This paper recommends the quantitative determination of endotoxin in PVC disposable transfusion set by limulus test for the first time in our country. The data obtained can act as reference material for eontroling the quality of the transfusion set. The p-nitroaniline staining combined with spectrophotometry was used in this study. The precision, sensitivty and repetition rate of this method were satisfactory. We conclude that this method is an ideal one for quantitatively determining endotoxin in PVC transfusion set.

One hundred patients were diagnosed with cervicalspondylotic radiculopathy definitely by symptoms, signs and X-ray examination. The therapeutic method was acupuncture of Jiaji(Ex-B 2) on the affected segment and the upper and lower vertebrae close together. After the arrival of qi, the needles were connected to acupuncture therapeutic instrument. Meanwhile, the numb, heavy and uncomfortable area on the shoulder and upper limb, where something like cord could be touched, was selected as Ashi point. The needle was inserted perpendicularly into the skin on the place that the cord was most obvious and inserted obliquely and deeply along the cord. After 3-4 courses of treatment, 90 cases got marked effectiveness and 10 cases got effectiveness.

Objective To investigate the treatment of proximal femoral nail antirotation (PFNA) in pertrochanteric hip fractures with unstable lateral-wall.Methods Thirty-two cases were included from the April 2009 to May 2011.Recorded operation period,blood loss volume,and complications and evaluated the effect of the methods.Arrange referral at 6 weeks,3 months,6 months,12 months respectively after operation,to check limb function and conventional camera hips,lateral X-ray film.Postoperative functional evaluation using the Harris hip score.Measure the last follow-up neck shaft angle and compare with postoperative's.Results The average operation period was (60-190) min,average was (80 ± 5) min ; blood loss volume was (90-250)ml,average was (120 ± 9) ml ; Average hospital periods was (15 ± 7) days; weight loading time was (6.3 ± 1.2)weeks after surgery and average time of union of fracture was 3.6 months.All patients obtained union of fracture without complications such as varus,internal fixation cutting out.Conclusion PFNA was proved to be an effective way for treating unstable lateral-wall of pertrochanteric hip fractures due to its advantages of stable fixation,minor trauma,less complications.

Objective To study the effect of chemically synthesized Nogo-66 receptor ( NgR ) specific small interfering RNA ( siRNA) on nerve regeneration and function of newborn rats after hypoxic-ischemic brain damage ( HIBD) . Methods A total of 50 HIBD newborn rats were set up. They were randomly assigned OR allocated into siNgR group ( n=20 ) , normal saline ( NS ) group ( n=20 ) and HIBD group ( n=10 ) . The rats of siNgR group were given intraventricular injection of siNgR and transfection reagents ( 10μl ); the rats of NS group were given intraventricular injection of NS and transfection reagents (10μl);and the rats of HIBD group had no intervention. In addition to these three groups, there is another group, sham-operated group ( n=10 ) . The rats of sham-operated group were sham-operated ( common carotid artery was isolated but not ligated) and did not receive hypoxia-ischemia processing and intraventricular injection. Utilize water maze experiment to analyze the rats' escape time. The levels of NgR and GAP-43 protein in rats' brains were measured by immunohistochemistry and image analysis. Results RT-PCR gel electrophoresis results showed that the NgR cDNA stripe of siNgR group was not obvious, but the stripe of NS group was clear. At the same time, the GAPHD cDNA bands of the above two groups were both clear. There were more NgR positive immune reaction products ( brown particles) in NS group than in siNgR group. The number of GAP-43 positive cells by immunohistochemistry in sham-operated group, HIBD group, NS group and siNgR group was (33. 24±1. 32), (20. 14±1. 24), (18. 73±1. 41) and (28. 06±1. 78), respectively. The number of sham-operated group and siNgR group was greater than HIBD group and NS group ( P＜0. 05 ) . There was no statistical significant difference for the number of GAP-43 positive cells between sham-operated group and siNgR group ( P＞0. 05 ) . Water maze experiment results showed that the newborn rats ' average escape time ( s ) of HIBD group ( 58. 1 ± 10. 3,47. 2±10. 1, 42. 5±7. 6) was obviously longer than sham-operated group (34. 2±5. 6, 25. 7±6. 2, 21. 2±8. 1), and the difference was statistically significant (P＜0. 05). However, the average escape time of siNgR group (37. 5±9. 8, 29. 1±9. 8, 27. 2±9. 3) was obviously shorter than HIBD group and NS group (60. 7±5. 2, 49. 1±9. 9, 45. 3±9. 3), (P＜0. 05). Conclusions Chemically synthesized specific siRNA had the potential to interference the expression of NgR in the brain of newborn rats, and to a certain extent, could promote the nerve regeneration and neural functional recovery of rats.

