1.Effects of recombinant human acidic fibroblast growth factor on healing of dermal chronic ulcers in diabetic rats
Qing LIU ; Weiying GUO ; Kehui LIU ; Xiaona XIE ; Liying WANG
Journal of Jilin University(Medicine Edition) 2006;0(01):-
Objective To study the healing effect of recombinant human acidic fibroblast growth factor(rhaFGF) expressed in E.coli on dermal chronic ulcers in diabetic rats.Methods Ten male Wistar rats were used to set up diabetic dermal chronic ulcers models.The wounds were sprinkled with rhaFGF and physiological saline,respectively.The wound area,wound cavity volume and healing time were recorded,granulation tissue growth and epithelization in wound were observed,and wound healing status was evaluated.Results Compared with control group,the wound area and wound volume at different day in rhaFGF group were significantly diminuted(P
2.Effect of Neuromuscular Facilitation Technique and Swallowing Training Combined with Real-time Electrical Stimulation on Patients with Dysphagia after Stroke
Xuan WANG ; Yongmei ZENG ; Hong TIAN ; Min LI ; Xiaona XIE
Chinese Journal of Rehabilitation Theory and Practice 2013;19(7):651-654
Objective To observe the effect of neuromuscular facilitation technique and swallowing training combined with real-time electrical stimulation on dysphagia after stroke. Methods 50 patients with dysphagia were divided into treatment group (n=25) and control group (n=25).The treatment group accepted neuromuscular facilitation technique and VOCASTIM.The control group accepted routine vocal training and low frequency pulse electrical stimulation. They were assessed with the deglutition function classficaition and water drinking test. Results There was significant improvement in the both groups 2 weeks and 4 weeks after treatment (P<0.05), and it improved more in the treatment group than in the control group (P<0.05). Conclusion Neuromuscular facilitation technique and swallowing training combined with real-time electrical stimulation can promote the recovery of function of patients with dysphagia after stroke.
3.Correlation between sleep quality and anxiety in CKD patients
Qiong XIE ; Rongrong WANG ; Yongmei LU ; Xiaona XU ; Chaohua PENG ; Menghui CHENG
Modern Clinical Nursing 2017;16(1):1-4
Objective To study the correlation between sleep quality and anxiety in patients with chronic kidney diseases (CKD).Method Toally 118 patients with CKD were investigated with Pittsburgh sleep quality index (PSQI) and self-rating anxiety scale (SAS).Results About 75.42% (89/118) patients had poor quality of sleep.The scores by PSQI and the dimensions were higher than those of the norms (all P<0.05),but the total scores by PSQI and the factors of sleep quality,time of falling sleep,duration of sleep,use of hypnosis drugs were all significantly lower than those of the insomnia group (all P<0.01).35.59% (42/118) of the patients had anxiety and the score of SAS was significantly higher than that of the national norm (P<0.01).The total scores of PSQI,sleep quality,sleep time,sleeping time,sleep disorder,daytime function were positively related to the score of SAS (P<0.01).Conclusions The quality of sleep is poor in the CKD patients and there is a positive correlation between the quality of sleep and the anxiety.It is necessary to strengthen the knowledge-related health education and to provide emotional support and individualized nursing services for CKD patients so as to relieve their anxiety and improve their sleep quality.
4.Punica granatum seed oil inhibits malignant behavior of breast cancer cells
Guoqiang FU ; Lu LIU ; Lei ZHANG ; Yuan GAO ; Xiaona XU ; Feng XIE ; Feng WANG
Military Medical Sciences 2015;(6):438-442
Objective To study the effect of Punica granatum( pomegranate) see d oil( PSO) on proliferation and apop-tosis behaviors of breast cancer cells.Methods Fatty acid composition was detected by gas chromatography,breast cancer cells, MCF-7 and MDA-MB-231 were treated with PSO, cell proliferation was observed by MMT, cell apoptosis was analyzed by flow cytometry,and expression levels of proliferation and apoptosis-related proteins were detected by Western blot.Results Punicic acid (PA) was the major fatty acid in PSO(74.41%).PSO could inhibit the proliferation while in-ducing apoptosis in both cell lines in a dose-and time-dependent manner, significantly decrease the expression level of Cox-2 and Bcl-2, increase the expression level of Bax and caspase-3 (cleaved),remarkably upregulate the expression of P53 in MCF-7, and downregulate p53 expression in MDA-MB-231.Conclusion PA may be one of the functional ingredients of PSO which can inhibit proliferation and induce apoptosis in breast cancer cells.These effects are probably mediated by regu-lating the expression of Cox-2, Bcl-2, Bax, caspase-3 (cleaved) and p53.
