1.Analysis and Evaluation on Methodology of Serum Pharmacology Research in Traditional Chinese Medicine and its Complex Prescription
International Journal of Traditional Chinese Medicine 2009;31(2):171-173
The study of traditional Chinese medicine and its complex prescription gradually adopted the methods of serum pharmacology, which has become a new researching thought and a gained more attention. In this article, the background of relevant researches, methodological discussions, analysis of the advantages and problems of serum pharmacology were reviewed. The serum pharmacology, for its unique value, will certainly has a brilliant prospect in its application to the research of traditional Chinese medicine and its complex prescription.
2.Caveolin-1 with tumour
International Journal of Surgery 2013;(6):398-401
Caveolin are a family that proteins are highly conserved and located at the mammalian plasma membrane.They include four isoforms:Cav-1,Cav-2,Cav-3 and Cav-4.Caveolin proteins are implicated in endocytosis,lipid metabolism and cellular signaling.There is recent evidence that Cav-1 expression levels in tumour have been associated with tumour growth and metastasis,stromal car-1 expression downregulated have been also associated with mutli-tumor metastasis.
3.Toxic effect of etomidate on porcine adrenal cortical cells and influence of etomidate preconditioning
Dongnan HOU ; Xiaoping ZHANG ; Junyu XIONG
Chinese Journal of Anesthesiology 2010;30(9):1088-1090
Objective To investigate the effect of etomidate on porcine adrenal cortical cells and the influence of preconditioning with small dose etomidate. Methods Porcine adrenal cortical cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 15% fetal bovine serum at 37℃ in 5% CO2 incubator for 24 h. The concentration was 2 × 106/ml. The experiment was performed in 2 parts. In part Ⅰ the cells were exposed to 50, 100, 200, 300, 400, 500 and 1000 mol/L etomidate respectively and incubated for 6, 12and 24 h, while in control group, the cells were exposed to 0.5% dimethylsulfoxide (DMSO). Cell viability was measured using CCK-8 assay and apoptosis by flow cytometry, and the 50% inhibitory concentration (IC50) of etomidate was calculated at 24 h of incubation. In part Ⅱ the cells were exposed to 0.6 μmol/L etomidate for 1 h and were allowed to recover for 4 h after etomidate washout, then etomidate 325 μmol/L was added and the cells were incubated for 24 h. Cell viability and apoptosis were assessed and the IC50 of etomidate was calculated as in part Ⅰ .Results Etomidate inhibited viability of porcine adrenal cortical cells and induced apoptosis in a dose- and time-dependent manner. The IC50 of etomidate at 24 h of incubation was 325 μmol/L. Preconditioning with0.6 μmol/L etomidate for 1 h attenuated the apoptosis induced by etomidate 325 μmol/L. Conclusion Etomidate can inhibit cell viability and induce apoptosis in a dose- and time-dependent manner. Preconditioning with small dose etomidate has protective effect.
4.The effects of 54Ala/Thr polymorphism in intestinal fatty acid binding protein (IFABP) on serum lipids in middle aged and senile people
Zhenhui WANG ; Xiaotong CHANG ; Xiaoping HOU
Medical Journal of Chinese People's Liberation Army 1982;0(01):-
0.05). The LDL-C and apoB concentrations in fasting serum in men with 54T allele were significantly higher than those with 54A allele (2.38?0.63 vs 2.21?0.57mmol/L, P
5.Determination of four bufadienoiides in human liver by HPLC
Zhaohong WANG ; Xiaoping HOU ; Yi HE
Chinese Journal of Forensic Medicine 2002;0(06):-
Objective To establish a method used for quantitative determination of bufadienolides in human liver tissues by HPLC. Methods using solid phase extraction of human liver with Oasis (HLB car-tridge coupled with reserved phase HPLC and diode array detection. Results Recoveries obtained from spiked liver for the bufadienolides were better than 70% . The linearity was studied up to 1200ng/g and the detection limits of the method was 0.4ng for cinobufotalin and bufalin, 0.5ng for cinobufagin and resibufo-genin. Conclusion This method is fast and accurate. It is useful for forensic medicine detection.
