Objective To explore clinical value of different administration modes applied to treat the asthma combined with chronic obstructive pulmonary disease ( COPD ) .Methods Clinical data of 180 patients with bronchial asthma combined with COPD admitted by the department of respiratory medicine of the Hospital from June 2013 to June 2015 were retrospectively analyzed, and such patients were divided into three groups including 58 patients in the control group, 60 patients in the observation group A and 62 patients in observation group B ( n =62 ) in random cross double-blind method.The patients in the control group were administered and treated with placebo ( once in the morning and at night daily respectively) , the patients in the observation group A were administered and treated with 2.5 μg of tiotropium bromide ( once in the morning and at night daily respectively), and the patients in the observation group B were treated with 2.5 μg of tiotropium bromide (once at night daily respectively), and the treatment efficacy on three groups of patients were observed.Results Average questionnaire scores of FEV1, AUC0 ~24 h , FEV1(0 ~24 h) peak valley value, PEFam , PEFpm , FEV1 and ACQ -7 in 24 hours of patients in the observation group A and observation group B, there were no statistically significant differences between two groups;Questionnaire scores of FEV1, AUC0 ~24 h , FEV1(0 ~24 h) peak valley value, PEFam , PEFpm , FEV1 and ACQ-7 in 24 hours of patients in the observation group A and observation group B were significantly higher than such indexes of patients in the control group, and the differences were statistically significant ( P <0.05 ) .Conclusion Two different dosage regimens of tiotropium bromide with daily dose of 5 μg can significantly keep the bronchus expanding for 24 h, and delivers significant efficacy on the asthma combined with COPD treatment.

This paper recommends the quantitative determination of endotoxin in PVC disposable transfusion set by limulus test for the first time in our country. The data obtained can act as reference material for eontroling the quality of the transfusion set. The p-nitroaniline staining combined with spectrophotometry was used in this study. The precision, sensitivty and repetition rate of this method were satisfactory. We conclude that this method is an ideal one for quantitatively determining endotoxin in PVC transfusion set.

Acute leukemia (AL) is an early hematopoietic stem/progenitor cell malignant cloning disease.Minimal residual disease and drug resistance are considered to be the origin of relapse and refractory.Recently,more and more evidences have shown that the interaction between leukemia cells and stromal cells or extracellular matrix in the bone marrow microenvironment can promote their survival,and enhance the resistance to conventional chemotherapy drugs.Integrin is the major molecule mediating the adhesion between leukemia cells and extracellular matrix (cellular and non cellular components),which plays a key role in cell proliferation,survival and other biological processes;however,the mechanism is not fully understood yet.This paper will review molecular mechanisms of the relationship between integrin and AL discovered recently.

The difference and similarity between the WEIQI theory of TCM and clinical nutrition theory of modern medicine (parenteral and enteral nutrition) was discussed. The application of two theories to clinical medicine have been extensive. The purposes are identical although they have different concepts and principles as well as methods, and two theories should be more helpful and useful if used simultaneously in practice.

By reviewing the evolution of syndrome differentiation of eight-principle, it is analyzed that a methodology of logical thinking in syndrome differentiation is the main action of eight-principle in the Science of Syndrome Differentiation. This methodology can standardize and guide other ways of syndrome differentiations. So the eight-principle is of the first position in the Science of Syndrome Differentiation and is a general program for all kinds of syndrome differentiation methodologies.

OBJECTIVE: To investigate the use of antibiotics in the inpatients of three second-level hospitals in order to provide reference for rational use of antibiotics. METHODS: By means of retrospective study, the application of antibiotics in three second-level hospitals from Jan. to Jun. in 2008 was analyzed statistically. RESULTS: The ratio of patients using antibiotics was 67.95%. Many inappropriate phenomena still existed in application of antibiotics, such as unclear medication indications, irrational choice of drug, excessive dosage, irrational times of administration, unreasonable drug combinations, etc. CONCLUSION: It is necessary to strengthen the management of antibiotics in order to promote rational use of antibiotics.

To define the diagnostic thought on TCM syndrome in animal model of modern medicine disease.To diagnose "apparent syndrome" outside and "hidden syndrome" inside of TCM syndrome differentiation existed possibly in the model animal by multi-aspectual indexes such as observing the appearance of modeled animal,examining the laboratory indexes and morphology,disproving by prescription and medicine etc.It is an effective method of diagnosing TCM syndrome in animal model of modern medicine disease that using multi-aspect and multi-index diagnostic standard.

OBJECTIVE:To establish an HPLC method for the determination of levels of intracellular cholesterol and cholesterol esters in foam cells derived from RAW264.7 murine macrophages so as to obtain a precise measure of foam cells and provide a technical platform for the study of the intracellular cholesterol content change.METHODS:HPLC method was applied to determine the content of cholesterol esters in the foam cells treated with OX-LDL and different concentration of pravastatin.RESULTS:OX-LDL could significantly enhance cellular intake of lipids(P

Research on the relationship between obesity and breast cancer has been deepened in recent years.Obese breast cancer patients have poorer prognosis than normal-weight patients.Weight variation is correlated with relapse in breast cancer survivors.The role of adipokines in this process can not be ignored.The correlations between adipokines,estrogen,cell proliferation,angiogenesis and breast cancer prognosis are reviewed in this article.

Objective To summarize the clinical features and risk factors of children with post-infectious bronchiolitis obliterans (PBO), who were treated with invasive mechanical ventilation because of severe pneumonia, and to provide clinical reference for early diagnosis, early treatment and improvement of prognosis of pediatric patients with PBO. Methods The pediatric patients treated with invasive mechanical ventilation because of severe pneumonia, who were hospitalized in PICU of Tianjin Children's Hospital from October 2010 to December 2015 were included in this study. The pediatric patients who were diagnosed with PBO were used as the observation group. The general characteristics, clinical presentation, treatment time, treatment results, distribution of pathogens and relevant laboratory examination results were analyzed by using SAS 9.3 statistical software. Results A total of 73 pediatric patients were included in this study, including 49 male and 24 female, and their mean age was 8 months (P25-P75:4-12), the minimum age was 1 month and the maximum age was 36 months. They were divided into PBO group (n=30) and control group (n=43). It was found that wheezing (27 cases, 90.0%), persistent cough (22 cases, 73.3%) and exercise intolerance (22 cases, 73.3%) were the main clinical symptoms in PBO group. Results of physical examination showed wheeze (24 cases, 80.0%) and crackles (21 cases, 70.0%). Mosaic perfusion (25 cases, 83.3%) was the most prominent sign in lung CT scan. More than 2/3 of the pediatric patients were found moderate and severe pulmonary dysfunction. Pathogen detection rate was 50%in PBO group, and virus was prevalent (respiratory syncytial virus 4 cases, influenza virus 1 case, measles virus 2 cases and adenovirus 4 cases). The results of Logistic multivariate regression analysis showed that the risk factors included using corticosteroids at the beginning of disease (OR=8.515, 95%CI:1.948-37.224), prolonged mechanical ventilation (OR=1.116, 95%CI:1.031-1.327) and postponing clinic time (OR=1.114, 95%CI:1.010-1.227). Conclusion Once the severe pneumonia children, with the risk factors of using corticosteroids at the beginning of disease, prolonged invasive mechanical ventilation and postponing clinic time, are found the wheezing, persistent cough and exercise intolerance, and persisting wheeze and/or crackles, the PBO relevant examination should be executed as soon as possible to diagnosis and treatment.