Objective To observe the variations of the functional states of T helper cells(Th) in peripheral blood of children with mycoplasma pneumoniae pneumonia(MPP) in acute phase,and to explore their roles in the pathogenesis of MPP.Methods Peripheral blood mononuclear cells(PBMC) of 40 MPP children were stimulated with PMA and ionomycin and cultured in vitro for 48 hours.The levels of IL-4,IFN-?,and TGF-?1 in the supernatants of PBMC were detected by ELISA.Results The levels of IFN-? and the ratio of IFN-?/IL-4 of MPP children in acute phase were significantly lower than those of the controls(P0.05).Conclusions The function of Th2 relatively prevails over Th1 in MPP children.The protective or regulative function of Th3 in vivo increases,but the protective regulation of TGF-?1 is insufficient,so the imbalance of Th1/Th2 can not be corrected.The variation of the functional states of T helper cells in acute phase has no relationship with or without the extrapulmonary damage.

Objective To study changes of telomerase activity in transitional cell carcinoma after intravesical instillation of Serratia Marcescens . Methods TRAP-PCR and TRAP-sliver staining were used to detect telomerase activity in 20 patients with TCC after intravesical instillation of Serratia Marcescens . Results Positive rate of telomerase activity detection in TCC patients after intravesical instillation of Serratia Marcescens was 50%(10/20),significantly lowed than before the intravesical instillation (90%,18/20), P

To sum up and analyze the representative point compatibility theory after Inner Canon of Huangdi to explore t he compatibility rules of point in acupuncture prescription.On base of references and predecessor,s experience,combined with clinical practice,compatibility rules of point can be concluded that zhu-ke-fu-ying points was the elementary compatibility,and shu-mu-qi points should be selected according to syndrome differentiation,thereby forming a traditional and orderly prescription which reflected principle-method-recipe-point.

Objective The purpose of this study was to determine the value of K-ras mutation in DNA extracted from the plasma as clinical indicator of colorectal cancer. Methods Point mutation at codon 12 of K-ras gene was assayed by polymerase chain reaction of sequence-specific primers in DNA extracted from the plasma and tumors from 32 patients with colorectal cancer. The mutation was further confirmed by dideoxy-mediated chain-termination method of DNA sequencing. Results Fourteen patients (44%) had a codon 12 K-ras mutation within their primary tumors and identical mutations were found in the plasma DNA of 13 patients (93%). Mutant DNA was not detected in the plasma specimens of 18 patients whose tumors tested negative for K-ras alterations or in 5 healthy control subjects. Conclusion Preliminary results suggest the detection of K-ras mutations in circulating DNA extracted from the plasma specimens may have some clinical uti- lity in the detection of colorectal cancer.

Objective To study the influence of health behavior intervention on coping style and hope level in lung cancer patients undergoing chemotherapy. Methods 60 lung cancer patients undergoing chemotherapy were divided into the intervention group and the control group with 30 patients in each group. Two groups all had an operation, carried on the normal nursing care and health education. After operation, the intervention group accepted health behavior intervention from the beginning of the first period chemotherapy. After the third period chemotherapy, coping style and hope level of the two groups were measured. Results After intervention, positive coping style "confrontation" had a higher score. Negative coping style such as obviation and acception got lower scores in the intervention group compared with the control group. In addition to "keep an intimate relation(I)",the rest dimensions of hope level showed significant differences as well. Conclusions Applying the related theories of health behavior to build up mental nursing intervention mode has a significant meaning on lung cancer patients undergoing chemotherapy. It can increase patients' positive coping style and reduce negative aspects. At the same time, hope levels of lung cancer patients also have an improvement.

Objective To know the relationship of self-efficacy and quality of life of patients with breast cancer after postoperative chemotherapy, and then carry out reference to clinical nursing cares. Methods Interviewed 115 patients with breast cancer after postoperative chemotherapy by questionnaire to know their conditon about self-efficacy and quality of life, and then analysis the relationship between them. Results The general score of quality of life of patients with breast cancer was 105.62±6.68, which was significant lower than the normal model. There was significant correlation of self-effi-cacy and quality of life of patients with breast cancer. Conchusions Related experts should emphasis the postoperative management of patients with breast cancer, and then promote their self-efflcacy and quality of life.

