Objective: To observe the position change of the upper mol ar s and incisors in cases with Angle class I malocclusion and crowding treated by extraction of four first premolars. Methods: 20 patients with An gle class I malocclusion and crowding aged 14-16 years old, 8 male and 12 femal e, were treated by extraction of the four first premolars, and Edgewise applianc e. Two X-ray cephalometry films were taken for every patient before and after t reatment, cephalometry measurements were obtained and the position of the upper molars and incisors were analyzed. Results:The treatment was co mpleted in 18 month on average in all patients. After treatment, the crown of th e upper first molars showed mesial displacement of 3.15 mm on average, the crown of the upper incisors distal displacement of 2.55 mm, the crown of the lowe r first molars mesial displacement of 4.3 mm, the crown of the lower incisors d istal displacement of 1.77 mm. Conclusion: With of dental anchor age, the position changes of the upper molars and incisors in the cases with Ang le class I malocclusion and crowding after extraction treatment may be achived.I n relation to RL line,the mesial displacement of molars are larger than the dist ally displacement of incisors.

Objective To investigate the correlation between adiponectin, CRP and homocysteine (Hcy) and evaluate its mechanism in acute cerebral infarction. Methods Serum adiponectin, hs-CRP,Hcy, blood lipid, FPG and blood pressure were measured in eighty patients with acute cerebral infarction ( cerebral infarction group) and eighty healthy persons ( control group). Results In cerebral infarction group, serum hs-CRP, Hcy, TG , and systolic pressure were significantly higher than in control group ( P ＜0. 05) , while serum adiponectin were significantly lower than in control group ( P ＜0. 05). Serum adiponectin levels were negatively correlated with Hcy( r = -0. 453) , hs-CRP ( r = -0. 397) ,TG( r = -0. 321) , FPG( r = -0. 318) and systolic pressure ( r = -0. 288). Conclusion There is correlation between adiponectin, Hcy, hs-CRP, glucose and lipid metabolism and blood pressure, which plays a role in occurrenc and development of acute cerebral infarction.

Objective To study recent clinical efifcacy and safety analysis of tinidazole and metronidazole in treatment of severe oral infection. Methods, 88 cases with severe oral infection(SOI) collected in the third people's hospital of Wenling from April 2012 to April 2013 were divided into observation group (n=45) and control group (n=43) by random number table. Control group were received metronidazole drug treatment, while observation group were treated with tinidazole medication, The efifcacy, recovery duration and adverse reactions were compared between two groups. Results The efficacy of observation group was significantly better than control group, the difference was statistically significant (P<0.05). The average duration of symptoms and wound healing in observation group were (4.8±0.4)hs and (4.3 ± 0.2)hs, which were signiifcantly less than (5.5 ± 0.2)sh and (5.6 ± 0.1)sh in control group(P<0.05).Adverse reactions in observation group were signiifcantly lower than that in control group (P<0.05). Conclusion Tinidazole has a signiifcant effect and less adverse reactions in treatment of SOI.

Objective To investigate the role of PT H (1-34 ) on the expression of receptor activator factor of NF-κB ligand (RANKL) in the induction of osteoclasts and its effect to osteoclasts on compression side during the repairs of tooth root in rats model of tooth resorption by intermittent injection of small dose of PTH (1-34) .Methods After the model of tooth resorption was established in rats 6-8 weeks in age ,63 male SD rats were divided in three groups .Rats in the control group were not given injec-tions for any drugs ;The negative control group were given injections for normal saline 6μg/kg subcutaneously every other day ;The experimental group were given injections for PTH (1-34) 6 μg/kg(PTH :1 μg/mL) subcutaneously every other day ;then rats in every group were killed on the day 0 ,7 ,10 ,14 ,17 ,21 ,25 .TRAP staining for counting TRAP-positive stained osteoclasts ,calculat-ing the mean ;Ligand RANKL immunohistochemistry and using image-pro-plus image analysis system to measure the average opti-cal density value of compression side .Results On the day of discontinuation ,the tooth resorption continued in each group ;the num-ber of osteoclasts between every two arrays there were no significant statistic differences (P>0 .05);RANKL immunohistochemis-try :Compared with control group and the negative control group ,the experimental group significantly increased in early stage ,and reduced in latest stage(P<0 .05) .Conclusion It indicated that intermittent injection of small dose PTH (1-34) did not cease the rats tooth resorption which occured during the period of tooth repair .

Condyloma acuminatum is closely associated with human papillomavirus (HPV). In this study the method of slotting hybridization was used to detect the Specimens of 28 cases of condyloma acuminatum. Fourteen out of the 28 samples were positive for HPV 11 probe, the positive rate was 50% (14/28). Nine out of the 14 positive samples were digested with Bamll Ⅰ and Pst Ⅰ respectively to study the present state of DNA after using Southern blotting hybridization. It was shown that HPV 11 DNA were mainly present in a simple monometric episomal state in the condyloma acuminatum cells.

