Objective To analyze the changes of myeloid-derived suppressor cells (MDSCs) in peripheral blood and serum nitric oxide in tumor-bearing mice, and to explore their correlation and functions in tumor progression. Methods Orthotopic transplantation tumor model of hepatocarcinoma in mice was established. At the 7th, 14th, 21st day after tumor implantation, the proportion of peripheral blood MDSCs was tested by flow cytometry, and serum nitric oxide was detected using the method of nitric acid reductase. Results The proportion of peripheral blood MDSCs at the 7th, 14th, 21st day after tumor implantation in tumor-bearing mice was significantly higher than that in normal mice [(8.65±1.01)%, (14.20±2.52)%, (27.51± 2.98) % vs. (2.51 ±0.44) %, P<0.05]. The level of nitric oxide at the 7th, 14th, 21st day after tumor implantation in tumor-bearing mice was significantly lower than that in normal mice [(72.02 ±5.05) μmol/L, (94.70 ±3.34) μmol/L, (78.90 ± 8.19) μmol/L vs. (57.03 ±16.94) μmol/L, P<0.05]. The level of serum nitric oxide was positively associated with the proportion of MDSCs (r=0.689, P<0.01). Conclusion The proportion of peripheral blood MDSCs and the level of serum nitric oxide in mice are increased with the tumor progression, and there is a positive correlation between them.

Objective To discuss the effect of music therapy on anxiety control of patients with maxillofacial cancer.Methods 140 cases in head and neck surgery department were divided into the test group and the control group with 70 cases in each group from March 2004 to November 2005.The test group received music intervention based on routine nursing.The degree of anxiety was evaluated on the first day of hospitalization(T1),the previous day before the operation(T2),the first day after operation(T3)and the third day after operation after listening to music.The control group only received routine nursing.The condition of anxiety at different time points was evaluated and compared.Results The scores of anxiety(SAS)in the two groups were statistically different(P＜0.05).The intra-group comparison of anxiety degree in the test group(T1-T2,T1-T3,T1-T4)had statistical difference(P＜0.05).The intra-group comparison of anxiety degree in the control group(T1-T2,T1-T3)had no statistical difference(P＞0.05).While evident difference existed in T1-T4 in the control group.P＜0.05.Conclusion Music intervention facilitated the rehabilitation of anxiety condition in cancer patients.

Objective It has been shown that aprotinin and tranexamic acid are equally effective in protecting platelet and reducing postoperative bleeding. The aim of this study was to compare the effects of aprotinin and tranexamic acid on blood coagulation. Methods Thirty patients undergoing elective valve replacement were randomly divided into two groups : (1) aprotinin group (AP, P

Objective To study the influence factors on the enflurane uptake in child with congenital heart disease(CHD).Methods Sixty children aging less than 5 years with CHD were randomly divided into four groups: in group A 15 children with atrial septal defect(ASD)or ventricular septal defect (VSD) inhaled enflurane before and after CPB;in groups B 15 children with ASD or VSD did only after CPB,in group C 15 children with tetralogy of fallot (F4) did before and after CPB;in group D 15 children with F4 did only after CPB.The concentration of enflurane (F I) was kept at 0.5%.Following inhalation of enflurane,the end tidal alveolar concentration (F E) was measured in 30 min and the time of uptake equilibrium (Tue,F E/F I=1) was recordcd.Results In group C,Tue before CPB was shorter than that before CPB and after CPB in group A,that in group B and group D (P

