The purpose of this investigation is to evaluate the excitability of the sympathetic nerves in burned rats. Pre ganglionic discharges from the cervical and the epinephronic sympathetic nerves and post-ganglionic discharges from the renal sympathetic nerve fibers were recorded in rats anaesthized with urethane. After burning, the discharges of the sympathetic nerves of the rats at first showed continuous high frequency soon followed by a decline. After that, the sympathetic efferent electrical activity became very active again, displaying a high amplitude and high frequency.These results suggest that the sympathetic nerves system exhibits periodic excitatory changes in burned rats with a excitation-inhibition-excitation cycle.

An animal model of acute lung injury was reproduced by intravenous injection of E. coli endotoxin to burned rats. The histological changes of lung were studied using transmission electron microscope. It was found that leukocytes accumulated in the pulmonary capillaries and adhered to endothelia. The WBC count decreased gradually. The level of MDA, lung coefficient and lung water content were increased. The SOD activity was decreased. Pretreatment with SOD could improve the changes mentioned above. This study suggests that oxygen radicals play an important role in acute lung injury induced by burns complicated by endotoxemia.

The functional changes of pulmonary surfactant (PS) in rats of acute lung injury induced by scald companied with endotoxemia were studied The surface properties of the bronch-alveolar lavags fluid (BALF) and the total phospholipids extracted frome it were assessed on a modified Whilhemy film balance. The results showed hat the total phospholipids, phosphatidylcholine, and phosphatidyl glycerol in the BALF were decreased the total proteins in the BALF were increased and the function of PS of the BALF were reduced which may be caused by increased serum proteins in the alveoli and decreased surface activity of phospholipids themselves.

Objective To describe and explore the practice of eye care in ICU,in order to make a specified training plan and guideline on eye care,and to improve the skill in eye care of ICU nurses. Methods A survey was conducted in 92 ICU nurses from 2 general hospitals in Huizhou city with non-random sampling using a self- made questionnaire to investigate their eye care of patients. Results The nursing practice of eye care in ICU was urged to be improved,there was no standard on eye care. Conclusions The knowledge of ICU nurses needs to be enhanced,and guideline on eye care is urged to be worked out,so that practice of eye care would be improved.

Objective To detect the proteus and analyze the drug-resistance in intensive care unit.Methods From Jan 2005 to Dec 2006,proteus was detected by Disc diffusion test(K-B method)to study the antimicrobial resistance in ICU and general ward,and compared the drug-resistance.Results The isolating rate of proteus was 80.9% in sputum,and proteus mirabilis accouted for 80.4%.The resistance rates to ciprofloxacin,amikacin,cefotaxim,ceftazidime,cefmetazole and piperacillin/Tazobactam were between 64%~77%,and 32%~41% in imipenem and cefoperazone/sulbactam.Compared with general ward,the antibiotic resistant rate existed significant difference.And the antibiotic resistant rate of other type proteus had significant difference between ICU and general ward.Conclusion Properly detecting Proteus in time is imporrant to use antibiotic and to avoid nosocomial outbreak infections by Proteus.

Objective To explore changes of gray matter volume in patients with obsessivecompulsive disorder (OCD) in Chinese Han population using optimized voxel-based morphometry (VBM) ,and investigate its relationship with clinical symptoms. Methods Twenty patients with OCD and 20 age,sex and handedness matched healthy controls were scanned using 3D-T1 images on a 3.0 T MR system. The high resolution T1WI was preprocessed according to the optimized VBM protocol in Statistical Parametric Mapping (SPM5). Two-sample t test was performed to characterize the differences of the gray matter volume (GMV) between the OCD patients and healthy controls, and the correlations between the GMV and symptom severity and cumulative illness duration were examined using Pearson correlation in SPSS 16. 0, respectively.Results Compared to controls, OCD patients demonstrated increased GMV in left thalamus, right thalamus and left cerebellum after false discovery rate (FDR) correction. No areas of significantly decreased GMV was observed in OCD patients in relative to healthy controls. The mean eigenvalue ranged from 0. 5782 to 0. 889 representing the left thalamus volume of OCD patients was 0. 6813 ± 0. 0718, and that ranged from 0. 5546 to 0. 9062 was 0. 6869 ± 0. 0808 tor right thalamus. The mean eigenvalues were positively correlated in bilateral thalamus (r = 0. 94, P ＜ 0. 01). Conclusion Using optimized VBM, the current research indicates that the pathophysiology of OCD is associated with GMV abnormalities not only in corticostriato-thalamo-cortical (CSTC) circuit, but also in the cerebellum.

