OBJECTIVE:To establish an HPLC method for the determination of levels of intracellular cholesterol and cholesterol esters in foam cells derived from RAW264.7 murine macrophages so as to obtain a precise measure of foam cells and provide a technical platform for the study of the intracellular cholesterol content change.METHODS:HPLC method was applied to determine the content of cholesterol esters in the foam cells treated with OX-LDL and different concentration of pravastatin.RESULTS:OX-LDL could significantly enhance cellular intake of lipids(P

Objective To explore the clinical efficacy of combined anterior and posterior operation in the treatment of cervical spondylotic myelopathy.Methods 50 patients with cervical spondylotic myelopathy,in accordance with anterior,posterior surgery and combined anterior and posterior operation three different surgical methods,were divided into the treatment group A(21 cases),group B(16 cases) and group C(13 cases).The surgical efficacy was analyzed.Results Before treatment,the JOA score among the three groups had no significant difference [Cgroup (9.3 ± 2.3) points,B group (9.4 ± 1.4) points,A group (9.2 ± 2.2) points].After treatment,the JOA score in group C (14.5 ± 3.4) points was significantly higher than that of group A (12.6 ± 1.2) points (t =3.09,P ＜0.05) and group B (11.3 ± 1.4) points (t =4.62,P ＜ 0.05).The excellent and good rate of C group was 76.9％,which was significantly higher than that in group A 43.8％ (x2 =3.84,P ＜ 0.05) and group B 42.8％ (x2 =4.11,P ＜ 0.05).Conclusion Combined anterior and posterior operation in the treatment of cervical spondylotic myelopathy has better recovery of spinal cord function,complete decompression,significant effect,less complications,etc.,it is an effective,safe and reliable treatment.

OBJECTIVE: To investigate the use of antibiotics in the inpatients of three second-level hospitals in order to provide reference for rational use of antibiotics. METHODS: By means of retrospective study, the application of antibiotics in three second-level hospitals from Jan. to Jun. in 2008 was analyzed statistically. RESULTS: The ratio of patients using antibiotics was 67.95%. Many inappropriate phenomena still existed in application of antibiotics, such as unclear medication indications, irrational choice of drug, excessive dosage, irrational times of administration, unreasonable drug combinations, etc. CONCLUSION: It is necessary to strengthen the management of antibiotics in order to promote rational use of antibiotics.

This paper recommends the quantitative determination of endotoxin in PVC disposable transfusion set by limulus test for the first time in our country. The data obtained can act as reference material for eontroling the quality of the transfusion set. The p-nitroaniline staining combined with spectrophotometry was used in this study. The precision, sensitivty and repetition rate of this method were satisfactory. We conclude that this method is an ideal one for quantitatively determining endotoxin in PVC transfusion set.

The difference and similarity between the WEIQI theory of TCM and clinical nutrition theory of modern medicine (parenteral and enteral nutrition) was discussed. The application of two theories to clinical medicine have been extensive. The purposes are identical although they have different concepts and principles as well as methods, and two theories should be more helpful and useful if used simultaneously in practice.

By reviewing the evolution of syndrome differentiation of eight-principle, it is analyzed that a methodology of logical thinking in syndrome differentiation is the main action of eight-principle in the Science of Syndrome Differentiation. This methodology can standardize and guide other ways of syndrome differentiations. So the eight-principle is of the first position in the Science of Syndrome Differentiation and is a general program for all kinds of syndrome differentiation methodologies.

