Objective To observe the effects of misoprostol on prevention of postpartum hemorrhage.Methods 200 cases of full-term pregnancy intravaginal labor were randomly divided into misoprostol groups (n=100) and control groups (n=100).The misoprostol groups were treated with misoprostol 400 μg PO after labor and control groups were treated with oxytocin 20 U IV after labor.Results The average time interval of the third stage was (6.10±3.35) min in misoprostol groups,whereas (10.00±3.45) min in controls.There was significant difference between two groups (P＜0.01).The average quantity of blood loss in 2 h after labor was (150.30±34.00) ml in misoprostol groups,whereas (240.60±57.30) ml in controls.Conclusion Better effects of misoprostol than oxytocin were proved on preventing postpartum hemorrhage.

Objective To improve recognition of the processus articularis arthropathy.Methods CT findings of the processus articularis arthropathy was analysed.Results According to the CT findings of the 94 cases processus articularis arthropathy,CT signs and the syndrome of this disease were summarized five types:①zygapophyseal hypertrophy and osteophyte formation;②stricture or asymmetry of articular space ;③vacuum phenomenon of articular space ;④pachynsis or calcification of articular capsule ;⑤articular instability.Conclusion The processus articularis arthropathy is a very high incidence of a disease.The CT scanning is very important significance to the disease.

Objective To discuss the diagnostic value of the lumbar intervertebral disc degeneration by CT scanning.Methods The clinical representation and CT view of 809 cases with degenerative lumbar intervertebral disc were retrospectively analyzed.Results According to the different CT representation character,the degenerative lumbar intervertebral disc may be divided into the lumbar intervertebral disc denaturation bulge in 96 cases and the lumbar intervertebral disc herniation in 713 cases.Conclusion CT scanning is a value method to diagnosing degenerative lumbar intervertebral disc.

Objective To improve the knowledge of lumbar posterior marginal cartilagionous node.Methods CT findings of lumbar posterior marginal cartilaginous node in 52 cases were analyzed.Results Three CT characters were:summarized cartilagionous node,centrum posterior marginal osseous absence and protruding follicle ring fragment.Conclusion CT scanning hes defiuite diagnositic value for the lumbar posterior marginal cartilaginous node.

BACKGROUND:Adipose-derived stem cel s are totipotent stem cel s in the adipose tissue, and have the function of self-renewal and multi-directional differentiation. Human adipose-derived stem cel s are ideal seed cel s with stable genetic milieu and few rejections.
OBJECTIVE:To extract human adipose-derived stem cel s from human omental adipose tissue and to identify the cel s by adipogenic and osteogenic induction.
METHODS:Omental adipose tissues were col ected from surgical patients to isolate and culture adipose-derived stem cel s using type I col agenase digestion, filtration and centrifugation. Cel growth was observed and proliferative curve of human adipose-derived stem cel s were drawn by cel counting method to calculate the doubling time at logarithmic growth phase. After adipogenic and osteogenic induction, induced cel s were identified using oil red O and alizarin red staining, respectively.
RESULTS AND CONCLUSION:Human adipose-derived stem cel s were successful y isolated from the omentum tissues of surgical patients. Adherent cel s were fusiform-shaped and like fibroblasts. The growth curve of passage 3 cel s was in S shape, and the doubling time was 45.90 hours. After adipogenic and osteogenic induction for 2 and 3 hours, respectively, oil red O staining showed unequal-sized orange fat droplets, and alizarin red staining showed typical calcified nodules that were in orange. These findings indicate that adipose-derived stem cel s have the adipogenic and osteogenic capacity.

AIM: To optimize the extraction and purification of water-soluble active ingredient of Ferula sinkiangensis.METHODS: First,the comprehensive scoring system was set up to reflect the change of the extraction rate and ferulic acid coutent.in combination with the uniform design.RESULTS: The optimized extracting process was as follows: adding 8 times water and extracting twice,every time lasting 2.5 h.The optimized centrifugation purificating process consisted of: concentration ratio (1 ∶ 3),35 minutes centrifugal time,4 000 r/min rotation speed.The optimized ZTC1 + l-Ⅱ clarificant purification process was as follows: concentration ratio was 1 ∶ 22,the content of pacificator was 22% ,purification time was 1 h.CONCLUSION: The optimized extraction is available,reasonable,and reproducible.The results of pharmacodynamic test show that centrifugation is suitable to the purification of extract liquid.

