Objective To analyze the incidence,mortality and survival rates of pancreatic cancer in Pudong New Area of Shanghai from 2002 to 2010.Methods The residents in Pudong New Area of Shanghai were recruited in this study during the period 2002 ～ 2010,the incidence,mortality were calculated according to different age groups and genders.The standardized morbidity and mortality of pancreatic cancer were calculated by world standard population.Logarithmic linear regression was used to calculate the annual percentage change (APC) of incidence and mortality.The 1 ～ 5 year survival of pancreatic cancer patients was analyzed by Kaplan-Meier method and COX regression analysis,and the survival of patients with different TNM staging,with or without operation was determined.Results Among 3089 newly occurred pancreatic cancer cases during 2002 ～ 2010,1707 and 1382 cases were males and females,respectively,with an average age of (69 ± 12) and (73 ± 12) years old,the crude incidence for both genders was 13.32/100 000,and it was 14.71/100 000 for males,which was higher than that in females (11.93/100 000).The ratio of male and female for incidence of age standardize was 1.57:1.There were 2963 death in total,including 1627 males and 1336 females,with a crude mortality rate of 12.78/100 000.The crude mortality rate for males was 14.02/100 000,which was higher than that in females (11.53/100 000).The ratio of male and female ASR for mortality was 1.55:1.Both incidence and mortality significantly increased for males aged over 35 and females aged over 40.The peak of morbidity and mortality appeared in male over 80 years old,and in female over 85 years old.The 1 ～ 5 year survival rates of pancreatic cancer patients were 16.59％,7.31％,5.23％,4.33％ and 3.87％,respectively.The differences in 1 ～5 year survival rates between surgical and non-surgical management groups were statistically significant (P ＜ 0.05).The median survival time of TNM 0 ～ Ⅱ,Ⅲ and Ⅳ staging was (250.00 ± 33.37),(224.00 ± 15.82),(86.00 ± 4.52) d.There was a statistically significant difference among the survival of TNM-Ⅳ and TNM 0 ～ Ⅰ,TNM Ⅲ (P ＜ 0.001).Conclusions The incidence and mortality of pancreatic cancer in males are higher than those in females in Pudong New Area of Shanghai.The survival is associated with TNM staging at diagnosis and whether surgical operation is performed.

Objective:To compare the efficacy between percutaneous vertebroplasty (PVP) with unilateral double-target puncture approach plus side opening propeller and traditional PVP in the treatment of Kümmell disease.Methods:A retrospective cohort study was conducted to analyze the clinical data of 40 patients with Kümmell disease admitted to Zhengzhou Orthopedic Hospital from January 2020 to June 2022, including 11 males and 29 females; aged 61-95 years [(74.3±9.5)years]. Twenty patients received the PVP with unilateral double-target puncture approach plus side opening propeller (observation group), and 20 patients were treated with traditional PVP (control group). The operation time, volume of cement injection and distribution levels of bone cement were compared between the two groups, as well as the visual analogue score (VAS), Oswestry dysfunction index (ODI), Cobb angle, anterior height of the vertebral body before operation and at 1 day and 6 months after operation. The incidence of complications was also compared between the two groups.Results:All patients were followed up for 6-12 months [(9.1±2.8)months]. The operation time was (40.3±4.5)minutes in the observation group and (38.6±3.5)minutes in the control group ( P>0.05). The volume of cement injection was (6.5±1.3)ml in the observation group, significantly more than that in the control group [(5.4±1.0)ml] ( P<0.01). The distribution levels of bone cement in the observation group (0 patient in Grade 1, 3 in Grade 2, and 17 in Grade 3) were better than those in the control group (1 patient in Grade 1, 15 in Grade 2, and 4 in Grade 3) ( P<0.01). There were no significant differences in the VAS, ODI, Cobb angle or anterior height of the vertebral body between the two groups before operation (all P>0.05). One day after operation, the VAS in the observation group and control group was (1.6±0.7)points and (2.5±0.8)points, with the ODI of 16.1±3.3 and 21.3±4.1, Cobb angle of (18.4±2.9)° and (21.4±2.0)° and anterior height of the vertebral body of (23.7±1.8)mm and (20.1±1.7)mm. Six months after operation, the VAS in the observation group and control group was (1.3±0.5)points and (2.3±0.9)points, with the ODI of 15.0±3.8 and 20.8±3.9, Cobb angle of (19.3±2.9)° and (22.4±1.7)°, and anterior height of the vertebral body of (23.4±1.8)mm and (19.8±1.6)mm. The VAS, ODI, Cobb angle and anterior height of the vertebral body in the observation group were better than those in the control group at 1 day and 6 months after operation (all P<0.01). The VAS, ODI, Cobb angle and anterior height of the vertebral body were significantly improved in the two groups at 1 day and 6 months after operation compared with those before operation (all P<0.01), but there were no significant differences between 1 day and 6 months after operation (all P>0.05). The incidence of complications was not significantly different between the two groups ( P>0.05). Conclusion:The PVP with unilateral double-target puncture approach plus lateral opening propeller not only allows for adequate and evenly distributed injection of bone cement, but also effectively reduces the pain, improves the quality of life, corrects the kyphosis and restores the height of the vertebral body in treating Kümmell disease compared with the traditional PVP.

