Objective:To investigate the effect of pravastatin on oxidative stress and renal function in the kidney of diabetic rats.Methods: SD rats were randomly divided into normal control group(CN),diabetic control group(DM),pravastatin treatment group(DM+P) and melatonin treatment group(DM+M).Pravastatin or melatonin was given to streptozocin-induced diabetic rat for 8 weeks,then the general state and renal function of rats were evaluated.The serum and renal intrinsic anti-oxidant enzyme(AOE) activities and lipid peroxide levels were also examined.Results: Diabetic rat model was successfully induced.Compared with DM control group,the urinary protein excretion was lower in DM+M group((P

OBJECTIVE:To prepare Compound xianling-branded transdermal ointment and to discuss its pharmacologi-cal action.METHODS:The Chinese crude drugs in the formula were extracted by distilled water boiling and alcohol precipi-tation method,then the extracted essential oils were dissolved in azone,and then mixed with O/W cream-base and drug ex-tracts to prepare the end product.Furthermore,the comparative experiment was made in mus musulus albus to test the effects of the ointment on strengthening of masculinity(anti-fatigue cold endurance,and weight gain)in mus musulus albus,mating ability in male mus musulus albus,and weight gain of sex organs in young male mus musulus albus.RESULTS:The prepared product was light brown in appearance,smooth and delicate in quality,suitable in density,felt good and was easy to spread and clean.Besides,the preparation was stable in action.The preparation could strengthen masculinity and mating ability in male mus musulus albus,and increase the weight of sex organs in young male mus musulus albus.CONCLUSION:The preparation has confirmed therapeutic effect on impotence.

Aim To investigate whether acteoside can protect the dopaminergic(neurons,)(SH-SY5Y)(cells,) from rotenone-induced apoptosis.Methods Cell viability was analyzed by MTT assay.The neurons were stained with the fluorescent dye,Hoechst 33342,to analyze the nuclear change.Flow cytometry was used to determine neuronal apoptotic peak quantitatively.The level of intracellular reactive oxygen species(ROS) was monitored using the fluorescent probe 2′,7′-dichlorofluorescin-diacetate(DCFH-DA).Results ①SH-SY5Y cells treated with 0.5 ?mol ?L~(-1) rotenone for 48 h had a significantly decrease on cell viability compared with control,and the percentage of apoptosis was increased to 47.39%.The cell bodies of most cells shrinked and the dendrites of most cells became shorten or broken.Meanwhile,the shrinkage,condensation and cleavage of nuclei in most cells could be observed.The level of intracellular oxygen species was enhanced.② The preincubation with acteoside(10,20 or 40 mg?L~(-1)),one of the phenylethanoids isolated from Cistanche salsa,for 6 h enhanced the cell viability,and improved the rotenone-induced cell morphological change.Moreover,the percentage of apoptosis was significantly decreased to 25.87%,23.97% and 10.45% in a dose-dependent manner.The enhancement of ROS induced by rotenone was inhibited by 20 mg?L~(-1) acteoside.Conclusion These results demonstrate that acteoside can protect SH-SYSY cells against rotenone-induced apoptosis.The neuroprotective effect might be related with the function that acteoside can reduce the level of ROS.

Objective To investigate the improvement role of low-dose aspirin combined with metoprolol in high blood coagulation and cardiac function of elderly patients with heart failure. Methods 112 cases of elderly patients with heart failure who were treated in our hospital from February 2014 to February 2016 were selected, these patients were randomly divided into conventional therapy group and combined treatment group (conventional therapy combined with low-dose aspirin beauty metoprolol treatment group) two groups, 56 cases in each group. Conventional therapy group was given routine treatment, combined treatment group wered treated on the basis of conventional therapy combined with metoprolol in the treatment of low-dose of aspirin. The plasma P-selectin, VWF, DD, BNP levels, HR, LVEDV, LVEF and clinical efficacy of the two groups were statistically analyzed. Results The P-selectin, VWF, DD, BNP levels of the combined treatment group were significantly lower than those in the conventional treatment group, the difference was statistically significant (P<0.05), the total treatment effective rate 89.3% (50/56) was significantly higher than the conventional therapy group 78.6% (44/56), the difference was statistically significant (P<0.05), but there was no significant difference between the two groups in HR, LVEDV, and LVEF. Conclusion Low-dose aspirin combined with metoprolol can improve the high blood coagulation and cardiac function of elderly patients with heart failure.

Object To study the apoptosis induced by sodium artesunate (SA) in human liver cancer cells and its molecular mechanism. Methods Induction of the apoptosis was analyzed by fluorescent microscope, transmission electron micrograph and flow lytometry based on drug treated BEL-7402. Expressions of p53, p21 and bc1-2 were determined by Western blot. Results The expression level of p53 and p21 had no change remarkbly, but the expression level of bc1-2 was reduced compared to that of the control group after the human liver cancer cells were treated by SA. Conclusion The apoptosis in human liver cancer cells (BEL-7402) may be induced by SA, the molecular mechanism of apoptosis may be p53-independent, that is no relative to p53 and p21. Otherwise, it is relative to bc1-2 down-regulation.

Breast cancer is one of the most common malignant tumor for women, and early and accurate diagnosis of the breast diseases may improve the therapy and prognosis. MRI gains advantages in the diagnosis of the breast diseases, and thus is an important tool for the imaging diagnosis of the breast. The examination modes of MRI were introduced for the diagnosis of the breast cancer, especially the application in high risk population. The limit and research progress of MRI were analyzed for breast cancer staging, pre-operative examination, therapeutic effect assessment for radiotherapy and chemotherapy, the relationship between the markers and imaging findings.

