Objective To understand the distribution characteristics of HIV infection status among the outpatients in this hospi-tal to provide the beneficial supplement for local large-scale survey and the basis especially for the hospital infection control.Meth-ods The HIV screening situation on the outpatients of our hospital from 2007 to 2012 was retrospectively analyzed.Results Dur-ing these 6 years,108 999 specimens were detected,88 cases were positive in the preliminary screening and 81 cases were confirmed positive.The positive rate of anti-HIV antibody was 0.074%.In addition to voluntary counseling and testing,the specimens submit-ted by the outpatient anorectal department and the inpatient hematology department had the higher positive detection rate,which was 1.16% and 0.84% respectively.Conclusion The HIV infection rate in non-infectious disease specialized hospitals shows the increasing trend.The key departments must increase the intensity of the medical staff training,pay attention to early diagnosis of HIV/AIDS,prevent the hospital infection and reduce the risk occurred after the occupation exposure.

Objective To discuss the efficacy ofcimetidine combined with acyclovir in treatment of children with varicella.Methods 80 cases ofvaricella were randomly divided into two groups.The observation group (40 cases) was treated with cimetidine combined with acyclovir treatment.The control group (40 cases) was given the acyclovir.Observe and record the two groups of children with clinical efficacy,fever time,rash scab,itching disappeared time after treatment,before and after treatment during the HAMA score and the treatment of adverse reactions.The clinical curative effect of cimetidine combined with acyclovir in treatment of children with varicella was evaluated.Results The observation group of total effective rate was 87.5％.The control group of total effective rate was 65％.The total effective rate of observation group was higher than the control group,the difference was statistically significant (P ＜ 0.05).After treatment,the fever time,skin rash,scab time and pruritus disappeared in the observation group were shorter than those in the control group,the difference was statistically significant (P ＜ 0.05).Before treatment,there was no statistical difference in HAMA scores between the two groups.After treatment,the scores of HAMA in the two groups were significantly lower than those in the same group before and after treatment (P ＜ 0.05),and the treatment group was significantly lower than the control group,the difference was statistically significant (P ＜ 0.05).There was no significant difference in adverse reactions between the two groups during the treatment.Conclusion Cimetidine combined with acyclovir have a good therapeutic effect on varicella.They can rapidly improve the clinical symptoms of pediatric varicella,reduce anxiety with high safety.It is worthy of clinical use.

BACKGROUND:As a material to repair osteochondral defect,the acellular cartilage matdx has attracted more and more researchers'attention,however,there is no comparison between two ways of decelluladzation.OBJECTIVE:To test trypsin and Triton X-100 methods for their potential of cell removal.DESIGN,TIME AND SETTING:Controlled observation was performed at the Central Laboratory of Orthopaedics in the Second Hospital of Shanxi Medical University between March and July in 2008.MATERIALS:Fresh knee and hip articular cartilages of pig were purchased from the market.METHODS:Porcine articular cartilages were treated with either 0.25%trypsin or 0.25% Triton X-100 respectively for decelluladzation,while fresh untreated articular cartilage served as control.MAIN OUTCOME MEASURES:①General observation on the morphology of acellular articular cartilage.②Immunohistochemistry and scanning electron microscopy were used to determine the effect of two decellularization methods on the extracellular matrix collagen.③Ten articular cartilage leaflets in each group were measured for breaking strength and percentage elongation through loading,unloading and breaking tests.RESULTS:①By use of trypsin and Triton X-100 methods,the acellular matrix had no significance difference in the morphology compared with untreated cartilage,only presenting slightly white color and feeling less soft.②lmmunohistochemistry and scanning electron microscopy results showed that,trypsin and Triton X-100 methods achieved complete decelluladzation.Trypsin had no significant influence in extracellular matrix and cartilage leaflets microstructure;Triton X-100 caused some tiny structural alterations,such as more collagen and disordered structure.It is possibly that Triton X-100 damaged extracellular matrix.③No significant difference was identified between untreated and trypsin,Triton X-100 groups in breaking strength and percentage elongation.CONCLUSION:Trypsin and Triton X-100 all achieve complete decellularization.but trypsin uses short time and low costs with well preservation,while Triton X-100 with long procedure and slight damage to collagen structure.They all have no significance influence to the mechanics characteristics of articular cartilage.

Objective:To observe clinical therapeutic effect of Fu Fang Qi Dan Dai Zhu San on sequelae of cerebral hemorrhage. Methods: 56 cases were selected randomly from the 315 cases who had received functional exercise, massage and other rehabilitation treatments. The patient were administrated by Fu Fang Qi Dan Dai Zhu San Decoction for 3 months, which is constituted by Bu Yang Huan Wu Decoction and Fu Fang Dan Shen Tablets, and the therapeutic effect was compared with that of Bu Yang Huan Wu Decoction, Fu Fang Dan Shen Tablets and other clinically commomly - used drugs, respectively. Results: Both the clinically cured rate and the total markedly effective rate in the treatment group of Fu Fang Qi Dan Dai Zhu San were significantly higher than those of the six control groups. Conclusion: Fu Fang Qi Dan Dain Zhu San can obviously increase the therapeutic effect and shorten the therapeutic course, and it is a good prescription for treatment of sequelae of cerebral hemorrhage.