6.Change of mast cell in gastric mucosa of patients with gastric hypersensitivity
Liangru ZHU ; Xiaoping XIE ; Xiaohua HOU ;
Chinese Journal of Digestion 2001;0(02):-
Objective To investigate whether gastric hypersensitivity exists in functional dyspepsia patients, and to observe the number of mast cells in proximal gastric mucosa in gastric hypersensitivity patients and the change of mast cell degranulation after stimulation so as to evaluate the role of mast cells in gastric hypersensitivity. Methods Gastric sensation threshold was determined using barostat in 40 functional dyspepsia (FD) patients and 15 controls. Twenty three patients were divided into two groups on the basis of perception threshold: hypersensitivity group (FD H) and normal sensation group (FD N). Mast cells were stained by immunohistochemistry method, mast cell number was calculated by microscopy and the average optical density valves( A ) of mast cell were obtained by microspectrophotometry (MSP). The ultrastructure of mast cell was observed under electromicroscopy. Results Gastric sensation threshold (i.e. perception, discomfort and pain threshold) was significantly lower in FD patients than in controls ( P
7.Expressions of cyclooxygenase-2,matrix metalloproteinases-9 and Ki67 in tissues of gastric cancer and their clinical significance
Long BAI ; Peizhen HOU ; Xianwen HOU ; Juan ZHANG ; Xiaoping ZOU
Chinese Journal of Digestion 2009;29(3):183-185
Objective To investigate the association of expressions of cyclooxygenase-2(COX-2),matrix metanoproteinases-9(MMp-9)and Ki67 with occurrence,infiltration and metastasis of gastric cancer.Methods Fifty-eight surgically obtained and pathologically performed cancerous tissues were collected between Jan.2003 and Dec.2005.Among them,37 were males and 21 were females with mean age of 58.2 years(ranged from 31 to 76 years).Fifty-eight paracancerous tissues(5-6 cm apart form the cancer)were taken as controls.The expressions of COX-2,MMP-9 and Ki67 in cancerous or paracancerous tissues were examined by immunohistochemistry.Results The expressions of COX-2 and MMP-9 in cancerous tissues(82.76%and 68.9%,respectively)were higher than those in controls(37.93%and 24.14%,respectively,P<0.01).The expressions of COX-2 and MMP-9 were not associated with sex,age,location and size of gastric cancer(P>0.05),but were correlated with the depth of lymph node infiltration.metastasis and TNM stage(P<0.05).The expression of MMP-9 was also associated with the degree of differentiation of gastric cancer(P<0.05).There was a correlation between COX-2 and MMp-9 expressions in gastric cancer tissues(P<0.05,C=0.359).The expression of Ki67 in tissues with positive expressions of COX-2 and MMp-9 was higher than that in negative ones(P<0.O 1).Conclusion The COX-2,MMP-9 and Ki67 play important roles in metastasis and infiltration,which promote occurrence and development of gastric cancer.
8.Relationship between villus BaP-DNA adducts level and blighted ovum in early pregnancy
Haiyan HOU ; Zhenhua YANG ; Xiaoping ZOU ; Yaqiong CHEN
Chinese Journal of Perinatal Medicine 2011;14(8):459-462
Objective To investigate relations between villus Benzo (a)pyrene (BaP)-DNA adducts and blighted ovum in early pregnancy, and to explore possible environmental factors influencing embryo development. Methods One hundred and two pregnant women with blighted ovum were selected into this study as research group; and 102 normal pregnant women were taken as control group; the age, gravidity, parity and gestational weeks of the two groups were matched. After artificial abortion, villi of the patients were collected and washed by normal saline. Then, the tissue was homogenated and genome DNA was extracted to detect quantity of the tissue. BaP-DNA adducts levels were examined by high-performance liquid chromatography-fluorescence method (HPLC). The personal information of pregnant women was collected by questionnaire. Logistic regression model was used to investigate the association between BaP-DNA adducts and blighted ovum. Results BaP-DNA adducts level in villi of research group [(8. 9±8. 2) adducts/108 nucleotides] was significantly higher than that of control group [(2. 0±1. 4) adducts/108 nucleotides], P<0.05. The higher the BaP-DNA adducts in villi, the higher risk the blighted ovum; when the BaP-DNA adducts level increased to 6.06 adducts/108 nucleotides, the risk of blighted ovum might increase 59.39 times (95% CI:15.50-227.55). Maternal education level was a protective factor (OR=-0.21, 95%CI:-0. 19--0.03) after controlling potential confounders. Conclusions High level of villi BaP-DNA adducts might increase the risk of blighted ovum in early pregnancy, and could have an adverse effect on embryo development.
