The article expatiated on the process of rabbit respiration regulation and respiratory failure designing experiment.It can fully excitate students'potential and strengthen the teaching effects.It is very important for cultivating students'synthesis diathesis and abilities.

To pay attention to functional virtual laboratory,we can make new networks management,open laboratory and develop students' innovative ability.

In order to adapt to the demand of raising high quality talented person and to strengthen student's comprehensive quality,we have carried on the reform to the medicine function experiment test.After discussions and practices in recent years,we have established a set of relative consummate inspection systems of functional experiment of medicine.The investigation result has demonstrated that the inspection system after reform has a remarkable function on training the students' begins ability,observation ability and the ability to analysis and solve questions.

Objective To explore the antibacterial activity of the baicalin injection in vitro.Methods The broth doubling dilution method has been adopted.And the minimal inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of baicalin injection on 161 common pathogenic bacteria obtained from clinical practice were determined.Results The MIC range of baicalin against clinically common pathogenic bacteria is 0.1～50mg/ml and the MBC range is 0.2～50mg/ml.The MIC and MBC against staphylococci is remarkably lower than those against other bacteria,with the MIC range of 0.1～6.3mg/ml and MBC range of 0.2～12.5 respectivel.The MIC50,MIC90 are respectively 0.4mg/ml and 0.8mg/ml.Conclusions The baicalin injection possesses various levels of inhibitory effect on manifold pathogenic bacteria,especially the antibacterial effect on the staphylococci.

Objective To report 44 cases of meta-superior position anorectal malformation in different age therapied by posterior sagittal anorectoplasty(PSARP) operation, and to discuss possibility of one-stage anorectoplasty via posterior sagittal approach in neonate. Methods The clinical data of 44 cases meta-superior position anorectal malformation,which were treated by PSARP,were studied retrospectively. Results Of 44 cases, 15 were treated by colon stoma in neonate, then PSARP was executed after 3～7 months. And the rest 29 cases (9 in neonate and 20 in 2 months～9 years) were operated with one-stage anorectoplasty via posterior sagittal approach. Among 21 children followed from post-operration 6 months to 5 years, 18 cases of defecation clinical score were fineness. Conclusion The curative effects of PSARP in neonate to meta-superior position anorectal malformation is satisfactory. If the neonate is in good condition, one-stage anorectoplasty via posterior sagittal approach could be executed in infant, and favourable defecate function could be remain.

Objective To investigate the effects and complications of injection with triamcinolone acetonide acetate and Pingyangmycin on maxillofacial and perineal hemangioma. Methods Local injection with triamcinolone acetonide acetate (TAA) and Pingyangmycin (PYM) was performed to treat infant maxillofacial and perineal hemangiomas: strawberry hemangioma, cavernous hemangioma and mixed hemangioma, in 107 infants admitted to our hospital from 1996 to 2005. If the lesion did not disappear after the first treatment, the injection would be performed again every four to six weeks interval, and the total dose of TAA and PYM could not be more than 100 mg and 40 mg respectively. The injection treatment was repeated 4 times for one course and the therapeutic effects on maxillofacial and perineal hemangioma was evaluated. Results All cases were followed up for one year after local injection of TAA and PYM. The cure rate was 87.8%, the effective rate was 9.3%, and the ineffective rate was 2.8%. Conclusion The injection of TAA and PYM is a reliable and less side effect therapeutic method, and valuable for infant maxillofacial and perineal hemangioma.

AIM: To observe the changes of interleukin-6(IL-6), IL-8 and tumor necrosis factor-?(TNF-?) in serum and lung at different time, and the effects of anisodamine (654-2) treatment in rats with oleic acid-induced ARDS. METHODS: The ARDS model induced by intravenous injection of oleic acid in the rat was used and levels of IL-6, IL-8, TNF-? in serum and lung tissue supernatant were measured using enzyme linked immunosorbent assay (ELISA). RESULTS: Levels of serum and lung tissue IL-6, IL-8, TNF-? in oleic acid type ARDS 4 h group were increased significantly. These cytokines in oleic acid type ARDS 8 h group were lower than that of ARDS 4 h group, but serum IL-6, TNF-? and lung tissue IL-6 were still higher than that of control group . In oleic acid type ARDS 16 h group, serum IL-6, TNF-? were lower than that of the ARDS 8 h group and serum TNF-? and lung tissue IL-6 were higher than that of control group. After 654-2 treatment, the levels of serum and lung tissue IL-6, TNF-? were decreased significantly. CONCLUSION: IL-6, IL-8 and TNF-? might play important roles in the oleic acid-induced ARDS in the rat. 654-2 might alleviate ARDS by inhibiting excess production of IL-6 and TNF-?.

Objective To estimate the prevalence of iron deficiency and its influencing factors in 6 to 12-month-old infants,then more effective community nursing intervention of prevention against iron deficiency can be made.Methods Three hundred and thirty-one 6 to 12-month-old infants had been enrolled by convenience sampling method to complete the investigation.Variables associated with iron deficiency in bivariate analysis were evaluated in Logistic regression models.Results The prevalence rates of iron deficiency in 6 to 12-month-old infants was 34.74％.Anemia,age,feeding patterns and zinc deficiency were independently associated with increased risk for iron deficiency.Conclusions Iron deficiency was common and associated with anemia,low age,feeding patterns and zinc deficiency in a certain community in Tianjin.So effective nursing measures must be made to reduce the prevalence rates of iron deficiency in 6 to 12-month-old infants.

Objective To examine the teaching effects of case-based learning in the class of functional experiment. Methods Students (n=139) of five-year clinical medicine specialty were ran-domly divided into control group (n=70) and case group (n=69). The students of control group were taught with traditional teaching method and the case-based learning was adapted in the case group. Namely the clinical cases were led into teaching and the teaching process was completed through case import, problem sets, and the literature review and discussion. The teaching effects were evaluated through questionnaire survey (Analysis by the χ2-test, the test level is 0.05) and the analysis of test scores (Analysis by the t-test, the test level is 0.05). Results The score of the case group was better than the control group in the operation test (P=0.042 0). The total evaluating score and test score were also better in the case group in comparing to the control group (P=0.007 8 or P=0.008 2). As indicated by the questionnaire survey, more than 80%students thought the case teaching method could improve their learning interests and the independent learning abilities. Moreover, the skills of problem analysis and knowledge application were increased through case teaching. Conclusion The teaching effects of case-based learning were superior to the traditional teaching.

Objective To explore the therapeutic effects of Ni-Ti memory alloy embracing fixator on the multiple fracture of ribs and flail chest. Method From January 2006 to December 2009, the effects of operation and long-term follow-up after internal fixation operation with memory alloy embracing fixator in patients with multiple fracture of ribs and flail chest were observed. Results The hospital stay of patients treated with memory alloy embracing fixator was 10.31 ± 3.14 days and pain was significantly reheved after treatment ( P= 0.02) .The rate of post-operative complications was 17.65% such as atelectasis, pulmonary infection, etc. There were fewer longterm complications and less influence to daily activity of life. Conclusions It is a advanced and practical method for patients with the multiple fracture of ribs by using Ni-Ti memory alloy embracing fixator with slighter trauma,simple, safe, reliable, good histocompatibility and fewer post-operative complications.