This paper recommends the quantitative determination of endotoxin in PVC disposable transfusion set by limulus test for the first time in our country. The data obtained can act as reference material for eontroling the quality of the transfusion set. The p-nitroaniline staining combined with spectrophotometry was used in this study. The precision, sensitivty and repetition rate of this method were satisfactory. We conclude that this method is an ideal one for quantitatively determining endotoxin in PVC transfusion set.

Research on the relationship between obesity and breast cancer has been deepened in recent years.Obese breast cancer patients have poorer prognosis than normal-weight patients.Weight variation is correlated with relapse in breast cancer survivors.The role of adipokines in this process can not be ignored.The correlations between adipokines,estrogen,cell proliferation,angiogenesis and breast cancer prognosis are reviewed in this article.

Objective To explore the clinical efficacy of combined anterior and posterior operation in the treatment of cervical spondylotic myelopathy.Methods 50 patients with cervical spondylotic myelopathy,in accordance with anterior,posterior surgery and combined anterior and posterior operation three different surgical methods,were divided into the treatment group A(21 cases),group B(16 cases) and group C(13 cases).The surgical efficacy was analyzed.Results Before treatment,the JOA score among the three groups had no significant difference [Cgroup (9.3 ± 2.3) points,B group (9.4 ± 1.4) points,A group (9.2 ± 2.2) points].After treatment,the JOA score in group C (14.5 ± 3.4) points was significantly higher than that of group A (12.6 ± 1.2) points (t =3.09,P ＜0.05) and group B (11.3 ± 1.4) points (t =4.62,P ＜ 0.05).The excellent and good rate of C group was 76.9％,which was significantly higher than that in group A 43.8％ (x2 =3.84,P ＜ 0.05) and group B 42.8％ (x2 =4.11,P ＜ 0.05).Conclusion Combined anterior and posterior operation in the treatment of cervical spondylotic myelopathy has better recovery of spinal cord function,complete decompression,significant effect,less complications,etc.,it is an effective,safe and reliable treatment.

The difference and similarity between the WEIQI theory of TCM and clinical nutrition theory of modern medicine (parenteral and enteral nutrition) was discussed. The application of two theories to clinical medicine have been extensive. The purposes are identical although they have different concepts and principles as well as methods, and two theories should be more helpful and useful if used simultaneously in practice.

By reviewing the evolution of syndrome differentiation of eight-principle, it is analyzed that a methodology of logical thinking in syndrome differentiation is the main action of eight-principle in the Science of Syndrome Differentiation. This methodology can standardize and guide other ways of syndrome differentiations. So the eight-principle is of the first position in the Science of Syndrome Differentiation and is a general program for all kinds of syndrome differentiation methodologies.

OBJECTIVE: To investigate the use of antibiotics in the inpatients of three second-level hospitals in order to provide reference for rational use of antibiotics. METHODS: By means of retrospective study, the application of antibiotics in three second-level hospitals from Jan. to Jun. in 2008 was analyzed statistically. RESULTS: The ratio of patients using antibiotics was 67.95%. Many inappropriate phenomena still existed in application of antibiotics, such as unclear medication indications, irrational choice of drug, excessive dosage, irrational times of administration, unreasonable drug combinations, etc. CONCLUSION: It is necessary to strengthen the management of antibiotics in order to promote rational use of antibiotics.

OBJECTIVE:To establish an HPLC method for the determination of levels of intracellular cholesterol and cholesterol esters in foam cells derived from RAW264.7 murine macrophages so as to obtain a precise measure of foam cells and provide a technical platform for the study of the intracellular cholesterol content change.METHODS:HPLC method was applied to determine the content of cholesterol esters in the foam cells treated with OX-LDL and different concentration of pravastatin.RESULTS:OX-LDL could significantly enhance cellular intake of lipids(P

To define the diagnostic thought on TCM syndrome in animal model of modern medicine disease.To diagnose "apparent syndrome" outside and "hidden syndrome" inside of TCM syndrome differentiation existed possibly in the model animal by multi-aspectual indexes such as observing the appearance of modeled animal,examining the laboratory indexes and morphology,disproving by prescription and medicine etc.It is an effective method of diagnosing TCM syndrome in animal model of modern medicine disease that using multi-aspect and multi-index diagnostic standard.

Objective:To investigate the effect on uterine involution in caesarean section.Methods:1230 cases of primipara were divided into group A(cesarean section,n=650),group B(spontaneous delivery,n=580),measuring the level of uterine fundus,descending speed of uterine fundus,observing the duration of lochia,routinely screening of uterine involution.Results:There was significant t difference between the two groups with regard to the average decending speed of uterine fundus within 5 days after delivery,group B was superior to group A;After 42 days,The cases whose lochia disappeared were 61.8%and 92.4%in group A and group B in turn;The day 5,30 and 42 ultrasound measurement of uterine volum,showing significant difference(P

Objective:To inhibit GR expression in macrophage RAW264.7 cells of mouse by RNAi and Cre-loxP system.Methods:Two pairs of RNAi target sequence against GR gene were designed and inserted into pBS/U6-Neo vehicle.Recombinant plasmid constructed by Cre-loxP system and RNAi was identified by PCR and sequencing.The recombinant plasmid and Cre-ERT2 plasmid was transfected into the mouse macrophage RAW264.7 cells,in which GR expression was measured on mRNA and protein level respectively by RT-PCR and Western blot.Results:GR expression on mRNA and protein level was significantly inhibited by the two recombinant plasmid against GR gene.Conclusion:Combination using of RNAi and Cre-loxP system could inhabit GR expression in mouse macrophage RAW264.7 cells.