Secondary pancreatic infection in severe a-cute pancreatitis (SAP) was associated with mortality and the length of stay in hospital.Intensive Care Unit (ICU) identificate shock on account of the oxygen metaboilic level in earlier period of SAP and perform adequate fluid resuscita-tion.it advocate to perform organ function support and/or substitution therapy in time and think highly of coordination and combination each other among various kinds therapeutic measure.These therapeutic concept and measure will con-duce to decrease the incidence rate of MODS and secondary pancreatic infection accordingly in SAP.

Objective To study the value of RDW in severity evaluation and prognostic assessment of COPD. Methods Retrospective study was carried out on 115 COPD patients and 50 healthy controls. Blood routine, CRP, IL-6 data were collected on admission aiming at analyzing RDW difference in patients and controls, severity, severity grading and evaluation of prognosis. Results The level of CRP, RDW, IL-6 and WBC in patients were higher than that of healthy controls(P < 0.05). Mild and moderate groups have no significant difference from each other in terms of RDW, CRP and IL-6 conparison, but the difference between any other two groups were significant. There were significant positive correlation between RDW and CRP, IL-6, and negative correlations between the post-bronchodilator FEV1% and RDW. The mortality rate for patients with higher RDW level was higher than those with normal RDW. Conclusion Elevated RDW levels on admission are associated with severity of patients with COPD. RDW may be a prognostic marker for COPD.

At present, the immunological tolerance in transplantation induced by multi-mechanisms, to inhibit the chronic graft rejection, represents an important trend in the field of transplantation. This review summarizes the new researches about immune and non-immune factors in this field. At the same time, this review also discusses that the high-dose vitamin E, through changing the ratio of Th/Ts in vivo and not improving the level of specific-antibody, regulates the immune system. In addition, the potent antioxidation of vitamin E has advantage to release the oxidate press of tissue and the damage of blood vessels. In the end, the reporter prospects the signification of vitamin E to inhibit the chronic graft rejection and points out the potential questions.

PURPOSE To investigate the effects of bcr3/abl2 anisense oligodeoxynucleotide (ASO) on chronic myeloid leukemia (CML). METHODS The effects of bcr3/abl2 ASO in 15 CML patients were evaluated by morphological observation, flow cytometer (FCM) techniques and DNA fragment measurement. RESULTS bcr3/abl2 ASO can induce apoptosis in CML. CONCLUSION Antisense techniques may provide a new way to cure CML.

Objective Preparation of specific antibody of neuron protein 14 3 3 and application for the diagnosis of prion diseases. Methods The rabbits were immunized with prokaryotic expressed GST 14 3 3 fusion protein and the collected antiserum was evaluated by ELISA and Western blot assays. Results ELISA revealed that the reactive titer of the prepared antibody against purified 14 3 3 protein was up to 1:4 096 000; Western blot assays confirmed that both prokaryotic expressed,and the natively presenting 14 3 3 proteins in the brain extracts were recognized by the prepared antibody. Two cases of clinically diagnosed Creutzfeldt Jakob disease (CJD)with typical EEG changes showed the positive 14 3 3 protein in cerebral spinal fluid (CSF). Four patients with dementia and narcoma but without typical EEG change were 14 3 3 negative in CSF,three of them being even further tested by brain biopsies without typical histological change of CJD and presentation of protease resistant PrP Sc protein. 35 samples CFS collected from patients without dementia were 14 3 3 negative in CSF. All the results were the same as that of standard 14 3 3 polyclonal antibody.Conclusion Using purified prokaryotic expressed GST 14 3 3 fusion protein as antigen,the specific antibody was elicited in the immunized animals. The prepared antibody can be used in identification of 14 3 3 protein in CSF for the diagnosis of CJD.

Objective To analyze the clinical data of henoch-schonlein purpura(HSP)in children and to conclude the clinieal featrues of HSP.Methods The clinieal features of 60 children with HSP were analyzed.Results(1)The age of onset of all the 60 children with HSP was from 3 to 14 yearn old,especially from 5 to 10 years and the female patients were more than the male patients(the ratewas 2:1).Of the 60 children,they developed HSP in the four seasons,especially in the spring and winter;most of the HSP patients revealed evident predisposing factors,mainly upper respiratory infection.(2)All the patients had typical purpura.80% had typical purpura as the presenting contancous sign.The distribution of rash in the lower extremitiees,buttock upper extremities and face was 100%,30%,13% and 7%.30% occurre,repeatedly.(3)Gastrointestinal involvement and arthritis occurred rate is 47% and 60%,respectively.Of all the patients,13% devdoped HSP nephritis manifested as isolated hematuria and/or protcinuria.Conclusion HSP has its own clinical and episodic features.It is not difficult to diagnose the typical case.HSPN is its serious syndrome,it should be long-term foflow-up of kidney conditions.