OBJECTIVE:To evaluate the economics of penehyclidine hydrochloride and atropine in the treatment of acute organophosphorus poisoning (AOPP).METHODS:The information of 118 AOPP patients were collected and divided into group A (59 cases) and B (59 cases) according to therapy plan.There were 22 cases of mild poisoning,20 cases of moderate poisoning and 17 cases of severe poisoning in group A.There were 21 mild cases,21 moderate cases and 17 severe cases in group B.Based on routine treatment,group A was given Penehyclidine hydrochloride injection intramuscularly with initial dose of 1 mg (mild),2 mg (moderate) and 4 mg (severe).Group B was given Atropine sulfate injection intravenously,with initial dose of 2 mg (mild),5 mg (moderate) and 10 mg (severe).Both received maintenance treatment according to patients condition and stopped taking medicine after symptoms disappeared.Clinical efficacies,the time of acetylcholinesterase recovery and ADR were observed in 2 groups.The economics of therapy plans for mild,moderate and severe poisoning were evaluated in 2 groups by cost-effectiveness analysis.RESULTS:There was no statistical significance in total response rate of mild poisoning or the time of acetylcholinesterase recovery between 2 groups (P＞0.05).Total effective rates of moderate and severe poisoning in group A were significantly higher than group B,and the time of acetylcholinesterase recovery was significantly shorter than group B,with statistical significance (P＜0.05).There was no statistical significance in the incidence of ADR in mild,moderate and severe poisoning patients (P＞0.05).Cost-effectiveness ratio of penehyclidine hydrochloride was similar to that of atropine in mild poisoning patients;that of penehyclidine hydrochloride were significantly lower than that of atropine in moderate and severe patients.It was inline with the results of sensitivity analysis.CONCLUSIONS:Based on routine treatment,penehyclidine hydrochloride is similar to atropine in therapeutic efficacy of AOPP and the time of acetylcholinesterase recovery.For moderate and severe AOPP patients,penehyclidine hydrochloride is significantly better than atropine in improving therapeutic efficacy and the time of acetylcholinesterase recovery.The safety of 2 drugs are satisfactory;penehyclidine hydrochloride possesses cost-effectiveness advantage.

Objective:To assess the clinical and microbiologic effects of ultrasonic scaling with simultanous irrigation with chlorhexidine(CHX), conventional ultrasonic scaling or subgingival irrigation. Methods: 20 teeth in 7 patients with periodontitis were equally divided into test and control group. Patients in the test group were treated by irrigation with CHX during ultrasonic scaling(Master400),those in the control with irrigating after scaling(MiNi ). Clinical assessments of bleeding on probing (BOP),probing depth (PD) were made just before and 7 and 14 days after treatment. The subgingival microbiologic examination was performed before and 0,7 and 14 days after treatment. Result: Significant reductions in PD and BOP were observed in both groups 7 and 14 days after treatment(P

Objective To explore the function of MDA on diabetic nephropathy .Methods Glomerular mesangial cells ( GMC) were pretreated with MDA at a final concentrations of 0μmol/L, 1μmol/L, 5μmol/L, 10μmol/L and 50 μmol/L.MTT assay was used to examine the viability of GMC ) .AnnexinV-FITC was used to evaluate effect of MDA on cell apoptosis .RT-PCR and western blot were used to analyze the expression of Nrf 2, HO-1 andγGCL.Results MDA treatment inhibited GMC viability in a dose-dependent manner .MDA at the concentration of more than 5 μmol/L induced mass production of ROS in GMC ( P<0.05 ) .In addition , antioxygen of tBHQ may relieve MDA-induced reduction of cell viability .MDA inhibited the expression of HO-1 , γGCLand Nrf2 ( P <0.05 ) .Conclusions MDA inhibites GMC viability and promotes the cell apoptosis by ROS production through in-hibiting Nrf2/HO-1-γGCL.

Objective To investigate the efficacy and safety of autohemotherapy therapy in ASST positive chronic idiopathic ur‐ticaria .Methods One hundred and twenty cases of chronic idiopathic urticaria treated in our department from April ,2012 to Janu‐ary ,2013 were divided into treatment group and control group ,60 cases in each group .Patients in treatment group were given oral ioratadine (10 mg/d) ,supplemented by autohemotherapy ;patients in control group were only given oral ioratadine (10 mg/d) .Both of the two groups were treated with twelve weeks ,then observed the effectiveness and safety of two kinds of treatment .Results The effective rate of treatment group and control group were 68 .33% and 48 .33% respectively ,and there was significant difference between the two groups (P<0 .01) .Conclusion Autohemotherapy combined antihistamine was of high efficiency and safety in the treatment of ASST positive chronic idiopathic urticaria ,while the long‐term curative effect remains to be observed .