Isolated perfused rat hearts were subjected to regional ischemia followed by reperfusion and then tested for responsiveness to acetylcholine (Ach) and nitroglycerin (NTG). The effects of L-Arginine (L-Arg) and NG-monomethyl-L-Arg (L-NMMA) on the endothelium-dependent (ED) and independent (EI) vasodilator were also observed. It was found that both 15 and 30min ischemia followed reperfusion resulted in a decrease in ED vasodilation of Ach. ED vasodilation gradually renewed after 90min reperfusion in the 15min ischemic group, not in the 30min ischemic group. No significant decrease in EI vasodilation of NTG in all groups. L-Arg accelerated the renewing of ED vasodilation of Ach in the 15 min ischemia-reperfusion group, not in the 30min ischemia-reperfusion group. L-NMMA was an inverse action. The results indicate that brief ischemia followed by reperfusion can induce coronary endothelium dysfunction which may be related to the decrease of endothelium derived relaxing factor.

Objective To investigate the effect of intensive control of residual amount in stomach on prevention of ventilator associated pneumonia(VAP)during the process of enteral nutrition. Methods 96 patients with mechanical ventilation(MV) more than 48 hours were randomly divided into the case group and the control group, they were treated with intensive control and common control of residual amount in stomach respectively. The incidence rate of VAP, mortality rate, time of ventilation and hospitalization time were compared between the two groups. Results The incidence rate of VAP was 20.83% in the case group and mortality rate was 18.75%,the time of MV was(15.21±2.36)days, the hospitalization time was(22.38±12.34)days. The incidence rate of VAP wag 39.58%.mortality rate was 39.58%,the time of MV wag(19.85±4.58)days, the hospitalization time was(29.46±15.24)days in the control group. Conclusions Intensive control of residual amount in stomach could be helpful to decrease the incidence of VAP and mortality rate of patients with mechanical ventilation using nasal feeding, also shorten the time of MV and the hospitalization time.

Objective: To investigate the changes of myo cardial contractile function during myocardial stunning in calcium overload rats and the protective effects of tetrandrine. Methods: Forty-six rats were randomized into control, myocardial ischemia, myocardial stunning, low and high dose of tetrandrine groups. Another 10 rats were used to identify the calcium overload. vitamin D3 (0.3 million Unit/kg) and nicotinic acid were adm inistered. After 16 d when calcium overload occured, left anterior descending ar tery was ligated. Twenty minutes of myocardial ischemia followed by 60 min of re perfusion was induced. The contractile function parameters were determined dynam ically. At the end of experiment, myocardial cytosolic ［Ca2+］i was deter mined in various groups. In tetrandrine groups, tetrandrine (62.2 or 93.6 μmol/ kg ) was administered by gastrogavage daily.After 16 d, the rats undergone the e xperiments mentioned above. Results: Sixteen days after vitamin D3 , nicotinic acid were given, ［Ca2+］i increased by 2.6 folds (146.8±10.8 ) vs (368.5±22.6) nmol/L, (P<0.01). Whereas, ［Ca2+］i in tetrand rine groups were (210.8±16.4) and (198.6±15.3) nmol/L, which were significantl y lower than that of calcium overload group. Twenty minutes of myocardial ische mia resulted in the decrease of dp/dtmax and Vmax in all groups with the most si gnificant in stunning and calcium overload groups. The contractile function rest ored gradually after reperfusion. At all time points, dp/dtmax and Vmax in both tetrandrine groups were higher than those in both stunning and calcium overload groups. And effect with higher dose of tetrandrine were more significant than in low dose of tetrandrine. After 60 min of reperfusion, dp/dtmax in stunning, cal cium overload, low and high dose of tetrandrine groups were 49.7%, 51.5%, 71.0% and 83.4% of that in control, respectively， and Vmax were 55.0%, 49.8%, 73.9% and 77.5% of that in control, respectively. Conclusion: T he myocardial contractile function in vitamin D3-induced calcium overload gro up is impaired. On basis of myocardiocyte calcium overload, transient ischemia l eads to myocardial stunning. At the stage of ischemia, the impaired degree of my ocardial contractile function is similar to that in stunning group, suggesting a t this stage the effect of ischemia on myocardial function is greater than that of calcium overload. Tetrandrine chronically improves the myocardial function in Vitamin D3-induced calcium overload rats.

Purpose To study the clinicopathologic features of inflammatory myofibroblastic tumor (IMT) of long bone.Methods HE and immunohistochemistry of EnVision two-step were used to observe the clinical,radiological,histological and immunophenotype features of IMT of bone.The literatures were reviewed.Results 4 cases of IMT of bone were respectively located in the tibia (2 cases) and femur (2 cases).Histologically,the lesions were characterized by collagen-rich and spindled to plump myofibroblast-like cells and a variable admixture of inflammatory cells.Immunohistochemical study showed that the vimentin,SMA,actin,H-caldesmon and CD34 were positive.Conclusion The IMT is a rare and locally aggressive tumor.The diagnosis should combine the histological characters with immunohistochemical results and should be differentiated from the other tumors and tumor-like lesions.