Proteomics has been applied in a wide range of biomedical research.However,the application of proteomics in studying the molecular mechanism of morphine dependence is only at a preliminary stage.This article introduced the application of proteomics techniques in the study of the molecular mechanism of morphine dependence and the discovery of several potential molecular markers of morphine dependence,which affirmed the importance and potential of proteomics in this research area.Also,it was pointed out that the major tasks of current proteomic study of morphine dependence should include establishing animal and cell models of morphine dependence,selecting appropriate sample source and improving proteomics techniques,so that proteomics can serve as a new approach in the study of morphine dependence to discover new therapeutic targets.

Objective,To evaluate the value of magnetic resonance imaging(MRI) in clinical diagnosis of tuberculous meningitis.Methods,Forty-six patients (29 men,17 women,age ranged 11-72,mean age 41.5) with tuberculous meningitis were examined with MRI.The MRI findings of tuberculous meningitis were analyzed retrospectively.Results,On pre-contrast T1WI and T2WI,meningeal lesions in the basal cistern region were detected in twenty cases.Abnormal meningeal thickening and enhancement were found in 38 patients.Meningeal and cerebral miliary tuberculous foci were demonstrated in 21 cases.Ependymal involvement was found in six patients and cranial nerves were affected in other two patients.No abnormal meningeal enhancement was detected in eight mild cases.On the follow-up images in nine patients,the abnormal findings alleviated in varied degree or,remained unchanged.Conclusion,The pathological changes in tuberculous meningitis could be demonstrated effectively by specific findings on MRI.MRI,especially the post-contrast MRI,proved a reliable modality in clinical diagnosis and assessment of tuberculous meningitis.

Objective To investigate the changes in 20S proteasome activities in the brain and spinal cord of acute and chronic morphine-dependent mice.Metbods Male ICR mice,weighing 25-30 g,were used in the study.The experiment was performed in 2 parts.In experiment Ⅰ,16 mice were randomly divided into 5 groups (n =8 each) using a random number table:control group (group C) and acute morphine dependence group (AMD group).In experiment Ⅱ,16 mice were randomly divided into 5 groups (n =8 each) using a random number table:control group (group C) and chronic morphine dependence group (CMD group).Acute morphine dependence was induced with morphine 100 mg/kg injected subcutaneously,and the mice were sacrificed 3 h later.Chronic morphine dependence was induced by increasing doses of morphine for 4 days,the initial dose of morphine was 20 mg/kg injected subcutaneously twice a day and was increased by 10 mg/kg every day,the dose of morphine was 10 mg/kg injected subcutaneously on 5th day,and then the mice were sacrificed 1 h later.In group C,the equal volume of normal saline was given instead,and the other treatments were similar to those previously described in morphine dependence groups.After the mice were sacrificed,the hippocampus,prefrontal cortex,striatum and spinalcord were isolated for determination of 20S proteasome activity,measured as chymotrypsin-like (ChT-L),trypsin-like (T-L) and peptidylglutamyl-like hydrolyzing (PGLH) activities.Results Experiment Ⅰ Compared with C group,PGLH activity in the spinal cord and T-L activity in the striatum or prefrontal cortex were significantly weakened in group AMD.There was no significant difference in 20S proteasome activity in the hippocampus between the two groups.Experiment Ⅱ Compared with C group,ChT-L and T-L activities in the spinal cord were significantly weakened,and PGLH activity in the striatum was enhanced in CMD group.There was no significant difference in 20S proteasome activity in the prefrontal cortex and hippocampus between the two groups.Conclusion 20S proteasome activity in the spinal cord and brain is weakened in acute morphine-dependent mice,20S proteasome activity in the spinal cord is weakened,20S proteasome activity in the striatum is enhanced in chronic morphine-dependent mice,these changes have specificity in terms of position and type of activity,and the changes mentioned above may be related to development of morphine dependence in mice.

Objective To evaluate 5 drug sensitivity tests in detecting carbapenem resistance of KPC-possessing Enterobacteriaceae.Methods Thirty-six Enterobacteriaceae isolates were collected from the First Affiliated Hospital,Cofiege of Medicine,Zhejiang Univeristy,including 32 of Klebsiella pneumoniae,1 of Escherichia coli,1 of Citrobacter freundii,1 of Klebsiella oxytoca,and 1 of Serratia marcescens,and the cacbapenemase producing was confirmed with PCR.Resistance to imipenem,meropenem and ertapenem was determined by agar dilution and disk diffusion tests,that to imipenem and meropehem was determined by ATB with G-5 panel,that to imipenem by BD Phoenix 100 with NMIC-109 panel,and that to imipenem and ertapenem by VITEK 2 Compact with AST-GN13 card.Results The resistance to imipenem determined by ATB,BD Phoenix100,VITEK 2 Compact,agar dilution and disk diffusion tests were 13.9%(5/36),11.1%(4/36),13.9%(5/36),22.2%(8/36)and 69.4%(25/36).ATB,agar dilution and disk diffusion tests reported 22.2%(8/36).55.6%(20/36)and 47.2%(17/36)as resistant to meropenem.VITEK 2 Compact,agar dilution and disk diflusion tests reported 69.4%(25/36),77.8%(28/36)and 88.9%(32/36)as resistant to ertapenem.The MICs of ertapenem determined by VITEK 2 Compact and agar dilution were≥2 μg/mL.Conclusion The sensitivity of agar dilution and agar disk diffusion is higher than other automatic methods in the identification of KPC-mediated carbapenem resistance,and ertapenem is a more sensitive indicator than mempenem and imipenem for screening KPC-possessing isolates.