It was found that a large amount of malonyldialdehyde ( MDA ) was formed in the burnt skin after 20% TBSA full thickness burns was inflicted to rats. The re were 2 peaks of the increase of MDA on the 2nd and 7th day postburn in the skin respectively. The content of MDA in the plasma and erythrocytes also increased and reached the peak on the 3rd day after injury. The content of vitamin E in the plasma and erythrocytes decreased rapidly from the 2nd day postburn and reached the lowest point on the 3rd day- Hemolysis was found to be the severest on the 3rd day postbarn. After the relationship between the MDA levels in the burnt skin, plasma, and erythrocytes, the vitamin E levels in the plasma and ery-throcyles, and the severity of hemolysis was analyzed, it was found that there was different correlation rale between these parameters in different time-phases, but the general rule was that when MDA increased in the burnt skin, plasma and erythrocytes, vitamin E decreased in the plasma and erythrocytes and hemolysis became more severe. On the basis of these findings, the mechanism of erythrocyte damage after burn injury was discussed.

Hepatobiliary surgical intervention technique is a treatment and diagnostic technology developed relatively rapid in recent years,it is responsible for diagnosis and treatment of important organs such as hepatobiliary pancreatic disease,and with characteristics of minimally invasive,video,safe,simple,quick recovery,less complications,patients easily accepted and so is widely used in clinical.With the popularization and rapid development of this technology,the demand for physician intervention greatly increased,setting off a wave of interventional study.Our hospital has carried out hepatic artery embolization imaging technology and chemotherapy,interventional radiology treatment of biliary tract,portal vein involvement of hepatobiliary surgical intervention since 1980s,the cumulative number of cases of hepatobiliary surgical intervention reached million and attracted many national hospitals to send officers to learn the technology.In the teaching process,we accumulated some valuable experience.

0.05).The gene frequencies of ACG-T235M and ACE-DD plus AGT-TT were significantly higher in the patients with CAD than in the controls respectively(all P

Objective To investigate the feasibility of repairing spinal tracts with peripheral nerve graft combing neurotrophic factors in rats following complete spinal cord transection.Methods One hundred and twenty-one male Wistar Rats were transection at T9 level of spinal cord, and randomly divided into five groups. Group A with spinal cord transection was underwent acidic fibroblast growth factor (aFGF) treatment and peripheral nerve grafts (n=25); Group B: spinal cord transection was underwent aFGF treatment only (n=25); Group C: spinal cord transection was underwent peripheral nerve grafts only (n=25); Group D: spinal cord transaction only (n=25); and Group E: sham control (laminectomy only, n=21). The locomotor behavior of all rats was analyzed by the BBB open field locomotor test over the six months of survival time. Motor evoked potentials (MEP) were used to evaluate axon growth across the damage site. Biotinylated Dextran Amine (BDA) and retrograde tracing with fluorogold were used to evaluate the presence of axons through the damage site after treatment. Results The presence of anterograde BDA labeling of corticospinal tract axons at the graft site and fluorogold retrograde labeling of neuron populations was found in motor cortex and in red nucleus, reticulospinal nuclei, raphe nuclei, and vestibular nuclei in Group A. The average latency and amplitude of MEP were improved significantly in Group C. The mean of BBB scores showed significant improvement in Group A. Statistical analysis indicated that Group A had significant improvement compared to Group BC and D at 6 months post-surgery (P

Mitogen activated protein kinase(MAPK) is one of the four biggest signal transduction systems which contain four subtribes named p38,ERK5/BMK1,ERK and JNK/SAPKrespectively.Previous studies have shown that MAPK pathway is involved in growth,cell differentiation,perishing,the synchronization of cell function and so on.p38MAPK,one of the members of MAPK family,plays an important role in the activation of inflammation-related cells to release inflammation mediator,modulating enzyme production as well as transferring factors activity in the process of chronic bronchitis(CB).This review focuses on multiple roles of p38MAPK in the pathogenesis and progression of CB.

BACKGROUND:The correct method selected according to the specific type and characteristics of lumbar disc herniation can achieve satisfactory effect, but in clinic, there stil some patients have recurrence symptoms after treatment. OBJECTIVE:To investigate the recurrence factors of spinal implant internal fixation for the treatment of lumbar disc herniation, as wel as the intervention measures. METHODS:Fifty-two patients with recurrence symptoms of lumbar disc herniation after internal fixation from January 2002 to December 2007 in the Department of Orthopedics, the 306th Hospital of PLA were retrospectively analyzed, including 28 male cases and 24 female cases, the average age was 43.2 years, ranged from 25-52 years. The time form first internal fixation to the recurrence was 3-192 months, average 38 months. The patients had the symptoms of lumbar and bilateral/unilateral leg pain and numbness. Al the patients underwent the anteroposterior radiographs of lumbar spine and the hyperextension and flexion lateral X-ray films to evaluate the stability of the lumbar spine. Lumbar magnetic resonance imaging was used to observe the intervertebral disc situation of the segment treated with lumbar internal fixation as wel as the spinal stenosis and disc degeneration. RESUTLS AND CONCLUSION:Among the 52 patients, 22 cases had recurrence lumbar disc herniation on the same segment after first internal fixation;four cases had recurrence symptoms caused by the spondylodiscitis after implant internal fixation;five cases had scar adhesions caused nerve compression on the site treated with internal fixation;10 cases had the symptoms of lateral recess and nerve root canal stenosis on the site treated with internal fixation;11 cases had recurrence symptoms caused by lumbar instability after internal fixation. It has reality clinical significance to analyze the reasons of recurrence of lumbar disc herniation after internal fixation