Abstract To analyse the mechanism of TECA bio-artificial liver support system(BALSS)to treat acute liver failure (ALF) canines. The blood of partial liver resection-induced ALF canines or RPMI1640 solution were circulated in the inner space of the hollow fibers (blood loop), and porcine hepatocytes were cultured in outer space of the hollow fibers (cell loop). The concentrations of lidocaine, albumine and urine in the RPMI1640 solution were measured. The compositions of proteins in cell loop and blood loop were analysed by polyacrylamide gel electrophoresis (PAGE). The ultrastructure of porcine hepatocytes in BALSS was observed with transmission electronic microscopy. There were not any proteins in RPMI1640 solution before BALSS circulation. After 6 hours of the BALSS circulation, the concentration of lidocaine in RPMI1640 solution decreased significantly,and concentration of albumine and urine nitrogen increased gradually. The ultrastructure of the porcine hepatocyte shown damaged after BALSS 6h. The results suggested that TECA BALSS could replace temporarily the functions of failure liver by the effects of biologic transform and synthesis of porcine hepatocytes.

Objective To investigate the relationship between air pollutant exposure and preterm delivery (PTD) by Logistic regression model, as the effect of air pollutant exposure to PTD made great concern today. Methods The present study consisted of 31 145 qualified birth cases, 80.09% of the total amount, from 1 Nov, 2005 to 1 Aug, 2007. The covariant items included maternal age, nation, education, residence, income, occupation, occupation exposure, parent-drinking, father-smoking, heating-type, folic acid supplement, season of pregnancy, prenatal health care, etc. The Logistic regression analysis was employed to control the confounding factors mentioned above, then the pollutant was introduced step by step to build the single, double and triple pollutant Logistic regression model to examine the association between three pollutants (SO2, NO2, and PM10) and PTD during the first and third trimesters of pregnancy. The air monitoring data (daily average of SO2, NO2, and PM10) were collected from Taiyuan Environmental Monitoring Station. Results In the first trimester, a quartile increase of NO2 exposure caused the risk for PTD increased by 22.7%. As for the effect of PM10, no significant differences were seen. In the third trimester, a quartile increase of NO2 exposure caused the risk for PTD increased by 19.1%. Conclusion The NO2 exposure during the pregnancy can increase the risk of PTD. The susceptible exposure-window choice is critical for the further study in this field.

OBJECTIVE:To improve the quality of pharmaceutical care and to provide bases for outpatients and medical staff in drug informatin inquiry.METHODS:Information about drug package inserts wre collected and the drug-inquiry system was designed using the database management function of Access.RESULTS & CONCLUSIONS:The drug-inquiry system is easy to operate,clear in interface,humanization in operation,and it faciliats individualized drug information inquiry through daily mantinance and satisfies the requirments for outpatient drug information inquiry.

Objective:To probe the application and the effect of integration medical health education mode in the care-givers of children with capillary bronchitis. Method:Total 120 cases of capillary bronchitis caregivers were divided into control group (56 cases) and observation group (64 cases) and control group with traditional mode of health education, observing group adopts the integration medical health education mode;Compared two groups of children with hospitaliza-tion days, take care of health education knowledge of cooperation degree, satisfaction and health care work. Results:After implementation the integration medical health education mode, children hospitalization days declined. Caregivers' health education knowledge and satisfaction were obviously improved. The medical work cooperation degree is higher than the control group. Conclusions:The implementation of the new mode is helpful to improve work efficiency, reduce the finan-cial burden on families, and meet the demand of caregivers, health education and the right to know and sublimation of medical humanism philosophy, promoting the comprehensive qualities of nurses, improve patient satisfaction, harmonious relationship between hospital and patients, improve high quality nursing care for patients.

Objective To investigate the clinical characteristics and risk factors for healthcare-associated infection (HAI)in patients with severe chronic hepatitis B (CHB),and provide theoretical basis for preventing and controlling HAI.Methods Retrospective survey was used to investigate the occurrence of HAI in hospitalized patients with severe CHB in a hospital between January 2012 and January 2015,risk factors for HAI were analyzed. Results A total of 126 patients with severe CHB were investigated,49 patients developed 106 times of HAI, incidence of HAI was 38.89%.The main HAI site was respiratory tract (n=47,44.34%),the next was abdominal cavity (n=34,32.08%).A total of 76 isolates of pathogens were detected,gram-negative bacteria,gram-positive bacteria,and fungi accounted for 53.95%(n =41 ),43.42%(n =33),and 2.63%(n =2)respectively.Risk factors for HAI in patients with severe CHB were patients ’ age ≥ 60 years, length of hospital stay ≥ 30 days, complications,invasive operation,serum albumin < 35 g/L,and white blood cell count (WBC)< 4 × 109/L. Conclusion Incidence of HAI in patients with severe CHB is high,the majority are respiratory tract and abdominal cavity infection,risk factors are old age,long length of hospital stay,invasive operation,hypoalbuminemia,and low WBC count.

Aim To express hepatitis C virus glycoprotein E (gE) deleting carboxy-terminal 31 amino acids, and detect anti-E antibody in HCV patients using expressed gE. Methods E gene derived from HCV H strain was inserted into baculovirus transfer vector containing a polyhedrin promotor. The recombinant plasmid was cotransfected into insect cell sf9 with a viral expression vector. The expression of gE was analyzed with Western blot, and the cells were used for dectecting antibodies against E1 and E2 in 35 hepatitis C patients by indirect immunofluorescence. Results A series of proteins with different relative molecular masses(Mr) could be detected by Western blot. Results from indirect immunofluorescence staining showed and only 4 patients were anti-E antibody positive gE was located in cytoplasm. Conclusion HCV gE is expressed successfully in insect cells, the study lay the foundation for further developing HCV vaccine.