Objective To investigate the isolation,incubation and identification of murine yolk sac mesenchymal stem cells(YS-MSCs). Methods Murine yolk sacs were harvested on the 10th day of postcoitus microisolation.Yolk sac cells were obtained after the yolk sacs were digested by type Ⅰ collagenase of 0.1% for 1 hour.Adherent cells were cultured in DMEM containing 10% FBS and 1% SP,and passaged when they became subconfluent.The ultrastructure of YS-MSCs was observed under transmission electron microscope;the surface markers of YS-MSCs were detected by flow cytometry;the alkaline phosphatase(AKP) expression of YS-MSCs was tested;and cytochemical changes of YS-MSCs were observed with cytochemical detection.Adipogenic differentiation of YS-MSCs was induced by 1?mol/L dexamethasone and 10mg/L insulin,and oil red O was used for fat staining. Results YS-MSCs were obtained in vitro,and most of them were of spindle shape;under transmission electron microscope,there were microvilli on the surface of YS-MSCs,there were abundant mitochondria,rough endoplasmic reticulum and golgi complex in the endochylema.The cell nuclei of YS-MSCs were big and the shape of cell nucleus was irregular;the analysis of flow cytometry suggested that the primary and first generation of YS-MSCs were positive for CD44,CD105,and the expression of CD34 was small;Cytochemistry showed that YS-MSCs were positive for glycogen,and negative for SB and AKP;Adipogenic differentiation of YS-MSCs was induced by 1?mol/L dexamethasone and 10mg/L insulin;accumulation of lipidrich vacuoles appeared in the cells,positive for oil red O staining.Conclusion The biological characteristics of murine yolk sac mesenchymal stem cells are similar to,and more primitive than,those of adult mesenchymal stem cells.It suggests that YS-MSCs may be used as an ideal resource of seed cells of tissue engineer.

Objective To explore the clinical analysis of 65 cases of elderly patients with gastric carcinoma. Methods The clinical data of 65 cases of elderly patients with gastric carcinoma from October 2002 to September 2007 were analyzed retrospectively. Results 34 eases took place a variety of complications in this group of 26 cases of patients,and they are 5 cases of incision dehiscence,2 cases of anastomotie leakage, 1 cases of duodenal stump leakage,1 cases of gastric dysfunction,ll cases of heart failure,14 cases of lung infection,3 cases of urinary tractin-fection, two case of intra-abdominal infection, two cases of intestinal obstruction, 1 case of postoperative died. Conclu-sion As early as mid-term elderly gastric cancer should be perfrmed radical resection,at the same time,prevent and treat complications actively.

Objective To study the hypoglycemic effect of polysaccharide from stigma maydis and to explore its therapeutic mechanism preliminarily.Method The diabetes mice models were induced by tetraoxypyrimidine.The concentration of blood sugar was determined by glucose oxidase test.The influence of the stigma maydis polysaccharide on glycometabolism and its repair effect on damaged glycometabolism organs of diabetic mice were observed respectively by content determination of glycogen and by the measurement of activities of relative enzymes(superoxyde dismutase and glutathione peroxidase).Results The stigma maydis polysaccharide could decrease the blood sugar,promote the synthesis of glycogen and repair the damaged glycometabolism organs of diabetic mice.Conclusion The stigma maydis polysaccharide exhibits a good therapeutic effect on diabetic mice,and has a good development prospect.

Objective To investigate the effect of thymosin α1 on immun ologic function and cytokines in the abdominal sepsis patients.and naddiscuss the therapeutic function of thymoin α1 in sepsis.Methods Thirty-eight sepsis patients admitted between May 2006 and May 2007 in the Hospital of Weifang Medical University.Patients were divided into the treatment group(20 cases)and control group(18 cases)randomly.Thymosin α1 was used in the treatment group on the basis of infection controlled.The concentration of cytokines(TNF-α,IL-6,IL-10)and the percent age of CD3,CD4,NK cell were observed.Results The concentrations of TNF-α and IL-6 were increased significantly in bothgoups.But it was more prominently in the control group.The concentration of IL-10 and the percent age of CD3,CD4,NK cell increased more significantly in thymosinod treatment group than those in the control group.Conclusions Thymosincd canmodulate the release of TNF-α,IL-6,IL-10 and mitigate the inflammatory reaction,which may improve the immunologic function.

Objective To establish the biological reference interval of procalcitonin(PCT) applicable for children in Chongqing.Methods Serum PCT level were detected in 120 healthy children with age from 0 to 16 years, including 73 cases of male and 47cases of female children, by using by Maglumi2000 plus PCT analysis system.All data was evaluated according to EP28-A3c document to establish the biological reference interval.Results Data of PCT levels were with non-normal distribution, and without statistical difference between children of different ages and genders(P>0.05).The biological reference interval of PCT was less than or equal to 0.038 μg/L.Conclusion It might be important to establish a usefully biological reference interval in different laboratories with relative detection system, especially for children.