Aim To investigate the effect of TAL and MAPK signal transduction on alveolar macrophage (AM) apoptosis and activity of chronic bronchitis (CB) rats.Methods CB model was established by BCG+LPS method and the in vitro and in vivo experiments were used. MTT method was used to detect the AM activity,and the apoptosis of AM was observed by electron microscope.Results The number of AM in BALF of CB rats was increased than that of normal group (P<0.01).The activity of AM was increased in model group than in control group.The apoptotic rate of AM in CB group was much lower than that in the control group [(13.93±3.34)% vs (5.37±1.38)%] (P<0.01).ERK inhibitor PD98059 induced the apoptosis of cultured AM while JNK inhibitor Curcumin reduced the apoptosis.TAL could inhibit ERK MAPK phosphorylation in AM of CB rats.Further investigation showed that Bcl-2 protein expression was significantly increased while Bax evidently decreased in AM of CB rats.TAL could significantly decrease Bcl-2 expression and increase Bax protein expression, which might be the mechanism of its effect.Conclusions There is an increased activity and decreased apoptosis of AM in CB rats compared with normal rats. TAL can inhibit AM activity and increase apoptosis of AM in CB rats which may be related to the therapeutic effect of CB. ERK and JNK MAPK signal transduction participates in the apoptosis of AM. Regulation of Bcl-2/Bax imbalance and MAPK phosphorylation in AM of CB rats might be the mechanism of its effect.

Objective To observe the growth and biological features of bone mesenchymal stem cells (BMSC) from psoriatic patients. Methods Peripheral blood mononuclear cells were isolated from 5 patients with active psoriasis vulgaris and 5 normal human controls, and BMSC were obtained and purified using plastic adherence method followed by primary culture and passage in vitro. The cell morphology, density and growth were observed with microscopy. Cell growth pattern was evaluated by MTT assay. Flow cytometry was applied to identify surface antigens, including CD29, CD34, CD45 and CD106, on these cells. Results No significant difference was observed in the morphology of primary or descendant BMSC between the patients and controls.The primary BMSC from psoriatic patients tended to adhere to the plastic wall later, confluence and grow more slowly compared with those from the controls. The BMSCs from both psoriatic patients anti healthy donors were positive for CD29, but negative for CD34 or CD45. On the 4th day of culture, the BMSC from psoriatic patients exhibited a decrease in proliferation, with the absorbence at 470 nm (A470) being 0.081±0.0066 and 0.095±0.0130, respectively for BMSC from the patients and controls (t=2.358, P<0.05). Conclusion There is a decrease in the proliferation of BMSC from psoriatic patients which show a morphological similarity to those from healthy controls.

Aim To investigate the effects of cell pro-liferation and activation in HSC-T6 cells by inhibiting the expression of EZH2 , and its partial relevant mech-anism. Methods By introducing the inhibitor DZNep in activated HSC-T6 cells stimulated by TGF-β1 , the protein expression levels of EZH2, p-ERK, p-AKT andα-SMA were detected by Western blot. The siRNA targeting EZH2 was designed and synthesized according to its nucleotide sequence, and their corresponding ex-pression vectors were constructed and transfected into HSC-T6 cells with LipofectamineTM 2000. The prolifer-ation of HSC-T6 cells was determined by MTT. And the protein expression levels of EZH2, p-ERK, p-AKT and α-SMA were measured by Western blot. Results By introducing the inhibitor DZNep in activated HSC-T6 cells stimulated by TGF-β1 , it effectively de-creased the protein levels of EZH2 and also the protein levels of p-ERK, p-AKT and α-SMA. By introducing EZH2-siRNA in activated HSC-T6 cells, it effectively inhibited the cell proliferation, and also the protein levels of EZH2, p-ERK, p-AKT andα-SMA. Conclu-sion Silencing EZH2 expression inhibits HSC-T6 cell proliferation and activation, and EZH2 may be a poten-tial therapeutic target gene for hepatic fibrosis.

Objective To study the subcutaneous injection of physostigmine (PHY) inducing hypothermic response and its relationship with endogenous arginine vasopressin ( AVP). Methods Core temperature and motor activity were monitored by telemetry in female rats maintained at an ambient temperature of 25℃. Tail skin temperature was measured at 30 min intervals with digital thermometer. The central cholinergic antagonist, scopolamine ( lmg/kg ) and AVP V_1 receptor antagonist were administered during the period of PHY (0. 25mg/kg) induced hypothermia. Plasma AVP concentration was measured at 50 min after administration of PHY. Results Subcutaneous injection of PHY led to a rapid reduction in core temperature concomitant with a marked elevation in the heat loss from the tail. The hypothermic response of PHY was blocked by scopolamine and AVP V1 antagonist. Plasma AVP concentration increased markedly at 50 min after PHY. Conclusion The results suggest that endogenous A VP could be involved in PHY -induced hypothermic processes. This may be a novel mechanisms of action for a reversible anticholinesterase drug ( such as PHY ) - induced hypothermia.