5.Evidence summary of risk grading management of high-risk diabetic foot patients
Xiaona SUN ; Ziwei XIE ; Jinju WU ; Chun'e LIU
Chinese Journal of Modern Nursing 2024;30(30):4089-4095
Objective:To retrieve and evaluate the evidence on the risk grading management of high-risk diabetic foot patients, so as to provide reference for the management of diabetic foot.Methods:According to the "6S" pyramid model of evidence-based resources, evidence on risk grading management of high-risk diabetic foot patients was retrieved in Web of Science, UpToDate, Cochrane Library, PubMed, China National Knowledge Infrastructure, WanFang Data, VIP and other databases, guide websites, and professional association websites. The search period was from the establishment of the database to January 1, 2024.Results:A total of 11 articles were included, including 8 guidelines, one systematic review, one expert consensus, and one expert opinion. There were 24 pieces of best evidence about the risk grading management of high-risk diabetic foot patients, covering 8 aspects of risk assessment, risk grading, health records establishment, screening frequency, health education, foot management, exercise management and referral.Conclusions:The best evidence of risk grading management of high-risk diabetic foot patients summarized can provide reference for clinical nurses to carry out evidence-based practice.
6.A Comprehensive Assessment of the Racial and Ethnic Disparities in the Incidence of Gastric Cancer in the United States, 1992-2014
Qiang YAO ; Xiaona QI ; Wen CHENG ; Shao Hua XIE
Cancer Research and Treatment 2019;51(2):519-529
PURPOSE: This study aimed to evaluate the racial and ethnic disparities in the incidence of gastric cancer and their temporal trends in the United States. MATERIALS AND METHODS: Using data from 13 cancer registries in the Surveillance, Epidemiology, and End Results database, we assessed such disparities during 1992-2014 in the United States using a variety of disparity metrics. RESULTS: The age-standardized incidence rate of non-cardia gastric cancer was highest in Asian and Pacific Islanders, while the incidence of cardia gastric was highest in non-Hispanic whites in men and was similarly low in all groups in women. The incidence of non-cardia gastric cancer decreased in all groups over time, particularly in Asian and Pacific Islanders (on average by 3% per year). The incidence of cardia gastric remained relatively stable in virtually all racial/ethnic groups. The racial and ethnic disparities in gastric cancer incidence steadily decreased over time as measured on the absolute scale, which was mainly driven by the reduced disparities in non-cardia gastric cancer. The range difference in the incidence of gastric cancer decreased on average by 4.1% per year in men and by 2.6% per year in women from 1992 to 2014. The between group variance decreased by 5.6% per year in men and by 3.4% per year in women. The relative-scale disparity measures generally remained stable over time. CONCLUSION: This study demonstrates decreased racial and ethnic disparities in the incidence of gastric cancer over time in the United States, particularly as measured on the absolute scale.
Asian Continental Ancestry Group
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Cardia
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Epidemiology
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Female
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Humans
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Incidence
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Male
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Registries
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Stomach Neoplasms
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United States
7.Characteristics and influencing factors analysis of depressive symptoms among only-child-lost-persons
Xinmin ZHAO ; Cong XIE ; Xiaoling NI ; Xiaoju LI ; Sheng WU ; Xiaona WANG ; Zhen ZENG
Chongqing Medicine 2018;47(2):183-185
Objective To explore the characteristics and influencing factors of depressive symptoms among the only-child-lost persons.Methods A total of 841 only-child-lost persons and 674 controls conforming to entering group condition were selected.The difference of SDS score between the two groups and the demographic characteristics distribution differences of depression severity in only-child-lost persons were compared and the influencing factors were analyzed.Results The SDS score had statistical difference between the only-child-lost persons group and control group(P<0.05),and was correlated to the only-child-lost years and no physical disease;the proportion of mild to moderate depression in the only-child-lost persons of the low cultural level,somatic diseases and middle income level groups was significantly higher than that in the other groups;the only-child-lost persons with low income level,age≤60 years old and high educational level had a higher proportion of severe depression;the Logistic regression analysis showed that the educational level had a significant influence on the depression onset among the only-child-lost persons,the difference was statistically significant(P<0.05).Conclusion The characteristics of depressive symptoms among the only-child-lost persons are related to their demographic characteristics.
8.A dosimetric analysis of combined intracavitary/interstitial brachytherapy for locally advanced cervical cancer
Yongxia ZHANG ; Xiangkun YUAN ; Fumin SHI ; Jianwei HU ; Lei GAO ; Junjun MIAO ; Xiaona ZUO ; yuwei XIE
Chinese Journal of Radiological Medicine and Protection 2017;37(12):919-923
Objective To compare the dosimetric differences between intracavitary brachytherapy in combination with interstitial brachytherapy or not for locally advanced cervical cancer.Methods From May 2016 to March 2017,35 patients with locally advanced cervical cancer treated with combined external beams and intracavitary/interstitial brachytherapy were selected in this study.The prescription of intensity-modulated radiation therapy was:46.8-50.4 Gy/26-28 fractions,1.8 Gy/fraction.The prescription for combined intracavitary/interstitial brachytherapy was 7 Gy/fraction × 4,once per week.Each patient was first implanted with a three tube applicator for brachytherapy,and the CT images were acquired for treatment planning.The three tube applicator was removed before a uterine tube and needles were implanted,thereafter planning images were acquired again.Dose to the targets and organs at risk were evaluated respectively for the two groups.Results A total of 212 brachytherapy plans were developed,including 106 intracavitary and 106 endoluminal combined interstitial plans.The target dose in endoluminal combined interstitial brachytherapy was significantly higher than that of intracavitary treatment alone,where D90 of the high-risk clinical target volume (CTV) and moderate CTV were both significantly increased (t =-6.01,-2.73,P < 0.05).The D2 cm3 of the bladder,rectum and sigmoid colon were significantly reduced (t=3.07,4.52,2.91,P<0.05).Conclusions The application of the endoluminal combined interstitial brachytherapy for locally advanced cervical cancer can significantly increase the target dose,and reduce the dose to organs at risk such as the bladder,rectum and sigmoid colon.
