AIM:To observe the expression of phosphorylated cell cycle related proteins cyclin-dependent kinase(CDK) and the retinoblastoma protein(Rb) in the developing and aging rat hippocampus.METHODS:The immunofluorescent staining for anti-phospho-CDK2,anti-phospho-CDC2,anti-phospho-Rb and anti-NeuN antibodies was used to determine their expressions and distributions in Wistar rat hippocampus at different ages.The expressions of phosphorylated cell cycle related proteins were also assessed by Western blotting analysis.RESULTS:(1) The number of neurons of CA1 area in hippocampus decreased during the period from 1 day to 15 months.(2) The positive expressions of phospho-CDK2 and phospho-Rb increased with aging.(3) No apparent change in the expression of phospho-CDC2 in the hippocampus of all the five groups was observed.CONCLUSION:The expressions of phospho-CDK2 and phospho-Rb suggest that differentiated neuron can re-enter the cell cycle and more neurons enter the cell cycle in the aged rat brain.Re-entering the cell cycle of differential neurons probably results in apoptosis.

Objective To investigate the relationship between air pollution and the acute respiratory symptoms in children in Taiyuan,China.Methods A case-crossover design was used to study the association between air pollution and the acute respiratory symptoms of 254 children aged 7-11 in a school in 2005.Results The acute respiratory symptoms(cough,nose running,stuffy nose and angina)increased as the air concentration of PM10 increased.Conclusion There seems to be a positive association between air pollution of PM10 and the acute respiratory symptoms in children in Taiyuan.

Objective To explore the relationship between insulin resistance (IR) and the activity of plasmingin activator inhibitor-1 (PAI-1) in acute and recovery phases of the patients with arteriosclerotic cerebral infarction (ACI).Methods The levels of fasting serum glucose and free serum insulin were examined, while the activity of tissue-type plasmingin activator (t-PA) and PAI-1 were determined in 91 ACI patients and 40 healthy controls. Insulin sensitivity index (ISI) was estimated by the negative natural logarithm of the verse of fasting serum glucose and insulin product.Results In the acute and recovery phases of the patients with ACI,compared with healthy control group the levels of fasting serum glucose, insulin and activity of PAI-1 were much higher ( P

BACKGROUND: The hot environmental condition of the isolated severe acute respiratory syndrome (SARS) ward makes the protein transformation of the medical personnel accelerated. The dietary protein should make up the special consumption and improve the immunity in addition to guaranteeing the normal metabolism for the purpose to promote the organic endurance and resist the communicable disease.OBJECTIVE: To study the scientific mode of quality and quantity of protein supply for the medical staff working in the SARS ward.DESIGN: A cross section analysis.SETTING: Trophological Department, General Hospital of Chinese People's Armed Police Forces.PARTICIPANTS: The 64 healthy doctors and nurses, 22 males and 42females, aged 29 averagely, worked in the SARS ward from May 1st to 20th 2003, were the volunteers to join the observation.METHODS: The weighing method was applied to measure the quantity of various food supplied by recipe and actually taken and the nutrition software was used to analyze the quantity of protein and amino acid contained in the food. The quantity of protein supply (taking male as standard) calculated according to the heavy consumption, 1.5 g/kg.d, in the hot environment was compared with the estimated consumption, male (108.00±12.63) g and female (85.21±9.55) g, and with the actual intake analyzed by computer. The corrected scoring of digestibility was employed to evaluate the reasonability of intake of various essential amino acids. The quantity of essential amino acids contained in every gram of protein of recipe/the amino acids contained in Reference Mode of Chinese Food × 93% being higher than 1.0 was said to be the quality protein meeting the need of human body.MAIN OUTCOME MEASURES:① The quantity and composition of protein supplied by the recipe. ② The quantity and composition of protein actually taken by the working staff.RESULTS: All of the 64 medical working staff entered the result analysis.① The protein supply of recipe (daily average) could totally meet the need of estimated consumption of male and female staff [(157.36±10.43) g,(108.00±12.63) g, (85.21±9.55) g, t=9.344, 18.278, P ＜ 0.01] and the need of actual intake [(157.70±58.14) g, (111.02±31.77) g, t= -0.015, 3.795, P＞ 0.05, P ＜ 0.01]. ② The corrected scores of 9 amino acids of digestibility, (1.9-3.1), were all higher than 1.0 and the percentage of amino acids was in accordance with the food mode of Chinese people.CONCLUSION: The protein quantity and quality of recipe can meet the special need of medical staff working in the hot environment with the heavy physical consumption in the SARS ward and can produce the best biological effect, being scientific.

