2.682 × homocysteine concentration - 64.334 × sex + 347.500 ( sex:man =0,woman =1 ).Conclusion Age,sex,mean arterial blood pressure and plasma homocysteine concentration are independent influential factors of baPWV.

Objective To understand the experience of stroke patients during the period of com-munity rehabilitation by analysis of the in-depth feeling of them in order to supply reference for communi ty rehabilitation nursing. Methods According to the possible problems of stroke patients including in-nermost feelings, difficulties during community rehabilitation, and the expectation of getting solution, by the instruction of Van Manen's hermeneutic phenomenological methods and adoption of in- depth inter-view, the data of 11 participants were collected and analyzed deeply. Results By using the principle of reflection, comparison and induction six mutually exclusive themes emerged, which were tided: sadness and dread, frustration and useless, fate, unfair and punishment, infeh disguise, self-balance and aspiration of the professional guidance. Conclusions The professional instruction for stroke patients and their families is impending during the period of community rehabilitation.

Objective To observe the survival of human umbilical cord derived mesenchymal stem cells (hUC-MSCs) after injection into the vitreous of rabbits,and the animal safety under those procedures.Methods Twenty-seven pigmented rabbits were randomly divided into 3 groups (intravitreal injection 1 week group,2 weeks group and 4 weeks group),each with 9 rabbits.For each animal the right eye was the experimental eye receiving hUC-MSCs injection,while the left eye was the control eye receiving cuhure medium.The rabbit eyes were examined by slit-lamp microscope,indirect ophthalmoscopy,fundus photography,fundus fluorescence angiography(FFA)and Tono-pen tonometer before and after injection.hUC-MSCs were labeled by CM-Dil in vitro,and their survival status was measured by eonfocal fluorescence microscopy,light microscope and transmission electron microscope at 4 weeks after injection.Results Four weeks after injection,a large number of the hUC-MSCs were still alive in the vitreous cavity.The overall condition of those rabbits was good.The anterior segment and retina of experimental eyes were normal,without hyperfluorescence,hypofluorescenee and leakage in the retina at 1,2 and 4 weeks after injection.There was no significant difference on lOP before and after injection at different time points (P>0.05),and no obvious changes at cornea,anterior chamber angle,lens,retinal structure by.light microscope and transmission electron microscope examination.Conclusion hUC-MSCs can survive in the rabbit vitreous for four weeks;intravitreal injection of hUC-MSCs was safe and feasible.

Objective:To explore diagnostic value of nocturnal ST-T changes in 24h dynamic electrocardiogram (DCG)for coronary heart disease (CHD)and its clinical significance.Methods:A total of 103 cases,who showed ST-T changes in 24h DCG,received selective coronary angiography (CAG).Among them,the 56 patients with in-termittent nighttime significant ST-T changes were regarded as research group,while the other 47 patients with per-sistent ST-T changes were treated as control group.CAG results were compared and analyzed between two groups. Results:Compared with control group,there were significant rise in CAG positive rate (31.9% vs.67.9%),inci-dence rates of dyspnea and chest pain (27.7% vs.66.1%),hypertension (48.9% vs.71.4%),hyperlipidemia (31.9% vs.42.9%)and diabetes mellitus (17.0% vs.46.4%),percentages of lesions in left anterior descending artery (LAD,21.3% vs.57.1%),left circumflex coronary artery (LCX,14.8% vs.37.5%)and right coronary artery (RCA,12.8% vs.35.7%)in research group,P <0.05 or <0.01. Conclusion:Nocturnal ST-T signifieantly changes in 24h DCG,it possesses more diagnostic value for CHD,which can be regarded as a more sensitive index diagnosing myocardial ischemia.