OBJECTIVE To investigate Epstein-Barr virus (EBV) infection and clinical symptoms among hospitalized children by detection of EBV in their throat swabs. METHODS During from Oct 2004 to Apr 2006, EBV-DNA was detected from throat swabs of inpatients in pediatric ward by the technology of fluorescence quantitative polymerase chain reaction. RESULTS Totally 3429 cases were detected, 738 cases were positive. The positive rate was 21.5%. In different age groups, the rate of EBV infection was 18.8% for ≤3 years old, 26.5% for 4-7 years old, 29.0% for 8-11 years old, and 31.7% for 12-15 years old, respectively. The rate of EBV infection increased significantly with age increasing (P

Objective To investigate the protection of renal function and the prevention of acute renal failure (ARF) in patients with malignant obstructive jaundice in perioperative period of radical resection. Methods A series of clinical interventions had been taken since 2004 in our treatment team, including control of endotoxemia, depression of biliary tract before operation, maintenance of adequate effective blood volume, nutritive support, administration of mannitol and low dose of furosemide, and avoidance of disseminated intravascular coagulation. The incidence of perioperative ARF in 206 patients with malignant obstructive jaundice who had been radically resected from 2000 to 2007 was retrospectively studied, and the RIFLE criteria was used for ARF classification. This study was progressed in two periods. The first one was from Jan. 2000 to Dec. 2003, and the second one was from Jan. 2004 to Dec. 2007. Results After 2003, the proportion of radical resection rose from 44.8% to 57.1% (P

Objective To explore the status of college students’ attachment and locus of control, and explore their relationship. Methods A total of 198 college students were surveyed adopting Experiences in Close Relationships Inventory and Internality, Powerful Others and Chance Questionnaire. Results The distribution of college student′s attachment type was not balanced,the proportion of dismissing type,security type, fearful type, preoccupied type accounting for 43. 9%,31. 3%,21. 7%,3. 1% respectively. The scores of internality, chance and powerful others were (27. 59 ± 5. 44),(17. 92 ± 6. 66),(17. 70 ± 7. 32) respectively. Attachment avoidance correlated negatively with internality(r= -0. 259,P<0. 01), and positively with chance and powerful others(r=0. 206,0. 221,P<0. 01);besides there was a positive correlation between attachment anxiety and chance,powerful others(r=0. 378, 0. 410,P<0. 01). Attachment avoidance had prediction on internality(R2 =0. 067,P<0. 01). Attachment anxiety had prediction on chance and powerful others(R2 =0. 143,0. 168,P<0. 01). Conclusion College students’ at-tachment has significant effects on locus of control.

OBJECTIVE:To reduce the risk of high-risk drugs dispensing in PIVAS. METHODS:Main measures of risk con-trol of high-risk drugs in PIVAS of our hospital were introduced,i.e. establishing high-risk drug risks control group and high-risk drugs list,pharmaceutical staff undertaking all job of PIVAS except finished product distribution,information management of high-risk drugs application procedure,clinical pharmacist's participation,etc. The effects of error and ADE evaluation and manage-ment in PIVAS were analyzed statistically to summarize the experiences. RESULTS & CONCLUSIONS:During 2012-2014,the in-cidence of high-risk drugs related error was in low level in PIVAS of our hospital(0.009‰,0.004‰,0.002‰)and decreased year by year. The number of error decreased from 8 in 2012 to 3 in 2013 and 2 in 2014. It is important for risk control to enhance drug check,new staff training and PIVAS staff professionalization in pharmaceutical,and extablishing warning mark of high-risk drugs.

AIM: To investigate the regulatory effect of the adenosine triphosphate binding cassette transporter A1 (ABCA1) on apolipoprotein E secretion from human THP1 macrophages.METHODS: Differentiation of THP1 macrophages from monocytes was stimulated with phorbol 12-myristate 13-acetate. The macrophages then were incubated with factors which regulate ABCA1 expression. After periods of incubation, apo E secreted in the medium and synthesized in the cell was determined with ELISA, and apo E mRNA espression was detected with Northern blot.RESULTS: An increase in apo E secretion from THP1 macrophages was observed by 8 h of incubation with 8-Br-cAMP, an activator of ABCA1 expression (P