Child ; China ; Endocrinology ; history ; History, 20th Century ; History, 21st Century ; Humans ; Pediatrics ; history

Objective To investigate the correlation among mammographic features, and estrogen /progesterone receptor(ER,PR) of the breast cancer.Methods The mammographic features of 60 cases of breast cancer confirmed by surgery and histology were analyzed retrospectively. The specimens of mastectomy of the breast cancer were stained with immunohistochemistry,the expression of estrogen receptor (ER) and progesterone receptor (PR) were measured .The relationship between the immunohistochemical pathologic results and mammographic features were analyzed. Results Among the 60 cases of breast cancers, X-ray types mass of breast cancer were correlated with the expression of PR (P

Objective To systematically analyze and assess the risk of venous thromboembolism in the patients with rheuma‐toid arthritis(RA) .Methods The related literatures on the venous thromboembolism occurrence in the patients with RA published at home and abroad were performed the electronic retrieval .The obtained data were analyzed by adopting the RevMan5 .1 software . The data unable to merge were analyzed by adopting the descriptive analysis method .Results A total of 10 related papers were in‐cluded .The 3 indicators of venous thromboembolism occurrence rate ,deep vein thrombosis occurrence rate and pulmonary thrombo‐embolism occurrence rate were performed the meta analysis .The RR and 95% CI of the occurrence rates of venous thromboembo‐lism ,deep vein thrombosis and pulmonary thromboembolism were 2 .00(1 .71 ,2 .33) ,2 .31(1 .84 ,2 .90) ,2 .25(2 .23 ,2 .28) respec‐tively .Conclusion The risk degree of venous thromboembolism occurrence in the RA patients is higher than that in the non‐RA pa‐tients .

To develop a portable oxygen moisture bottle. The bottle adopted a double-tightened and separate structure, whose shell was made of transparent materials. The inner liner was made of PTFE hydrophobic mem-brane filter, which applied a 3-layer structure to inlet oxygen into the shell through the air membrane filter at all directions. The bottle had no requirements for site and high reliability, and the desired use for continuous oxygen inhalation was realized. The bottle has a simple structure, low cost, easy maintenance and high portability, and gains ad-vantages over the traditional one.

Aim To investigate the effect of melatonin (MT) and pentoxifylline (PTX) on hepatic ischemia reperfusion (I/R) in rat. Methods 128 rats were randomly divided into four groups: control group, MT treated group, PTX treated group and PTX+MT group (combined group). Those operation procedures including occlusion the inflow to the liver 35 minutes, hepatectomy and reperfusion, were underwent by the animals. The following items were investigated: liver function, endothelin 1 (ET 1), malondialdehyde (MDA), superoxide dismutase (SOD), and one week survival. Results The following changes were detected after the liver ischemia: ①Liver function: the increased scopes of ALT and LDH were significantly lower in treatment groups, especially in the combined group than that in control one; ②ET 1: the levers of ET 1 were significantly increased in every group, especially the control group; ③MT function: the increased scopes of MDA and SOD were significantly lower in MT groups (including MT treated and combined groups) than that in others; ④PTX function: the increased scopes of TNF ? were significantly lower in PTX groups (including PTX treated and combined groups) with a higher rate of survival than that in others. Conclusion ①MT could reverse the oxidative damage, but cannot affect the rate of survival; ②PTX can decrease the secretion of cytokine and the one week survival; ③Based on the synergistic effects, the application of MT+PTX may have beneficial effects against the I/R injury to increase the survival.