Objective:To investigate infection status of hepatitis B virus in ChongQing children. Method:We detected the HBV serology markers(HBVM) hy ELISA in 8060 children in Children's Hospital of Chnngqing Medical University. Results:HBsAg positive rate was 4.44%,anti—HBs positive rate 46.56%. 3795 cases were negative in HBVM(47.08%);259 cases were positive in HBsAg,HBeAg and anti-HBc(3.21%);80 cases were positive in HBsAg, anti-HBe, anti-HBc (0.99%),and Pre-S1 positive rate was 3.51%. Conclusion:Among the 8060 children who came to our hospital,the infection rate of HBV is 4.44%,lower than in the adults. Pre-S1 is related with HBeAg,and it can reflect the status of HBV multiplication and infectivity to some extent.

OBJECTIVE:To evaluate the inhibitory effect of hydrocortisone on fentanyl-induced cough reflex(FIC).METHODS:160 patients undergoing scheduled operation on abdominal region were randomized to receive Normal Saline 100 mL(Group Ⅰ)or hydrocortisone 100 mg added to 100 mL Normal Saline iv gtt(Group Ⅱ);15 minutes later,both groups were injected intravenously with fentanyl(2 ?g?kg-1)with injection time of 2 seconds.The frequency and the onset time of cough as well as the adverse drug reactions in the two groups were observed.RESULTS:The FIC incidence in Group Ⅱ was significantly lower than in Group Ⅰ(10% vs.35%),showing significant differences between the two groups(P

Objective:establishment a Laboratory Information Management System.Methods:The interface is designed by visual basic 6;the database managed by ACCESS;the enquiry and statistic work is finished by SQL sentences;data is received by utilizing Active X control;and the report work is finished by Printer object and DataReport designer.Results:This system has over-all functions,beautiful interface and resistant database,it performs the common statistics and supervisory functions basically.Conclusion:There are some innovative things such as three-steps management pattern,dynamic contrast current record with historical information,variegated seek and statistics,humanization design and utility tools.on the whole,it is a software which can service the needs of clinical work and has a broad future.

Objective To evaluate the prevention and treatment of prosthetic infections after tension free hernioplasty. Methods The clinical date of 12 prosthetic infections admitted from May 2005 to May 2008 were analyzed retrospectively. Patch was inserted under prefascial retromuscular site in 8 cases, between fascial edges in 2, Mesh plug and patch Lichentstein in one each. As for the material used there were flat knitted polypropylene in 7 cases, polytef in 1 case and composite prosthesis in 4 cases. Treatments were based on prothetic materials and levels infected. Infectious patchs were removed completely in 8 cases and partially removed in 2 cases, Open dress change with patchs left in situ in 2 cases. 3 cases in 10 cases of removed patchs were given immediate suturation with closed drainage. Results Infection was cured in all cases without mortality. There was no hemorrhage and injury of bladder during the procedures. No retention of urine, pain and other complications during postoperation. Change of dressing was made from 7 days to 6 months( median 2 weeks). Selected antibiotic was used from 3 to 7 days (average 4.5 days). All patients were followed up from 18 to 78 months (average 38.5 months) without hernia recurrence and infection. Conclusion It was most important to prevent infections after tension-free hernioplasty. Management should be individualized according to different type of patch used and the surgical procedure in hernia repair.

Objective To analyze the clinical characteristics of adult intussusception and improve its diagnostic and treatment levels.Methods Data of 80 cases of adult intussusception treated in the First Affiliated Hospital of China Mdeical Unuversity between January 2001 and December 2015 were reviewed retrospectively.Results 95％ patients had abdominal pain,34％ had bloody stool and 24％ had abdominal mass,8％ presented a triad of abdominal pain,bloody stool and abdominal mass.As confirmed by laparotomy 48％ were enteric intussusception,40％ were ileocolic intussusception,and 12％ colocolonic intussusception.The diagnosis rate of abdominal uhrasonography was 60％;CT established the diagnosis in 97％ cases.74％ underwent malignant or benign tumor resection,10％ underwent intestinal resection for inflammatory bowel disease.10％ were cured by simple reduction.4 cases received intussusception reduction and appendectomy.Conclusions Tumors were causes of adult intussusception in most case.CT is most accurate in diagnosis.Laparoscopy can be used for difficult diagnosis.