Objective To investigate the value of contrast-enhanced ultrasound (CEUS) in differential diagnosis of pancreatic pseudocysts,cystadenomas and cystadenocarcinomas,so as to improve diagnostic accuracy of the condition.Methods The results of CEUS and conventional ultrasound for 71 patients with pancreatic cystic lesions were analyzed retrospectively,and compared with the finally obtained surgical-pathological examination to assess the ability of qualitative diagnosis between two methods.Results In 71 pancreatic cystic lesions,there were 32 pancreatic pseudocysts,24 cystadenomas and 15 cystadenocarcinomas.The sensitivity and accuracy of CEUS for pancreatic pseudocysts and cystadenocarcinomas were 84.4％,93.0％ and 86.7％,88.7％,which were higher than those of conventional ultrasound 56.3 ％,80.3 ％ and 53.3 ％,74.6％ (x2 =6.06,4.92,3.97,4.71,all P ＜ 0.05).Conclusion There are some similarities in terms of imaging manifestations among pancreatic pseudocysts,cystadenomas and cystadenocarcinomas.Combining conventional ultrasound with CEUS can help the differential diagnosis of pancreatic cystic lesions.

Objective To review the diagnosis and treatment of pyloric canal polyp in infants and toddlers suffering progressive gastric outlet obstruction.MethodsClinical data of 5 male infants and toddlers,at age of 10 to 26 months(mean 15.6 months)with pyloric canal polyp admitted in our department from January 1993 to February 2009 were analyzed.ResultsOf 5 cases,the starting was acute and severe.the conservative treatment was out of effect and no any assistant examination was helpful during diagnosis,and every case was diagnosed clearly through exploratory laparotomy,cured with polypectomy and classic pyloroplasty.ConclusionCurrently,the exploratory laparotomy is an important way to diagnose pyloric canal polyp in infants and toddlers,while other assistant examination seems useless in this aspect.Polypectomy and classic pyloroplasty should be carried out during exploration,and it is dangerous for their life without correct therapy in time.

Objective To explore an optimal treatment and to study the prognosis related factors of breast cancer patients with local recurrence after mastectomy. Methods From 2002. 7 to 2005. 2, 477female patients with loco-regional recurrence of breast cancer treated in Tianjin Cancer Hospital were analyzed retrospectively. Results In 477 cases, recurrence within 1 year after mastectomy accounted for 26. 2% , recurrence within 2 years accounted for 61. 2%. There were 310 cases with metastasis after local recurrence was diagnosed, accounting for 65.0%. 5-year total survival rate after recurrence is 48.4%.Local control rates varied in subgroups with different recurrence site, clinical subtypes, radiotherapy fields,with or without radiotherapy, surgical resection or excisional biopsy ( P<0. 05 ). There was a statistical difference in distant metastasis rate and 5-year survival rate among subgroups which had different clinical stage of primary tumor, disease-free interval, clinical subtypes or treatment methods ( P<0. 05 ). Simplistic treatment option, late clinical stage of primary tumor and triple-negative breast cancer were the independent factors predicting poor prognosis for recurrent breast cancer ( P<0.05 ). Conclusions Multi-site recurrence and triple-negative breast cancer lead to a poor local control. Local expansion of radiotherapy combined with surgery improves the local control rate. Patients with late clinical stage of primary tumor,recurrence within 2 years, triple-negative breast cancer are likely to have distant metastasis when recurrence is diagnosed. Combined treatment program improves survival rate.

Objective To develop a feasible method for evaluating uncertainty in colony forming units(CFU)on the disinfected skin of nurses of operation room.Methods A nurse of operation room in a hospital involved the study.Ten spots on her disinfected hand skin were selected for evaluation of uncertainty in the acquired data and analysis of reliability of the evaluation methods,according to Technical Norms for Monitoring the Disinfection and Sterilization Quality in Hospitals in Jiangsu Province and JJF1059-1999 Evaluation and Expression of Uncertainty in Measurement.Results The CFU on the disinfected skin of nurse was(4±2)cfu/cm2, which indicated that the disinfection of the nurse's hands was not qualified.Conclusion The evaluation method by our study is reliable for evaluating the uncertainty in CFU on nurse’s disinfected hand skin.It is simple and fitful for the uncertainty evaluation of hospital disinfection effect.