Objective:The changes and distribution of myeloid-derived suppressor cells (MDSCs) in spleen of tumor-bearing mice was studied,and its function in tumor escape was investigated.Methods:Orthotopic transplantation tumor model of hepatocarcinoma in mice was established.At the 7th,14th,21st day after tumor implantation,the proportion of MDSCs in spleen was tested by flow cytometry.The localization of MDSCs and semi-quantitative analysis was conducted by immunohistochemistry.Results:The proportion of spleen MDSCs in tumor-bearing mice was significantly higher than in normal mice (P

Objective To investigate the effects of 5-fluorouracil (5-FU) on the distribution, pro-portion and immunosuppressive activities of myeloid-derived suppressor cells (MDSCs) in a mouse model of liver cancer.Methods Orthotopic transplantation of H22 cell line was performed to establish the mouse model of orthotopic liver cancer.Forty mice were randomly divided into four groups (n=10) on the 7th day after transplantation.Three different doses of 5-FU (10 mg/kg, 30 mg/kg and 50mg /kg) and 0.4 ml of PBS were respectively given to the mice in each group for 10 consecutive days .A control group without canc-er cell transplantation was set up correspondingly .The distribution of MDSCs in mice liver tissues was detec-ted by immunohistochemistry .The proportions of MDSCs in spleen tissues were tested by flow cytometry . The levels of IFN-γand Arginase-I ( Arg-I) in serum samples were analyzed by enzyme-linked immunosor-bent assay.The activities of nitric oxide synthetase (NOS) in serum samples were tested by chemical colori-metry.The proliferation of T lymphocytes was assessed by 3-(4,5-Dimethylthiazol-2-yl)-2, 5-diphenyltet-razolium bromide (MTT) test.Results 5-FU reduced the tumor size and alleviated the adhesion between liver tissues and abdominal cavity in mice with liver cancer in a dose-dependent manner .The proportions of MDSCs in liver and spleen tissues from mice in four model groups were significantly higher than those in con - trol group (P<0.05).The highest proportions of MDSCs were observed in PBS treated group , followed by 10 mg/kg of 5-FU treated group , 30 mg/kg of 5-FU treated group and 50 mg/kg of 5-FU treated group .Ex-cept for comparing between PBS treated group and 10 mg/kg of 5-FU treated group, there were significant differences with the proportions of MDSCs in liver tissues among other groups (P<0.05).The levels of Arg-I and the activities of NOS in mice with liver cancer were higher than those in control group (P<0.05).The mice in PBS treated group showed the highest level of Arg-I and the strongest activities of NOS that were de-creased as concentrations of 5-FU increased( P<0.05).The levels of IFN-γin serum samples from mice with liver cancer were lower than those in control group , and the lowest level was detected in PBS treated group.IFN-γlevels were increased as concentrations of 5-FU elevated.Except for comparing between PBS treated group and 10 mg/kg of 5-FU treated group , significant differences with the levels of IFN-γin serum samples (P<0.05) were observed among other groups .No significant differences with the proliferation of T cells were observed among all groups after 24 hours of culture (P>0.05),but that were decreased in model groups than those in control group after 48 hours of culture (P<0.05).T cells from mice in PBS treated group showed the lowest level of proliferation .T cell proliferation was enhanced upon the treatment of 5-FU in a dose-dependent manner .Except for comparing between PBS treated group and 10 mg/kg of 5-FU treated group, significant differences with T cell proliferation were observed among other groups (P<0.05).The proportions of MDSCs in liver and spleen tissues were positively correlated with the levels of Arg -I and the activities of NOS, but negatively correlated with IFN-γlevel and T cell proliferation at the time point of 48 h.Conclusion To a certain extent , 5-FU could reduce the numbers of MDSCs in liver and spleen tis-sues from mice with liver cancer , inhibit immunosuppressive activities of MDSCs and restrain the growth of the tumor in a dose-dependent manner .