Objective To investigate the prevalence of Helicobacter pylori infection and the incidence of gastric mucosal lesions in type 2 diabetes mellitus(DM)and diabetic kidney disease(DKD)patients with dyspepsia symptoms.Methods A total of 241 type 2 diabetic patients and 69 non -diabetic subjects with dyspeptic symptoms were enrolled in the study.Gastroduodenal lesions were observed by gastrointestinal endoscopy and the presence of Helicobacter pylori infection was identified by rapid urease test and serum IgG antibodies to Helicobacter pylori.Urine albumin excretion rate(UAE)at 24 hours was measured in all subjects.According to the urinary albumin excretion rate,patients were classified into diabetes mellitus group(DMgroup,with UAE <30mg/24h),diabetic kidney disease group 1(DKD group 1,with UAE 30mg/24h to <300mg/24h)and diabetic kidney disease group 2(DKD group 2 >300mg/24h).Estimated glomerular filtration rate were above 60mL·min -1 ·(1.73m2 )-1 in the three groups of patients.The 69 cases of non -diabetic subjects were used as the control group.Results The prevalence of H pylori infection in the DKD group[145 /72(62.5%)]and the DKD group 2[34 /53(64.15%)]were significantly higher than those in the control group[28 /65(43.1%)](χ2 =3.901,P =0.04;χ2 =4.223,P =0.03)and the DM group [27 /63 (42.9%)](χ2 =4.104,P =0.04;χ2 =5.116,P =0.03).No significant differences of H.pylori prevalence were detected between the DKD groups(χ2 =1.304,P =0.29)as well as the DMgroup and the control group (χ2 =0.723,P =0.40).Gastroscopy results showed that the incidence of normal endoscopic performance in the DMgroup was higher than that of the control group(57.1% vs.38.5%,χ2 =4.612,P =0.03).There were no significant differences between the control group and DM group,DKD1 group and DKD2 group in the incidence of gastric mucosal lesions (Superficial gastritis:χ2 =1.206,0.912,0.707;erosive gastritis:χ2 =1.422,1.836 0.870;duodenal ulcer:χ2 =243.1, 1.716,2.233;gastric ulcer:χ2 =1.440,0.971,1.322 and esophagitis:χ2 =2.116,2.318,2.488,all P >0.05). Conclusion Diabetic nephropathy patients are more susceptible to Helicobacter pylori infection.There is no significant difference in the infection rate of Helicobacter pylori between type 2 diabetic nephropathy with microalbuminuria and with macroalbuminuria.There are no significant differences in the type of gastric mucosal lesion between Type 2 diabetes mellitus,diabetic nephropathy patients and non diabetic patients.

Objective: To explore value of 6-minute walking in rehabilitation therapy of patients with chronic heart failure (CHF). Methods: A total of 78 CHF patients were selected from department of geriatrics of our hospital. They were randomly and equally divided into 6min walking test (6MWT) group (received 6-min walking training based on routine treatment, twice/d) and routine treatment group. After six weeks, 6min walking distance (6MWD), heart rate and left ventricular ejection fraction (LVEF) were compared between two groups before and after treatment. Results: After six weeks, there were significant improvements in related indexes in both groups, P<0.05 all; compared with routine treatment group, there were significant increase in 6MWD [(307.6±39.3) m vs. (503.4±44.4) m] and LVEF [(45.3±17.9) % vs. (58.7±19.2) %], and significant decrease in heart rate of recovery period after 6MWT [(73.3±2.9) times/min vs. (65.7±2.1) times/min] in 6MWT group, P<0.05 all. Conclusion: Six-minute walking can significantly improve symptoms of heart failure and enhance exercise tolerance, and it possesses important value for recovery of heart function in these patients.

Objective To study the effects of Danlong Xingnao Tablet on neuronal apoptosis and expression of TNF-? after focal cerebral ischemia reperfusion in rat hippocampus. Methods The model of middle cerebral arteries occlusion (MCAO) were performed with intraluminal filament blockade. By using in situ terminal deoxynucleotidyl transferase-mediated dUTP-biotor nick end labeling (TUNEL) and immunohistochemistry staining, the number of apoptotic cells and TNF-? positive cells were detected in the sham-operated group, the ischemia reperfusion group, Danlong Xingnao Tablet group and nimodipine group after the middle cerebral arteries of SD rats occluded for 1 h and reperfused for 24 h.Results Compared with the sham operation group, the number of apoptotic cells and TNF-? positive cells of the model group increased significantly (P

Objective To investigate the protective effects of daidzein (DD) on myocardial hypertrophy and fibrosis induced by pressure overload in rats and to study its mechanism. MethodsMyocardial hypertrophy and fibrosis model of rats induced by pressure overload was prepared by constricting abdominal aorta. The operated rats were randomly divided into sham operated control group, aorta-constricted model group, and three DD groups (30, 60, and 120 mg/kg). Four weeks later, the heart-weight (HW), left ventricular weight (LVW), the ratio of HW/BW and LVW/BW (LVI), and the cardio-myocyte diameters (MD) after dying by HE color were measured. The content of collagen and nitric oxide (NO), the activity of calcineurin (CaN) and Na+, K+-ATPase, Ca2+-ATPase in the left ventricle were quantified with spectrophotometry. The angiotension Ⅱ (AngⅡ) in the left ventricle was messured with radioimmunoassay. Results In aorta-constricted model group, the ratio of HW/BW, LVI, and MD as well as the content of collagen and AngⅡ, the activity of CaN in the left ventricle was significantly increased, and Na+, K+-ATPase, Ca2+-ATPase activity and NO content in the left ventricle were obviously decreased. After treatment of the left ventricular with DD, NO content, Na+, K+-ATPase, Ca2+-ATPase activity were significantly increased, the content of collagen and of AngⅡ and the activity of CaN in the left ventricle and the ratio of HW/BW, LVI, and MD were significantly reduced. ConclusionDD has protective effects on ventricular remodeling in rats with myocardial hypertrophy and fibrosis induced by pressure overload and its mechanism may be related to raising NO content and reducing the level of AngⅡ and the activity of CaN.