Atherosclerosis(AS)is a chronic inflammatory disease of large and medium-sized arteries that leads to ischemic heart disease,stroke,and peripheral vascular disease.Despite the current treatments,mortality and disability still remain high.Sonodynamic therapy(SDT),a non-invasive and localized methodology,has been developed as a promising new treatment for inhibiting atherosclerotic progression and sta-bilizing plaques.Promising progress has been made through cell and animal assays,as well as clinical trials.For example,the effect of SDT on apoptosis and autophagy of cells in AS,especially macrophages,and the concept of non-lethal SDT has also been proposed.In this review,we summarize the ultrasonic parameters and known sonosensitizers utilized in SDT for AS;we elaborate on SDTs therapeutic effects and mechanisms in terms of macrophages,T lymphocytes,neovascularization,smooth muscle cells,lipid,extracellular matrix and efferocytosis within plaques;additionally,we discuss the safety of SDT.A comprehensive summary of the confirmed effects of SDT on AS is conducted to establish a framework for future researchers.

Background and objective Different histologies of lung cancer vary in occurrence and prognosis. hTis study aims to analyze the incidence and occurrence trend of lung cancer and investigate the survival rate and its inlfuential fac-tors among lung cancer patients with different histologies. Methods Permanent residents were recruited between 2002 and 2009 in Pudong New Area (former Nanhui Area and former Pudong Area), Shanghai, China. Annual percent changes were estimated by a linear regression of the logarithm on the incidence rates for eight years. Survival rates were calculated and com-pared by using life-table analysis and Log-rank test, respectively. Results hTe standardized incidence rates of lung cancer were 52.28 and 18.86 per 100,000 in males and females, respectively. hTe median survival time was 410.72 days for speciifc classiifed lung cancer. hTe incidence rates of adenocarcinoma ranked the highest and showed an upward tendency (P<0.05). Patients with small cell lung cancer showed the worst survival condition. hTe survival condition in males with squamous cell lung can-cer living in former Nanhui Area was better compared with those living in former Pudong Area. Conclusion Lung cancers with different histologies demonstrated different occurrence trends and survival rates. Gender, age, and living area inlfuence the sur-vival rates of lung cancer with different histologies.

Objective: To investigate the function and mechanism of golgi protein 73(GP73) neutralizing antibody in Methionine-choline deficient(MCD) diet-induced non-alcoholic fatty liver disease(NAFLD). Methods : Total cholesterol(TC),aspartate aminotransferase(AST),triglyceride(TG) and serum alanine aminotransferase(ALT) levels were detected after MCD diet and GP73 neutralizing antibody intervention. Lipid deposition in liver tissue was observed by oil red O staining; qRT-PCR was used to detect the gene expression ofACC1,HMGR,TIMP1andTGF-βin liver tissue; Western blotting was used to detect α-SMA and SREBP1 protein expression levels in liver tissues. Results : GP73 neutralizing antibody could reduce the accumulation of serum ALT and AST(P<0.001) induced by MCD diet, and increase the levels of serum TG and TC(P<0.001);The gene expression levels ofHGMR(P<0.001),TGF-β(P<0.05),ACC1(P<0.01) and the protein expression levels of α-SMA(P<0.05) and SREBP1(P<0.05) in liver tissue significantly decreased, and the lipid deposition was also improved. Conclusion GP73 neutralizing antibody inhibits the formation of MCD-induced NAFLD by slowing down the progression of liver fibrosis and reducing the deposition of liver lipids.

[Objective] To perform genetic analysis on samples with weak agglutination and mixed agglutination of ABO blood group antigens in neonates, and to investigate the molecular biological characteristics of ABO subtypes in neonates. [Methods] Serological identification of ABO blood group was performed by tube method and microcolumn gel method. The ABO exons 2-7 were amplified by PCR, and the amplified products were sequenced by Sanger sequencing method to determine the genotype. [Results] Among the ABO blood group serological results of 14 neonates, 8 cases showed weakened A antigen, and 6 cases showed weakened B antigen. Seven samples were identified with ABO subtype alleles, with genotypes as A102/B101+c.538C>T, Aw26/B102, A205/O02, A205/B101(2 cases), Aw26/O02, B(A)06/O01, B101/O01(3 cases), A102/O01(2 cases), A102/B101 and B101/O02. Additionally, three other family members were also found to carry B(A)06 allele in a pedigree investigation. [Conclusion] For samples showing weakened antigens in ABO blood type identification of neonates, it is necessary to consider the possibility of ABO subtype in addition to age factors, and genetic testing can be used to prevent missed detection of ABO subtypes in neonates.