Objective To study the role of bcl 2 gene expression in human prostate. Methods The level of bcl 2 gene mRNA expression were investigated by in situ hybridization in 11 cases of fetal prostates, 27 cases of adult (specimens obtained from autopsy)and 60 cases of hyperplastic prostates. Results In fetal prostates, positive signals were detected in all epithelial cells of the prostate buds, while in adult and hyperplastic specimens, positive signals were predominantly concentrated to the basal cell layer of the epithelium, with little involvement of the secretory epithelial cells. The expression in hyperplastic prostates was significantly higher than in normal ones and that of the basal epithelial cells was significantly higher than the secretory ones in both the normal prostates and the hyperplastic ones. Conclusions Bcl 2 gene may play an important role in regulating the apoptosis of the basal cells while if plays a secondary role in the secretory cells of the prostate.

Objective To explore the antibacterial activity of the baicalin injection in vitro.Methods The broth doubling dilution method has been adopted.And the minimal inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of baicalin injection on 161 common pathogenic bacteria obtained from clinical practice were determined.Results The MIC range of baicalin against clinically common pathogenic bacteria is 0.1～50mg/ml and the MBC range is 0.2～50mg/ml.The MIC and MBC against staphylococci is remarkably lower than those against other bacteria,with the MIC range of 0.1～6.3mg/ml and MBC range of 0.2～12.5 respectivel.The MIC50,MIC90 are respectively 0.4mg/ml and 0.8mg/ml.Conclusions The baicalin injection possesses various levels of inhibitory effect on manifold pathogenic bacteria,especially the antibacterial effect on the staphylococci.

Objective To report 44 cases of meta-superior position anorectal malformation in different age therapied by posterior sagittal anorectoplasty(PSARP) operation, and to discuss possibility of one-stage anorectoplasty via posterior sagittal approach in neonate. Methods The clinical data of 44 cases meta-superior position anorectal malformation,which were treated by PSARP,were studied retrospectively. Results Of 44 cases, 15 were treated by colon stoma in neonate, then PSARP was executed after 3～7 months. And the rest 29 cases (9 in neonate and 20 in 2 months～9 years) were operated with one-stage anorectoplasty via posterior sagittal approach. Among 21 children followed from post-operration 6 months to 5 years, 18 cases of defecation clinical score were fineness. Conclusion The curative effects of PSARP in neonate to meta-superior position anorectal malformation is satisfactory. If the neonate is in good condition, one-stage anorectoplasty via posterior sagittal approach could be executed in infant, and favourable defecate function could be remain.

Objective To investigate the effects and complications of injection with triamcinolone acetonide acetate and Pingyangmycin on maxillofacial and perineal hemangioma. Methods Local injection with triamcinolone acetonide acetate (TAA) and Pingyangmycin (PYM) was performed to treat infant maxillofacial and perineal hemangiomas: strawberry hemangioma, cavernous hemangioma and mixed hemangioma, in 107 infants admitted to our hospital from 1996 to 2005. If the lesion did not disappear after the first treatment, the injection would be performed again every four to six weeks interval, and the total dose of TAA and PYM could not be more than 100 mg and 40 mg respectively. The injection treatment was repeated 4 times for one course and the therapeutic effects on maxillofacial and perineal hemangioma was evaluated. Results All cases were followed up for one year after local injection of TAA and PYM. The cure rate was 87.8%, the effective rate was 9.3%, and the ineffective rate was 2.8%. Conclusion The injection of TAA and PYM is a reliable and less side effect therapeutic method, and valuable for infant maxillofacial and perineal hemangioma.

Objective To investigate the effects of DLXNP on hemorrheology and bulbar conjunctiva microcirculation (BCM) in rabbit and to explore its effects of activating the blood and eliminating the stagnant. Methods The blood stagnant syndrome model was reproduced by injecting dextran 500 into the marginal ear vein. The BCM and the hemorrheology properties were detected and compared. Results Compared with the normal group, the model group's indexes of whole blood relative and whole blood reducing viscosity improved (P

Objective To observe the influence of Xuefuzhuyutang and Erchentang on bulbar conjunctival microcirculation in experimental atherosclerosis rabbits and distinguish the differences of the two recipes. Methods 28 rabbits were randomly divided into 4 groups (control group, hypercholesterol group, Erchentang group, Xuefuzhuyutang group). The models were developed by fed high cholesterol diet, the changes of bulbar conjunctival microcirculation were detected at 10 weeks. Results Xuefuzhuyutang decreased the score values of the capsular dilatation, the diameter of microvein, the red blood cell aggregation (P