Objective To investigate the functional changes of endothelial cells (EC) in non-thrombosis vein segment (ECNT) in rats with experimental venous thrombosis (VT). MethodRats were divided into normal, control, and thrombosis group caused by legating a portion of iliac vein with 30 animals in each group. Endothelium was sampled from superhepatic IVC. Samples were studied by electroscopy, immunohistochemistry, and Western blotting for apoptosis. ResultsMorphological and functional changes of the ECs were observed in sampled superhepatic IVC. Compared with normal and control group the apoptosis rates(P

Objective To analyze postoperative complications of surgical therapy for deep vein thrombosis (DVT) of the lower extremity. Method From January 2001 to January 2008 vena cava filters were placed in 171 DVT cases before surgery. Fogarty catheter (73 cases), Amplatz thrombectomy device (ATD) (55 cases), Acolysis ultrasound ablation(43 cases) were used to extract the thrombi in iliac and proximal femoral vein. The full extraction of thrombi in distal end was facilitated by compressing and massaging the legs in all cases. Iliac venous stenosis or occlusion was managed by interventional therapy, and temporary femoral arteriovenous fistula were carried out routinly. Result Operations were successful in 157 cases(70 cases in Fogarty group, 52 in ATD group,35 in Acolysis ultrasound ablation group), and failed in 14 cases (3 cases in Fogarty group, 3 in ATD group, 8 in Acolysis ultrasound ablation group). Permanent,retrievable and temporary vena cava filters were placed in 51,32 and 88 cases respectively. Stenosis or occulsions of the iliac vein was found in 143 cases and stents were placed after balloon dilatation in 41 cases. During the operation, residual thrombi was found in 80 cases. Vessel perforation and rupture were complicated in 14 cases, thrombosis adhering in eava vein filters in 18 cases. There was no mortality and no pulmonary thromboembolism. During the follow-up, we found iliae vein restenosis in 21 cases, thrombosis recurrence in 36 cases, stents displacement in 6 cases, and stents fracture in 2 cases. Conclusion Surgical therapy is effective for DVT of the lower extremities.

Objective　To evaluate the results of radical resection for the treatment of Budd-Chiari syndrome (BCS) caused by short segmental lesions in inferior vena cava (IVC), and major hepatic veins (MHV).　Methods　In this series, resection of IVC occlusive segment and orthotopic artificial vascular graft transplantation were performed in 42 patients. Resection of major hepatic vein, hepatic venous plasty and orthotopic transplantation with artificial vascular graft were performed in 10 patients. Resection of major hepatic vein, anastomosis between hepatic vein and right artrium in 3 cases. Percutaneous transhepatic angioplasty in 10 cases.　Results　One patient died of complication. 58 cases were followed up for an average of 30 months. 3 cases in interventional group suffered recurrence and one in surgical group had recurrence.　Conclusions　The treatment of BCS by resecting inflicted segment of IVC or hepatic veins and orthotopic artificial vascular graft transplantation effect a radical cure in most cases.

