Objective To investigate the effect of Tanreqing injection on pulmonary function, blood gas index and clinical symptoms in patients with chronic obstructive pulmonary disease.Methods 90 cases of chronic obstructive pulmonary disease patients from January 2010 to December 2013 in Xinjiang Tuha Petroleum Hospital were selected as the research object.They were randomly divided into the observation group and the control group, 45 cases in each group.The control group received routine western medicine, on the basis of this, the patients in the observation group were treated with Tanreqing injection.Before treatment and 15 days after treatment, compared the two groups of patients with pulmonary function , blood gas index and the improvement of clinical symptoms.Results Before treatment, there were no significant differences between the two groups of patients with FEV1 , FVC, FEV1/FVC and other pulmonary function index, PaO2 , PaCO2 , SaO2 and other blood gas index and the scores of clinical symptoms.15 days after treatment, the two groups of patients with FEV1 , FVC, FEV1/FVC and other indicators of pulmonary function significantly increased, while its PaO2 , SaO2 levels also increased significantly, the level of PaCO2 in the two groups was decreased, and the clinical symptom scores were significantly decreased.However, the improvement rates of the observation group were significantly higher than that of the control group, the differences between the groups were statistically significant (P<0.05).Conclusion On the basis of conventional western medicine treatment, add Tanreqing injection to treat chronic obstructive pulmonary disease, can significantly improve the pulmonary function and blood gas index level, the clinical symptoms have been effectively relieved.

Objective To investigate the influence of indoleamine 2, 3-dioxygenase (IDO) (activity) from mesenchymal stem cells (MSCs) on the allogeneic T-lymphocytes responses.Methods MSCs were isolated and cultured from human bone marrow. Selected surface antigens of MSCs were detected by flow cytometry and their morphologic characteristics were determined by microscopy. In MSCs induced by ?-interferon (IFN-?) at different concentrations (0, 20, 50, 100, 200 U/ml), IDO mRNA expression and IDO activity were detected. MSCs Induced by IFN-? at the concentration of 200 U/ml were plated in dishes and then mixed lymphocyte reaction (MLR) cultures were set up. T- (lymphocytes) proliferation was determined by MTT assays and IDO activity was determined by high-performance liquid chromatography (HPLC).Results IFN-? could stimulate the IDO mRNA expression and IDO activity in MSCs in a dose-dependent manner. IDO enzyme activity in MSCs inhibited T-lymphocytes proliferation of MLR cultures.Conclusion MSCs could suppress T-lymphocyte responses via IDO enzyme activity in vitro.

AIM: To study the biology characteristics of mesenthymal stem cells(MSCs) derived from chronic myelogenous leukemia(CML) and normal adult bone marrow.METHODS: Mononuclear cells from chronic myelogenous leukemia(n=19) and normal adult(n=8) bone marrow were obtained,cultured in expanded medium with low serum concentration.Cell morphology,cell cycle,immunophenotype and in vitro differentiation capacity were investigated.The differentiations of osteocytes and adipocytes were detected by von Kossa staining and Oilred O staining.The chimeric oncogene BCR/ABL and Ph chromosome,two hall marks of CML,were detected in CML derived MSCs,normal adult MSCs,CML derived hematopoietic cells and K562 cells.RESULTS: CML and normal adult derived MSCs showed similar characteristics in cell morphology,phenotype and growth pattern.A typital fibrablast like morphology was observed.Under suitable conditions,CML and normal adult MSCs had the similar ability to differentiate into adipocytes and osteoblasts in vitro.Moreover,CML and normal adult MSCs did not express BCR/ABL gene products and Ph chromosome was not observed.CONCLUSIONS: We isolated and cultured a population of cells with characteristics of multipotent stem cells from CML bone marrow.There were similar biologic characteristics and differentiation ability between normal adult and CML bone marrow-derived MSCs.

