Over the years,cerebral vasospasm has been regarded as one of the important factors influencing the prognosis in patients with subarachnoid hemorrhage.Studies have shown that cerebral vasospasm is associated with nitric oxide and nitric oxide synthase after subarachnoid hemorrhage,and regulating the synthesis of nitric oxide may play an active role in the treatment of cerebral vasospasm.

AIM: To observe the effects of the combination of positive end expiratory pressure (PEEP) and 80?10 -6 nitric oxide (NO) inhalation on oleic acid induced acute lung injury (ALI) in canine. METHEDS: 30 dogs were divided into 6 groups. Oleic acid was injected through Swan-Ganz catheter to induced ALI. Pulmonary and systemic hemodynamics,blood gas were measured in dogs before and after injection of oleic acid and the period of inhaled NO for 1-6 h. The methemoglobin(MHb) concentrations were measured. Histology and ultrastructure of the lung tissue were observed. RESULTS: (1) The combination of PEEP and 80?10 -6 NO inhalation rapidly reduced mean pulmonary arterial pressure (MPAP) and pulmonary vascular resistance (PVR), increased PaO 2/FiO 2, reduced A-aDO 2 without inducing significant change on systemic hemodynamics. Arterial blood levels of MHb did not change significantly. (2)The combination group was showed the lightest ALI change by HE stain and electron microscopy. CONCLUSION: The combination of PEEP and inhalation of 80?10 -6 NO significantly and rapidly increased PaO 2/FiO 2 without producing lung injury induced by high FiO 2 and high PEEP.

In this paper,the contents of nutrition ingredients and five elements of limit quan- tity in eight species of shellfish in South China Sea were studied. It was found that the contents of Cd was high and Ag,As Cu and Ph were general low in comparison with hygiene criterions.

Objective To evaluate the clinical safety for outpatients over 80 years old after phacoemul sification surgery. Methods The 184 cases (226 eyes) of aged patients with cataracts were operated on with phacoemulsification and IOL implantation. The safety, complications during and after operation, visual recovery and others were followed up over that period. Results All eyes were operated on without any danger and no serious complications were found. The vision acuity was 0 5 or better in 198 cases (87 6%), 1 0 or better in 102 eyes (45 1%). The main complication was corneal edema in 58 eyes(26 2%), rupture of posterior capsule in 12 eyes (5 3%) and posterior capsular opacity in 16 eyes (7 1%). Conclusions The phacoemulsification and IOL for over 80 years outpatients were safe and effective. The key points are systemic physical examination and careful eye examination. Stimulations during operation and postoperative complications should be prevented.

Objective To investigate the association of expressions of cyclooxygenase-2(COX-2),matrix metanoproteinases-9(MMp-9)and Ki67 with occurrence,infiltration and metastasis of gastric cancer.Methods Fifty-eight surgically obtained and pathologically performed cancerous tissues were collected between Jan.2003 and Dec.2005.Among them,37 were males and 21 were females with mean age of 58.2 years(ranged from 31 to 76 years).Fifty-eight paracancerous tissues(5-6 cm apart form the cancer)were taken as controls.The expressions of COX-2,MMP-9 and Ki67 in cancerous or paracancerous tissues were examined by immunohistochemistry.Results The expressions of COX-2 and MMP-9 in cancerous tissues(82.76%and 68.9%,respectively)were higher than those in controls(37.93%and 24.14%,respectively,P＜0.01).The expressions of COX-2 and MMP-9 were not associated with sex,age,location and size of gastric cancer(P＞0.05),but were correlated with the depth of lymph node infiltration.metastasis and TNM stage(P＜0.05).The expression of MMP-9 was also associated with the degree of differentiation of gastric cancer(P＜0.05).There was a correlation between COX-2 and MMp-9 expressions in gastric cancer tissues(P＜0.05,C=0.359).The expression of Ki67 in tissues with positive expressions of COX-2 and MMp-9 was higher than that in negative ones(P＜0.O 1).Conclusion The COX-2,MMP-9 and Ki67 play important roles in metastasis and infiltration,which promote occurrence and development of gastric cancer.

Objective To explore the technique of auxiliary partial orthotopic liver transplantation model in C57BL/6 mice.Method The donor portal vein and hepatic vein were anastomosed with Cuff and suture techniques respectively.The donor bile duct was implanted into recipient duodenum.Result The operation time of harvesting donor's liver and anhepatic phase and recipient was (30 ± 3),(6-± 1) and (58 ± 5) min respectively.The model success rate was 96％,and the 4-week survival rate was 88％.Conclusion The animal model was stable with high success-rate and can be used for the study of auxiliary partial orthotopic liver transplantation.

Southern blotting with a labeled and linearized pUC 19 DNA containing a specific fragment of 0. 24 kb DNA of Plasmodium vivax asexual blood stages (kindly offered by Dr. C. Kidson )was used for further identification of blood samples showing positive reaction by dot-blot hybridization. The results showed that those with positive reaction from patients with P. vivax, with P. falciparum or with fever but with negative microscopic findings were also positive by Southern blotting. It was confirmed that some of those positive with P. falciparum were likely to infect P. vivax at the same time. So did a part of those with fever but negative in the blood films (Figs. 1,2).

0.05) .The total survival rate of N group and H I group was significantly higher than H II group(P0.05) .The abdominal stinking leak was more severe and death rate was higher in N group and H I group than H II group.Conclusion:Dry heat environment in the desert could obviously impair the total survival rate of pigs.

Objective To study the effect of core stability training on motor function in patients with hemiplegia after stroke. MethodsSixty-eight patients with hemiplegia after stroke were randomly divided into a treatment group (34 cases) and a control group (34 cases).Both groups were given regular rehabilitation training.The patients in the treatment group also were taught core stability training.The trunk control test (TCT),Berg's balance scale (BBS),the modified Barthel index (MBI),functional ambulation categories (FACs) and the Fugl-Meyer assessment scale (FMA) were used to assess motor function before and after treatment. ResultsThere were significant differences between the two groups in average TCT scores,BBS scores,FACs,M BI scores and FMA scores after treatment.The gains in the treatment group were significantly superior to those in the control group.The patients'trunk control was positively correlated with the BBS,MBI,FAC and FMA results.Conclusion Core stability training can improve the motor function of patients with hemiplegia after stroke.

Objective To study their chemical components of xianglingcao(Crotalaria ferruginea) and identify their chemical structures.Methods The compounds were isolated by chromatography and their structures were identified by spectral analysis and compared with the published data.Results Ten compounds were isolated and identified as vomifolilol(Ⅰ),genistein(Ⅱ),p-hydroxybenzoic acid(Ⅲ),5,7-dihydroxy-4-methoxy-flavone-7-O-?-D-glycopyranoside(Ⅳ),octadecoic acid(Ⅴ),octacosanol(Ⅵ),?-stitosterol(Ⅶ),?-daucosterol(Ⅷ),stigmasterol(Ⅸ),and ?5,22 stigmasterol-3-O-?-D-glycopyranoside(Ⅹ).Conclusion The ten compounds are obtained from xianglingcao for the first time.