The transformation of human papillomavirus type 16 (HPV16) may play somc important roles in the oncogenesis of cervical carcinoma Studies showed that the transforming gence mainly located in the E6. E7 open reading frames (ORFs). We digested HPV16 DNA with PstI+EcoRI. Using pUC-19 as the vector, E. coli JM103 strain as the host cell, after two times of directive subelone, plasmid pEP-8 was construeted, confirmed by RE analysis and Southern blot hybridization, the inserted band of pEP-8 was 1.43Kb long contained the intact E6 E7 ORFs, and also a cat-box region, two TATA-boxics and a cell-type specific cnhancer in the upstream swquence. The pEP-8 could be used as the specific probe of HPV-16 E region for identifing virol mRNAs in the HPV infected cells. Meanwhile it was a basework for studying the transformation and transforming proteins of HPV-16.

Objective To define the role of HCV-specific cytotoxic T lymphocytes (CTL) in chro-nic hepatitis C virus (HCV) infection. Methods HCV-specific CTL activity (HCV-CTL) was assessed in the liver and peripheral blood cells in 62 patients with chronic hepatitis C and 8 non-HCV-infection controls by using bulk-expanded liver/peripheral blood mononuclear lymphocytes (PBMC)-derived CD8+ cells as effector cells, EBV-transformed B cells as autologous target cells and recombinant vaccinia vectors expressing various regions of the HCV genome as transduction vector, in a standard chromium release assay. Results HCV-CTL activity was detected from the liver in 28 of the 60 patients (46.7%), but not from PBMC. CTL activity could not be detected from the liver and PBMC in all non-HCV-infection controls. Five patients with non-type 1 HCV infection were found to have HCV-specific CTL activity against HCV type 1 epitope. Compared with the patients without detectable HCV-specific CTL activity based on our assay, those exhibiting CTL activity had higher serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, higher histologic activity indices and lower levels of HCV RNA (all P

OBJECTIVEThis study aims to analyze the long-term stability of the anterior teeth and hard tissue of skeletal class III malocclusion after a three-year orthodontic surgery by systematic review.

METHODSAll studies about skeletal class III malocclusion with orthodontic-surgery were searched by computer-based retrieval and manual retrieval; the deadline is December 2013. The literature, filtered according to the inclusion criteria and exclusion criteria, was performed with quality. assessment. The same indicators of the anterior location and hard tissue stability were combined and evaluated with metaanalysis and descriptive analysis by Rev Man5.2.

RESULTSFour before-and-after comparison study articles with 180 cases were included. The grades of the four literature evaluation were A. The meta-analysis results showed that comparing the three-year post-orthodontic-surgery and post-orthodontic-surgery, the total weighted mean difference (WMD) of Ul-SN was 4.29 (P<0.05); the WMD of Ll-MP, OB, OJ, SNA, SNB, ANB, and MP-SN were -1.58, 0, -0.41, -0.58, 0.25, -0.70, and 0.39, respectively (P>0.05). The measurement methods of A and B point position were different, hence the qualitative description were as follows: point A remained at a relatively stable position, and point B had some replacement compared with post-operative (P<0.05).

CONCLUSIONTo the skeletal class III malocclusion after three-year orthodontic-surgery, the position of the lower anterior teeth could be kept stable, as well as the overbite and the overjet of the anterior teeth; only the upper inci- sor has a lip-inclined relapse. The maxillary could also be kept stable, and the mandibular had a little relapse.

Objective To observe the pharmacological effect of Jiaweisinisan (JWSNS) on alcohol liver diseases. Methods Feeding Wistar rats with alcohol induced the alcohol liver diseases model. Then JWSNS was used on the alcohol liver diseases model to observe the histological variation of liver and detect the effect on the liver’s expression of SOD, GSH-PX, CAT and MDA. At the same time, Xiaochaihu granule group was used as control. Results On the alcohol liver diseases model group processed with JWSNS, obvious decrease of pathological injury with expression of MDA in liver was observed, while a markedly increased expression of SOD, GSH-PX and CAT was detected. Conclusions JWSNS can prevent liver cells from peroxided injury by alcohol.

Objective To observe the clinical effect of Jiawei Sinisan (JWSNS) on patients with alcohol liver diseases. Methods 80 patients of alcohol liver diseases were divided into two groups, JWSNS and Xiaochaihu (as control) were used respectively to observe the variation of lipoprotein and the expression of hepatic fibrosis markers. Results The symptoms and indicators of patients were improved by JWSNS. The high variation of lipoprotein and the expression of hepatic fibrosis markers were obviously detected on alcohol liver diseases patients who were treated with JWSNS (with an effectivity of 97.5%). However, patients treated with Xiaochaihu had less therapeutic effect. Conclusions JWSNS can obviously regulate the fat metabolism, resist hepatic fibrosis, and improve the symptom of patients suffering from alcohol liver diseases.