Nutritional therapy is an important part of therapy of chronic diarrhea in children. The nutritional status is very important to growth and development of the children patients. The supplementation of appropriate amount of protein,abundant energy,vitamin,minerals and probiotics can improve the nutrition and promote growth and develop-ment,then remission the development of disease and decrease the incidence of the complication.

Objective The changes of Glutamic acid(Glu) and GABA immunoreaction in the cerebral cortex and hippocampus of rats with seizure induced by IL-1? and IL-6 were studied.To explore the mechanism of IL-1? and IL-6 in epilepsy. Methods Experimental rats were randomly divided into 3 groups:control group,IL-1? group,and IL-6 group.After intracerebroventricular injection of relevant reagents for 120?min,behaviour changes were observed,Glu and GABA were examined by means of immunohistochemistry in the cerebral cortex and hippocampus of rats. Results The behaviour observation indicated that no seizure happened in control group,seizure in middle degree was observed in IL-1? group and IL-6 group.Compared with in control group,the immunoreaction of Glu showed that the expression was significantly increased in IL-1? group and IL-6 bgroup,while GABA was obviously decreased after intracerebroventricular injection IL-1? or IL-6 at 120?min.Conclusion The machanism of that IL-1? or IL-6 particpated in promotion and abduction epilepsy may be through increasing Glu and decreasing GABA in the cerebral cortex and hippocampus.The nerve excitation is enhanced and then epilepsy occurred.

Objective To study the genetic toxicity of aluminium trichloride on reproductive cells of male mice.Methods Twenty healthy male KM mice were randomly divided into four groups,the experimental groups(50,75,100 mg/kg AlCl3 respectively) and the normal control group(0.9% NS),all of the groups were exposed by intraperitoneal injection,2 consecutive days with one day interval,for 2 weeks.The variation of body weight and index of testicle was observed,olive tail moment was evaluated by comet assay while sperm nucleus immaturity rate was examined by fluorescent staining sperm nucleus.Results Compared with the negative control group,the index of testicle in each AlCl3 treated group decreased significantly(P

Objective To analyze reasons for the generation of unqualified blood specimens in coagulation test in tumor patients and to develop countermeasures ,so as to ensure the quality of samples prior to analysis .Methods Blood specimens received from outpatients in Tumor Hospital of Yunnan Province in 2012(40 253 specimens) ,2013(46 756 specimens) and first quarter of 2014 (14 566 specimens)were retrospectively analysed .Unqualified rate was used to describe situation of unqualified specimens ,and the distribution and changes of unqualified specimens were compared among the three years .Results The unqualified rate of blood specimens in coagulation test in 2012 and 2013 was 0 .57% and 0 .96% ,respectively .Reasons for unqualified blood specimens in 2012 was ,in order ,specimens agglutination ,insufficient amount of specimen ,excessive amount of specimen ,wrong container ,no specimen .Reasons for unqualified blood specimens in 2013 was ,in order ,specimens agglutination ,insufficient amount of specimen , excessive amount of specimen ,bar code error ,contaminated specimen ,wrong container ,no specimen ,hematocrit≥55% ,repeated in‐spection .Conclusion Coagulation test requires high quality specimen and quality assurance prior to analysis is particularly impor‐tant .The clinical laboratories should strengthen the links between the nursing and clinical departments ,timely communicate and feedback situation of unqualified specimens ,find the cause together and develop and implement effective improvement measures ,in order to ensure the quality of specimens on the steps before analysis .

Objective To investigate clinical efficacy and safety of carboprost tromethamine injection combined with low B -Lynch suture for intractable postpartum hemorrhage placenta previa.Methods 125 cases of intractable postpartum hemorrhage placenta previa were divided into two groups according to treatment(n =62)with the observation group(n =63),the control group was administered oxytocin,and the line in the placental separation surface 8 interrupted suture;the observation group was used carboprost tromethamine injection combined with low B -Lynch suture.Clinical efficacy,blood loss,bleeding,postoperative bleeding 2h,24h after blood loss,transfusion rate and index sex hormone levels were compared before and after treatment.Results (1)After treatment,total effec-tive rate of the control group and observation group was 77.42%(48 /62)and 95.24%(60 /63)respectively.There was a statistically significant difference between the two groups(P <0.05);(2)Blood loss,bleeding time,2h after blood loss,blood loss and transfusion rate after 24h in the observation group were (1 608.37 ±43.01)mL,(8.89 ± 1.09)min,(101.18 ±8.73)mL,(221.17 ±20.58)mL and 4.76%,respectively,which were significantly lower than those of the control group [(1 992.28 ±55.62)mL,(12.83 ±1.77)min,(145.49 ±12.19)mL,(255.54 ± 23.30)mL and 16.13%](t =4.559,5.128,3.928,3.382,χ2 =5.559,all P <0.05);(3)before and after treatment indicator hormone(E2 ,FSH and LH)levels were not significantly different(P >0.05),and after treatment,the index level differences were not statistically significant(P >0.05);(4)the two groups during treatment,liver and kidney functions were normal,three cases of the control group and two cases of the observation group occured nausea and vomiting.Conclusion Carboprost tromethamine injection combined with low B -Lynch suture has significant effect, a small amount of bleeding,high security for intractable postpartum hemorrhage placenta previa,which should be promoted.