Objective To obtain excellent cultivars of Salvia miltiorrhiza cultivated in Jiangsu Province by selective breeding and provide some basic reference for systemic breeding.Methods Tanshinones(tanzhinone Ⅱ_A and cryptotanshinone) and salvianolic acids(salvianolic acid A and B) in the roots of the four cultivars of S.miltiorrhiza were determined by HPLC.The total tanshinones and salvianolic acids were determined by spectrophotometry.Biomasses of four cultivars and correlative analysis between biomasses and effective components were analyzed with Excel and SPSS software.Results Accumulating of biomasses of the four cultivars was coincident incompletely.Contents of total tanshinone,tanshinone Ⅱ_A,cryptotanshinone,salvianolic acid B,danshensu,and total salviandic acids were different.Correlative analysis showed that the relativity between biomasses and contents of effective components of four cultivars were negative correlative.Relativity among liposoluble components was distinct.Correlation coefficients between tanshinone Ⅱ_A and cryptotanshinone of four cultivars were 0.943(ZY),0.942(DY),(0.715)(XY),and 0.960(ZC),respectively.Correlation coefficients between salvialic acid A and B of four cultivars were 0.766(ZY),0.416(DY),0.841(XY),and 0.618(ZC),respectively.But the relativity between hydrosoluble and liposoluble components was not obvious.Accumulating trends of liposoluble and hydrosoluble components were different.Conclusion Contents of total diterpenoids and tanshinon Ⅱ_A in little leaf cultivars are more than the contents of other three cultivars.The qualities of other three cultivars are better than the qualities of S. miltiorrhiza recorded in Chinese Pharmacopoeia.

Objective To observe and explore the pre hospital dual antiplatelet therapy combined with emergency rescue nursing application effect in patients with acute myocardial infarction. Methods 70 cases of patients with acute myocardial infarction in our hospital and treated from April 2014 to April 2017 as the research object, randomly divided into control group and observation group, 35 cases in each group. the control group of patients first aid given aspirin 300mg, clopidogrel 600mg chewing and routine emergency care procedures for emergency patients in the observation group were given aspirin 300mg and clopidogrel 600 mg chawing simultaneously gives the optimization of emergency nursing procedure, and comparative analysis of two groups of patients. Results There were statistically significant differences of the duration of rescue, the time of emergency balloon dilatation, the time of access to the emergency room and the number of hospital stay between the observation group and control group (P<0.05); the success rate of rescue (97.14％) in the observation group was statistically significant (P <0.05) compared with the control group (80.00％, P<0.05); there was a significant difference in the recurrence rate (2.86％) between the observation group and the control group (17.14％, P<0.05); there was significant difference in nursing satisfaction (97.14％) between the observation group and the control group (77.14％, P<0.05). Conclusion The better implementation of hospital patients with acute myocardial infarction before dual antiplatelet therapy combined with emergency care program.

Objective To explore the clinical significance of a new model with emergency triage information system (ETIS) combined with pre-registration during the emergency process.Methods A total of 1 000 patients who applied the new model with ETIS combined with pre-registration from January to April 2016 were assigned as the observation group,while another 1 000 patients from January to April 2015 who applied the conventional model with triages were assigned as the control group.The used time of triage,used time of registration,collection conditions of vital signs,and accurate rate of triage were compared between these two groups.Meanwhile,mortality,rescue success rate,omission rate,excessive-detection rate,transferring surgical duration,as well as door-to-needle time (DNT) and percutaneous coronary intervention (PCI) time in red region,were compared between these two groups.Results Subjects in the observation group had notably shorter used time of triage and registration,prominently higher accurate rate of triage,markedly better collection conditions of vital signs (P ＜ 0.01),obviously lower mortality,omission rate,excessive-detection rate,transferring surgical duration,DNT and PCI time (P ＜ 0.05),and significantly higher rescue success rate (P ＜ 0.01),compared with patients in the control group.Conclusions The application of the new model with ETIS combined with pre-registration has high clinical significance.It greatly optimizes the emergency process,significantly shortens patient's triage and registration time,promotes the accurate rate of triage,allows the rapid and complete collection of vital signs,and more importantly,it also improves the time for rescuing critical patients,tremendously raises the rescue success rate and reduces mortality.

Objective:To investigate the effect on uterine involution in caesarean section.Methods:1230 cases of primipara were divided into group A(cesarean section,n=650),group B(spontaneous delivery,n=580),measuring the level of uterine fundus,descending speed of uterine fundus,observing the duration of lochia,routinely screening of uterine involution.Results:There was significant t difference between the two groups with regard to the average decending speed of uterine fundus within 5 days after delivery,group B was superior to group A;After 42 days,The cases whose lochia disappeared were 61.8%and 92.4%in group A and group B in turn;The day 5,30 and 42 ultrasound measurement of uterine volum,showing significant difference(P