9.NK and NKT cells in acute pancreatitis
Zhining LIU ; Xiaoping GENG ; Shengyun WAN ; Hui HOU ; Zongfan YU
Chinese Journal of General Surgery 2016;31(12):1031-1033
Objective To investigate natural killer(NK) and NKT cells in acute pancreatitis(AP).Methods Changes of NK and NKT cells in peripheral blood of 86 AP cases were detected using muhiparameter flow cytometry.Results Compared with control group,the NKT cells decreased in AP patients (t =5.23,P =0.00),but NK cells didn't (t =-1.15,P =0.25).NKT cells in severe SAP and mnoderate MAP were lower than that in the control group (t =-3.92,P =0.00;t =4.84,P =0.00).There was no statistically significant difference of NK cells between MAP and the controls (t =-0.54,P =0.59),but NK cells in SAP group was obviously higher than that in control group (t =3.12,P =0.00).After one week treatment,NK cells significantly decreased (t =8.43,P =0.00).NKT cells were higher than control group (t =-4.44,P =0.00).Dynamic monitoring in AP patients found continuous declination in NK cells,and NKT cells experienced an increase before a falling.Conclusion Monitoring of NK and NKT cells can be used as an important index for the severity and response to treatment in acute pancreatitis.
10.Efficacy of subcutaneous administration of gonadotropin-releasing hormone agonist on idiopathic central precocious puberty.
Yan, LIANG ; Hong, WEI ; Jianling, ZHANG ; Ling, HOU ; Xiaoping, LUO
Journal of Huazhong University of Science and Technology (Medical Sciences) 2006;26(5):558-61
In order to assess the feasibility of subcutaneous administration of Triptorelin with 6-week intervals for the suppression of pituitary-gonadal axis and changes of clinical signs in girls with idiopathic central precocious puberty (ICPP), 46 girls with ICPP were treated with GnRHa. Triptorelin (Decapeptyl, 3.75 mg) was administered subcutaneously (SC) at 6-weeks intervals or intramuscularly (IM) at 4-weeks intervals randomly for more than 12 months consecutively. During GnRHa therapy, clinical parameters and laboratory data, including height, weight, pubertal stage, bone age, uterine volume and ovarian size, serum levels of luteinizing hormone (LH), follicle stimulating hormone (FSH) and estradiol (E2), were monitored and analyzed. It was found that both treatment regimes led to regression of precocious puberty and reversal of secondary sexual characteristics. Breast developments regressed. Uterine volume was decreased after treatment, but there was no statistically significant difference. Mean ovarian volume did not change significantly during treatment. The height velocity was decreased significantly from 6.3+/-1.4 cm/year to 5.8+/-1.2 cm/year in group SC and 6.7+/-1.3 cm/year to 5.4+/-1.0 cm/year in group IM, respectively. The rate of bone maturation was reduced significantly during treatment. The ratio of deltaBA/deltaCA was 1.2+/-0.2 or 1.3+/-0.3 at the onset of therapy and decreased significantly after the treatment to 0.7+/-0.2 or 0.9+/-0.1, respectively. The predicted adult height was increased significantly and progressively during therapy. The levels of serum LH, FSH and E2 returned to the prepubertal condition. No significant side effects of therapy were noted. The most common side effect during SC treatment was that a non-irritating, 1 cm in diameter mass was palpated at the site of subcutaneous injection in the abdominal wall of patients, which disappeared after 6-12 weeks. Two girls had minimal withdrawal vaginal bleeding episodes after the first injection. It was concluded that both IM and SC triptorelin administrations were clinically effective. They induce profound suppression of hypothalamic-pituitary-gonadal axis while stabilizing height velocity, slowing bone maturation and increasing predicted adult height. These results suggest that subcutaneous injection of triptorelin in 6-weeks intervals at a dosage of 3.75 mg be a safe and acceptable regimen for ICPP