Objective To observe effect of ganciclovir on the treatment of the young child viral encephalitis. Methods 68 cases of viral encephalitis were randomly divided into treatment group(45 cases) and the control group (23 cases), the two groups of children with clinical manifestations and treatment effects were observed. Results Headache, vomiting, convulsions symptoms and the resumption of the time was obviously shorter in treatment group than that of the control group, fever symptoms persisted despite the shortened time, but the difference was not statis- tically significant. Conclusion Ganciclovir in children with viral encephalitis have significant effect.

Objective To investigate the outcomes of arthroscopic treatment of anterior cruciate ligament tibial eminence avul-sion fractures by using loop ligature fixation .Methods 26 patients with anterior cruciate ligament tibial eminence avulsion frac-tures .All the patients were fellowed up at least one year .Results The X-ray indicated that all the avulsion fractures of tibial anteri-or eminence were healed after 6 months of the operation .The mean Lysholm score improved from (50 .8 ± 6 .5) preoperatively to (92 .7 ± 4 .3)postoperatively(t=36 .7 ,P<0 .05) .The mean IKDC 2000 subject score improved from (55 .6 ± 7 .4)preoperatively to (90 .7 ± 4 .2) postoperatively(t=22 .5 ,P<0 .05) .Conclusion Arthroscopic treatment of anterior cruciate ligament tibial eminence avulsion fractures by using loop ligature fixation could provide satisfying reduction ,stable fixation and well healing of the avulsed fragment .This arthroscopic suture fixation method is a minimally invasive ,simple and effective treatment for patients with tibial eminence fracture .

Objective To evaluate the role of oxidative stress in the spinal neurotoxicity induced by ropivacaine in rats.Methods Ninety pathogen-free male Sprague-Dawley rats,weighing 250-320 g,aged 3 months,in which intrathecal catheter was successfully implanted into L5,6 interspace without complications,were randomly divided into 3 groups (n =30 each) using a random number table:control group (group C),ropivacaine group (group R),and antioxidant Tempol group (group T).The rats received 1％ ropivacaine 1.2 μg/g for 8 times at 1.5 h intervals through the catheter in R and T groups,while the rats received the equal volume of normal saline instead in group C.In T group,Tempol 20 μg/g was injected intrathecally at 4,8,12,24,48 and 72 h after the last injection of ropivacaine.The rats were sacrificed at 1,3,5,7 and 14 days after the end of ropivacaine injection,and their lumbar enlargements were removed for TUNEL staining to detect the cell apoptosis.SOD activity was determined by colorimetry and MDA content was measured using TBA photoelectric colorimetry.Apoptosis index was calculated.Results Compared with group C,apoptosis index and MDA content were significantly increased,and SOD activity was decreased in R and T groups.Compared with group R,apoptosis index and MDA content were significantly decreased,and SOD activity was increased in group T.Conclusion Oxidative stress is involved in the development of spinal neurotoxicity induced by ropivacaine in rats.

Objective:To study memory changes in patients with non-demented Parkinson disease(PD) without depression.Methods:The Nissen Version(serial reaction time task,SRTT) software(as a task of procedural learning),the WMS-CR and two tasks of implicit memory were applied in 16 PD patients(Hoehn-Yahr score I~Ⅱdegrees).Normal controls enrolled for the Nissen Version either.Results In the explicit WMS-CR and the implicit(word stem completion and degraded picture naming) tasks,the patients' scores fell within normal limits(Memory Quotient 97.1?10.6).In the SRTT,normal control group displayed significantly reduced response times(F=2.54,P=0.008) and error rates(3.2 ?0.9% to 6.8 ?2.7%,t=-2.08,P=0.045) across the blocks of repeated sequence trials.By contrast,PD patients only showed a reduction in error rates(4.7?2.0% to 3.7?1.8%,t=-2.15,P=0.038) but no change in response times.Conclusion:Impairment of nigrostriatal pathways selectively affects performance in visuo-motor learning tasks such as the SRTT,but not in the explicit tasks of WMS-CR and the implicit tasks such as word stem completion and degraded picture naming.