Objective To study the value of common X-ray and CT scanning in the spinal bursting fracture (abridged English:BF).Methods By the frontal,lateral X-ray film and CT scanning,the imaging manifestations of BF in 43 cases were analysed,contrasted,summarised and classified .Results Among the BF 43 cases,the classifications were type A(10 cases),type B(15 cases),type C(2 cases),type D(9 cases) and type E(7 cases).The three-post injure had 34 cases,the two-post injure had 9 cases,the spinal canal narrowing:0?was 8 cases,1?was 12 cases,2? was 23 cases;the rear spinal edges discontinuation was 35 cases.Conclusion The diagnostic value of X-ray film to BF is reflecting the bones injured change of spinal fracture type,flexion and damaged rear edge.The CT scanning advantage is showing the fracture lines trend,relative scope,degree of spinal canal deformation and narrow,infering the pressed situation and injured spinal cord.The two methods combine and confirm each other in order to help clinical diagnosis the right treating.

0.05).The grade of antibiotics tended to decline with the increase in of dressing change freqnency so it could decrease the expenditure of antibiotics.The rate of fungal infection lowered while hospitalization did not appear to be prolonged.CONCLUSIONS After the analysis of the use of antibacterials for burn patients,we find that the proper use of antibiotics and the increase in dressing change would lower the grade of antibiotics and expenditure,at the same time they do not increase the rate of bacterial infection and fungal infection,and do not prolong the duration of patients hospitalization time.

ObjectiveTo analyze the risk factors of pulmonary infection for critically ill ICU patients with tracheotomy,and investigate the methods to prevent nosocomial lung infection. Methods94 patients in ICU with pulmonary infection after tracheotomy from March 2008 to March 2010 were analyzed retrospectively,they were set as the observation group.100 patients in synchronization without pulmonary infection after tracheotomy were set as the control group.The general condition,diagnosis and treatment methods and care,the environment,etc.were studied,the risk factors for nosocomial pulmonary infections after tracheotomy were analyzed.The care methods and points to prevent lung infections for critically ill ICU patients after tracheotomy were summarized. ResultsThe study showed that the respiratory system diseases in critically ill patients,smoking history,low seniority of nursing staff,long-term use of large dose of antibiotics,application of H2 blockers and antacids,and many patients in the same ward were risk factors for critically ill patients with pulmonary infection. ConclusionsLung infection after tracheotomy in critically ill ICU patients was affected by many factors.In the actual operation,we should targeted to avoid infection led by treatment and care factors,environmental factors,control and prevention measures should especially be strengthened for patients with respiratory diseases to prevent lung infection.

Objective To evaluate the proliferation and invasion potential of human colon cancer SW480 cells transfected by AP-2α gene in vitro. Methods pcDNA3.1 (+)-AP-2α was created by cloning AP-2α eDNA into the EcoRI site of poDNA3.1 (+). LipofectamineTM 2000 Reagent was used to mediate the transfection of pcDNA3.1(+)-AP-2α and pcDNA3.1 (+), and normal SW480 celia were cultured as a negative control. The mRNA and protein level of AP-2α in the cells of each group were detected 48 h after being transfected with plasmids above by RT-PCR and Western blotting analysis. The cell proliferation and invasion potential were examined by colony formation assay and Transwell invasion assay respectively. Results Lack of AP-2α protein expression in SW480 cells was verified by western blot analysis. After being transfected with AP-2α gene, the mRNA amount and protein expression increased dramatically, while the colony formation efficiency decreased(P <0.05), the cell proliferation in soft agar was inhibited, and the ability of its invasion dropped off(P <0.05) in vitro. Conclusion AP-2α gene suppresses the proliferation and invasion potential of human colon cancer SW480 cells in vitro.