Objective To investigate the clinical efficacy of endovascular treatment for iliac vein compression syndrome (IVCS) in 112 cases. Methods From September 2011 to June 2015, 112 cases of IVCS (Cockett syndrome) were treated in our hospital with endovascular methods .The case numbers of different CEAP ( clinical etiological anatomical pathophysical ) classifications were C2 (45 cases), C3 (23 cases), C4 (19 cases), C5 (11 cases) and C6 (14 cases).All the cases were diagnosed by venography .The operations started from puncturing the ipsilateral veins .The stenotic degree and compressed intensity of iliac vein were evaluated by the minimum diameter with low pressure balloon .And then a stent was placed into after percutaneous transluminal angioplasty ( PTA) when residual stenosis was more than 50%.The occlusive segment was dilated by 8 mm diameter balloon firstly , and catheter directed thrombolysis treatment was applied if thrombus was detected .The PTA and stenting were performed when the thrombus was completely removed .The improvement of clinical symptoms was observed after operation and the patients were followed up by ultrasound and venograghy . Results Nine cases were given PTA only , and the other 103 cases underwent stent placement . Postoperative venography showed collateral vessels were disappeared in 22 cases, obviously decreased in 56 cases and mildly decreased in 34 cases.The average difference of swelling leg ’s circle was diminished from (2.56 ±0.88) cm pre-operation to (0.93 ±0.71) cm post-operation, while the average area of skin ulcer was diminished from (6.34 ±3.78) cm2 pre-operation to (2.13 ±1.88) cm2 post-operation, all of which were statistically significant (t =20.24 and 7.19,P=0.000).A total of 86 cases were successfully followed up during a period from 2 months to 45 months (average, 19.2 months).The patency rate of treated iliac vein 12 months after operation was 76.2%in the PTA group, and in the PTA with stenting group was 96.7%, 88.6%, and 86.1%, respectively, at 12, 24, and 36 months after operation, in which a statistical significance was detected (log-rank χ2 =30.32, P=0.000).The patency rate in diameter ≥16 mm group was 95.2%and 95.2%, respectively, at 12 and 24 months after operation, while in diameter <16 mm group was 97.0%and 93.3%, respectively, in which no statistical significance was detected (log-rankχ2 =0.39, P=0.532).Thrombotic complication was found in 2 cases during the follow-up period , including 1 case of iliac vein thrombosis at 1 month and 1 case of thrombus in contralateral leg 8 months later .According to the results of venography in 78 cases at 12 months after operation , restenosis was found in 5 out of 6 cases in the PTA group, and the patency rate in the stent placement group was 95.8%(69/72).No stent migration was found . Conclusion Comfortable clinical results were obtained after endovascular therapy for iliac vein compression syndrome , and the application of stenting could increase the middle and long term patency rate of treated vessels .

Objective To evaluate catheter-directed thromlysis (CDT) through three different approaches in combination with intervention for acute deep venous thrombosis (DVT) of the lower extremities. Methods In this study 137 acute DVT cases were enrolled. CDT was performed through small saphenous veins in 107 cases,through the great saphenous veins in 21 and through popliteal veins in 9.Iliac vein balloon dilation was performed in 66 cases,and stents were placed in 60 cases. Results The limb edema reduction rates between small saphenous vein group and great saphenous vein group and popliteal vein group were of no significant difference (82.3％ ±7.6％ vs 81.6％ ±6.0％ vs 83.9％ ±6.1％,P＞0.05).The difference of rates of thrombolysis ( 63.5 ％ ± 7.7％ vs 66.9％ ± 8.4％ vs 66.1％ ± 2.7％ )between the 3 groups was not statistically significant (P ＞ 0.05 ). The mean time required for the cannulation was shorter in great saphenous vein group than the other two groups [ (7.3 ± 0.3 ) min vs (20.8 ± 1.1 ) min and (15.7 ±0.6) min,P ＜ 0.05 ].There were 12 cases complicating incision bleeding during thrombolysis in small saphenous vein group,in this group there were 5 cases complicating phlebitis and 8 complicating saphenous nerve injury.The complication rates in great saphenous vein group was lower (P ＜ 0.05).Follow-up made in 112 cases for 3 -25 months.There were 97 cases without limb edema.While test by venography or color Doppler,89 cases showed venous patency and rethrombosis developed in 8 cases. Conclusions Catheter-directed thrombolysis with iliac venous intervention was an effective method for the treatment of acute deep venous thrombosis.CDT through great saphenous vein is easier with less complications.