AIM: To investigate the isolation,purification,expansion and multilineage differentiation of mesenchymal stem cells(MSCs) derived from human umbilical cord vein in vitro.METHODS: By 1% collagenase Ⅱ digestion,endothelial cells were isolated from human umbilical cord vein and cultured in IMDM medium.The morphology of the cells was observed by Wright's staining and electron microscope.Cell cycle and immunophenotype were investigated by flow cytometry.Assays of adipogenic and osteogenic differentiation were performed in vitro.von Kossa staining,Oil Red O staining and mRNA expression of osteopontin and lipoprotein lipase were studied in the induced cells.RESULTS: The cells from the cord vein displayed a fibroblast-like morphology adhering to the culture plate.FACS showed that the cells expressed several MSCs-related antigens such as CD29,CD44 and CD105,while CD13,CD31,CD45,CD34,and HLA-DR were negative.Adipocyte and osteocyte differentiation were induced successfully.CONCLUSION: The morphology,growth characteristics,immunophenotype and pluripotentiality of the MSCs from human umbilical cord vein are similar to the MSCs from bone marrow(BM).They could potentially be an excellent source of MSCs for experiments and clinics.

AIM: To investigate the influence of mesenchymal stem cells (MSCs) expressing indoleamine 2, 3-dioxygenase (IDO) activity on the allogeneic T-lymphocyte responses.METHODS: MSCs were isolated and cultured from human bone marrow. Selected surface markers of MSCs were detected by flow cytometry and their morphologic characteristics were determined by microscopy. The pluripotentiality of MSCs was also studied. IDO mRNA and IDO protein expressions in MSCs induced by γ-interferon (IFN-γ) at the concentration of 2×105 U/L were detected. MSCs induced by IFN-γ at the concentration of 2×105 U/L were plated in dishes and then mixed lymphocyte reaction (MLR) cultures were set up. T-lymphocytes proliferation was determined by MTT assays and IDO activity was measured by high-performance liquid chromatography (HPLC). RESULTS: IFN-γ could stimulate IDO mRNA and IDO protein expressions in MSCs. IDO enzyme activity in induced MSCs inhibited T-lymphocyte proliferation of MLR cultures. CONCLUSION: MSCs could suppress T-lymphocyte responses via IDO enzyme activity in vitro.

The immune mechanism of Graves' diseases (GD) and the roles of regulator T cells were investigated. In 32 patients with GD (GD group) and 20 healthy volunteers (control group), flow cytometry was used to detect the proportion of CD4+CD25+ cells, MACS to isolate CD4+ CD25+ cells, RT-PCR to assay the expression of FOXP3, and ELISA to test the level of IL-10, respectively. It was found that there was no significant change in the proportion of CD4+CD25+ T cells between GD group and control group (P>0.05), while secretion of IL-10 and expression of FOXP3 in GD group were lower than control group (P<0.01 and P<0.05, respectively). In conclusion, though the proportion of regulatory T cells of peripheral blood lymphocytes in the patients with GD, the functions of them were significantly weakened, which might be a pathogenic factor in GD.

The immune mechanism of Graves' diseases (GD) and the roles of regulator T cells were investigated. In 32 patients with GD (GD group) and 20 healthy volunteers (control group), flow cytometry was used to detect the proportion of CD4+CD25+ cells, MACS to isolate CD4+ CD25+ cells,RT-PCR to assay the expression of FOXP3, and ELISA to test the level of IL-10, respectively. It was found that there was no significant change in the proportion of CD4+CD25+ T cells between GD group and control group (P＞0.05), while secretion of IL-10 and expression of FOXP3 in GD group were lower than control group (P＜0.01 and P＜0.05, respectively). In conclusion, though the proportion of regulatory T cells of peripheral blood lymphocytes in the patients with GD, the functions of them were significantly weakened, which might be a pathogenic factor in GD.

