Over the years,cerebral vasospasm has been regarded as one of the important factors influencing the prognosis in patients with subarachnoid hemorrhage.Studies have shown that cerebral vasospasm is associated with nitric oxide and nitric oxide synthase after subarachnoid hemorrhage,and regulating the synthesis of nitric oxide may play an active role in the treatment of cerebral vasospasm.

Objective To discuss clinical factors associated with high serum bilirubin level postoperatively in patients with liver cancer. Methods In this study,130 postoperative cases of primary liver cancer were divided into two groups of high serum bilirubin and of normal bilirubin according to serum bilirubin level during two weeks of postoperation. Results Preoperative serum bilirubin、 Child grade、 operation time、 blood loss and types of hepatic vascular exclusion were correlated with the level of postoperative serum bilirubin. Types of hepatic vascular exclusion was a independent factor predicting postoperative elevation of serum bilirubin level. Conclusions Preoperative liver function, operation time, types of hepatic vascular exclusion and blood loss are all among factors influencing postoperative serum bilirubin level.

Recently,the techniques for proteomics have made remarkable progress.They are widely used in various fields of life sciences,providing strong technical supports for relevant researches.Especially,proteomics technology has made prominent contributions to drug development and mechanism studies,magnificently improving the efficiency of discovering new drugs.This paper summarizes the classical method and new technology of proteomics.Also,its applications in drug study,including targets screening,mechanism studies,drug toxicology,researches of resistance mechanisms and clinical medical studies have been reviewed.

Objective To investigate myostatin gene mRNA expression in the muscle tissue from patients with muscle weakness suffering from different illness.Methods The clinical data of our patients were all from the Muscular Disease Center,Department of Neurology,People' s Liberation Army General Hospital.A total of 75 patients suffered from muscular weakness were included consecutively.Skeletal muscle biopsies were obtained with informed consent from all 75 patients.The diagnosis was confirmed by two senior doctors for muscular disease according to the clinical feature,the results of electromyography,serum creatine kinase activity and histopathological examination.Among of them,21 cases were diagnosed as polymyositis,15 cases progressive muscular dystrophy,5 cases neurogenic amyotrophy,4 cases chronic muscle fiber damage,4 cases mitochondrial myopathy,4 cases lipid storage myopathy,4 cases myotonic dystrophy,3 cases muscular dystrophy in adults,2 cases dermatomyositis,and 2 cases inclusion body myositis.There were 2 cases characterized by pure high activity of creatine kinase.And the other 9 cases were diagnosed as non-neuromuscular disease.The expression of myostatin gene mRNA in muscle tissue was evaluated by reverse transcription polymerase chain reaction method,with glyceraldehyde-3-phosphate dehydrogenase as internal reference.Results The expression of myostatin gene mRNA was detected in 63 patients,but not in other 12 cases,and the percentage of positive expression was 84％.The expression index was with great variation,from 0 to 3.52.In positive cohort,the index was correlated positively with the duration of disease (r =0.236,P =0.041).The activities of creatine kinase in positive expression cohort were higher than that of negative one,but nonsignificantly.Conclusion The expression of myostatin gene mRNA in muscle tissue may not correlate to the entity of atrophic muscular disease because of its great variation.

In the present study, A fluorescent imaging-based high-throughput screening method was developed for identifying anti-migratory compounds with 96-well Transwell plates. The correlation, precision and stability of this method were examined and the incubation time of dye Hoechst 33342 in addition to migration time was optimized. In addition, The inhibitory activity of anti-cancer drug paclitaxel on tumor cell migration was assayed and an IC50 value of 0.717 micromol x L(-1) was obtained. Using this method, 24 components from Rhizoma Alismatis were screened and one component with anti-migration activity was found. These results show that the new proposed method with good precision, stability and linear range has the potential to assay the inhibitory activity of anticancer compounds.

Objective To study the clinical application s of intravenous myocardial contrast echocardiography (IMCE) in assessing myocardial reperfusion in acute myocardial infarction (AMI) using intravenously infused Levovist. Methods IMCE was performed in 13 AMI patients before and after thrombolytic therapy. The power Doppler signals of the myocardial segments were analyzed and the time-amplitude curves were plotted during Levovist infusion and incremental triggering. The maximal amplitude score (A), the mean ascending slope of the curve(?) and the product of A?? were measured. All patients were followed up for 3 months. And then IMCE indices were estimated for left ventricular function. Results There was a significant increase in A,? and A?? between before and after thrombolysis(P

Objective To explore the value of the square for intracranial puncture orientation applying in the intracranial hematoma microinvasive craniopuncture scavenging technique. Methods Fifty-one patients with hypertensive cerebral hemorrhage were randomly enrolled into the orientation square group( n=27)and the CT orientation routine group (n=24). The puncture point was fixed by certain wayin each group. Both groups received the same intracranial hematoma microinvasive craniopuncture scavenging technique. The punctural precision,the time spended for puncture orientation and the curative effect in two group were observed and compared. Results The deflected rate(18.5%)of the orientation square group was lower than that routine group significantly(50.0%)(P

Recently,increasing cancer researches focus on antibody-drug conjugates(ADCs) which can improve the anti-tumor potency with less adverse effect while benefiting patients in the future. However,safety evaluation of ADCs is a big challenge because of complex components as well as in experience in preclinical studies. In this review,the authors reviewed the mode of action,hazard risks,and toxicity observed in preclinical/clinical studies of ADCs,summarized the preclinical studies of Adcetris(brentuximab vedotin)and Kadcyla(ado-trastuzumab emtansine),and suggested a better strategy of ADCs in preclinical safety evaluation.

Objective To establish the quality standard of Heibu ointment. Method The contents of Galla Halepensis were determined by TLC, and Gallic Acid was determined by HPLC. Results In TLC, the spots were very clear and specific to identify Heibu ointment. The linear range of Gallic Acid was 0.1～1.1 ?g. The quality standard of Heibu ointment was established. Conclusion The method can be used for quality control of Heibu ointment.

Objective To investigate the quality and academic level of Traditional Chinese Drugs Research and Clinical Pharmacology by analyzing the cited articles.Methods Statistic Analysis and evaluation of citation of articles which were issued in Traditional Chinese Drugs Research and Clinical Pharmacology from the year of 2000 to 2004 and cited in the database of Chinese Biomedical Citation Index and the website of China Knowledge Information,was carried out by literature measurement method.Results There were 770 articles issued in Traditional Chinese Drugs Research and Clinical Pharmacology from the year of 2000 to 2004,the citation rate was 52.59 %,and the average citation frequency of each article was 3.22.There were a large amount of articles with high citation rate.The institutions of cited authors covered 27 provinces (including municipalities and autonomous regions ),special zones and countries,in which Guangdong,Beijing and Jiangsu provinces took the leading in the research of traditional Chinese medicine.The journal was cited by other 350 periodicals,and the citation rate was 93.72 %.The self-citation rate was 6.27 %.Conclusion The articles issued in Traditional Chinese Drugs Research and Clinical Pharmacolog have a high quality,and the journal is one of the most important information suppliers for the research of traditional Chinese drugs and clinical pharmacology and also a core journal for the development of new Chinese drugs and for the research of clinical pharmacology.