Objective To measure the serum levels of endostatin in lung cancer patients in different stages and with histological types, and explore the clinical value of serum level in early diagnosis of lung cancer.Methods ELISA was used to detect the level of serum endostatin in 81 patients with lung cancer (lung cancer group), 23 patients with benign lung disease (benign lung disease group), 20 healthy controls (healthy control group). The serum levels of endostatin were analyzed in lung cancer patients in different stages and with histological types.Results The serum level of endostatin in lung cancer group was (2.53±8.75) ng/mL, significantly higher than that in benign lung disease group (4.63±1.12) ng/mL and healthy control group (4.53±1.24) ng/mL. In lung cancer group, the serum level of endostatin of stage Ⅰsubgroup and Ⅱsubgroup was significantly higher than that of stage Ⅲ subgroup. The differences were statistically significant. There were statistical differences of serum level of endostatin among the lung cancer patients with different histological types.Conclusion Serum endostatin might be used as an indicator of early diagnosis of lung cancer.

Objective To discuss the effect of music therapy on anxiety control of patients with maxillofacial cancer.Methods 140 cases in head and neck surgery department were divided into the test group and the control group with 70 cases in each group from March 2004 to November 2005.The test group received music intervention based on routine nursing.The degree of anxiety was evaluated on the first day of hospitalization(T1),the previous day before the operation(T2),the first day after operation(T3)and the third day after operation after listening to music.The control group only received routine nursing.The condition of anxiety at different time points was evaluated and compared.Results The scores of anxiety(SAS)in the two groups were statistically different(P＜0.05).The intra-group comparison of anxiety degree in the test group(T1-T2,T1-T3,T1-T4)had statistical difference(P＜0.05).The intra-group comparison of anxiety degree in the control group(T1-T2,T1-T3)had no statistical difference(P＞0.05).While evident difference existed in T1-T4 in the control group.P＜0.05.Conclusion Music intervention facilitated the rehabilitation of anxiety condition in cancer patients.

Objective To identify the spectrum of pathogenic microorganisms of urinary tract infection and the drug resistance in a hospital setting. Methods The pathogenic microorganisms isolated from 3117 mid-stream urine samples of patients admitted in Hangzhou First People's Hospital from 2005 to 2007 and their drug resistance results were retrospectively analyzed. Results Bacteria were the most prevalent microorganisms in the urinary tract infection, and followed by fungus, mycoplasma and chlamydia. Escherichia eoli accounted for the largest proportion of gram-negative bacteria, in which the ESBLs positive strains accounted for 51.2%, and their drug resistance rate was much higher than that of ESBLs negative strains. Main gram-positive coccobacteria was all sensitive to vancomycin, and relatively sensitive to nitrofurantnin and ampicillin. Conclusions Escherichia coli continue to prevail upon the spectrum of pathogenic microorganism of the urinary tract infection, and the fungus, mycoplasma and chlamydia infections are rising. Antibacterial agents should be used under the guidance of drug sensitivity test, and the combined use should be avoidd.

Recently, single Chinese medicine extract on the treatment of diabetic retinopathy became popular. Some progress of experimental and clinical studies of single Chinese medicine extract on the treatment of diabetic retinopathy has been made. Thus, this paper summarized the recent progress of both clinical and experiment studies on Single Chinese medicine extract for diabetic retinopathy.

Objective To investigate the effect of competitive N-methyl-D-aspartate (NMDA) receptor antagonist LY274614 [(?)-6-phosphonomethyl-decahydroisoquinolin-3-carboxylic acid] on the development of morphine tolerance and dependence.Methods Male Spraque-Dawley rats were rendered tolerant and dependent by subcutaneous injection of morphine(15mg/kg body weight) three times a day for 10 consecutive days. LY274614 (2, 4, 6 mg/kg body weight) was also given subcutaneously by subcutaneous injection. Antinociception was measured by tail-flick (TF) test. Tail was exposed to the heat source(a beam of high intensity light). The time from the beginning of exposure to removal of tail from the path of the heat source was taken as latency. The baseline TF latency without medication was 4-5 seconds. A ten-second maximum exposure to the heat source was used to minimize damage to tissue during the multiple measurements. Morphine prolonged TF latency. With the development of tolerance TF latency gradually returned to baseline value. Physical dependence on morphine was assessed by abstinence syndrome precipitated by subcutaneous injecting naloxone 10 mg/kg on the tenth day. According to method of Blasig, the number of jumping/30min after naloxone injection was recorded as an index pf abstinence syndrome. Rats were randomly divided into 8 groups (n=6-8). Each group received morphine 15 mg/kg or normal saline (NS) 1.5 ml/kg+LY274614 (2.0,4.0,6.0 mg/kg) or NS, group 1: morphine+NS; group 2: NS+NS; group 3:morphine+LY274614 (2mg/kg); group 4: NS+LY274614 (2mg/kg); group 5: morphine+LY274614 (4mg/kg); group 6: NS+LY274614 (4mg/kg); group 7: morphine+LY274614 (6mg/kg); group 4: NS+LY274614 (6mg/kg).Results LY274614 itself did not have analgesic action, but if used with morphine, it did inhibit the development of tolerance. In group 5 and 7 the decrease in TF latency was more gradual than that in morphine+saline group. 4 and 6 mg/kg LY274614 reduced the number of jumping/30min following naloxone injection.Conclusions LY274614 can inhibit morphine tolerance and dependence rendered by consecutive subcutaneous morphine injection.