9.Isolation and identification of rabbit-derived Lactobacillus reuteri and evaluation of its probiotic function
Yu CHENG ; Kun XIE ; Yanping JIANG ; Wen CUI ; Jiaxuan LI ; Xiaona WANG ; Xinyuan QIAO ; Lijie TANG ; Yijing LI ; Li WANG
Chinese Journal of Veterinary Science 2024;44(10):2136-2144,2293
Lactic acid bacteria(LAB)were isolated and cultivated from the intestinal contents of rabbits by MRS-CaCO3 solid medium.Identification was achieved through morphological observa-tion,Gram staining,physiological and biochemical characterisation,16S rDNA sequence analysis,and ERIC-PCR analysis.Strains displaying typical Lactobacilli characteristics were exanimated for their biological characteristics,resistance properties,adherence capacity in vitro,colonization abili-ty in vivo,and safety profile.In this study,a total of four strains of Lactobacillus reuteri were iso-lated from rabbits,all of which exhibited typical biological characteristics of LAB.These strains demonstrated inhibitory effects on common pathogenic bacteria in the gastrointestinal tract,with the primary inhibitory substance being bacteriocin.Furthermore,they showed sensitivity to chlor-amphenicol,rifampicin,and erythromycin,and displayed a degree of tolerance to gastrointestinal conditions and high temperature.These stains were capable of successful colonization in rabbits with a higher degree of safety.This study lays a foundation for the development of LAB prepara-tions for the prevention and treatment of rabbit intestinal diseases.
10.Comparison analysis of ultrasonographic characteristics of hepatic neuroendocrine neoplasm with different primary sites and pathological grades
Xiaona LIN ; Ming XU ; Jie CHEN ; Guangliang HUANG ; Luyao ZHOU ; Yu ZHANG ; Xiaoyan XIE ; Mingde LYU
Chinese Journal of Ultrasonography 2018;27(4):302-307
Objective To investigate the ultrasonography discrepancy of hepatic neuroendocrine neoplasm ( hNEN ) derived from different primary locations and pathological grades . Methods From August 2010 to April 2016 ,50 consecutive patients with 54 hNENs confirmed by pathology and imaging diagnosis underwent baseline ultrasound ( BUS ) and contrast-enhanced ultrasound ( CEUS ) in the First Affiliated Hospital of Sun Yat-sen University were enrolled in the retrospective study . On the basis of primary sites ,all hNENs were divided into three groups :pancreas ,gastrointestinal tract and other location groups . According to the pathological grading ,the hNENs were divided into hNET ( hepatic neuroendocrine tumor) group and hNEC ( hepatic neuroendocrine carcinoma) group . The imaging features of BUS and CEUS were retrospectively investigated and the ultrasonography discrepancy of hNEN derived from different primary focus and pathological grades were compared . Results ① Comparison of hNEN derived from different primary sites :hNEN derived from pancreas and gastrointestinal tract were smaller than hNEN derived from other sites[ ( 3 .8 ± 2 .6) cm vs ( 8 .7 ± 7 .0) cm , P = 0 .037 ;( 2 .9 ± 2 .1) cm vs ( 8 .7 ± 7 .0) cm , P = 0 .005] ,but the difference between the pancreas and gastrointestinal tract was not statistically significant ( P = 1 .0 ) . As for the pattern of CEUS enhancement ,hNEN derived from pancreas and gastrointestinal tract usually showed homogenous enhancement ( 13/19 ,15/23) while hNEN derived from other primary sites usually showed heterogeneous enhancement ( 10/13) ,and the difference was statistically significant ( P = 0 .025) . ② Comparison of hNET and hNEC :the distinction of enhancement level in the portal phase of contrast-enhanced ultrasound was statistically significant ( P = 0 .033) . All hNEC appeared hypo-enhancement ,while a small part of hNET ( 7/31 ) appeared iso-enhancement .More hNEC lesions showed heterogenous enhancemnt than hNET in the arterial phase ( 14/21 vs 9/31 , P = 0 .007) ,and there were less hNEC lesions presented tumor bleeding vessels than hNET ( 8/21 vs 22/31 , P = 0 .019 ) . Conclusions hNEN derived from pancreas and gastrointestinal tract are in a manner similar to hNEN derived from other primary sites on ultrasonography , so it' s difficult to identify in ultrasound . The ultrasonographic features of hNEC are more resemble to malignancy and it can be distinguished from hNET .