Objective To observe the efficacy and safety of tiopronin supplemented with chemotherapy in treating advanced breast cancer.Methods Sixty patients with advanced breast cancer were randomly divided into two groups:treatment group(n = 28) and control group (n = 32).Two groups were treated the same of NVB + DDP,the treatment group was supplemented with tiopronin,given for 10 days.Efficacy,toxicity in two groups were compared.Results The effective rate in the treatment group and the control group were 46.4% and 46.9% respectively, with no significant difference between the two groups ,P > 0.05.But the improved quality of life of patients in the treatment group was higher than that in the control group, P < 0.05.The rate of adverse reaction in liver function damaged (9.4%)and leucocyte lassitude(46.4%) were apparent lower than those in the control group(31.2% ,81.2% ),with significant difference between the two groups(P < 0.05, P < 0.01).Conclusion Tiopronin supplemented with chemotherapy show apparent effect in decreasing the adverse reaction of chemotherapy,improving the quality of life and not influence efficacy in advanced breast cancer.So tiopronln may act as protective drug for chemotherapy and deserve further testing in the clinic.

Objective To observe the difference of retinal vessel oxygen saturation in glaucoma and normal eyes.Methods A cross sectional study design was performed.Fifty eyes of 30 glaucoma patients (glaucoma group) and 41 eyes of 27 age-and sex-matched healthy subjects (control group) were included.Retinal vessel oxygen saturation was measured with a spectrophotometric retinal oximeter in darkness and visual fields were obtained by Humphrey filed analyzer.The glaucoma eyes were divided into two groups:mean defect (MD) ＜6 dB (28 eyes) and MD≥6 dB (22 eyes) according to mean defect of visual field.Results Retinal arteriolar oxygen saturation values in glaucoma group and control group were (94.52 ±6.51) ％ and (93.47±6.30) ％ respectively.No statistical difference was found in retinal oxygen saturation in arterioles (H =-0.949,P =0.343).Retinal venous oxygen saturation values in glaucoma group and control group were (57.57 ± 7.96)％ and (52.60 ± 7.70) ％ respectively.The retinal venous oxygen saturation values in glaucoma group was higher than that in control group (H=-3.318,P=0.001).The retinal arteriovenous difference in glaucoma group and control group were (36.59± 4.69)％ and (42.41 ±6.73) ％ respectively.The retinal arteriovenous difference in glaucoma group was lower than that in control group (H=-4.148,P＜0.01).The retinal arteriolar oxygen saturation values in glaucoma eyes with MD＜6 dB and MD≥6 dB were (93.38 ± 6.33)％ and (95.71 ± 6.54)％ respectively,with no statistical difference (H=-1.857,P=0.063).Retinal venous oxygen saturation values in glaucoma eyes with MD＜6 dB and MD≥6 dB were (54.83 ± 6.10) ％ and (6 1.07 ± 8.79) ％ respectively.The retinal venous oxygen saturation values in MD≥ 6 dB glaucoma eyes was higher than that in MD＜ 6 dB glaucoma eyes (H =-2.599,P=0.009).The retinal arteriovenous difference in glaucoma eyes with MD＜6 dB and MD≥6 dB were (38.12± 4.34) ％ and (34.64 ± 4.49) ％ respectively.The retinal arteriovenous difference in MD≥6 dB glaucoma eyes was lower than that in MD＜6 dB glaucoma eyes (H=-2.463,P＜0.05).Conclusions Compared with healthy eyes,there is no change in the retinal arteriolar oxygen saturation,but the retinal venous oxygen saturation is higher and the retinal arteriovenous difference is lower.This feature is more obvious in MD≥6 dB glaucoma eyes.

OBJECTIVE:To extract volatile rose oil by the supercritical fluid extraction technology.METHODS:The optimum extraction technology condition was investigated by orthogonal experiment with extraction rate as the evaluation in?dex,and with the pressure,temperature of extraction and the flow of CO 2 as investigation factors(3levels of each were cho?sen).RESULTS:The optimum technology condition was the following:the extraction pressure was25MPa,the temperature was50℃and the flux of CO 2 was600L/h.CONCLUSION:The established method has the following merits:high extraction rate,fast speed,simple technics,pollution-free,pure extraction etc.