In order to synthetically train students to do scientific researches independently,inspire their enthusiasm and go-aheadism about study,and improve the quality of experimental teaching,we have been exploring to update experimental content and reform experimental system for many years,and have commenced a number of "Series Experiments".The setup of"Series Experiments" which means several separate experiments are organized together by their internal relations has already showed us a favorable effect.

AIM: To investigate the effect of Shexiang Baoxin Pill(SXBXP) on endothelial in stable angina pectoris patients and its underlying mechanisms. METHODS: Sixty-eight stable angina pectoris patients were randomly divided into two groups,the conventional group(34cases),SXBXP treatment group(34cases).The conventional group was treated with standard treatment,and SXBXP group was treated with standard treatment and SXBXP.FMD,NO,ET,TXB_2,6-Keto-PGF-1a were determined before and after three months treatment. RESULTS: FMD,NO,6-Keto-PGF-1a of SXBXP group were (9.35%?0.78%)、(77.25?6.36)?mmol/L、(93.87?(10.28))?/(ng/L) after treatment,ET、TXB_2 were(81.15?5.43) pg/mL、(43.02?4.19)?/(ng/L).There were significant improvement as compared with in SXBXP group before treatment and in conventional group after treatment(P

Objective To investigate the clinical utility of keyhole craniotomy for Cholesteatmas in the cerebello-pontine angle region presenting as Trigeminal neuralgia by using keyhole craniotomy. Methods Clinical data of 35 pa-tients with Cholesteatmas in the cerebellopontine angle region presenting as Trigeminal neuralgia was analyzed retrospec-tively. All patients received suboccipital retrosigmoid keyhole craniotomy. Thirty cases who received conventional craniot-omy group were served as control group. A comparative analysis was conducted to assess the difference between these two groups in microsurgical methods, effects and complications. Results All patients were confirmed with MR diagnosis. In keyhole group, all patients had no pain symptoms of trigeminal neuralgia after surgical operation. The symptoms disap-peared immediately in 33 cases and gradually disappeared in 2 cases within one month following keyhole craniotomy. The tumors were totally removed in 25 cases and almost completely in 10 cases. The surgical duration was shorten in key-hole group than in conventional group (126±48 vs. 216±66 min;t=2.536, P<0.05). The amount of bleeding was smaller in keyhole group than in conventional group (91.3±52.2 vs. 186.3±65.4 mL;t=2.163, P<0.05). Postoperative neurologi-cal function was assessed in House-Brackmann. In keyhole group, there were 32 cases in class Ⅰ and 3 in grade Ⅱ. Two patients had hearing loss which was improved gradually in three months. In conventional group, there were 25 cases in class Ⅰ and 5 in grade Ⅱ (χ2=4.158, P<0.05). Postoperative hearing evaluation (AAO-HNS) revealed that there were 33 cases in grade A , and 2 in grade B in the keyhole group, whereas there were 27 cases in grade A and 3 in grade B in the conventional group(χ2=5.167, P<0.05). There were no relapse of tumors and death during 3 month to 5 years follow-up. Conclusions The suboccipital retrosigmoid keyhole craniotomy is a valid choice for Cholesteatmas in the cerebellopontine angle region presenting as Trigeminal neuralgia.

ObjectiveTo discuss the diagnostic value of MRI in female patients with stress urinary incontinence (SUI).Methods All SUI patients underwent pelvic MRI examination.The 16 patients mean age was 58 years (range,48 -66 years),disease course was 6 years (range,2 -15 years).All patients underwent transobturator tension-free tape (TOT) procedures.The 28 volunteers with no pelvic diseases were set as control,Mean age was 45 years (range,30 -55 years).Results28 Volunteers' urethral support ligaments appeared as low signal intensity broad linear structures in both T1 WI and T2WI images.Display rate:periurethral ligament was 89％ (25 cases) ; paraurethral ligament was 75％ (21 cases),unilateral 25％ (7 cases) ; pubourethral ligament,in each distal 100％ (near,28 cases),10％(medium,3 cases),7％ (distal,2 cases); suburethral ligament was 100％(28 cases). Levator ani muscle in T1WI,T2WI showed Moderate- intensity signal and the display rate was 100％.16 SUI patients showed varyious degrees of laxity and rupture of urethral supporting ligaments which occurred in one group (4 cases) or multi-group (12 cases) ligaments.The ligament laxity showed that periurethral ligament 14 cases,paraurethral ligament 2 cases and proximal pubourethral ligament 10 cases.The ligament rupture was seen in periurethral ligament 2 cases,paraurethral ligament 5 cases,pubourethral ligament 6 cases and suburethral ligament 7 cases.16 patients showed a relaxation of the levator ani muscle changes,including 4 cases with cervical and vaginal wall prolapsed.Conclusions MRI could clearly show the weak of support structures around the urethra in female SUI patients,and showed the cervical and vaginal prolapsed.Therefore,MRI may provide imaging evidence for clinical diagnosis and treatment.