OBJECTIVE: To study the effects of Folium Crataegi water extract on contractility of isolated gastric and intestinal smooth muscle strips in rats. METHODS: The effects of Folium Crataegi water extract on the contractility of isolated rat gastric and intestinal smooth muscle strips in the presence of normal Krebs’ solution, acetylcholine or atropine were investigated. RESULTS:Folium Crataegi water extract (5～20 mg?mL-1)significantly enhanced the contractility of rat gastric and intestinal smooth muscle strips in a dose-dependent manner, and Folium Crataegi water extract (20 mg?mL-1) could enhance the intensive contraction induced by acetylcholine and antagonize the relaxation of intestinal smooth muscle induced by atropine. CONCLUSION:Folium Crataegi water extract has remarkable stimulation on the contractility of isolated rat gastric and intestinal smooth muscle strips.

0.05). Conclusion Both CD54 and LRP expression have negative correlation with the effective rate of short-term therapeutic effects, therefore can be taken as indexes for prognostic evaluation for patients with non-small cell lung cancer.

Objective To understand the morphology of supraorbital ridge and toprovide basis for aesthetic surgery. Methods Forehead morphology was examined in 40 adults (20 men and 20 women). By multi-slice helical CT, a computer reconstruction technique was used to produce three dimensional images and 3-D reconstruction data were analyzed quantitatively. Results Ft,-Ft, V N, N-Op, Fb-Sr, Zm-So, Dbr, Pra-N in male were significantly larger than those in female, and V-N, N-Po, Fb-Sr, FA in youth men were significantly smaller than those in old men. Conclusion There is a difference of forehead between male and female. Projection of supraorbital ridge increases with age.

Objective To explore the relationships between serum levels of adiponectin and essential hypertension in the people with overweight and obesity.Methods The body mass index(BMI),waist circumference(WC),waist-hip ratio(WHR) and blood pressure(BP) were measured among 21578 people from 21 to 60 years old in Shenzhen.According to BMI,the people were divided into the normal-weight group(BMI≤24kg/m~2)(n=14817),the overweight group(2495 cm,respectively(all P0.05).The serum levels of adiponectin were negatively correlated with BMI,WC,WHR and systolic blood pressure in the hypertension obesity cases.Conclusion Adiponectin may be intimately related to obesity and essential hypertension.Keeping the BMI and WC at normal range is one of the effective ways to prevent the risk of essential hypertension.

Objective:To investigate the clinicopathological factors affecting the prognosis of colorectal cancer patients with liver metastasis and how to select therapeutic method. Methods:The clinical records of 146 colorectal cancer patients with liver metastases who were admitted in Cancer Hospital of Guizhou Province from March 1997 to March 2007 were collected and made a retrospective analysis. The survival rate of the 146 patients was calculated by using life table method. Kaplan-Meier method and log-rank test were used for univariate analysis on clinicopathological features and therapeutic modalities. The multivariate analysis was performed by using COX regression model. The prognostic index (PI) of patients was calculated based on the result of multivariate analysis. The patients were classified into different risk groups according to PI value and the survival rate was compared between the different groups. Rusults:The overall 1-, 3-, and 5-year survival rates were 62.0%, 15.5%and 6.2%, respectively. Univariate analysis revealed that the following factors were related with the prognosis of colorectal cancer patients with liver metastasis. They included pathological classification, histological grade, serum CEA(carcinoembryonic antigen)level, primary tumor resection, local lymph node metastasis, number and size of liver metastases, distribution and initiation time of liver metastases, extrahepatic invasion, with or without surgery and che-motherapy for liver metastasis, and chemotherapeutic regimen selection. Multivariate analysis showed that the serum CEA level, extrahepatic metastasis, number and size of liver metastases, primary tumor resection, and chemotherapeutic regimen were independent prognostic factors for colorectal patients with liver metastasis.Conclusion:The therapeutic modality had an obvious effect on the prognosis of colorectal cancer with liver metastasis. Active treatment for primary tumor and metastatic lesions increased the survival rate of patients. Serum CEA levels, with or without extrahepatic metastases, and the number and size of liver metastases were prognostic factors. PI value could be used to predict the prognosis of colorectal cancer patients with liver metastasis.

Diabetic peripheral neuropathy is one of the common chronic complications of diabetes, and TCM has unique advantages in the treatment of diabetic peripheral neuropathy. The article summarized the experimental research progress in the TCM intervention in treatment of diabetic peripheral neuropathy in recent years from the aspects of oxidative stress, metabolic disorders, neurological nutrition factor and neural microvascular dysfunction, with a purpose to provide better efficacy in clinical treatment.

Aim To explore the effects and mechanism of ginsenoside-Rg1 on SK-N-SH cells treated with chronic morphine and naloxone-precipitated withdrawal. Methods Cells were pretreated with ginsenoside-Rg1 1,2,4,8,16,32 ?mol?L-1 for 24 h,then incubated for 24 h with morphine ( 100 ?mol?L-1 ) . MTT colorimetr was used to study the effects of ginsenoside-Rg1 on the multiplication of the cells treated with chro-nic morphine. After stimulated by the same concentra-tion of morphine,cells were added with different concentrations of Rg1 1,2,4 ?mol?L -1 for 24 h before stimulated with 10 ?mol?L -1 NAL. Fuorospectrophotometry RT-PCR and Western blot techniques were used to detect the effects of ginsenoside-Rg1 on the [Ca2+ ]i,CaMKⅡ ? mRNA and protein expression of the SK-N-SH cells treated with chronic morphine and naloxone-precipitated withdrawal. Results ① Compared with control group,morphine significantly inhibited cell multiplication and resulted in calcium overload,and the expression of CaMKⅡ-? mRNA and protein noticeably increased ( P

Problems in managing laboratory instruments emerges along with the rapid development of laboratory medicine. Full-time instruments manager is positioned in laboratory department, who is in charge of maintaining all instruments. In this way, problems in managing laboratory instruments are solved, so the instruments in laboratory department can be managed in a standard, scientific, institutional and modern way.

Bone mineral density (BMD) of lumbar spines, femoral neck, Ward′s triangle and greater trochanter was lowered, serum calcium, phosphate, alkaline phosphatase and bone Gla-protein levels were raised, and vitamin D receptor (VDR) contents in lymphocyte was lowered in hyperthyroid patients. Both FT 3 and FT 4 were negatively correlated with BMD, suggesting that hyperthyroidism results in high-turnover type of bone loss which causes the abnormalities of bone metabolic indices and VDR content.

Objective To investigate the clinicopathologic features and differential diagnosis of alveolar soft part sarcoma(ASPS).Methods A case of ASPS was studied by HE stain,light microscopic observation,histochemical stain and immunohistochemical stain,and discussed the disease synthetically with the literature.Results The patient was female,23 years old.The lesion was located in the deep skeletal muscles of left buttocks,microscopically,which consisted of round to polygonal tumor cells,showing alveolus and nest arrangement,arounded by stroma containing sinusoidal capillaries,and there were abundant crystals in cytoplasm of the tumor cells,prominent nucleoli and fewer nuclear fission images in the tumor cells.PAS stain was stongly positive.Immunohistochemical stain showed positive expression of myoglobin in tumor cells,and negative expression of Myosin,Desmin,SMA,Vimentin,S-100 protein,EMA and Syn in them.Conclusion ASPS is a rare malignant soft tissue neoplasm,its histogenesis is uncertain,and diagnosed by pathological examine and treated mainly by operation.